European reference method of in vivo determination of metabolisable energy in poultry: reproducibility, effect of age, comparison with predicted values

1. Apparent metabolisable energy corrected to zero-nitrogen retention (AMEn) was measured using the European reference procedure in 9 European laboratories. Seven pelleted diets were evaluated. AMEn was measured with adult cockerels and young birds. 2. Between-laboratory standard deviations were for dry matter (DM) 12.5 g/kg, gross energy 0.085 MJ/kg DM, Kjeldahl nitrogen 0.768 g/kg DM, AMEn (adults) 0.256 MJ/kg DM, and AMEn (young) 0.337 MJ/kg DM. Corresponding coefficients of variation between laboratories were 1.42, 0.45, 2.15, 1.88 and 2.60% respectively. 3. AMEn values of experimental diets were always significantly lower when measured with young birds. This effect was more pronounced when diets contained added fat. 4. Tallow or soyabean oil were incorporated into two basal diets at 40 g/kg inclusion rate. No significant differences were observed between AMEn values of either diet with adults or young birds, suggesting that AMEn energy values of these fats are indistinguishable at this inclusion rate. 5. Similar AMEn values were obtained in young birds by estimating nitrogen accretion, either by the difference between intake and excreta nitrogen or by measuring body weight gain. Determined AMEn values were very similar to those predicted using three different regression equations.     

青脚麻肉鸡对不同来源玉米的代谢能值及近红外预测模型的构建

本试验旨在评定青脚麻肉鸡对30个不同来源玉米的代谢能值,并利用近红外光谱技术构建代谢能值的预测模型,为构建青脚麻肉鸡饲料营养价值数据库和玉米代谢能值的快速预测积累基础数据。试验采用单因素完全随机设计,选用48只体重相近的30周龄青脚麻肉公鸡,按照组间体重无差异原则随机分组,做9批次代谢试验,每批做3或4个玉米样,每样设8个重复,每个重复1只鸡;每批做1个内源组,每批之间设10 d恢复期。试验采用排空强饲法测定代谢能值,试鸡饥饿48 h,然后按体重2%强饲待测饲料,收集排泄物48 h;内源组鸡饥饿48 h,再继续饥饿收集排泄物48 h。结果显示:1)30个玉米样品的干物质含量为(86.75±0.55)%(85.55%~87.79%),以干物质为基础,粗蛋白质含量为(9.21±0.52)%(8.27%~10.58%),总能为(18.716±0.106)MJ/kg(18.429~18.951 MJ/kg),中性洗涤纤维含量为(13.00±2.21)%(10.00%~18.52%),酸性洗涤纤维含量为(3.23±0.46)%(2.37%~4.36%),粗纤维含量为(2.28±0.28)%(1.89%~2.76%)。2)以干物质为基础,青脚麻肉鸡对30种玉米的表观代谢能(AME)为(14.627±0.655)MJ/kg(11.727~16.225 MJ/kg),氮校正表观代谢能(AMEn)为(14.672±0.641)MJ/kg(11.793~16.248 MJ/kg),真代谢能(TME)为(16.248±0.619)MJ/kg(13.333~17.727 MJ/kg),氮校正真代谢能(TMEn)为(16.293±0.605)MJ/kg(13.398~17.750 MJ/kg)。3)用近红外光谱技术建立的青脚麻肉鸡AME、AMEn、TME、TMEn校正决定系数(R2cal)、校正标准差(RMSEE)及相对标准差(RSD)分别为0.99、0.035、0.24,0.99、0.029、0.20,0.99、0.031、0.19,0.99、0.030、0.18;交叉验证决定细数(R2cv)、交叉验证标准差(RMSECV)及RSD分别为0.92、0.117、0.80,0.93、0.106、0.73,0.90、0.113、0.70,0.91、0.108、0.66。结果表明:1)青脚麻肉鸡对不同来源玉米的AME、AMEn、TME和TMEn存在差异;2)近红外模型可以较好地预测青脚麻肉鸡的玉米代谢能值。     

The effects of dietary energy concentration on growth rate and conversion of energy to weight gain in broiler chickens

Eight diets, their metabolisable energy (ME) concentrations ranging from 2.3 Mcal/kg to 3.6 Mcal/kg (9.6 to 14.1 MJ/kg) were fed to each of four pens of broilers in two trials. Food intake was inversely related to energy concentration but the ME required by each sex to reach a given live‐weight was quite similar. Birds on the diets with a medium energy concentration (about 3.1 Mcal/kg (13.0MJ/ kg)) required slightly less ME than those on other diets and generally reached the required live‐weight earlier than those on other diets. Birds in the first trial required less of any particular diet than their counterparts in the second trial, reflecting the lower ambient temperatures recorded during the latter trial. Mortality recorded from 14 d of each trial was generally lower for birds on the medium energy concentration diets and dressed carcass percentage higher than those on other diets.

The results of the present study are in agreement with the recent recommendation (National Research Council, 1971) of 3.2 Mcal ME/kg (13.4 MJ/kg) for broiler diets. However, it is suggested that minimum and maximum values be given in such recommendations; this would allow greater flexibility and a wider range of ingredients in diet formulation.     

Effect of soaking,fermentation with lactobacillus and enzyme treatment on nitrogen‐corrected apparent metabolizable energy values of wheat in cockerels and quails

An experiment was carried out to determine nitrogen‐corrected apparent metabolizable energy (AMEn) values of processed wheat products with two levels of enzyme (0 and 0.5 g/kg) and five processing methods [control (C), water‐soaking for 12 h (SO12h), 24 h (SO24h) and 48 h (SO48h) and fermentation with lactobacillus (FL)] in two species of poultry (cockerel and Japanese quail). In this study, each processing method group had six replications with two cockerels or four quails per replication. The results showed that the calculated AMEn values of processed wheat by different methods were as follows: C‐12.61 and 12.60; SO12h‐12.75 and 12.73; SO24h‐12.80 and 12.79; SO48h‐12.97 and 12.96; and FL‐13.36 and 13.33 MJ/kg for cockerels and quails, respectively, indicating that fermentation improved AMEn values compared with the others. The mean AMEn of processed wheat with enzyme (13.12 MJ/kg) was significantly higher than that of processed wheat without enzyme (12.64 MJ/kg). However, there were no significant differences in the AMEn of processed wheat between cockerels and quails. The interaction between species and levels of enzyme in AMEn values was not significant. There was no significant difference (p > 0.05) in three‐way interactions among species, levels of enzyme and wheat processing methods for AMEn values. In conclusion, the results of the experiment suggest that the FL was more effective processing method of wheat to obtain high level of AMEn values than the others, and the combination of enzyme and processing methods can increase the AMEn values compared with processing methods alone.     

Nutritional evaluation of sunflower seed and products derived from them. Effect of oil extraction

1. Apparent MEn and oil digestibility of hulled sunflower seed (SFS) and the products derived from the oil extraction process: press extracted SFS (PESFS), sunflower seed meal (SFSM), and press and solvent oils (PO and SO) were determined with 198 cockerels. Recombined products (mix of meal and oil) were also evaluated to study the effect of the 2 consecutive oil extraction steps. Each foodstuff was included in a basal diet, according to the proportions resulting from processing, at 100, 200, and 300 g SFS/kg. 2. Dietary energy value and digestible fat content were linearly related to rate of inclusion of test ingredients. Extrapolation values for AMEn (MJ/kg DM) were: SFS, 16.20; PESFS, 9.46; SFSM, 7.62. A decreasing quadratic trend was also found in the AMEn of PESFS, with interpolation values ranging from 11.77 to 9.33 MJ/kg DM between 70 and 210 g/kg DM of inclusion. No differences were observed between PO and SO. The AMEn of of sunflower oil, calculated from its digestibility, was 33.70 MJ/kg DM. 3. The oil extraction process affected the nutritional value of sunflower products. Recombined materials showed greater values than original foodstuffs: R-SFS, 17.47; R-PESFS, 11.49 MJ/kg DM. The increase in oil digestibility (from 0.814 to 0.862 g/kg DM in SFS; from 0.778 to 0.892 in PESFS) accounted for most of the increase observed in AMEn values. 4. As the form in which oil is incorporated in diets (released or within SFS or PESFS) affects the utilisation of sunflower products, their nutritional value is less than maximal and should not be calculated from their ingredients. Solvent oil seems to be the less available fraction of sunflower oil within SFS as the effect of the 2nd extraction proved to be greater than that of the previous press extraction.     

东北玉米肉鸡表观代谢能预测方程的建立

本研究旨在通过实验室分析和肉鸡代谢试验建立东北玉米养分含量与代谢能的回归方程,为生产提供便捷的玉米代谢能估算方法。试验1:选取成熟季节不同的20种东北玉米,测定养分含量和容重,分析养分含量变异范围。试验2:用玉米、玉米皮、玉米碴按照中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)含量梯度配制人工玉米,再添加一定量的维生素和矿物质,配制成人工玉米代谢饲粮。选取正常饲养的商品代爱拔益加雄性肉鸡进行代谢试验,分别在11~13日龄及25~27日龄,用全收粪法测定人工玉米代谢饲粮表观代谢能(AME)、氮校正表观代谢能(AMEn)和养分表观消化率,并采用逐步回归法建立AME及AMEn与养分含量的回归方程。结果表明:东北玉米粗脂肪(EE)、NDF及ADF含量变异系数相对较大,分别为16.72%、14.65%、14.72%。肉鸡对人工玉米代谢饲粮的养分表观消化率在11~13日龄和25~27日龄之间存在差异,25~27日龄肉鸡对人工玉米代谢饲粮粗蛋白质(CP)、EE和总淀粉(STC)的表观消化率高于11~13日龄,其中EE表观消化率差异显著(P0.05);25~27日龄肉鸡对人工玉米代谢饲粮的AME、AMEn也显著高于11~13日龄(P0.05)。采用逐步回归法建立肉鸡东北玉米代谢能的预测方程为:11~13日龄,AME=17.661-0.853ADF(R2=0.870,P0.01),AM En=17.468-0.878ADF(R2=0.873,P0.01);25~27日龄,AM E=18.102-0.792ADF(R2=0.781,P0.05),AM En=17.935-0.830ADF(R2=0.784,P0.05)。通过预测方程计算所得人工玉米代谢饲粮AME的预测值与实测值很接近,计算所得的东北玉米AME符合预期值。由此得出,不同品种东北玉米EE、NDF及ADF的含量差异相对较大;肉鸡不同阶段玉米代谢能存在差异,计算肉鸡饲粮配方时,不同阶段饲粮代谢能应采用对应的代谢能值;低于14日龄的肉鸡,东北玉米AM E、AM En预测方程分别为AM E=17.661-0.853ADF,AM En=17.468-0.878ADF;14日龄以上的肉鸡,东北玉米AME、AMEn预测方程分别为AME=18.102-0.792ADF,AMEn=17.935-0.830ADF。     

European reference method for the in vivo determination of metabolisable energy with adult cockerels: reproducibility, effect of food intake and comparison with individual laboratory methods

1. A common reference procedure adopted by several European laboratories to determine apparent metabolisable energy corrected to zero-nitrogen balance (AMEn) is described. 2. Reproducibility has been estimated by comparing results from 4 diets measured in 7 laboratories. The standard deviations between laboratories of dry matter, gross energy, Kjeldahl nitrogen and AMEn were respectively, on average, 11.29 g/kg, 0.242 MJ/kg, 1.56 g/kg and 0.380 MJ/kg dry matter; the corresponding coefficients of variation between laboratories were 1.27, 1.29, 4.39 and 2.92%. 3. Effect of food intake was tested by comparing AMEn from birds fed either 0.90 or 0.45 of ad libitum: the amount of food intake had a negligible effect on AMEn values. Endogenous energy losses corrected (EELn) or not (EEL) to zero nitrogen balance were estimated either by regression between excreted energy and ingested energy or in fasted and glucose-fed cockerels. EELn estimated by regression was, on average, 8.7 kJ/bird/d.; it was independent of diet composition. EEL and EELn determined in fasted or glucose-fed birds were higher than those determined by regression. 4. AMEn values measured by individual laboratory procedures were very close to those obtained by the reference method, except from laboratories using a tube feeding procedure, where deviations were probably a consequence of overestimated EEL.     

Content of plasmatic glycerol and activity of hepatic glycerol kinase in broiler chickens fed diets containing different sources and concentrations of glycerine

The objective of this study was to evaluate the effect of three sources of glycerine (crude glycerine from soya bean oil – CGSO, mixed crude glycerine from frying oil and lard – MCG, and a semipurified glycerine from soya bean oil – SPGSO) in four concentrations in the diet (17.5, 35.0, 52.5 and 70.0 g of each type of glycerine per kg of feed) on the levels of plasmatic glycerol and the activity of the hepatic enzyme glycerol kinase in broilers of 22–35 days old (experiment I) and 33–42 days old (experiment II). The highest (p < 0.05) plasmatic glycerol level was detected in broilers fed diet containing CGSO. Independent of the source, increasing the concentration of glycerine led to a linear increase (p < 0.05) in the plasmatic glycerol concentration. In experiment I, all the diets containing glycerine resulted in increased concentrations of plasmatic glycerol, in relation to the control diet without glycerine. However, in experiment II, only the diet containing 17.5 g of CGSO per kg of feed and the diets formulated with any of the three types of glycerine in the inclusion concentrations of 35, 52.5 and 70 g/kg of feed resulted in higher plasmatic glycerol levels than those observed in the control broilers. The source of glycerine influenced the glycerol kinase activity only in experiment II, where the use of CGSO in the diet increased the enzyme activity. For both experiments, the glycerol kinase activity increased with the inclusion of glycerine in the diet. In conclusion, based on the absence of saturation of the glycerol kinase activity for the three glycerine sources and for both rearing periods evaluated, the broilers can metabolize the glycerol (at the level of the phosphorylation of the glycerol to glycerol‐3‐phosphate in the liver) present in the glycerine when the diet is supplemented with up to 70 g of glycerine per kg of feed.     

生物秸秆对肉鸡表观代谢能的影响及替代玉米适宜比例的研究

本试验旨在研究肉鸡对生物秸秆的表观代谢能及其替代玉米的适宜比例.生物秸秆代谢能的测定试验选择24只42日龄健康的爱拔益加(AA)肉鸡,随机分为3个处理,每个处理8只鸡.处理1饲喂含8％生物秸秆的基础饲粮,处理2饲喂以30％的生物秸秆代替基础饲粮中能量饲料和蛋白质饲料的饲粮,处理3采用强饲法进行测定.生物秸秆代替玉米的试验选择150只42日龄健康的AA肉鸡,随机分为5个处理,每个处理5个重复,每个重复6只鸡.对照组饲喂基础饲粮,试验组分别在基础饲粮中用4％、8％、12％生物秸秆代替玉米(代谢能按照差量法计算结果12.46 MJ/kg计)及8％生物秸秆代替玉米(代谢能按照强饲法计算结果5.30 MJ/kg计,添加豆油,使其代谢能水平与对照组相同).结果表明:1)基础饲粮的代谢能为12.63 MJ/kg,采用差量法推算出肉鸡对生物秸秆的表观代谢能为12.46 MJ/kg,而采用套算法和强饲法测定生物秸秆的表观代谢能分别为4.38和5.30 MJ/kg.2)与对照组相比,4％和8％的生物秸秆代替玉米组中性洗涤纤维和酸性洗涤纤维的代谢率显著升高(P＜0.05),而12％的生物秸秆代替玉米组表观能量的代谢率显著降低(P＜0.05).由此得出,套算法和强饲法低估了生物秸秆的表观代谢能,而差量法更能准确反映生物秸秆的表观代谢能;在肉鸡饲粮中生物秸秆可以替代4％ ～8％的玉米.     

Apparent metabolisable energy and amino acid digestibility of microalgae Spirulina platensis as an ingredient in broiler chicken diets

ABSTRACT

1. This work investigates the apparent metabolisable energy (AME), its nitrogen-corrected form (AMEn) and amino acid (AA) digestibility coefficients of the microalgae Spirulina platensis as an ingredient in broiler chicken diets.

2. One group of birds was fed with a basal control diet (BD), and another was fed with a test diet composed of the BD with the addition of the microalgae at a proportion of 200 g/kg. AME and AMEn were assessed by total excreta collection and indigestibility analysis using acid-insoluble ash (AIA) as a marker.

3. The microalgae comprised 888.0 g/kg dry matter (DM), 18.42 MJ/kg gross energy, 514.7 g/kg crude protein, 9.9 g/kg ether extract, 10.6 g/kg crude fibre, 94.4 g/kg ash, 3.1 g/kg Ca and 11.0 g/kg P.

4. The values obtained for AME (13.48 MJ/kg DM) and AMEn (11.72 MJ/kg DM) were higher (p < 0.01) than those obtained using the AIA method (9.39–8.29 MJ/kg DM). The microalgae ileal digestibility coefficients were 0.80 ± 0.04 and 0.78 ± 0.04 for essential and non-essential AA, respectively.

5. Overall, the metabolisable energy content and digestibility of AA for S. platensis indicated that the microalgae are potentially an alternative nutrient source for broilers. The AIA method underestimated AME and AMEn in comparison to the total excreta collection method.     

饲粮添加大豆卵磷脂乳化剂对肉鸡生长性能、养分利用率和血清生化指标的影响

本试验旨在研究在2种能量水平饲粮中添加大豆卵磷脂乳化剂对肉鸡生长性能、养分利用率和血清生化指标的影响。试验采用2×2因子完全随机区组试验设计,将960只1日龄科宝500白羽肉公鸡随机分为4组,每组6个重复,每个重复40只鸡。各组试验鸡分别饲喂2个能量水平和2个乳化剂添加水平组成的试验饲粮,其中代谢能分别为12.13和11.92 MJ/kg(1～21日龄)、12.96和12.65 MJ/kg(22～49日龄),乳化剂添加水平分别为0和265 mg/kg(1～21日龄)、0和400 mg/kg(22～49日龄)。试验测定肉鸡生长性能,能量、干物质、粗蛋白质和粗脂肪表观利用率,以及血糖、血脂和胆汁酸含量。结果表明:肉鸡采食正常能量饲粮前期(1～21日龄)平均日增重极显著高于低能量饲粮(P<0.01),肉鸡全期(1～49日龄)采食添加乳化剂的低能量饲粮料重比能达到正常能量饲粮水平;饲粮添加乳化剂有提高平均日增重和饲粮粗脂肪和能量表观利用率的趋势,可促进胆汁分泌,能显著降低前期及全期料重比(P<0.05)。结果显示,降低饲粮能量显著降低1～21日龄肉鸡平均日增重,提高1～21日龄和1～49日龄料重比;饲粮添加乳化剂能够改善饲料利用率,特别是在1～21日龄肉鸡低能量饲粮中效果更明显。     

不同日粮对广西合浦鹅生产性能及养分代谢的影响

本研究旨在探讨不同日粮对鹅生长性能和纤维类物质及其他养分利用能力的影响。选取4周龄、体重相近的广西合浦鹅60只,随机分为3组,每组20只(公母各1/2),分别饲喂3种不同代谢能(ME)水平的日粮:第1组10.03 MJ/kg,第2组11.00 MJ/kg,第3组11.85 MJ/kg,进行42 d的饲养试验;同时分别于5周龄和9周龄时从上述3组试验鹅中各选取4只体重相近的试鹅进行代谢试验并用内源指示剂法(4 mol/L HCl不溶灰分)测定试鹅对日粮养分的代谢率。结果表明:在整个试验期间各组试鹅平均日增重差异不显著(P>0.05);5周龄时,各组日粮养分代谢率差异也不显著(P>0.05);但在9周龄时,第1组的CF、DM和能量代谢率显著高于其余2组(P<0.05或P<0.01)。因此,本试验证明了日粮ME在10.03~11.85 MJ/kg时,不影响合浦鹅的增重和屠宰性能;在9周龄、日粮ME为10.03 MJ/kg时,其CF、DM和能量代谢率显著高于ME为11.00或11.85 MJ/kg组,而高能量日粮并未表现出养分代谢上的优势。     

日粮不同能量水平对育成鸡体增质量、血液生化指标及内脏器官发育的影晌

选用60日龄体质量均匀、健康的塞北乌骨鸡120只,随机分为I～V个试验组,每个试验组设4个重复,每个组6只乌骨鸡（雌雄各半）,探讨饲粮中添加不同水平能量（10．38,10．94,11．51,12．34,12．96MJ／kg）,对塞北乌骨鸡体增质量、血液指标和免疫器官的影响。结果表明。育成期公鸡在12．34MJ／kg时体增质量最高,母鸡在12．96MJ／kg时体增质量最高;80日龄时公鸡调节血糖的能力优于母鸡,对肝脏具有很好的保护力;LDL—C与日粮能量引起体质量的变化具有一定的相关性。总胆固醇的含量在育成期随着日粮中能量水平的增加而逐渐下降。日粮能量水平不同,对内脏器官的影响也不同,都存在不同的显著性差异。     

14个小麦样品的肉鸡代谢能评定

本试验采用26～31日龄的AA肉仔鸡测定了小麦的表观代谢能(AME)、氮校正表观代谢能(AMEn)和回肠表观消化能(IDE),比较了3种生物学方法所得小麦表观有效能值之间的差异,分析了不同小麦表观有效能值对饲料配方原料选择的影响。结果表明:全收粪法和指示剂法测得14种小麦的AME平均值分别为13.27MJ/kg和12.64MJ/kg,回肠食糜法测得的IDE平均值为12.05MJ/kg,全收粪法和指标剂法测得小麦的AMEn平均值分别为12.95MJ/kg和12.01MJ/kg;不同产地小麦和3种生物学方法所得的小麦表观有效能值对配方计算结果均能产生显著影响。     

日粮直/支链淀粉比对黄羽肉仔鸡生产性能和养分利用率的影响

本文旨在研究不同日粮直/支链淀粉比对黄羽肉仔鸡生产性能及养分利用率的影响.试验采用单因子设计,选用29日龄黄羽肉仔鸡320只,随机分为4个处理,每处理设5个重复,每重复16只鸡,分别饲喂直/支链淀粉比为0.11、0.23、0.35、0.47的试验日粮.试验为期28 d,测定了肉仔鸡生产性能和饲料养分消化率,结果表明:日粮直/支链淀粉比影响动物的生产性能和饲料养分利用率,试鸡平均日采食量直/支链淀粉比为0.11的日粮组显著高于其他各处理(P<0.05),但料重比却以直/支链淀粉比为0.23的日粮组最低(P<0.05);日粮各氨基酸的消化率除苏氨酸、蛋氨酸和赖氨酸直/支链淀粉比0.23日粮组略低于直/支链淀粉比0.11的日粮组外,其他各氨基酸均以直/支链淀粉比0.23日粮组最高,其次是直/支链淀粉比0.11、0.35和0.47日粮组;日粮代谢能值也以直/支链淀粉比0.23日粮组最高,AME、AMEn、TME、TMEn值分别为15.20、14.70、15.60和15.04 MJ/kg,比直/支链淀粉比为0.11、0.35、0.47的日粮组高7.80%、2.70%、6.37%;7.14%、3.45%、7.30%;7.22%、3.31%、6.78%和6.21%、3.65%、7.28%(P<0.05).     

能量水平对0～21日龄岭南黄肉鸡生产性能、胴体品质和体组成的影响

将600只岭南黄初生雏鸡随机分为5组,每组120只,公母各半,分栏饲养,饲粮代谢能水平(MJ／kg)分别为11.88,12.19,12.92,13.22和13.60,观察21天。结果,11.88 MJ／kg组公雏平均日增重显著低于其余四组(p<0.05),料肉比显著高于其余四组(p<0.05),13.60MJ／kg组腹脂率最高,极显著高于其余四组(p<0.01),13.22和13.60MJ／kg组体蛋白含量极显著低于11.88,12.19MJ/kg组(p<0.01);对母雏,饲粮代谢能12.92,13.22和13.60MJ／kg组平均日增重显著或极显著高于11.88,12.19MJ／kg组(p<0.05或P<0.01),料肉比显著低于11.88 MJ／kg组(P<0.05),11.88 MJ／kg组体脂肪含量显著低于其余四组(p<0.05),但体蛋白含量显著高于其余四组(p<0.05),灰分含量极显著高于12.92,13.22和13.60MJ／kg组(p<0.01)。     

Cell wall content as a predictor of metabolisable energy value of poultry feedingstuffs

1. Forty eight diets were tested to develop equations for predicting the apparent metabolisable energy values corrected to zero nitrogen retention (AMEn), of practical poultry feedingstuffs.

2. The effectiveness of different fibre indicators (crude fibre, acid detergent fibre, lignin, neutral detergent fibre and cell wall content) as predictors of AMEn values was assessed. The most efficient measure proved to be cell wall content obtained by an enzymatic procedure (pronase and α‐amylase).

3. The best combinations of variables were, in decreasing order: (1) gross energy (GE), crude protein (CP) and cell wall (CW); (2) ether extract (EE), ash and CW; (3) EE, CP, starch and sugars.

4. When GE, CP and CW variables were used, a significant constant appeared in the linear equation. Another equation without a constant was calculated, using an exponent (1·5) for the CW variable. The residual standard deviation (0·20 MJ/kg) of this equation was about the same as that of the equation with the constant.

     

Combined effect of using near‐infrared spectroscopy for nutritional evaluation of feed ingredients and non‐starch polysaccharide carbohydrase complex on performance of broiler chickens

This study was carried out to evaluate the combined effect of using near‐infrared spectroscopy (NIRS ) for nutritional evaluation of feed ingredients and the addition of non‐starch polysaccharide carbohydrase complex (NSP enzymes) on the growth performance of broilers fed diets produced with low‐quality wheat and soybean meal. A 2 × 2 trial design was performed, with seven replicates of 40 male Ross 308 broilers per treatment, evaluating the effect of the addition of NSP enzymes and the ingredients’ nutritional matrix based on table values or NIRS values. Diets without added enzymes were formulated to reach nutritional requirements, whereas diets with enzymes were reformulated, reducing the apparent metabolizable energy (AME ) by 85 kcal/kg. In the overall period (days 0–35), broilers fed diets formulated using NIRS values had higher (P  < 0.001) average daily gain (+11.3%) and daily feed intake (+7.2%), and a lower (P  < 0.001) feed conversion ratio (?5.3%) compared to those fed diets formulated using table values. When formulating diets for broilers with low‐quality feed ingredients, performance can be improved by considering NIRS values and by the addition of NSP enzymes, even with a reduction of AME . These nutritional approaches are efficient in improving broilers’ performances by themselves and even more so when they are combined.     

肉鸡能量需要动态模型的建立和参数估计

本研究通过两个试验评价肉仔鸡的能量需要。试验1选用36只AA肉仔鸡新生雏笼养6周,分别于3周龄和6周龄评定2种饲粮的表观代谢能(AME)和表观氮校正代谢能(AMEn)。结果表明:氮校正使AME降低5%左右,AMEn和AME的相关性较强。试验2选用AA肉鸡新生雏240只,公母分饲笼养6周,每周测定空腹体重及采食量。用Compertz方程拟合公母鸡的生长曲线,微分建立日增重与日龄的函数关系,再与利用一元线性回归法建立的能量需要模型组合,形成动态的能量需要预测机制。     

半细毛羊5种玉米的可消化粗蛋白质和有效能的测定及其预测

本试验旨在采用概略养分分析法测定5种玉米的营养成分含量以及通过消化代谢试验实测玉米的可消化粗蛋白质含量和有效能值,并利用先采集的4种玉米(奥美特、杰尼336、巨丰66、京科665)数据拟合可消化粗蛋白质、消化能和代谢能分别与营养成分之间的回归方程,然后利用第5种玉米(禾育九)可消化粗蛋白质和有效能数据来对回归方程进行验证和修正。选取16只体重为(56.44±5.11)kg的云南半细毛羊,采用完全随机设计,平均分为4组,每组4只。试验共分2期进行,共6种日粮,分别是基础日粮和5种试验日粮,第1期饲喂4种日粮,第2期饲喂2种日粮。结果表明:京科665的可消化粗蛋白质(DCP)、消化能(DE)和代谢能(ME)含量最高,杰尼336的DCP、DE和ME含量最低;玉米的DE和ME分别与EE呈极显著负相关关系(P<0.01),相关系数分别为-0.985和-0.994;试验所得的预测方程为DCP(g/kg)=258.961-52.624EE(粗脂肪)、DE(MJ/kg)=40.506-6.876EE、DE(MJ/kg)=34.677-6.398EE+0.492CP(粗蛋白质)、DE(MJ/kg)=-115.182-6.572EE+0.473CP+1.525OM(有机物)、ME(MJ/kg)=-1.028+0.945DE、ME(MJ/kg)=2.950+0.916DE-1.060CF(粗纤维)和ME(MJ/kg)=37.586-6.586EE。综合DCP含量和有效能值,京科665的品质最佳,其次是巨丰66、奥美特和禾育九,杰尼336品质最差;以半细毛羊为试验对象得出,可以利用玉米的营养成分预测其DCP含量以及有效能值。