水稻僵苗的原因及防治对策

水稻秧苗僵苗的症状表现是植株矮小,叶片细瘦,直立不披散,稻丛呈簇立状,根细而稀,新根少。根据秧苗初期因僵苗程度不同,长相长势有所区别,大致可分3个阶段：发僵,为黄化阶段,叶色较淡,矮小萎黄,锈根多,白根少,呈现出营养不良的症状;以后随着生理缺磷程度的加重,进入蓝化阶段，叶色暗绿发蓝，有的黄中带红，叶片沿纵脉呈环状卷曲，老根直伸向下，根毛稀少，这是由于生理缺磷影响能量代谢，使光合作用产物的运输受到严重阻碍导致的；僵苗严重时为红化阶段，老叶变红色，其余叶片除最新抽出叶呈全绿色外，均有不同程度的叶尖枯焦，远看苗色发红。     

Long-term variations in runoff of the Syr Darya River Basin under climate change and human activities

In this study,we analyzed the hydrological and meteorological data from the Syr Darya River Basin during the period of 1930–2015 to investigate variations in river runoff and the impacts of climate change and human activities on river runoff.The Syr Darya River,which is supplied by snow and glacier meltwater upstream,is an important freshwater source for Central Asia,as nearly half of the population is concentrated in this area.River runoff in this arid region is sensitive to climate change and human activities.Therefore,estimation of the climatic and hydrological changes and the quantification of the impacts of climate change and human activities on river runoff are of great concern and important for regional water resources management.The long-term trends of hydrological time series from the selected 11 hydrological stations in the Syr Darya River Basin were examined by non-parametric methods,including the Pettitt change point test and Mann-Kendall trend tests.It was found that 8 out of 11 hydrological stations showed significant downward trends in river runof f.Change of river runoff variations occurred in the year around 1960.Moreover,during the study period(1930–2015),annual mean temperature,annual precipitation,and annual potential evapotranspiration in the river basin increased substantially.We employed hydrological sensitivity method to evaluate the impacts of climate change and human activities on river runoff based on precipitation and potential evapotranspiration.It was estimated that human activities accounted for over 82.6%–98.7%of the reduction in river runoff,mainly owing to water withdrawal for irrigation purpose.The observed variations in river runoff can subsequently lead to adverse ecological consequences from an ecological and regional water resources management perspective.     

实施植物检疫行政许可中的问题及改进建议

国内农业植物检疫（以下简称植物检疫）是以法律法规为依据、检疫技术为后盾、植物检疫行政许可和执法监督为保障来控制农业有害生物传播、蔓延、危害,达到促进农产品流通,保护农业生态,确保农业生产高产、优质、高效的目的。植物检疫行政许可在植物检疫工作中起着关键的作用。现把江山市2005年以来贯彻实施《行政许可法》和农业部有关农业行政许可规定过程中的有关情况概述如下。     

加拿大一枝黄花不同成熟度种子的发芽率

在加拿大一枝黄花盛花期,采用剪除花穗、剪除花穗并短截、砍伐植株,以后再采收加拿大一枝黄花种子,于次年进行种子发芽试验.结果表明,3种处理的加拿大一枝黄花种子发芽率均在0.2%～0.4%之间,与对照有极显著差异.     

Coupling analysis of social-economic water consumption and its effects on the arid environments in Xinjiang of China based on the water and ecological footprints

In arid areas,ecological degradation aroused by over-exploitation of fresh water,expansion of artificial oasis and shrinkage of natural oasis,has drawn attention of many scholars and officials.The water and ecological footprints can be used to quantitatively evaluate the water consumption of social-economic activities and their influence on the eco-environments.In addition,increase of the water footprint indicates the expansion of artificial oasis,and the influence on the natural oasis could be reflected by the variation of the ecological footprint.This study was conducted to answer a scientific question that what is the quantitative relationship between the expansion of the artificial oasis and the degradation of the natural oasis in the arid environments of Xinjiang,China.Thus,based on the social-economic data,water consumption data and meteorological data during 2001–2015,we calculated the water and ecological footprints to express the human-related pressure exerted on the water resources and arid environments in Xinjiang(including 14 prefectures and cities),and explore the relationship between the water and ecological footprints and its mechanism by using the coupling analysis and Granger causality test.The results show that both the water and ecological footprints of Xinjiang increased significantly during 2001–2015,and the increasing rate of the ecological footprint was much faster than that of the water footprint.The coupling degree between the water and ecological footprints was relatively high at the temporal scale and varied at the spatial scale.Among the 14 prefectures and cities examined in Xinjiang,the greater social-economic development(such as in Karamay and Urumqi)was associated with the lower coupling degree between the two footprints.Increases in the water footprint will cause the ecological footprint to increase,such that a 1-unit increase in the consumption of water resources would lead to 2–3 units of ecological degradation.The quantitative relationship between the increases of the water and ecological footprints,together with the intensities of water consumption both in the natural and artificial oases of Tarim River Basin,have approved the fact that the formation and expansion of 1 unit of the artificial oasis would bring about the degradation of 2 units of the natural oasis.These conclusions not only provide a technical basis for sustainable development in Xinjiang,but also offer a theoretical guide and scientific information that could be used in similar arid areas around the world.     

Modeling and analyzing supply-demand relationships of water resources in Xinjiang from a perspective of ecosystem services

Water shortage is one bottleneck that limits economic and social developments in arid and semi-arid areas.As the impacts of climate change and human disturbance intensify across time,uncertainties in both water resource supplies and demands increase in arid and semi-arid areas.Taking a typical arid region in China,Xinjiang Uygur Autonomous Region,as an example,water yield depth(WYD)and water utilization depth(WUD)from 2002 to 2018 were simulated using the Integrated Valuation of Ecosystem Services and Tradeoffs(InVEST)model and socioeconomic data.The supply-demand relationships of water resources were analyzed using the ecosystem service indices including water supply-demand difference(WSDD)and water supply rate(WSR).The internal factors in changes of WYD and WUD were explored using the controlled variable method.The results show that the supplydemand relationships of water resources in Xinjiang were in a slight deficit,but the deficit was alleviated due to increased precipitation and decreased WUD of irrigation.WYD generally experienced an increasing trend,and significant increase mainly occurred in the oasis areas surrounding both the Junggar Basin and Tarim Basin.WUD had a downward trend with a decline of 20.70%,especially in oasis areas.Water resources in most areas of Xinjiang were fully utilized and the utilization efficiency of water resources increased.The water yield module in the InVEST model was calibrated and validated using gauging station data in Xinjiang,and the result shows that the use of satellite-based water storage data helped to decrease the bias error of the InVEST model by 0.69×10⁸m³.This study analyzed water resource supplies and demands from a perspective of ecosystem services,which expanded the scope of the application of ecosystem services and increased the research perspective of water resource evaluation.The results could provide guidance for water resource management such as spatial allocation and structural optimization of water resources in arid and semi-arid areas.     

How precipitation and grazing influence the ecological functions of drought-prone grasslands on the northern slopes of the Tianshan Mountains,China?

Drought-prone grasslands provide a critical resource for the millions of people who are dependent on livestock for food security.However,this ecosystem is potentially vulnerable to climate change(e.g.,precipitation)and human activity(e.g.,grazing).Despite this,the influences of precipitation and grazing on ecological functions of drought-prone grasslands in the Tianshan Mountains remain relatively unexplored.Therefore,we conducted a systematic field investigation and a clipping experiment(simulating different intensities of grazing)in a drought-prone grassland on the northern slopes of the Tianshan Mountains in China to examine the influences of precipitation and grazing on aboveground biomass(AGB),soil volumetric water content(SVWC),and precipitation use efficiency(PUE)during the period of 2014–2017.We obtained the meteorological and SVWC data using an HL20 Bowen ratio system and a PR2 soil profile hydrometer,respectively.We found that AGB was clearly affected by both the amount and seasonal pattern of precipitation,and that PUE may be relatively low in years with either low or excessive precipitation.The PUE values were generally higher in the rapid growing season(April–July)than in the entire growing season(April–October).Overall,moderate grazing can promote plant growth under water stress conditions.The SVWC value was higher in the clipped plots than in the unclipped plots in the rapid growing season(April–July),but it was lower in the clipped plots than in the unclipped plots in the slow growing season(August–October).Our findings can enhance the understanding of the ecological effects of precipitation and grazing in drought-prone grasslands and provide data that will support the effective local grassland management.     

Ecosystem service values of gardens in the Yellow River Basin,China

Studies on the ecosystem service value(ESV)of gardens are critical for informing evidence-based land management practices based on an understanding of the local ecosystem.By analyzing equivalent value factors(EVFs),this paper evaluated the values of 11 ecosystem services of gardens in the Yellow River Basin of China in 2019.High-precision land use survey data were used to improve the accuracy of the land use classification,garden areas,and spatial distribution of the ESVs of gardens.The results showed that garden ecosystem generally had high ESVs,especially in terms of the ESV of food production,which is worthy of further research and application to the practice of land use planning and management.Specifically,the value of one standard EVF of ecosystem services in 2019 was 3587.04 CNY/(hm²·a),and the ESV of food production of gardens was much higher than that of croplands.Garden ecosystem provided an ESV of 1348.66×10⁸CNY/a in the Yellow River Basin.The areas with the most concentrated ESVs of gardens were located in four regions:downstream in the Shandong-Henan zone along the Yellow River,mid-stream in the Shanxi-Shaanxi zone along the Yellow River,the Weihe River Basin,and upstream in the Qinghai-Gansu-Ningxia-Inner Mongolia zone along the Yellow River.The spatial correlation of the ESVs in the basin was significant(global spatial autocorrelation index Moran's I=0.464),which implied that the characteristics of high ESVs adjacent to high ESVs and low ESVs adjacent to low ESVs are prominent.In the Yellow River Basin,the contribution of the ESVs of gardens to the local environment and economy varied across regions.We also put forward some suggestions for promoting the construction of ecological civilization in the Yellow River Basin.The findings of this study provide important contributions to the research of ecosystem service evaluation in the Yellow River Basin.     

水葫芦的一种黑斑病鉴定记述

重庆地区水葫芦上发现一种叶部病害,通过对病原菌分离、纯化获得一种真菌,再根据科赫氏证病律步骤进行病害诊断,确定了病原菌.病害的典型症状是在被侵染的植株叶片上出现黄色小圆斑,以后病斑扩大为椭圆形,或多个病斑合并成不规则形.病斑中央黑褐色,周围有明显的黄色晕圈带.病原真菌为链格孢(Alternaria sp.),在马铃薯蔗糖琼脂(PSA)上菌落生长良好,表面气生菌丝绒毛状,后期菌落黑褐色.常规培养条件下约20d产生少量分生孢子.分生孢子深褐色,分1～3横格,短喙或无喙.接种初步观察,该病原真菌的致病性很强,在水葫芦的生物控制中可能具有重要的应用前景.     

Interactions between vegetation dynamic and edaphic factors in the Great Salt Desert of central Iran

Investigating the relationships between vegetation dynamic and edaphic factors provide management insights into factors affecting the growth and establishment of plant species and vegetation communities in saline areas.The aim of this study was to assess the spatial variability of various vegetation communities in relation to edaphic factors in the Great Salt Desert,central Iran.Fifteen vegetation communities were identified using the physiognomy-floristic method.Coverage and density of vegetation communities were determined using the transect plot method.Forty soil samples were collected from major horizons of fifteen profiles in vegetation communities,and analyzed in terms of following soil physical and chemical characteristics:soil texture,soluble Na+concentration,sodium adsorption ratio(SAR),electrical conductivity(EC),pH,organic matter content,soluble Mg2+and Ca2+concentrations,carbonate and gypsum contents,and spontaneously-and mechanically-dispersible clay contents.Redundancy analysis was used to investigate the relationships between vegetation dynamic and edaphic factors.The generalized linear method(GLM)was used to find the plant species response curves against edaphic factors.Results showed that plant species responded differently to edaphic factors,in which soluble sodium concentration,EC,SAR,gypsum content and soil texture were identified as the most discriminative edaphic factors.The studied plant species were also found to have different ecological requirements and tolerance to edaphic factors,in which Tamarix aphylla and Halocnemum strobilaceum were identified as the most salt-resistant species in the region.Furthermore,the presence of Artemisia sieberi was highly related to soil sand and gypsum contents.The results implied that exploring the plant species response curves against edaphic factors can assist managers to lay out more appropriate restoration plans in similar arid areas.     

Localization of metabolic sites of action of herbicides

Time- and concentration-course studies were conducted to determine the effect of thirteen herbicides on photosynthesis, respiration, RNA synthesis, protein synthesis, and lipid synthesis using isolated single leaf cells. Each herbicide was from a different chemical class. Appropriate ¹⁴C-substrates and product purification procedures were used for each process prior to liquid scintillation counting. The most sensitive metabolic site of inhibition was photosynthesis for atrazine, bromacil, dichlobenil, monuron, and paraquat; RNA synthesis for dalapon and dinoseb; protein synthesis for chlorpropham; and lipid synthesis for CDAA, chloramben, 2,4-D, EPTC, and trifluralin. However, with several herbicides, one or more process was almost as sensitive as the one mentioned above. All herbicides inhibited more than one process, and the most sensitive site of inhibition may not be the same process that was inhibited the greatest at the maximum concentration and maximum exposure time used. Therefore, a concept of metabolic sites of action, rather than a primary site of action, appears to be more meaningful for herbicides.     

Effects of timing and method of application of Penicillium oxalicum on efficacy and duration of control of Fusarium wilt of tomato

The effects of timing and method of application of Penicillium oxalicum on the control of fusarium wilt of tomato were investigated. Application of P. oxalicum to tomato seedlings in seedbeds reduced disease caused by Fusarium oxysporum f.sp. lycopersici in a growth chamber by 45–49% and in glasshouse experiments by 22–69%. Disease suppression was maintained for 60–100 days after inoculation with the pathogen in the glasshouse. No disease reduction was observed in tomato plants where P. oxalicum was applied to seeds. Treatment with P. oxalicum did not affect the population of F. oxysporum f.sp. lycopersici in the rhizosphere.     

福寿螺配偶个体大小选择性初步观察

通过野外观察与实验研究,掌握了福寿螺的婚配体制及其配偶选择性规律。福寿螺与多数低等动物一样,其婚配属于乱交制,无固定配偶;雌螺对与其交配的雄螺个体大小没有选择性,而雄螺对雌螺的个体大小有选择性,倾向于与较大个的雌螺交配。     

Systems of designation of pathotypes of plant pathogens12

A variety of systems of designation has evolved to name pathotypes of plant pathogens. The systems were evaluated to determine those best suited for particular purposes. Virulence and avirulence/virulence formulae of pathotypes have advantage over the use of consecutive numbers or letters given in chronological order of pathotype discovery. As soon as pathotype information exceeds a certain level of complexity, mathematical codes are most advantageous, in particular two codes, octal notation and coded triplets. A more universal adoption of the most appropriate codes is recommended to ease communication and comparisons of results.     

Investigation of the Mode of Action of Nematode Neuropeptides

The neuropeptide AF2 has a complex set of actions on the dorsal muscle strip of Ascaris suum, including a potentiation of the acetylcholine-stimulated muscle contraction. Caffeine at 100 μm and 5 mm inhibited this potentiation, as did 100 μm theophylline in two out of six studies. The cyclic-AMP-potentiating compounds IBMX, dibutyryl cAMP and forskolin had no effect on the AF2-induced potentiation of the acetylcholine-stimulated muscle contraction. These preliminary data suggest that the potentiating action of AF2 is not mediated by a cAMP pathway.     

百合地下害虫主要种类调查及生物（源）农药筛选

调查了甘肃省临洮县为害百合的地下害虫种类, 并以主要发生为害种类为供试对象, 采用拌土法测定了几种生物（源）农药对主要害虫的室内毒力和致死中时。调查发现小云斑鳃金龟Polyphylla gracilicornis、棕色鳃金龟Holotrichia titanis为主要发生及为害种类, 不同时间发生种类存在差异, 9月份以小云斑鳃金龟幼虫发生为害为主, 发生量占地下害虫总数的66.16%, 平均种群密度为10.48头/m2, 10月份, 则以棕色鳃金龟幼虫发生为主, 发生量占地下害虫总数的96.86%, 平均种群密度为7.79头/m2, 主要取食百合根系和鳞茎, 造成根系数量减少, 鳞茎出现不规则褐色缺口或凹陷斑。药剂筛选结果表明, 1.8%阿维菌素乳油、200亿孢子/g球孢白僵菌粉剂对两种蛴螬均具有较高的杀虫活性和速效性, 药后7 d时, 校正死亡率在82.22%以上, 对小云斑鳃金龟幼虫和棕色鳃金龟幼虫的LC50分别为0.161、0.060 mg/g和5.558×107、0.362×107孢子/g, LT50分别为2.237 d（2.2 mg/g）、1.393 d（2.2 mg/g）和6.645 d（40.0×107孢子/g）、4.940 d（2.0×107孢子/g）, 这两种生物（源）农药对两种蛴螬的LC50和LT50值均略大于两种化学农药25%二嗪磷乳油、40%辛硫磷乳油处理的, 且对小云斑鳃金龟幼虫的杀虫活性均低于对棕色鳃金龟幼虫的杀虫活性, 致死中时则长于对棕色鳃金龟幼虫的致死中时。     

厚朴病虫害种类的初步调查

采用标准地法和线路调查法,对湖北恩施市新塘乡双河厚朴基地的厚朴病虫害进行了系统调查,记录主要虫害13种,其中叶部害虫9种,枝干害虫2种,根部害虫2种。厚朴主要病害5种。藤壶蚧、厚朴枝角叶蜂和厚朴新丽斑蚜为湖北省首次报道,小绿叶蝉为厚朴新寄主记录种。同时记录了藤壶蚧的天敌6种,其中寄生小蜂2种,瓢虫4种;厚朴新丽斑蚜的天敌昆虫8种。对藤壶蚧、厚朴枝角叶蜂和厚朴苗木根腐病等重要病虫害的发生规律进行了初步调查,同时提出了防治建议。     

青稞种子带菌检测及杀菌剂消毒处理效果

为了研究青稞种子外部和内部携带真菌情况,比较不同杀菌剂对青稞种子的带菌消毒效果和对幼苗生长的影响,为青稞种子播前包衣处理和种传真菌病害防控提供依据,采用离体平皿法对云南迪庆‘云青1号’、‘云青2号’和‘短白青稞’3个主栽品种进行带菌检测,并对种子进行拌种或浸种处理测定6种杀菌剂对种子消毒效果,分析杀菌剂对种子发芽和幼苗生长的影响。结果表明:供试青稞种子表面携带的优势菌群为青霉(Penicilliumspp.)、镰刀菌(Fusariumspp.);种子内部寄藏的真菌主要为镰刀菌、核腔菌(Pyrenophoraspp.)、附球菌(Epicoccumspp.)、丝核菌(Rhizoctoniaspp.)、链格孢(Alternariaspp.)和木霉(Trichoderma spp.)。青稞不同品种的种子表面及内部携带的真菌种类差异较大。致病性测定表明,镰刀菌对种子萌发和幼苗生长影响最大,后期出现幼苗坏死现象。45%咪鲜胺EW、75%百菌清WP、50%福美双WP对青稞种子携带真菌均有显著抑制作用和消毒效果,50%福美双WP消毒效果最优,达100%;45%咪鲜胺EW、75%百菌清WP、50%福美双WP处理对青稞种子发芽和幼苗生长均无显著影响。     

棉花黄萎病菌致病型的AFLP分析

 选用41个棉花黄萎病菌(Verticillium dahliae)代表菌系,在温室条件下,对4个棉花品种鄂荆1号(感)、中棉所12(耐)、文-5(抗)和唐棉2号(抗)进行致病性测定,结果可将供试菌系分为落叶型与非落叶型2类。选取8对AFLP引物PCR扩增的结果中,统计带型稳定、清晰且有多态性的条带,共169条作系统聚类分析,将上述菌系分为2大类,第一类为非落叶型菌系,包括10个非落叶型菌系和1个过渡菌系;第二类为30个落叶型菌系。根据聚类分析建立树状图,发现菌系与地理来源存在一定的相关性,而依据菌系致病力强弱分类则相关关系不大。选用25对EcoRⅠ和MseⅠ引物组合,对供试的41个V.dahliae进行AFLP扩增,筛选到2对引物E64(GACTGCGTACCAATTCGAC)、M53(GATGAGTCCTGAGTAACCG)和E49(GACTGCGTACCAATTCCAG)、M65(GAT-GAGTCCTGAGTAAGAG),能分别扩增出433bp和110bp2条仅为V.dahliae非落叶型菌系独有的特异片段,可将落叶型与非落叶型菌系分开,这2条特异片段被命名为EM₄₃₃和EM₁₁₀。