Soil microbial biomass response to woody plant invasion of grassland

Woody plant proliferation in grasslands and savannas has been documented worldwide in recent history. To better understand the consequences of this vegetation change for the C-cycle, we measured soil microbial biomass carbon (C_mic) in remnant grasslands (time 0) and woody plant stands ranging in age from 10 to 130 years in a subtropical ecosystem undergoing succession from grassland to woodlands dominated by N-fixing trees. We also determined the ratio of SMB-C to soil organic carbon (C_mic/C_org) as an indicator of soil organic matter quality or availability, and the metabolic quotient (qCO₂) as a measure of microbial efficiency. Soil organic carbon (C_org) and soil total nitrogen (STN) increased up to 200% in the 0–15 cm depth increment following woody plant invasion of grassland, but changed little at 15–30 cm. C_mic at 0–15 cm increased linearly with time following woody plant encroachment and ranged from 400 mg C kg⁻¹ soil in remnant grasslands up to 600–1000 mg C kg⁻¹ soil in older (>60 years) woody plant stands. C_mic at 15–30 cm also increased linearly with time, ranging from 100 mg C kg⁻¹ soil in remnant grasslands to 400–700 mg C kg⁻¹ soil in older wooded areas. These changes in C_mic in wooded areas were correlated with concurrent changes in stores of C and N in soils, roots, and litter. The C_mic/C_org ratio at 0–15 cm decreased with increasing woody plant stand age from 6% in grasslands to <4% in older woodlands suggesting that woody litter may be less suitable as a microbial substrate compared with grassland litter. In addition, higher qCO₂ values in woodlands (0.8 mg CO₂-C g⁻¹ C_mic h⁻¹) relative to remnant grasslands (0.4 mg CO₂-C g⁻¹ C_mic h⁻¹) indicated that more respiration was required per unit of C_mic in wooded areas than in grasslands. Observed increases in C_org and STN following woody plant encroachment in this ecosystem may be a function of both greater inputs of poor quality C that is relatively resistant to decay, and the decreased ability of soil microbes to decompose this organic matter. We suggest that increases in the size and activity of C_mic following woody plant encroachment may result in: (a) alterations in competitive interactions and successional processes due to changes in nutrient dynamics, (b) enhanced formation and maintenance of soil physical structures that promote C_org sequestration, and/or (c) increased trace gas fluxes that have the potential to influence atmospheric chemistry and the climate system at regional to global scales.     

Depth distribution of soil organic C and N after long-term soybean cropping in Texas

Crop management practices have potential to enhance subsoil C and N sequestration in the southern U.S., but effects may vary with tillage regime and cropping sequence. The objective of this study was to determine the impacts of tillage and soybean cropping sequence on the depth distribution of soil organic C (SOC), dissolved organic C (DOC), and total N after 20 years of treatment imposition for a silty clay loam soil in central Texas. A continuous soybean monoculture, a wheat–soybean doublecrop, and a sorghum–wheat–soybean rotation were established under both conventional (CT) and no tillage (NT). Soil was sampled after soybean harvest and sectioned into 0–5, 5–15, 15–30, 30–55, 55–80, and 80–105 cm depth intervals. Both tillage and cropping intensity influenced C and N dynamics in surface and subsurface soils. No tillage increased SOC, DOC, and total N compared to CT to a 30 cm depth for continuous soybean, but to 55 cm depths for the more intensive sorghum–wheat–soybean rotation and wheat–soybean doublecrop. Averaged from 0 to 105 cm, NT increased SOC, DOC, and total N by 32, 22, and 34%, respectively, compared to CT. Intensive cropping increased SOC and total N at depths to 55 cm compared to continuous soybean, regardless of tillage regime. Continuous soybean had significantly lower SOC (5.3 g kg⁻¹) than sorghum–wheat–soybean (6.4 g kg⁻¹) and wheat–soybean (6.1 g kg⁻¹), and 19% lower total N than other cropping sequences. Dissolved organic C was also significantly higher for sorghum–wheat–soybean (139 mg C kg⁻¹) than wheat–soybean (92 mg C kg⁻¹) and continuous soybean (100 mg C kg⁻¹). The depth distribution of SOC, DOC, and total N indicated treatment effects below the maximum tillage depth (25 cm), suggesting that roots, or translocation of dissolved organic matter from surface soils, contributed to higher soil organic matter levels under NT than CT in subsurface soils. High-intensity cropping sequences, coupled with NT, resulted in the highest soil organic matter levels, demonstrating potential for C and N sequestration for subsurface soils in the southern U.S.     

Soil fertility properties on Agave angustifolia Haw. plantations

This study examined the variations in soil physical, chemical and biological properties from Agave angustifolia fields in three sites with different topographic conditions (valley, hill and mountain), in Oaxaca, Mexico, associated with the tillage systems, disk ploughing (DP), animal drawn ploughing (ADP) and minimum tillage (MT), respectively. Plant ages were 1.5–3.5 years (class 1), 3.6–5.5 years (class 2) and 5.6–7.5 years (class 3). Soil samples were taken at two soil depths (0–20 and 21–40 cm) from plots of 4000 m² within each site and plant age classes, during the spring of 2005. The main changes in soil properties were found in the mountain site. Soil bulk density (2.0 g cm⁻³), cone penetration resistance (CPR) (3.96 MPa), 0.7 and 1.0 mm water stable aggregates (WSA) (28.3 g kg⁻¹ and 102.2 g kg⁻¹, respectively) were higher in the mountain site than in the hill and valley fields. This result is consistent with the rocky substrate beneath the shallow soil. Soil organic carbon (SOC) (23.9 g kg⁻¹), available N (23.1 mg kg⁻¹) and soil microbial biomass carbon (SMBC) (969.6 μg g⁻¹) at the mountain site showed the highest values, suggesting that MT practiced in this topographic condition favours the organic matter accumulation and biological activity. Soil microbial biomass carbon and SOC seem to be the soil properties that were mainly affected by the sites and soil management associated with them. For the three sites, SOC, P_Olsen, available N, exchangeable Na⁺ and SMBC were higher at 0–20 cm depth than at 21–40 cm depth within each site. Exchangeable Ca²⁺ and K⁺, P_Olsen and CPR increased with plant age. In contrast, available N decreased. Soil chemical properties were more affected by the age of the plant than physical and biological properties. Results reported here represent a reference of the fertility properties of soils cultivated with A. angustifolia, which could be used in further studies focused on management and tillage systems.     

Soil micronutrient availability to crops as affected by long-term inorganic and organic fertilizer applications

Micronutrient status in soils and crops can be affected by different fertilization practices during a long-term field experiment. This paper investigated the effects of different fertilization treatments on total and DTPA-extractable micronutrients in soils and micronutrients in crops after 16 year fertilization experiments in Fengqiu County, Henan Province, China. The treatments of the long-term experiment included combinations of various rates of N, P and K in addition to two rates of organic fertilizer (OF) treatments. Winter wheat and summer maize were planted annually. Soil macro- and micronutrients along with pH and organic matter (OM) were analyzed. Grains and above ground parts of both crops in the final year were harvested and analyzed for Cu, Zn, Fe and Mn. The results showed that soil Cu, Zn, Fe and Mn concentrations did not change among the different treatments to a significant level, except for a slight decrease of soil Zn in the CK (no fertilizer application) compared to the OF treatment. The DTPA-extractable soil Zn, Fe and Mn concentrations increased from 0.41 to 1.08 mg kg⁻¹, from 10.3 to 17.7 mg kg⁻¹, and from 9.7 to 11.8 mg kg⁻¹, respectively, with increasing soil OM content, thus showing the importance of soil OM in micronutrient availability for crops. The NPK treatment also had higher DTPA-extractable micronutrient concentrations in soil. Deficiency of N or P resulted in a low yield but high micronutrient concentrations in crops except Cu in maize stalks. Higher available soil P significantly decreased crop micronutrients, possibly because of their precipitation as metal phosphates. Maize stalks contained higher concentrations of micronutrients than those of wheat straw, whereas wheat grain had higher micronutrients than those of corn grain. The transfer coefficients (TCs) of micronutrients from straw to grain were significantly different between winter wheat (1.63–2.52 for Cu; 2.31–3.82 for Zn; no change for Fe; 0.55–0.84 for Mn) and summer maize (0.24–0.50 for Cu; 0.50–1.21 for Zn; 0.02–0.04 for Fe; 0.07–0.10 for Mn). In conclusion, application of organic matter significantly increased the DTPA-extractable concentrations of Zn, Fe and Mn compared to the CK, grain and vegetative tissue in the CK and NK had higher micronutrient concentrations than those in other treatments.     

Effects of disturbance and glucose addition on nitrous oxide and carbon dioxide emissions from a paddy soil

The effects of disturbance and glucose addition on N₂O and CO₂ emissions from a paddy soil at 45% WFPS (water-filled pore space) and at 25 °C were determined. During a 45-day incubation, disturbances with and without glucose addition were imposed 0, 1, 3, and 5 times. The total amount of glucose added to soil with 1, 3, and 5 disturbances was equal (0.6% of oven-dry soil basis). Strong nitrification occurred in the paddy soil during the incubation. Disturbance alone did not influence N₂O and CO₂ emissions significantly, but disturbance with glucose addition did (P < 0.01). A flush of N₂O as well as CO₂ was always observed following disturbance with glucose addition. The discrepancy in N₂O emission between disturbance alone and disturbance with glucose addition was ascribed to the different magnitude of denitrification and/or heterotrophic nitrification. Greater cumulative emission of N₂O was observed in the treatment of three disturbance times with glucose addition (4.3 mg N kg⁻¹ soil), compared with five disturbances with glucose addition (2.5 mg N kg⁻¹ soil) and one disturbance with glucose addition (2.5 mg N kg⁻¹ soil). Cumulative CO₂ emission was significant larger in one and three disturbances with glucose addition than that five disturbance with glucose addition. Supplies of available organic C appear to be a critical factor controlling denitrification and/or heterotrophic nitrification processes and N₂O emission under relatively low moisture conditions, i.e. 45% WFPS.     

Effects of mechanical energy inputs on soil respiration at the aggregate and field scales

Cultivation machinery applies large amounts of mechanical energy to the soil and often brings about a decrease in soil organic carbon (SOC). New experiments on the effects of mechanical energy inputs on soil respiration are reported and the results discussed. In the laboratory, a specific energy, K, of 150 J kg⁻¹, similar to that experienced during typical cultivation operations, was applied to soil aggregates using a falling weight. Respiration (carbon dioxide, CO₂ emission) of the samples was then measured by an electrical conductimetric method. Basal respiration (when K=0) measured on Chromic Luvisol aggregates, was found to increase with increasing SOC, from 1.88 μg CO₂ g⁻¹ h⁻¹ for a permanent fallow soil (SOC=11 g kg⁻¹) to 8.25 μg CO₂ g⁻¹ h⁻¹ for a permanent grassland soil (SOC=32 g kg⁻¹). Basal respiration of a Calcic Cambisol, more than doubled (2.0–5.2 μg CO₂ g⁻¹ h⁻¹) with increasing gravimetric soil water contents. Mechanical energy inputs caused an initial burst of increased respiration, which lasted up to 4 h. Over the following 4–24 h period, arable soils with lower SOC contents, (11–21 g kg⁻¹), respiration rates dropped back to a level, approximately 1.14 times higher than the basal value. However, grassland soils with higher SOC contents (28–32 g kg⁻¹), increases in this longer-term respiration rate following 150 J kg⁻¹ of energy, were negligible. A field experiment, in which CO₂ was measured by infra-red absorption, also showed that tillage stimulated increased levels of soil respiration for periods ranging from 12 h to more than one week. The highest respiration rates, 80 mg CO₂ m⁻² h⁻¹ were associated with high energy, powered tillage on clay soils. On the same soil, low energy draught tillage resulted in a respiration rate of approximately half this value. The results of these experiments are discussed in relation to equilibrium levels of soil organic matter. The application of known quantities of mechanical energy to soil aggregates under laboratory conditions, in order to simulate the effect of different cultivation practices, when combined with the subsequent measurement of soil respiration, can provide useful indication of the likely consequences of soil management on SOC.     

The effect of seeding and tillage methods on productivity of rice–wheat cropping system

Many farmers in southeast Asia are growing rice on unpuddled soil. This practice does not permit breaking of the deadlock of increase in productivity in spite of using high yielding varieties and practising all known scientific technologies. Furthermore, farmers do dry seeding which leads to heavy infestation of weeds and reduces response to other inputs. Similarly, in rice–wheat belt due to short turn around time farmers resort to broadcast sowing of wheat after rice and no data on benefits or otherwise of tillage are available. A field study was therefore conducted for 3 years (1993–1994 to 1995–1996) at the Indian Agricultural Research Institute, New Delhi to study the effect of tillage and seeding methods in rice–wheat cropping system. Treatments included four combinations of two puddling treatments (puddling and no puddling) and two methods of rice seeding (direct seeding and transplanting) in rice and two tillage treatments (zero and conventional tillage) in wheat. Results indicated that puddling increased grain yield of rice by 0.7–1 t ha⁻¹ and of succeeding wheat by 0.2–0.4 t ha⁻¹, straw yield of rice by 0.8–1.7 t ha⁻¹ and of succeeding wheat by 0.1–1.0 t ha⁻¹.

Puddling reduced water requirement of rice by 75 mm ha and increased net return of rice–wheat system by US $175 ha⁻¹. Transplanted rice gave significantly higher grain and straw yields and net returns than direct seeded rice both on puddled and unpuddled seedbed. Conventional tillage in wheat also increased productivity of rice–wheat cropping system significantly over zero tillage after both puddled and non-puddled rice. Our results thus show that rice should be grown on puddled soil and wheat after rice should be sown after conventional tillage.     

Long-term manuring and fertilization effects on soil organic carbon pools in a Typic Haplustept of semi-arid sub-tropical India

Soil is a potential C sink and could offset rising atmospheric CO₂. The capacity of soils to store and sequester C will depend on the rate of C inputs from plant productivity relative to C exports controlled by microbial decomposition. Management practices, such as no-tillage and high intensity cropping sequences, have the potential to enhance C and N sequestration in agricultural soils. An investigation was carried out to study the influence of long-term applications of fertilizers and manures on different organic C fractions in a Typic Haplustept under intensive sequence of cropping with maize–wheat–cowpea in a semi-arid sub-tropic of India. In 0–15 cm, the bulk density was lowest (1.52 Mg m⁻³) in plots treated with 100% NPK + FYM, while the control treatment showed the highest value (1.67 Mg m⁻³). Balanced application of NPK (100% NPK) showed significantly lower bulk density (1.56 Mg m⁻³) over either 100% N (1.67 Mg m⁻³) or 100% NP (1.61 Mg m⁻³) in surface soils. The application of super-optimal dose of NPK (150% NPK) showed higher total organic C (TOC) (12.9 g C kg⁻¹) over either 50% NPK (9.3 g C kg⁻¹) or 100% NPK (10.0 g C kg⁻¹) in 0–15 cm soil layer. There was an improvement in TOC in 100% NPK or 100% NP (9.3 g C kg⁻¹) over 100% N (8.7 g C kg⁻¹) in the same depth. The application of FYM with 100% NPK showed 15.2, 9.9 and 5.2 g C kg⁻¹ in 0–15, 15–30 and 30–45 cm, respectively. Application of graded doses of NPK from 50 to 150% of recommendation NPK significantly enhanced other organic C fractions like, microbial biomass C (MBC), particulate organic C (POC) and KMnO₄ oxidizable C (KMnO₄–C) in all the three soil depths. The TOC in 0–45 cm soil depth in 150% NPK (63.5 Mg C ha⁻¹) was increased by 39% over that in 50% NPK treatment (51.5 Mg C ha⁻¹) and 29% over that in 100% NPK treatment (54.1 Mg C ha⁻¹). Integrated use of farmyard manure with 100% NPK (100% NPK + FYM) emerged as the most efficient management system in accumulating largest amount of organic C (72.1 Mg C ha⁻¹) in soil. Nevertheless, this treatment also sequestered highest amount of organic C (731 kg C ha⁻¹ year⁻¹). Particulate organic carbon, a physically protected carbon pool in soil, could well be protected in sub-surface soil layers than in surface soil layer as a means of carbon aggradations. Microbial metabolic quotient (qCO₂) was significantly lower in 100% NPK + FYM over other treatments to indicate this to be the most efficient manuring practice to preserve organic carbon in soil where it facilitates aggradations of more recalcitrant organic C in soil. As compared to POC, total TOC proved to be a better predictor of MBC as it strongly correlated with total carbon mineralized from soil.     

Erosional effects on soil organic carbon stock in an on-farm study on Alfisols in west central Ohio

Soil erosion and depositional processes in relation to land use and soil management need to be quantified to better understand the soil organic carbon (SOC) dynamics. This study was undertaken on a Miamian soil (Oxyaquic Hapludalfs) under on-farm conditions in western Ohio with the objectives of evaluating the effects of degree of erosion on SOC stock under a range of tillage systems. Six farms selected for this study were under: no-till (NT) for 15, 10, 6 and 1.5 years; chisel till every alternate year with annual manure application (MCT); and annual chisel till (ACT). A nearby forest (F) site on the same soil was chosen as control. Using the depth of A horizon as an indicator of the degree of erosion, four erosion phases identified were: uneroded (flat fields under F, NT15, and on the summit of sloping fields under NT10, NT6, NT1.5 and MCT); deposition (NT10, NT6, NT1.5 and ACT); slight (NT10, MCT and ACT); and moderate erosion (NT10 and ACT). Core and bulk soil samples were collected in triplicate from four depths (i.e., 0–10, 10–20, 20–30 and 30–50 cm) for each erosional phase in each field for the determination of bulk density, and SOC concentrations and stocks. SOC concentration in NT fields increased at a rate of 5% year⁻¹ for 0–10 cm and 2.5% year⁻¹ for 10–20 cm layer with increasing duration under NT. High SOC concentration for NT15 is indicative of SOC-sequestration potential upon conversion from plow till to NT. SOC concentration declined by 19.0–14.5 g kg⁻¹ in MCT and 11.3–9.7 g kg⁻¹ in NT10 between uneroded and slight erosion, and 12.0–11.2 g kg⁻¹ between slight and moderate erosion in ACT. Overall SOC stock was greatest in the forest for each of the four depths. Total SOC stock for the 50 cm soil layer varied in the order F (71.99 Mg ha⁻¹) > NT15 (56.10 Mg ha⁻¹) > NT10 (37.89 Mg ha⁻¹) = NT6 (36.58 Mg ha⁻¹) for uneroded phase (P < 0.05). The lack of uneroded phase in ACT indicated high erosion risks of tillage, as also indicated by the high SOC stock for deposition phase from 0 to 50 cm soil layer (ACT (56.56 Mg ha⁻¹) > NT1.5 (42.70 Mg ha⁻¹) > NT10 (30.97 Mg ha⁻¹)). Tillage increased soil erosion and decreased SOC stock for top 10 cm layer for all erosional phases except deposition.     

Response of soil physical properties to tillage and residue management on two soils in a cool temperate environment

In view of their potential benefits, reduced or no tillage (NT) systems are being advocated worldwide. Concerns about impairment of some soil conditions, however, cast doubt on their unqualified acceptance. We evaluated the effects of 6 years of tillage and residue management on bulk density, penetration resistance, aggregation and infiltration rate of a Black Chernozem at Innisfail (loam, 65 g kg⁻¹ organic matter, Udic Boroll) and a Gray Luvisol at Rimbey (loam, 31 g kg⁻¹ organic matter, Boralf) cropped to monoculture spring barley (Hordeum vulgare L.) in a cool temperate climate in Alberta, Canada. Tillage systems were no tillage and tillage with rototilling (T), and two residue levels were straw removed (−S) and straw retained (+S). Bulk density (BD) of the 0–7.5 and 7.5–15 cm depths was significantly greater under NT (1.13–1.58 Mg m⁻³) than under T (0.99–1.41 Mg m⁻³) in both soils, irrespective of residue management. In both soils, penetration resistance (PR) was greater under NT than under T to 15 cm depth. Residue retention significantly reduced PR of the 0–10 cm soil in NT, but not in T. In the 0–5 cm depth of the Black Chernozem, the >2 mm fraction of dry aggregates was highest under NT + S (72%), and lowest under T − S (50%). The wind-erodible fraction (dry aggregates <1 mm size) was smallest (18%) under NT + S and largest (39%) under T − S. Soil aggregation benefited more from NT than from residue retention. Proportion of wind-erodible aggregates was generally greater in the Gray Luvisol than in the Black Chernozem. In the Black Chernozem, steady-state infiltration rate (IR) was significantly lower (33%) under NT than under T. Residue retention improved IR in both NT and T. In the Gray Luvisol, IR was not significantly affected by tillage and residue management. Despite firmer soil, NT and residue retention are recommended to improve aggregation in the cool temperate region of Western Canada.     

Soil C, N and crop yields in Alabama's long-term ‘Old Rotation' cotton experiment

The Old Rotation cotton experiment at Auburn, Alabama, is the oldest, continuous cotton experiment in the world (cf. 1896). Long-term cropping systems provide a unique opportunity to observe the effects of 100 years of cropping on soil organic carbon (SOC). The objective of this paper was to summarize limited data on SOC and N cycling in this historic experiment. Soil organic C has been measured on the 13 plots (6 cropping systems) in 1988, 1992 and 1994. Long-term planting of winter legumes with no other source of N applied resulted in higher SOC (9.5 g C kg⁻¹) in the plow layer (0–20 cm depth) compared to continuous cotton with no winter cover crops (4.2 g C kg⁻¹). A 3-year rotation of cotton–winter legumes–corn–small grain–soybean resulted in 12.1 g C kg⁻¹. There was a significant (P<0.05), quadratic cotton yield response (R²=0.54) to increasing SOC. Winter legume cover crops supplied between 90 and 170 kg N ha⁻¹. Where no N has been applied in fertilizer or from a legume crop, annual N removal in the cotton crop is around 13 kg ha⁻¹, about the same as that fixed in precipitation.     

Soil organic matter and related physical properties in a Mediterranean wheat-based rotation trial

Under semi-arid Mediterranean conditions, limited moisture is the main constraint to rainfed cropping with wheat (Triticum aestivum), barley (Hordeum vulgare), and food and forage legumes. With increasing land-use pressure, moisture-conserving fallowing is being replaced by continuous cropping, which is considered an unsustainable practice. Thus, a long-term trial with durum wheat (T. turgidum var. durum) was established in 1983 at Tel Hadya, Aleppo, Syria (mean annual rainfall 330 mm) to assess alternative rotation options to fallow and continuous cropping. Nitrogen (N) and grazing/residue management were secondary factors. Soil aggregation, infiltration, hydraulic conductivity, and total soil organic matter and component fractions (fulvic and humic acids and polysaccharides) were determined at the end of 12 years. Some rotations, e.g., medic (Medicago sativa) and vetch (Vicia faba), significantly increased soil organic matter (12.5–13.8 g kg⁻¹ versus 10.9–11 g kg⁻¹ for continuous wheat and wheat/fallow). All measurements, or indices, indicated parallel trends with increasing organic matter, e.g., coefficients of macro-structure, micro-aggregation, and water-stable aggregates, and decreasing dispersion. Similarly, legume rotations had higher infiltration rates (16.2–21.8 cm h⁻¹ versus 13.9–14.4 cm h⁻¹ with continuous wheat and wheat/fallow) and hydraulic conductivity rates (8.7–12.4 cm h⁻¹ versus 6.2–7.4 cm h⁻¹ with continuous wheat and wheat/fallow). We conclude that cereal/legume rotations, in addition to being biologically and economically attractive, also enhance soil quality and thus promote soil use sustainability in fragile semi-arid areas as in the Mediterranean zone.     

Role of nutrient amendments in the success of half-moon soil and water conservation practice in semiarid Burkina Faso

The half-moon technique has been recently introduced in northern Burkina Faso as a method for the rehabilitation of sealed and crusted bare soils locally called zipellé. As this technique, like zaï and mulching practices, interested many farmers, a trial was conducted to study the effect on soil productivity of half-moon technique in association with different sources of nutrients. The experimental design consisted of treatments in which the half-moon was combined with organic or mineral fertilisers. The soil was a Ferric Lixisol with a rooting depth of 30 cm, low contents of organic matter (12 g kg⁻¹), nitrogen (0.6 g kg⁻¹) and available phosphorus (6.6 mg kg⁻¹). Applying compost or animal manure allowed yields from 900 to 1600 kg ha⁻¹ of sorghum grain, i.e. 20–39 times the yield obtained in the half-moon treatment without any amendment. Combining local rock phosphate to compost in the half-moon basins increased sorghum grain yield by 10% the first year and 26% the second year. This study showed that restoring favourable soil moisture conditions by breaking up the surface crust to improve water infiltration was not enough to improve sorghum production on the degraded zipellé. Removal of the water constraint by destroying the surface hard pan revealed the second major constraint, i.e. soil acidity and nutrient deficiency. Well-decomposed organic matter such as animal manure and compost supplied in the half-moons were good substrates that provided sorghum with the nutrients required for growth. Moreover, adding local rock phosphate to compost appeared to be an alternative for improving soil productivity. It is concluded that in the Sahelian zone, half-moon technique with appropriate nutrient management could be an effective method for the rehabilitation of degraded soil productivity.     

Toxicity of chemical warfare agent HD (mustard) to the soil microinvertebrate community in natural soils with contrasting properties

A microcosm technique was used to determine the ecotoxicity of the chemical warfare agent HD (mustard) to the indigenous soil microinvertebrate communities. HD was thoroughly incorporated into Sassafras sandy loam (SSL) soil (4.9% OM) and an oak-beech forest silt loam soil (FS, 16% OM) at nominal HD concentrations ranging from 6 to 1076 mg kg⁻¹. After a 7-day incubation period, microarthropods were extracted from soils using a high-gradient extractor and sorted to Acari suborders Prostigmata, Mesostigmata, and Oribatida, and the insect order Collembola. Nematodes were extracted using Baermann funnels and were sorted into bacterivore, herbivore, fungivore and omnivore/predator trophic groups. Microarthropods were more sensitive to HD in both soil types compared with nematodes. The EC₅₀ values for total numbers of microarthropods in SSL and FS were similar (65 and 71 mg kg⁻¹, respectively). The EC₅₀ values for total numbers of nematodes were 130 and 235 mg kg⁻¹, respectively. Toxicity of HD to nematodes was significantly greater in SSL soil compared to FS, based on 95% confidence intervals. Results show that community-level assessment of chemical toxicity in soil using a microcosm assay is sufficiently robust and can provide the means for validating the ecotoxicity data from standardized laboratory single-species toxicity tests.     

Rotation and tillage effects on soil organic carbon sequestration in a typic Hapludalf in Southern Ontario

Increased use of conservation tillage is being considered as a way to sequester atmospheric C in the soil. However, little information exists on the effect of rotation and its interaction with tillage on soil organic carbon (SOC). A research trial with combinations of rotations and tillage treatments was sampled 20 years after its establishment to assess the effects on SOC sequestration in a typic Hapludalf in southern Ontario, Canada. The cropping treatments included continuous corn (zea mays L.), six rotations comprised of 2 years of corn following 2 years of another crop or crop sequence, and continuous alfalfa (Medicago sativa L.). Each rotation was split into either fall moldboard plow (MP) or fall chisel plow (CP) treatments. Continuous alfalfa was plowed and replanted every 4 years. Soil samples were taken incrementally to a depth of 40 cm and SOC and bulk density determined. The average SOC concentration (0–40 cm) was greatest in continuous alfalfa (18.0 g C kg⁻¹). The treatments of soybean (Glycine max L.Merr.)+winterwheat (Triticum aestivum L.) or barley+barley (Trifolium pratense L.) (interseeded with red clover) followed by 2 years of corn had higher SOC concentrations (17.2–17.3 g C kg⁻¹) than continuous corn and the treatments of 2 years of corn following 2 years of alfalfa or soybean (16.4–16.5 g C kg⁻¹). The rotation of 2 years of barley followed by 2 years of corn had the lowest SOC concentrations (15.2 g C kg⁻¹). On an equivalent mass basis, the rotations of soybean+winterwheat or barley+barley (underseeded with red clover) followed by 2 years of corn, had 2–9 Mg ha⁻¹ more C than the other corn-based rotations. Including red clover in the winter wheat seemed to accelerate the rate of C mineralization compared to winter wheat without red clover; whereas interseeding red clover with barley increased SOC contents compared to excluding red clover in the barley rotation. More SOC was found in the top 10 cm and less in the 10–20 cm depth of the CP than in the MP soils. However, the CP did not increase the SOC content (0–20 cm) above that of MP indicating that this form of reduced tillage did not increase C sequestration in any of the rotations on this soil.     

Seasonal dynamics of soil microbial biomass in coastal sand dune forest

Sand dunes are a typical landscape in the coast of western Taiwan, where Casuarina forests were established decades ago to stabilize sand dunes and protect the inland vegetation. Study of microbial biomass in such an ecosystem may give insights into the role of microbes in soil fertility and nutrient cycling. We established our study sites in two topographic units based on elevation and drainage types: upland and lowland. The study lasted for 2 years, and soil samples were collected every 3 months. Microbial biomass C (C_mic) and N (N_mic) were high in a shallow humic layer that rested on top of the soil (1222–1319 mg kg⁻¹ for C_mic and 245–276 mg kg⁻¹ for N_mic) and declined sharply to only one-tenth of the above values in the underlying surface soil (0–10 cm depth). Microbial biomass C_mic and N_mic in humic and surface soil were not significantly different between upland and lowland sites. In the upland soils, the mean C_mic was highest in autumn for both the humic and surface soil, and lowest in spring and summer for the humic layer and summer for the surface soil layer. In the lowland soils, the C_mic was highest in winter for both humic and surface soil, and lowest in spring and autumn for the humic layer and spring and summer for surface soil. Strong fluctuations of C_mic and N_mic were associated with the soil moisture prior to sampling, which appeared to control the size of microbial biomass in this environment. Temperature had little effect on the dynamics of soil microbial biomass in the sand dune forest ecosystem.     

Physical characterization of a soil amended with organic residues in a rice–wheat cropping system using a single value soil physical index

The effect of soil incorporations of lantana (Lantana spp.) biomass, an obnoxious weed, on physical environment of a silty clay loam soil (Typic Hapludalf) under rice (Oryza sativa L.)–wheat (Triticum aestivum L.) cropping was studied in a long-term field experiment conducted in a wet temperate region of north India. Fresh lantana biomass was incorporated into the plough layer at 10, 20 and 30 Mg ha⁻¹ annually, 7–10 days before puddling. Plant-available water capacity (PAWC), non-limiting water range (NLWR) and NLWR:PAWC ratio were determined to characterize soil physical environment during wheat crop in the tenth cropping cycle.

Ten annual applications of lantana at 10, 20 and 30 Mg ha⁻¹, increased organic carbon (OC) content over control by 12.6, 17.6 and 27.9% in 0–15 cm soil layer, and 17.1, 26.3 and 39.5% in 15–30 cm soil layer, respectively. The OC content in 0–15 and 15–30 cm soil layer of control plots was 11.1 and 7.6 g kg⁻¹ soil. Bulk density decreased by 3–14% in 7.5–10.5 cm layer and 1–6% in 15–18 cm layer. Volumetric moisture contents at 10% air-filled porosity were 38.4, 40.0, 54.5 and 55.7% at 7.5–10.5 cm depth, and 31.4, 32.2, 33.9 and 34.6% at 15–18 cm depth corresponding to 0, 10, 20 and 30 Mg ha⁻¹ lantana treatment, respectively. At 15–18 cm soil depth, volumetric moisture contents at 2 MPa soil penetration resistance were 26.9, 24.8, 23.0 and 19.6% in zero, 10, 20 and 30 Mg ha⁻¹ lantana-treated plots, respectively. Lower soil water contents associated with 10% air-filled porosity and greater soil water contents associated with a limiting penetration resistance of 2 MPa resulted in a lower NLWR (4.3%) for control as compared to lantana-treated soil (7.4–15.1%). The PAWC showed slight increase from 12.9 to 13.4–14.9% due to lantana additions. The NLWR:PAWC ratio was also lower in control (0.33) as compared to lantana-treated soil (0.55–1.01). The NLWR was significantly and positively correlated with wheat grain yield (r=0.858^**).     

Tillage, crop residue and N fertilizer effects on crop yield, nutrient uptake, soil quality and nitrous oxide gas emissions in a second 4-yr rotation cycle

An 8-yr (1998–2005) field experiment was conducted on a Gray Luvisol (Boralf) soil near Star City, Saskatchewan, Canada, to determine the effects of tillage (no-tillage – NT and conventional tillage – CT), straw management (straw retained – R and straw not retained – NR) and N fertilizer (0, 40, 80 and 120 kg N ha⁻¹, except no N to pea (Pisum sativum L.) phase of the rotation) on seed and straw yield, mass of N and C in crop, organic C and N, inorganic N and aggregation in soil, and nitrous oxide (N₂O) emissions for a second 4-yr rotation cycle (2002–2005). The plots were seeded to barley (Hordeum vulgare L.) in 2002, pea in 2003, wheat (Triticum aestivum L.) in 2004 and canola (Brassica napus L.) in 2005. Seed, straw and chaff yield, root mass, and mass of N and C in crop increased with increasing N rate for barley in 2002, wheat in 2004 and canola in 2005. No-till produced greater seed (by 51%), straw (23%) and chaff (13%) yield of barley than CT in 2002, but seed yield for wheat in 2004, and seed and straw yield for canola in 2005 were greater under CT than NT. Straw retention increased seed (by 62%), straw (by 43%) and chaff (by 12%) yield, and root mass (by 11%) compared to straw removal for barley in 2002, wheat in 2004, and seed and straw yield for pea in 2003. No-till resulted in greater mass of N in seed, and mass of C in seed, straw, chaff and root than CT for barley in 2002, but mass of N and C were greater under CT than NT for wheat in 2004 and for canola in 2005 in many cases. Straw retention had greater mass of N and C in seed, straw, chaff and root in most cases compared to straw removal for barley in 2002, pea in 2003 and wheat in 2004. Soil moisture content in spring was higher under NT than CT and with R than NR in the 0–15 cm depth, with the highest moisture content in the NT + R treatment in many cases. After eight crop seasons, tillage and straw management had no effect on total organic C (TOC) and N (TON) in the 0–15 cm soil, but light fraction organic C (LFOC) and N (LFON), respectively, were greater by 1.275 Mg C ha⁻¹ and 0.031 Mg N ha⁻¹ with R than NR, and also greater by 0.563 Mg C ha⁻¹ and 0.044 Mg N ha⁻¹ under NT than CT. There was no effect of tillage, straw and N fertilization on the NH₄-N in soil in most cases, but R treatment had higher NO₃-N concentration in the 0–15 cm soil than NR. The NO₃-N concentration in the 0–15, 15–30 and 30–60 cm soil layers increased (though small) with increasing N rate. The R treatment had 6.7% lower proportion of fine (<0.83 mm diameter) and 8.6% greater proportion of large (>38.0 mm) dry aggregates, and 4.5 mm larger mean weight diameter (MWD) compared to NR treatment. This suggests a lower potential for soil erosion when crop residues are retained. There was no beneficial effect of elimination of tillage on soil aggregation. The amount of N lost as N₂O was higher from N-fertilized (580 g N ha⁻¹) than from zero-N (155 g N ha⁻¹) plots, and also higher in CT (398 g N ha⁻¹) than NT (340 g N ha⁻¹) in some cases. In conclusion, retaining crop residues along with no-tillage improved some soil properties and may also be better for the environment and the sustainability of high crop production. Nitrogen fertilization improved crop production and some soil quality attributes, but also increased the potential for NO₃-N leaching and N₂O-N emissions, especially when applied in excess of crop requirements.     

Diesel and glyphosate price changes benefit the economics of conservation tillage versus traditional tillage

Recent increases in diesel price and decreases in glyphosate [N-(phosphonomethyl) glycine] price should favor the profitability and farmer acceptance of herbicide-intensive conservation tillage systems versus fuel-intensive traditional tillage (TT) systems. Profitability results from a long-term field experiment that compared TT, minimum tillage (MT), and delayed minimum tillage (DMT) systems for winter wheat–(Triticum aestivum L.)summer fallow in eastern Washington, USA were calculated using both 1998 and 2005 input prices. Net returns for the MT and DMT systems increased by US$ 6.37 and 6.30 (rotational ha)⁻¹, respectively, and net returns to the TT system decreased by US$ 2.36 (rotational ha)⁻¹ when 2005 versus 1998 prices were used. Here, rotational ha equals 0.5 ha fallow and 0.5 ha wheat. Focusing on the dominant crop of soft white winter wheat (SWWW), the 2005 price hikes pushed diesel costs up for all systems, from US$ 6.81 (rotational ha)⁻¹ for DMT to US$ 9.00 (rotational ha)⁻¹ for TT. The cost of diesel for the conservation tillage systems, relative to the cost for TT, decreased by US$ 1.50–2.20 (rotational ha)⁻¹. The conservation tillage systems accrue greater savings from the price reduction in glyphosate because they consume more of this herbicide. An unanticipated result was that relative cost savings from price changes in N fertilizer rivaled those from diesel and glyphosate because anhydrous NH₃–N was exclusively used in the experiment for TT and aqueous NH₃–N for MT and DMT. The price of anhydrous NH₃–N increased from US$ 0.55 kg⁻¹ in 1998 to 0.85 kg⁻¹ in 2005, a 56% increase. Aqueous NH₃–N only increased from $0.75 kg⁻¹ in 1998 to 0.85 kg⁻¹ in 2005, a 15% increase. The greater price increase for anhydrous NH₃–N penalized the TT system because of its use of this fertilizer. If the same source of N fertilizer were used on all three tillage systems, this fertilizer cost effect would disappear. Nonetheless, the conservation tillage systems still retained a statistically significant profitability advantage over TT even if the same fertilizer was used throughout. The sharp price increase for diesel and the concurrent price decrease for glyphosate herbicide favored the conservation tillage systems over TT in this study. Results provide strong evidence for the superior profitability of conservation tillage winter wheat–summer fallow under current economic conditions.     

The impact of tillage and residual nitrogen on wheat

Wheat (Triticum aestivum L.) yield and quality is influenced by management of the previous crop but is highly dependent on current year management. The objective of this study was to evaluate the response of winter wheat seeded in two tillage systems [conventional tillage (CT) and no-till (NT)] to four N rates applied to a previous cotton (Gossypium hirsutum L.) crop (0, 67, 134, and 202 kg ha⁻¹). The experiment with wheat was conducted on a Dothan sandy loam (fine, loamy siliceous, thermic Plinthic Kandiudults) at the University of Florida North Florida Research and Education Center near Quincy, FL from 1995 to 1997. For most plant characteristics, there was a tillage x N x year interaction. Greater plant emergence (79.4 vs. 65.3%) and grain N (23.5 vs. 21.5 g kg⁻¹), and lower grain moisture (139 vs. 142 g kg⁻¹) were obtained under NT than CT, respectively, in one out of two years. Nitrogen applied to a previous cotton crop increased wheat grain yields, plant height and seed number under NT in 1995–1996 and CT in 1996–1997, head density under NT in both years, harvest index under CT in 1996–1997, and grain N concentration in 1995–1996 and 1996–1997 due to residual plant and soil N. With every 1 kg N applied to a previous cotton crop, wheat grain yields increased by 5.38 kg ha⁻¹ under NT, whereas grain yield under CT was not influenced by N application to cotton in 1995–1996. In 1996–1997, grain yields increased by 4.96 and 4.23 kg ha⁻¹ for wheat grown in NT and CT, respectively. Generally, wheat seeded in NT following cotton did not decrease stand or yields compared to CT and wheat grain yields and grain N content increased with N fertilization of the previous crop. However, we would have to apply about 134 kg N ha⁻¹ to a previous cotton crop to maximize wheat production under NT and CT.