共查询到20条相似文献,搜索用时 312 毫秒
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高通量测序技术以及全基因组深度测序技术的快速发展,加速了检测技术的更新步伐。文章对高通量测序技术的发展及在微生物检测领域应用进行综述,并对高通量测序技术检测猪病的实践应用进行了分析。 相似文献
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DNA测序技术从以Sanger法为代表的第一代测序技术到如今的高通量测序(HTS)技术,经历了漫长而曲折的发展过程,如今该技术在动植物基因组的研究中获得了很大的成功,它的问世可以使人们以更低廉的价格,更全面、更深入地对全基因组进行分析.作者阐述了几种关于HTS的测序技术平台:Roche/454测序技术、ABI/SOLID测序技术、Illumina/Solexa测序技术、单分子测序技术及Ion Torrent测序技术,并且还归纳了HTS在基因组学、转录组学及表观基因组学等方面的应用. 相似文献
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下一代测序(NGS),也被称为深度、高通量或大规模并行测序,可一次同时测定几十万到几百万条核酸分子序列。在畜禽疫病中应用于复杂诊断和密集监测、病因学、基因组学、进化和流行病学,以及宿主-病原体相互作用和感染生物学等方面。本综述首先简要介绍了深度测序技术,并通过实例对深度测序在畜禽疫病诊断中的应用进展进行阐述,为今后的相关研究提供些许参考。 相似文献
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F Stevens 《Veterinary Clinics of North America: Small Animal Practice》1986,16(4):685-702
The use of small computers for assistance in medical diagnosis is examined in light of their limitations, particularly in a veterinary clinical setting. Various approaches to these problems are discussed with an eye towards "appropriate technology" for the veterinary practice. The author describes two "expert systems" he devised that enable the small computer to aid in the analysis of laboratory data and in the clinical diagnosis of veterinary patients. 相似文献
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McMichael MA Smith SA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(2):140-153
Use of viscoelastic point-of-care (POC) coagulation instrumentation is relatively new to veterinary medicine. In human medicine, this technology has recently undergone resurgence owing to its capacity to detect hypercoagulability. The lack of sensitive tests for detecting hypercoagulable states, along with our current understanding of in vivo coagulation, highlights the deficiencies of standard coagulation tests, such as prothrombin and partial thromboplastin times, which are performed on platelet-poor plasma. Viscoelastic coagulation analyzers can provide an assessment of global coagulation, from the beginning of clot formation to fibrinolysis, utilizing whole blood. In people, use of this technology has been reported to improve management of hemostasis during surgery and decrease usage of blood products and is being used as a rapid screen for hypercoagulability. In veterinary medicine, clinical use of viscoelastic technology has been reported in dogs, cats, foals, and adult horses. This article will provide an overview of the technology, reagents and assays, applications in human and veterinary medicine, and limitations of the 3 viscoelastic POC analyzers in clinical use. 相似文献
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Dascanio JJ 《Journal of veterinary medical education》2003,30(4):326-330
To facilitate large animal veterinary education, information technology has to connect the classroom to the farm. Technology needs to be portable, durable, and cost-effective. Dairy production medicine was one of the first areas in veterinary medicine to use computers to manage large sets of milk production-related data. Now equine practice has embraced technology in almost every diagnostic imaging methodology. The personal desktop assistant (PDA) has allowed students to take large volumes of information with them during clinical rotations. The technology is advancing so quickly that it is difficult to keep up with new software and hardware developments, let alone purchase the most current versions. This article reviews information technology as it is used in large animal practice and, consequently, in large animal veterinary education. 相似文献
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《兽医内科学》是动物医学专业本科教育的专业方向课,具有较强的理论性、实践性和系统性,是动物医学专业的临床主干课程之一,也是动物临床医学的核心课。目前,该课程主要以线下双语教学方式为主,但随着信息化教育技术不断发展及国际交流合作越来越普及化,全线下教学模式局限性日渐凸显,尤其面对2020年初新冠疫情的暴发,线上居家教学成为教师和学生教与学的唯一方式。线下线上融合的双语教学能更充分的利用在线优质教育资源,对于实现资源共享、推动兽医教育与先进国家接轨、培养综合型高级兽医专业人才、增强国际竞争力具有重大意义。本文拟借助超星学习通平台探索一种教学效果相对理想的《兽医内科学》线上线下融合教学模式,并在实践教学中应用推广,主要从教学内容、教学模式、网络平台、师资队伍、教材开发等诸多方面对教学资源进行补充与创新、继续发展和完善,目标在于将《兽医内科学》双语教学发展到高水平阶段,以满足学生的学习和发展需求。 相似文献
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Artificial intelligence and machine learning have promising applications in several medical fields of diagnosis, imaging, and laboratory testing procedures. However, the use of this technology in the veterinary medicine field is lagging behind, and there are many areas where it could be used with potentially successful outcomes and results. In this study, two critical predictions were explored in horses presented with acute abdomen (colic) using this technology. Those were the need for surgical intervention and survivability likelihood of affected horses based on clinical data (history, clinical examination findings, and diagnostic procedures). The two prediction parameters were explored using the application of Decision Trees, Multilayer Perceptron, Bayes Network, and Naïve Bayes. The machine learning algorithms were able to predict the need for surgery and survivability likelihood of horses presented with acute abdomen (colic) with 76% and 85% accuracy, respectively. The application of this technology in the different clinical fields of veterinary medicine appears to be of a value and warrants further investigation and testing. 相似文献
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Simulation technology will feature prominently in this exciting, yet challenging, time for veterinary medicine. The profession is evolving to keep pace with rapid changes in clinical practice, scientific discovery, and educational strategy, while ensuring that it follows the public mandate to produce competent veterinarians. Among the challenges to meeting this educational goal are limitations-due to important issues such as animal welfare-on the availability of real patients for training. Drawing chiefly on the experience in human medicine, this article explores the use of simulations in veterinary medical education to provide safe and ethical alternative opportunities for learners to practice essential clinical and professional skills. 相似文献
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Reasons for performing study: Assessing patients' quality of life (QOL) is a core part of clinical decision making. Various methodologies for assessing patients' QOL have been developed in human medicine and small animal veterinary disciplines. In contrast, the lack of aids for QOL assessment in equine veterinary practice leaves practitioners reliant on subjective assessments of QOL, which may be prone to avoidable errors. Objectives: This paper suggests pragmatic ways in which QOL may be enhanced, while remaining appropriate for the time, financial and owner‐based constraints within equine practice. Methods: Through interdisciplinary research, this paper identifies, adapts and applies insights from several areas of research and practical experience in order to develop an overarching approach to making QOL‐based decisions in clinical cases. Results: The paper identifies 6 steps involved in QOL‐based decision making and provides examples of how these steps may be practically applied. These include deciding what each clinician feels is important; deciding how to evaluate it, including taking owners' views into consideration; making decisions about each case and achieving the desired clinical outcomes. Conclusions: Practitioners can draw their own conclusions on how they may improve QOL assessment in practice and may usefully share these with colleagues. Reporting cases and sharing practical examples of QOL tools used on the ground are vital to the development of this field and appropriate methodologies. Potential relevance: Improvements in QOL assessment are relevant to all areas of equine veterinary practice, and several areas of research. Further research may develop QOL assessment in practice, but more important are the personal improvements that each practitioner may achieve. See also correspondence by Grove 相似文献