Matching grass supply to grazing patterns for dairy cows

Four groups of five spring-calving Holstein–Friesian cows were given a daily grass allowance in a strip-grazing system after either morning (AM; two groups) or afternoon (PM; two groups) milking over a 10-week period. The dry-matter (DM) concentration of the grass tended to be higher after afternoon milking [AM, 178 vs. PM, 197 g DM (s.e.d. 5·32) kg^–1 fresh matter], and water-soluble carbohydrate concentrations were significantly higher [AM, 175 vs. PM, 204 g kg^–1 DM (s.e.d. 6·67)]. Although the total times spent grazing (AM, 461 vs. PM, 462 min day^–1) were similar for both groups, cows receiving their allocation in the afternoon had a longer evening meal (>4 h duration) compared with those receiving their allocation in the morning (2–3 h), which also spent more time ruminating [AM, 454 vs. PM, 433 min day^–1 (s.e.d. 1·80)]. This short-term study demonstrated how a relatively simple change in management practice in strip-grazing systems could benefit milk yield and pasture utilization. This is because the DM and water-soluble carbohydrate contents of the herbage are higher in the evening than in the morning, and this is when grazing animals concentrate much of their daily grazing activity.     

A comparison of buffer and partial storage feeding of a straw/concentrate mixture to grazing dairy cows

A straw/concentrate mixture was offered to set-stocked dairy cows over a 24-week period. The cows were offered grazed herbage only (G), or grazed herbage with a straw/concentrate supplement offered either for 45 min after each milking (B), or overnight (P). The overnight treatment involved housing the cows between afternoon and morning milking. The straw/concentrate mixture contained 0·33 long barley straw, 0·28 barley, 0·12 soya bean meal, 0·25 molaferm and 0·02 minerals. During the first 8 weeks of the experiment an average of 2·25 kg of concentrate were fed, and from weeks 9–24, 2·0 kg of concentrate were fed.
The feeding of the straw/concentrate mixture led to a decrease in estimated herbage dry matter (DM) intake, particularly for treatment P. Estimated total DM intakes were increased throughout the experiment by offering the straw/concentrate mixture. However, total metabolizable energy (ME) intakes were only increased in mid-and late season.
Milk yield was higher in early season for treatment G; 28·1 kg d⁻¹ compared to 26·8 kg d⁻¹ and 25·5 kg d⁻¹ for treatments B and P respectively. In late season the cows in treatment G had lower milk yields; 13·3 kg d⁻¹ compared to 15·5 kg d⁻¹ and 16·8 kg d⁻¹ for treatments B and P respectively. Milk fat content was increased in early season in treatment P, and milk protein content tended to be reduced throughout the experiment for cows offered the straw/concentrate mixture overnight. Over the whole experiment there were no differences in yield of milk solids.     

How does protein supplementation affect the selectivity and performance of Charolais cows on extensively grazed pastures in late autumn?

Management of beef cows grazing extensively grazed semi-natural pastures in temperate regions in late autumn can require supplements to be offered. The effects of supplementation with soya bean meal on the diet selected by Charolais cows and on their subsequent performance were examined for an 8-week period in late autumn in 2 years. Three groups of eight cows were compared: non-supplemented dry cows (D), non-supplemented (L) and supplemented (LS) lactating cows. The amount of soya bean meal supplement offered per cow was 250 g d⁻¹ in year 1 and 800 g d⁻¹ in year 2. Dietary choices were measured by direct observations and herbage intake was estimated in year 2. Supplementation affected neither diet selection (L: 0·42 vs. LS: 0·43 for the proportion of bites on green patches in year 1; 0·24 vs. 0·22 in year 2) nor daily organic matter intake of herbage (L: 15·6 vs. LS: 15·9 kg d⁻¹), which may have resulted from an adequate crude protein concentration of herbage. The higher total dry matter intake by cows offered the supplement reduced losses in live weight (L: −1212 vs. LS: −828 g d⁻¹; P  < 0·01) rather than increased milk production (L: 5·1 vs. LS: 5·0 kg d⁻¹). This may be linked to the low milk yield potential of the Charolais cows. The use of lactating cows rather than dry cows for pasture management in late autumn would increase the utilization of herbage but a reduction in liveweight losses of cows by supplementation is unlikely to be economic.     

A COMPARISON OF NUTRIENT LOSSES IN BALED HAY MADE FROM FORAGE-HARVESTED, CRIMPED OR TEDDED SWARDS WITH THOSE IN BARN-DRYING

Two experiments were conducted to study the effect on nutrient loss and feeding value of making hay by different means, using a barn-drier, a forage-harvester, a crimper and conventional tedding. Both forage-harvesting and crimping of the herbage significantly increased the rate of drying, compared with tedding alone. In Exp. 2 this effect enabled the crimped and forage-harvested hays to be baled for storage 3 days earlier than the tedded hay. The use of the forage-harvester significantly reduced the yield of herbage compared with conventional mowing. Nutrient losses from cutting until storage were highest for the forage-harvested hays, the loss of dry matter between cutting and storage in the forage-harvested hays being 39·9 and 19·3%, respectively, in Expt 1 and 2 with corresponding losses in the conventionally made hays of 7·1% and 9·3%. Crimping the hay resulted in higher nutrient losses than tedding. In Expt 2 there was a loss of dry matter of 17·5% in the conventionally made hay during an 18-week storage period, compared with 3·6% in barn-dried hay, 10·8% in forage-harvested hay and 8·8% in crimped hay.
In Expt 2, in which digestibility determinations were made, conservation decreased the total digestible nutrients and starch equivalent in all treatments. The use of the barn-drier gave the most efficient conservation of total digestible nutrients, followed by the crimped, forage-harvested and conventional treatments in that order. The starch equivalent values of hays made from the same sward were 43·0 when barn-dried, 39·8 when crimped, 38·6 after forage-harvesting and 31·6 after tedding.     

Some characteristics of reduced leaf photosynthesis at midday in maize growing in the field

Maize was grown in the high-radiation arid summer environment of Davis, California, and its leaf photosynthetic rate was measured over diurnal courses on cloudless days with the leaf held perpendicular to the sunlight. On days of high atmospheric vapor pressure deficit (VPD), leaf photosynthesis reached a maximum in the late morning and then decreased gradually as the day progressed, though the soil was well irrigated. When CO₂ concentration in the measurement chamber was raised to about 1000 μmol mol⁻¹, photosynthesis was enhanced, but more in the afternoon than in the morning. As a result, rates measured at high CO₂ in the morning and afternoon were essentially the same. There was also no difference in the curves of photosynthetic rate (A) versus intercellular CO₂ concentration (C_i) for the morning and afternoon. Hence, photosynthetic capacity was similar for the two periods and there was no evidence of photoinhibition by the high photosynthetic photon flux density at noon. Further, C_i and photosynthetic rates A measured over a range of photon flux density were lower in the afternoon than in the morning. These results indicate that A at noon and early afternoon was more limited than in the morning by epidermal conductance (mostly stomatal). On a day of low VPD, however, midday depression in A and epidermal conductance were not evident for the well-irrigated plants. Without irrigation and with leaves at a lower midday water potential, midday reduction in conductance and A was much more marked, beginning late in the morning. Epidermal conductance of maize grown in the field in Davis is are not sensitive to VPD. Therefore, the midday reduction in conductance and A was more likely the result of low leaf water potential caused by high transpiration rates.     

The nitrogen value of anaerobically digested sewage sludge applied to grassland during winter

The manurial value of liquid anaerobically digested sewage sludge (LAS), surface applied once at four different times during winter to contrasting swards, was assessed over each of three growing seasons by comparison with fertilizer N. Four nominal rates of N (0, 18, 36 and 54 kg ha⁻¹) were used based on the inorganic N content of LAS. Herbage dry matter (DM) and N uptake responses were measured by cutting three times per year.
Application of LAS in late winter compared with early winter led generally to greater N uptake and DM production. Herbage production response from LAS ranged from 5·4 to 30·1 kg DM kg⁻¹ N; within this range, larger responses were obtained from a sown sward than from permanent pasture. The apparent efficiency of LAS compared with fertilizer N ranged from 0·41 to 0·88 (permanent pasture) and 0·20 to 0·77 (sown sward) but occasionally mean apparent efficiencies of over 1·0 occurred because fertilizer N was poorly recovered. There was no residual effect on annual N uptake from applying sludge. It is concluded LAS is a useful nitrogenous manure when applied to grassland in late winter/early spring but more research is needed to elucidate the importance of pathways of N losses following the surface application of LAS, and to quantify these losses.     

Supplementary feeding of forage to grazing dairy cows, 3. The effect of three daytime stocking rates on the performance of cows offered grass silage overnight

Over a 24-week period, three groups of dairy cows were continuously stocked at 8, 10 or 12 cows ha^-1 between morning and afternoon milkings, and overnight were housed and offered grass silage ad libitum. Due to a prolonged drought, sward heights only averaged 4·1 cm.
The increase in daytime stocking rate led to a decline in herbage intake, and increases in silage intake. At the highest stocking rate (12 cows ha^-1), the silage intake failed to compensate for the reduced herbage intake. Consequently the total dry matter and estimated metabolizable energy intakes were lower than for the 8 and 10 cows ha^-1 treatments. Milk yields and milk composition were not significantly affected by treatment but the 12 cows ha^-1 stocking rate gave the lowest milk and milk solids yields.
The utilized metabolizable energy (UME) on the grazed swards was greatest for the 10 cows ha^-1 treatment. The sward cut to provide the silage had a UME level (GJ ha^-1) 32% greater on average than the grazed swards during the same growth period. The total areas utilized for grazing and silage production for 8, 10 and 12 cows ha^-1 were 0·240, 0·224 and 0·215 ha respectively. Fat and protein yields per unit area were greatest for the 10 cows ha^-1 group.     

LOSSES IN THE CONSERVATION OF HEAVILY-WILTED HERBAGE SEALED IN POLYTHENE FILM IN LINED TRENCH SILOS

A conservation experiment is described in which the losses in ensiling heavily-wilted herbage, of approximately 50% dry matter, in lined trench silos, with and without a polyethylene film seal, are compared. The composition and digestibility of the silages produced in the sealed silos were comparable with those of the original herbage. The content of organic acids in the sealed silos was low, suggesting that fermentation was suppressed by the partially gas-tight seal. In the unsealed silos there was considerable spoilage, 70% of the material being inedible. In the 2 sealed silos losses of dry matter were 8·2 and 5·2% and losses of SE 11·5 and 7·8%, respectively. The losses recorded are compared with those usually encountered in lined trench silos.     

大豆杂交中伪杂种产生的原因分析

有性杂交是目前大豆育种的主要途径,但大豆存在花器小,人工去雄授粉易伤柱头,伪杂种率高,杂交成活率低等不利因素。为了改进大豆杂交相关工作环节,提高杂交效率,采用整体去雄技术在大豆盛花期早晨的不同时间段去雄及早晨与下午去雄后用新鲜叶片包扎与不包扎处理,在不进行人工授粉前提下,根据结荚情况来分析伪杂种产生的主要原因。结果表明:在不同时间及不同包扎处理情况下,大豆去雄后不授粉均存在伪杂交现象,但去雄后包扎处理伪杂交较低;早晨去雄不授粉的时间越早伪杂交越低;不管包扎与否,下午去雄均比早晨去雄伪杂交低;去雄不授粉包扎处理时,不管下午还是早晨去雄,包扎均比不包扎伪杂交低。因此,黄淮海区域大豆早晨用整体去雄技术进行有性杂交是可行的,但去雄时间应在6∶30之前,并要以下午去雄为主,去雄后最好用新鲜叶片包扎处理。     

Regulating the content of white clover in mixed swards using grass-suppressing herbicides

A preliminary investigation evaluated six grass-suppressing herbicides applied on two occasions in late winter to a predominantly ryegrass ley containing only 15% ground cover of white clover. Substantial increases in clover growth, estimated visually, and flower head numbers per unit area were recorded in the first summer after treatment with 2·8 kg ha^-1 carbetamide, 0·8 kg ha^-1 propyzamide and 0·6 kg ha^-1 paraquat. To achieve these increases, visual estimates suggested that spring growth of grass was reduced by 40–80%. However, grass growth recovered fully by mid-summer on the majority of the treatments.
The following year five of the herbicides were compared in a field experiment. Dry matter (DM) and nitrogen (N) assessments of the grass and legume components were made at three harvests in the first growing season and a single harvest in the second year. Carbetamide, paraquat and, especially, propyzamide increased the proportion of clover in the DM (to 89% in the case of 1·2 kg ha^-1 propyzamide); in general, using herbicides to raise clover contents above 20% lead to reductions in spring grass growth of about 70%. However, such reduction was offset by subsequent increased growth so that total annual yields were largely unaffected. The increased legume content resulted in an increased N concentration in both grass and legume components, measured in the second summer. At this time, the greatest increase in total N yield (up to 35%) was recorded from 0·6 kg ha^-1 propyzamide. Potential uses to achieve legume dominance by grass-suppression are suggested and the needs for further research are outlined.     

Comparison of a straw/concentrate mixture with silage as a buffer feed for grazing dairy cows

Over a 24-week period during the 1986 summer, three groups of January- to March-calving dairy cows were either grazed conventionally (G) or grazed between morning and afternoon milkings and housed overnight and offered grass silage (Si) or a straw/concentrate mixture (St) ad libitum. The straw/concentrate mixture contained proportionately, 0-33 long barley straw, 0·28 ground barley, 0·12 soya bean meal, 0·25 molaferm and 0·22 minerals. The metabolizable energy (ME) and crude protein (CP) contents of the silage fed in weeks 1-8 and weeks 9-24 were 9-5 and 10⁻⁶ MJ kg MD⁻¹ and 160 and 191 g kg DM⁻¹ respectively. The straw mix had an ME content of 10⁻¹ MJ kg DM⁻¹ and CP content of 134 g kg DM⁻¹. Partial storage feeding with silage or a straw/concentrate mixture led to a decrease in estimated herbage DM intake. The feeding of the straw/concentrate mixture increased total DM intake, but the estimated total ME intake was similar for treatments G and St. The intakes (kg DM d⁻¹) for treatments G, Si and St were respectively, herbage 11·7,6·8,4·1; total l3·5,13·6,15·0; total ME intake (MJd⁻¹) 163, 155, 163.
Animal performance was, for treatments G, Si and St respectively: milk yield (kg d⁻¹) 19·2, 17·5, 19·1 (s.e.d. 0-87); milk fat content (g kg⁻¹) 36·9, 37·6, 37.1 (s.e.d. 1.22); milk protein content (g kg⁻¹) 35·3, 32·9, 33·4 (s.e.d. 0·76).     

Silage and milk production: a comparison between three grass silages of different digestibilities

Three first-harvest grass silages made from S23 perennial ryegrass cut on 25 May, 13 June and 25 June, and termed early, medium and late silages respectively, were compared in a 16-week feeding experiment with fourteen Ayrshire cows. The early, medium and late silages had D-values of 71·2, 65·0 and 62·5 respectively. The early silage was offered alone, whereas the medium and late silages were supplemented with a concentrate containing 208 g crude protein per kg DM at rates of 2, 3 and 4 kg per 10 kg milk. Silage DM intake was 12·8 kg per cow per d on the early silage treatment, and decreased progressively as concentrate intake increased on the other silage treatments. The mean daily milk yields were 16·0 kg per cow in the early silage treatment, 17·0, 18·4 and 20·4 kg per cow in the medium silage treatments, and 16·8, 18·1 and 20·2 kg per cow in the late silage treatments on the 2-, 3- and 4-kg concentrate treatments respectively. Fat concentration in the milk was not affected significantly by treatment, whereas the CP and SNF concentrations increased progressively and significantly as supplementary feeding increased. From the relationship between milk yield and concentrate intake it was calculated that the medium and late silages required a daily concentrate supplement of 2·1 kg DM per cow to give the same daily milk yields as the early silage.     

Drying and physical characteristics of matted ryegrass

Fresh ryegrass and lucerne were macerated and compressed into thin mats over a 4-week period at two yield levels. The mats were left lo dry outside during the day. and inside overnight, and compared with unconditioned crops. Under a low swath yield of 4 t DM ha⁻¹, mats required 0·7-1·4 mm pan evaporation lo reach 70% moisture, suitable for wilted silage, compared with 1·8-3·8 mm pan evaporation for unconditioned crops. On an average non-rainy day. mats were ready to harvest as wilted silage after 2–5 h, whereas the unconditioned crop required between 6 h and 36 h of wilting. With a high swath yield of 8 t DM ha⁻¹, mats required 1·4-3·0 mm pan evaporation to reach 70% moisture compared with 2·4-5·1 mm for unconditioned windrows. Low-yield mats reached 20% moisture, suitable for hay, in 2 d of drying, after 4·5-5·3 mm of pan evaporation. The thickness and cohesion of the mats were measured to assess their sensitivity to mechanical handling. The effect of controlled rainfall on mats was also investigated. Since mat making was most effective in low-yield crops, it could become a useful complement to low-input, extensive forage production. Mat making could eliminate most silage effluent losses; it could re introduce haymaking of ryegrass as a viable system under certain circumstances.     

The effect of a chemical hybridizing agent on the morphology and chemical composition of annual ryegrass

A chemical hybridizing agent (CHA: WL 84811, azetidine-carboxylic acid. Shell Chemical) was applied to annual ryegrass ( Lolium rigidum Gaud. cv. Wimmera) at four levels: 0, 1·6, 2·4 and 3·2 kg active ingredient (a.i.) ha⁻¹, at booting and at anthesis with a view to reducing seed set and increasing the proportion of cell solubles in the senescing and dead plants. The CHA treatments reduced stem elongation by up to 40% and reduced the proportion of pollinated florets progressively according to the concentration of CHA. At 3·2 kg (a.i.) ha⁻¹ only 14% of florets were fertilized compared with 86% on the zero (control) treatment.
The CHA treatments also resulted in progressive increases in the number of secondary tillers. Since there was no increase in the amount of neutral-detergent solubles associated with increased CHA application, it was concluded that storage carbohydrates, which might otherwise have been directed towards seed development, were partitioned towards increased tillering. There was no effect, therefore, on the proportion of neutral-detergent soluble material in senescing and dead plants.     

Effect of harvesting system on nutrient losses during silage making. 2. In-silo losses

Nutrient losses during the in-silo period were determined following the ensiling of grass using three differing harvesting systems. The treatments were either (a) harvesting herbage directly by means of a flail harvester (unwilted flail, UF) or (b) pre-mowing of herbage with a rotary drum mower and lifting using a precision chop forage harvester with wilting (WP) and without wilting (UP). Herbage was harvested and ensiled over the period 26–29 May 1982, and a good fermentation was achieved with all three silages. Losses of dry matter (DM), organic matter (OM) and crude protein (CP) during the in-silo period were greatest with the UF system, intermediate with the UP system and lowest with the WP system. Gross energy losses followed the same trend with losses (as a percentage of ensiled levels) of 18·6, 9·8 and 4·4% for the UF, UP and WP systems, respectively. Gross energy loss as effluent accounted for 24% and 22% of total gross energy ensiled for the UF and UP treatments. Patterns of effluent flow differed between the two unwilted silages with a higher peak flow rate with the UF material. Total effluent production at 354 ml (kg DM ensiled)^-1 was greater for the UF material than for the UP material (288 ml (kg DM ensiled)^-1). Nutrient loss through surface waste was similar for all three silages whereas gross energy losses arising through residual respiration, fermentation and gaseous loss amounted to 149%, 6·7% and 31% of that ensiled for the UF, UP and WP silages, respectively. The results of this study, taken in conjunction with those of an earlier study where field losses were assessed, indicate that recovery of gross energy during silage making was very similar for the UP and WP systems and only marginally greater than that recovered with the UF system.     

The effect of harvesting system on nutrient losses during silage making. 1. Field losses

An experiment was carried out during 1982 in which the effects of three differing harvesting systems on the field losses occurring during the conservation of grass as silage were examined. The treatments were either (a) harvesting herbage directly by means of a flail harvester (unwilted flail, UF), or (b) pre-cu ing of herbage with a rotary drum mower and lifting using a precision-chop forage harvester with wilting (WP) and without wilting (UP). Herbage was harvested on 26 May, 21 July and 7 September 1982. The mean yields of herbage produced from plots harvested by the three systems were not significantly different, with dry ma er (DM) yields of 12470, 12300 and 12230 kg ha^-1 for the UF, UP and WP treatments respectively. However, field losses with the UP and WP treatments were greater than with the UF treatment (P<0·01). As a result the yields of herbage ensiled with the UP and WP treatments, 11690 and 11320 kg DM ha^-1 respectively, were significantly lower than with the UF treatment, 12710 kg DM ha^-1 (P <0·05). Wilting of first cut herbage for a period of 72 h resulted in a significant reduction (P<0·05) in digestible organic ma er (DOM) concentration (determined in vitro), whereas small increases in DOM concentration were observed following wilting of second and third harvest material.
It is concluded that, even under favourable weather conditions, both pre-cutting and wilting of herbage prior to harvesting can result in considerable losses of nutrients in the field.     

Regrowth, morphology and persistence of Grasslands Puna chicory (Cichorium intybus L.) in response to grazing frequency and intensity

Effects of different grazing frequencies and intensities on herbage production (on both a unit pasture and individual plant basis) and on persistence of chicory ( Cichorium intybus L. cv. Grasslands Puna) were studied at Palmerston North, New Zealand (latitude 40°23'S) from November 1994 to November 1995. Three experiments were conducted on the same chicory stand, sown on 12 May 1994. The main grazing experiment had two grazing intensities, hard-lax grazing (50- to 100-mm stem stubble to mid-January, and thereafter 100- to 150-mm stem stubble) and lax grazing (100- to 150-mm stem stubble), and three grazing frequencies (1-, 2- or 4-week intervals). A subsidiary plant survival experiment compared the survival of 120 marked plants in ungrazed and grazed treatments. A late autumn grazing experiment examined the effects on plant persistence in the following spring. The greatest herbage mass (leaf + stem) resulted from the 4-week grazing frequency [9640 ± 874 kg dry matter (DM) ha⁻¹], in which stem mass was reasonably low (1270 ± 410 kg DM ha⁻¹), but was significantly higher in the 4-week grazing frequency than 1- and 2-week grazing frequencies ( P < 0·01). Grazing intensity had no significant effect except on the average stem mass of individual plants when the hard-lax intensity gave a lower stem mass ( P < 0·01). There were no interactions between grazing frequency and intensity in herbage mass. Plant density declined by 35% over the growing season with the decline unaffected by grazing intensity or frequency during the season. Grazing in late autumn resulted in approximately 27% less plants the following spring. It was concluded that grazing management through the growing season cannot be used to improve persistence without compromising leaf growth rate, but that avoidance of grazing late autumn will improve the persistence of chicory.     

THE CRUDE-PROTEIN CONTENT OF ITALIAN RYEGRASS

The effects of variety, date of cutting flush growth and level of nitrogenous manuring on the crude-protein content of Italian ryegrass were studied on plots cut five times in 1958.
Raising the level of nitrogenous fertilizer from 174 lb. to 348 1b. N/acre reduced the percentage of dry matter from 0·5 to 2·0 and increased the percentage of crude protein in the dry matter from 0·9 to 5·1, with net increases in crude protein/fresh material of from 0·06 to 0·57%. Varieties showed consistent differences, the three Westcrwolth types, New Zealand H.I and New Zealand Italian being higher in percentage crude protein than Ayrshire, Irish, Danish and S22.
Culling the flush growth earlier resulted in an increased percentage of crude protein for that particular cut, but no overall increase in mean crude-protein percentage.     

Influence of initial cutting date and cutting frequency on yield and quality of star, elephant and Guinea grasses

Two experiments were conducted to estimate the influence of initial cutting dates in March and April and of cutting frequencies on Guinea grass, elephant grass and star grass productivity. Increasing delay in date of first cut in the season resulted in a progressive increase in dry-matter yield. 72–81% of the tiller population at any cut were vegetative and this helped to maintain good aftermath yield for each date of first cut. Early April cuts gave the highest dry-matter yield and early March the least.
Harvesting frequencies affected the sward productivity such that the annual dry matter yield increased with increasing harvesting interval from 6800 kg ha^-1 for a 3-week interval to 13,000 kg ha^-1 for a 10-week interval. The proportion of green leaf in the dry matter dropped from 57·7% at 3-week intervals to 32·0% at 10-week intervals. Seasonal effects showed that potential yield was reduced by a short interval between harvests early in the season but not late in the season. Species differences in quality and in the harvesting interval that gave the maximum yields were noted. These results are discussed in relation to management of the sward throughout the growing season.     

Effects of application of nitrogen fertilizer on concentrations of P, K, S, Ca, Mg, Na, Cl, Mn, Fe, Cu and Zn in perennial ryegrass/white clover pastures in south-western Victoria, Australia

Effects of timing and rate of N fertilizer application on concentrations of P, K, S, Ca, Mg, Na, Cl, Mn, Fe, Cu and Zn in herbage from perennial ryegrass/white clover pastures were studied at two sites in south-western Victoria, Australia. Nitrogen fertilizer (0, 15, 25, 30, 45 and 60 kg ha^–1) was applied as urea in mid-April, early May, mid-May, early June and mid-June 1996 to pastures grazed by dairy cows. At Site 1, N fertilizer resulted in a linear increase in P, K, S, Mg and Cl concentrations in herbage and a linear decrease in Ca concentration. For all times of application, concentrations of P, K, Ca, Mg and Cl in herbage increased by 0·0048, 0·08, −0·010, 0·0013 and 0·053 g kg^–1 dry matter (DM) per kg N applied respectively. For S concentration, maximum responses occurred in mid-May (0·012 g kg^–1 DM per kg N applied). At Site 2, N fertilizer resulted in a linear increase in P, S and Na concentrations in herbage, a linear decrease in Ca concentration and a curvilinear increase in K and Cl concentration. The maximum responses for P, S and K concentrations in herbage occurred for the N application in mid-June and were 0·015, 0·008 and 0·47 g kg^–1 DM per kg N applied respectively. For Cl concentration, the maximum response occurred for the N application in early June and was 0·225 g kg^–1 DM per kg N applied. Overall, applications of N fertilizer up to 60 kg ha^–1 did not alter herbage mineral concentration to levels that might affect pasture growth or animal health.