Molecular Mapping of Three Loci Conferring Resistance to Maize (Zea mays L.) Rough Dwarf Disease

Maize rough dwarf disease (MRDD) is a viral disease that is widely distributed in the world and causes great losses in grain yield. During the past three decades, considerable progress has been made in the pathogen     

Identification of DNA Markers for Cotton Leaf Curl Disease (CLCD) in Cotton (Gossypium hirsutum L. )

Epidemic of cotton leaf curl virus disease(CLCD)was the compelling factor to devise newstrategies in cotton breeding programs ofPakistan.The evaluation of cotton genotypesagainst the CLCD resistance is difficult,expensive and time consuming in field andespecially in greenhouse due to unevendistribution of the disease.A marker-assisted     

Molecular Cloning and Characterization of Genes Involved in Cotton (Gossypium barbadense L.) Response to Verticillium dahliae

Verticillium dahliae Kleb.is a necrotrophic plant pathogen which causes serious soil borne vascular disease in cotton.The molecular basis the defense response of cotton to this pathogen is poorly understood.     

Molecular Marker-assisted Selection for Verticillium Wilt Resistance in Upland Cotton (Gossypium hirsutum)

Verticillium wilt is a global important disease ofcotton,which threatens the development ofcotton production seriously.Recent years,because of the change in climate and croppingpattern,Verticillium wilt was broke out incotton production areas in China,which became     

International Journal of Clinical Case Reports(IJCCR)

<正>International Journal of Clinical Case Reports(IJCCR,ISSN 1927-579X)is an international,open access,peer reviewed journal published online by BioP ublisher.The journal is considering all the latest and outstanding research articles,letters and reviews in all aspects of clinical case,containing clinical medicine which advance general medical knowledge;the event in the course of observing or treating a patient;new variations in disease     

Transcriptomic analysis reveals the defense mechanisms of citrus infested with Diaphorina citri

Huanglongbing(HLB) is a devastating disease that has led to an acute crisis for growers of citrus, one of the world’s most important fruit crops. The phloem-feeding Asian citrus psyllid(ACP), Diaphorina citri, is the main pest at the new shoot stage and is the only natural vector of HLB pathogenic bacteria. Little is known about how plants perceive and defend themselves from this destructive pest. Here, we characterized changes in the expression of various genes in citrus plants that were contin...     

ECG Information Management System of Virtual ECG Instrument

ECG is an important method in clinical heart disease diagnosis. The storing of lots of ECG and other corresponding materials in information management system has great value to medical researcher for their reference and research. Embedding the ECG information management system to virtual ECG instrument is not only enhanced the operating value but also increased the functions to virtual ECG instrument. The model of relation database and ActiveX Data Object (ADO) of VC++6.0 is used in the system. The key functions of Storing,Query,Deleting and core modules are introduced.     

International Journal of Clinical Case Reports(IJCCR)

正International Journal of Clinical Case Reports(IJCCR,ISSN 1927-579X)is an international,open access,peer reviewed journal published online by Bio Publisher.The journal is considering all the latest and outstanding research articles,letters and reviews in all aspects of clinical case,containing clinical medicine which advance general medical knowledge;the event in the course of observing or treating a patient;new variations in disease processes;as well as the expands the field of clinical relating to case reports.     

International Journal of Clinical Case Reports(IJCCR)

正International Journal of Clinical Case Reports(IJCCR,ISSN 1927-579X)is an international,open access,peer reviewed journal published online by Bio Publisher.The journal is considering all the latest and outstanding research articles,letters and reviews in all aspects of clinical case,containing clinical medicine which advance general medical knowledge;the event in the course of observing or treating a patient;new variations in disease processes;as well as the expands the field of clinical relating to case reports.     

Disease pattern and grade distribution of coast erosion in Liaodong bay

To identify distribution and development of coast erosion in Liaodong bay, erosion situations of typical coastal zone are studied on the basis of field investigation data. The results show that according to erosion landform features, coast erosion in Liaodong bay can be divided into five patterns: washing and abrasion type, wave collapse type, block avalanche type, karst depression type, interface slip type; according to degree of corrosion and siltation, coast erosion in Liaodong bay can be divided into five grades: serious erosion, intensive erosion, erosion, micro-erosion, stability. Sandy coast is eroded mainly in wave collapse type, rocky coast is eroded mainly in block avalanche type and karst depression type, and silty mud coast is eroded mainly in washing and abrasion type. Investigation on disease pattern and grade distribution of coast erosion is helpful to prevention of coastal zone in Liaodong bay.     

中国玉米抗病育种的若干问题及其解决途径

我国玉米生产中的主要病害有大斑病、小斑病、丝黑穗病、茎腐病和矮花叶病。研究结果表明:玉米对上述病害的抗性,主要表现为受多基因控制的水平抗性。不同玉米材料,抗病性有明显差别。F_1的抗病性,倾向抗病亲本或比亲本的平均病级低。实践证明,采用轮回选择法和利用多细胞质杂交种是行之有效的玉米抗病育种途径。     

玉米抗粗缩病自交系种质的发掘和遗传多样性及其在育种中的应用

玉米粗缩病是近十年来危害我国部分玉米产区的主要病害之一, 挖掘抗粗缩病玉米种质, 进而培育抗病品种是防治粗缩病危害最经济有效的途径。选用184份常用玉米自交系(包括111份普通玉米和73份糯玉米)于2009—2010年在玉米粗缩病重发区江苏沿江地区农业科学研究所试验田进行田间自然鉴定; 同时利用117对SSR引物对供试的玉米自交系进行多态性检测, 按UPGMA方法, 利用Powermarker 3.0软件对184份自交系进行系统聚类分析。筛选出2份高抗(T877和YJ7)、6份抗病普通自交系和2份糯玉米抗性种质。基于644个SSR多态性位点, 结合系谱资料和育种实践, 将184份自交系划为9个杂种优势亚群。自交系4S及其衍生系(第VI亚群)和来源于美国杂交种78599的玉米自交系(第VIII亚群)为玉米抗粗缩病育种的重要种质。     

玉米种质沈137抗病性及其利用潜力分析

在P群玉米种质沈137抗病鉴定结果的基础上,系统评价了沈137高抗弯孢叶斑病、灰斑病,兼抗大斑病、小斑病、南方锈病、穗腐病、茎腐病、丝黑穗病和矮花叶病等9种病害的抗病性及其抗病基因丰富而稳定的遗传特性。沈137是国内骨干自交系中抗病种类最多的自交系之一。充分利用沈137这类多抗资源及深入挖掘其应用潜力将成为应对玉米病害突发所带来生产隐患的重要途径。     

玉米矮花叶病毒抗性资源鉴定的研究

利用人工接毒方法对 70份玉米种质资源进行了两年玉米矮花叶病毒B株系的抗性鉴定。依据病情指数 ( % )将抗病程度分为高抗、抗、中抗及感病 4个等级。试验筛选出高抗自交系 4份、高抗单交种 3个、抗病毒自交系 10份、抗病单交种 3个 ;中抗自交系 6份、中抗群体 3个。讨论了这些种质资源在我国抗玉米矮花叶病遗传及育种研究上的应用价值     

基于Meta分析构建抗玉米粗缩病的染色体单片段代换系

为发掘玉米粗缩病抗性基因,解析其遗传规律。借助IBM2 2008 Neighbors遗传图谱,整合已报道的92个抗玉米粗缩病QTLs(Quantitative trait locus),通过Meta分析获得24个"一致性"抗病区间。利用生物信息分析,确定抗病区段的物理位置信息,在相关区段对郑58和齐319的全基因组重测序序列分析鉴定出7 142个InDel位点,其中546个含有InDel位点的序列可用于引物开发,进一步通过试验筛选出在郑58和齐319第2,4,5,6,7,8,10号染色体上的多态InDel位点158个。以抗粗缩病玉米自交系齐319为供体亲本,感病优良自交系郑58为受体亲本,利用上述InDel标记辅助选择构建整套染色体单片段代换系材料。为抗玉米粗缩病QTL位点的精细定位提供了可能,也为抗病育种提供新的种质资源。     

玉米粗缩病病情严重度分级标准及病情指数与产量损失率关系的研究

通过对农大108、郑单958和德玉18等3个玉米品种感染玉米粗缩病后株高与单株产量的测定,按病株相对健株株高的比值推导出了玉米粗缩病严重度分级标准,即病株相对健株株高的比依次为1,4/5,2/3,1/2,1/3,其对应病株的病级分别是0,1,2,3,4级;明确了0~4级玉米粗缩病病情严重度分级标准与产量损失的关系,即0,1,2,3,4级病株,产量损失率依次为0,25%,50%,75%和100%。利用该分级标准对10个生产品种的玉米粗缩病病情进行调查,构建了回归方程Y=-0.253 1.02X。利用16个生产品种对方程进行检验,检验准确率为:理论产量损失率与实际产量损失率没有显著差异。应用此方程,可预测玉米粗缩病导致的产量损失。     

玉米自交系对大斑病菌的抗性鉴定及抗性来源分析

玉米大斑病是一种严重影响玉米产量的病害,在全国各地多有发生。通过筛选抗病玉米自交系,选育优良抗病品种可以有效防止大斑病的发生。试验于2017-2018年,用田间人工接种大斑病混合菌的方法,对185份玉米种质进行了玉米大斑病抗性鉴定与评价,筛选出高抗自交系16份,其中自选系7份,并对玉米自选系抗病性来源进行分析,发现Y6和J1577等品系抗病性强且可以稳定遗传。     

玉米矮花叶病的抗性遗传分析

以玉米自交系X178和B73杂交培育的183个重组自交系为试验材料,采用人工接种的方法,在苗期、拔节期、抽雄期和成株期进行了玉米矮花叶病的抗性鉴定.结果表明,183份重组自交系之间对玉米矮花叶病的抗性存在着较大的差异,在抽雄期和成株期各有3个高抗的超亲分离;苗期和拔节期的病株率均呈现正态分布,抽雄期和成株期则表现为感病家系较多的偏态分布,且家系病株率的变异系数随生长发育变得越来越小,但抗性遗传力则越来越大.说明对玉米矮花叶病的抗性鉴定在成株期较准,苗期受环境影响较大;成株期的抗性由2～3对主基因控制,同时存在多基因修饰或互作;抗性基因之间以加性效应为主.     

国家玉米审定品种数据库的建设研究

简要介绍了国内外品种管理信息数字化的发展概况和我国玉米审定品种数据信息的管理现状,阐述了利用Visual Basic6.0和SQL Server2000软件构建国家玉米审定品种数据库的技术方法,初步建成了内容涵盖农艺性状、产量、品质、抗病虫性、全国播种面积变化趋势等相关信息,同时具备快速检索和应用功能的数据库,为我国玉米育种及新品种的管理、推广提供了科学参考。     

玉米多胞质雄性不育系CF5-11111的选育及抗病研究

以雄性不育细胞质（CMS）中s群的s，M，R与21A及c群的Rb，Es等多种细胞质为背景采用回交转育的方式，完成了CF5-11111雄性不育系的转育，不育性稳定。根据21A，s，M,Rb,Es胞质不育系具有较稳定的不育性和5种胞质抗病性的有关研究，研究认为21A，s，M，Rb。Es型雄性不育细胞质完全可以在生产上应用。