大花葵花色苷抑菌功能研究

本项研究通过采用固体培养和液体培养的试验方法,进行大花葵花(Malvasylvestris)色苷对金黄色葡萄球菌、大肠杆菌和黑曲霉的抑菌试验。结果表明:大花葵花色苷对金黄色葡萄球菌有很强的抑制作用,而对大肠杆菌和黑曲霉没有抑制现象发生。在固体培养试验中大花葵花色苷随着浓度的增加,对金黄色葡萄球菌的抑制程度越大,当大花葵花色苷浓度为10g/L时抑菌圈平均直径为6mm,当浓度增加至30g/L时,抑菌圈平均直径为16mm。而且,高浓度的大花葵花色苷溶液对金黄色葡萄球菌有长时间的抑制作用。     

Functional evaluation for effective compositions in seed oil of Korean pine

The effect of seed oil of Korean pine (Pinus koraiensis) on the rats’ blood-fat and its anti-ageing function was studied for appraising the efficacy of the seed oil of Korean pine. Sixty experimental rats were randomly divided into 5 groups (half males and half females in each group) as normal control group, high fat diet control group, and three groups (Group 1 Group 2, Group 3) that were fed with feedstuff with the contents of the seed oil of 2.0g/(kg·d⁻¹), 4.0g/(kg·d⁻¹) and 8.0g/(kg·d⁻¹), respectively. The indexes such as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and anti-oxidation capacity (AOC) were measured by Reagent Kit method. It was found that the seed oil of the Korean pine could reduce the content of triglyceride and improve SOD as well as GSH-PX activity in serum. These indexes of the rats in Group 2 fed with Korean pine seed oil of 4.0 g/(kg·d⁻¹) reached the significant level and those of rats in Group 3 fed with the seed oil of 8.0 g/(kg·d⁻¹) reached the extremely significant level. The results indicated the seed oil of Korean pine had function of regulating the level of blood-fat and anti-ageing. Biography: WANG Zhen-yu (1957-), male, Prof. in Forestry College, Northeast Forestry University, Harbin 150040, P. R. China. Responsible editor: Zhu Hong     

Embryo culture and rapid propagation of Syringa

Embryo of lilacs (Syringa L) culture in vitro and the rapid propagation were studied. The orthogonal experiments, including the selection of basal medium, embryo age and other factors such as sugar, benzyladenine (BA), naphthalene acetic acid (NAA) and glutamine (Gln), were carried out. The results indicated that the optimal medium for embryo culture was Monnier medium supplemented with NAA (0.001 mg.L^-1), BA (0.1 mg.L^-1), sugar (50 g.L^-1), and Gin (400 mg.L^-1), with a germination rate of 91.7% at least; the optimal embryo age was 50 d; and Gln had significant effects on the germination rate of embryo.Moreover, the optimal medium for subculture was MS BA (2 mg.L^-1) NAA (0.001 mg.L^-1) Gln (0.5 mg.L^-1), with the propagation coefficient of 3.6 at least.     

落叶松水浸液对胡桃楸幼苗生长的影响

A study was conducted to detect the effect of water extracts from different parts such as root, bark, branch and leaf, of adult larch, Larix gmelini, trees on growth of Manchurian walnut, Juglans mandshudca, seedlings and the allelopathy between the two tree species. Four concentrations （100 g. kg i, 50 g. kg^-1, 25 g. kg^-1 and 12.5 g. kg^-1） were prepared for each kind of extracts. Result showed that the water extracts with low and moderate concentrations accelerated the growth of collar diameter and increased biomass and root/shoot ratio of walnut seedlings. The water extracts from branches and barks with low and moderate concentrations accelerated the height growth of the seedlings, while those from leaves and roots slightly decreased the height growth of seedlings. The fact that application of water extracts of larch improved the growth of Manchurian walnut attributes possibly to the allelopathy between the two tree species.     

A study on the shade tolerance ofMuehlewbeckia complera

Muehlewbeckia complera was introduced to China in 2002 as indoor-hanging ornamental foliage plant. The experiment of the shade tolerance for this species was carried out in different light intensities (0.14–946.00 μmol·m⁻²·s⁻¹). After 40 days in experimental areas, leaf photosynthentic characteristics indexes ofM. complera in different photosynthesis active radiation (PAR) were measured with LI-COR6400 apparatus, such as the light compensation point, light saturation point, and maximum net photosynthesis rate, at the same time, the increments of total leaf area and leaf amount were measured. The results showed that the optimum light intensity range forM. complera was from 9.26 μmol·m⁻²·s⁻¹ to 569.00 μmol·m⁻²·s⁻¹ (463–28150 lx, relative humidity (RH) for 46–60%, temperature at 16–22°C). Under this condition, leaf photosynthetic efficiency was tiptop. AlthoughM. complera belonged to the moderate sun-adaptation plant species, the plant growth was inhibited when PAR increased to the level of 569.000 μmol·m⁻²·s⁻¹ or above.M. complera could sprout new leaves in photosynthesis active radiation of 0.16–19.22 μmol·m⁻²·s⁻¹ (8–961 lx), or 10 μmol·m⁻²·s⁻¹ for above 6 h. Foundation item: This study was supported by the Research Foundation of Northeast Forestry University. Biography: YUE Hua (1962-), female, Associate professor in Northeast Forestry University, Harbin 150040, P. R. China. Responsible editor: Zhu Hong     

孟加拉哈提亚地区海岸造林对土壤性质的影响

在孟加拉诺阿卡利地区及相临裸地,对海岸植被(12年和17年生无瓣海桑Sonneratia apetala)进行探索性研究,以便了解海岸造林对土壤特性的影响.在三种不同地带(内陆、中部、海边),在12年生和17年生无瓣海桑林下,土壤深度分别为0-10,10-30和30-40cm,土壤湿度、土壤粒度、有机质、C含量、总N、pH、有效P、K、Na、Ca和Mg含量明显高(p≤0.05,p≤0.01,p≤0.001)于其相临裸地的数据,土壤含盐量明显(p≤0.01)低于其相临裸地的数据.在内陆CharAlim植被,土壤表面的土壤湿度,土壤粒度,有机质,C含量、总N、pH、土壤含盐量、有效P、K、Na、Ca和Mg含量分别为:31.09%、2.24 g·cm-3、2.41%、4.14%、0.58%、7.07、O.09 dS·cm-1、28.06 mg·L-1、O.50 mg·L-1、11.5 mg·L-1、3.30 mg·L-1和2.7 mmol·kg-1;而在相邻的Char Rehania贫瘠地区的相同土壤深度,其相关值分别为:16.69%、1.25g·cm-3、O.43%、0.74%、O.25%、6.57、0.13 dS·cm-1、13.07mg·L-1、O.30mg·L-1、1.4 mg·L-1、O.30 mmol·kG-1和0.50 nag·L-1.然而,在小内陆到海边的植被中,土壤湿度、土壤密度、有机质、C含量、总N、pH、有效P、K、Na和Ca含量逐渐降低,而土壤含盐量、Na和Mg含量却逐渐增加.虽然,在植被与相临裸地的不同土壤深度中土壤质地不同,植被地中砂土份额明显(p≤0.01)低于相临裸地,而粉砂土份额则明显(p≤0.001)高于裸地.在本研究中,所有参数的评价也在为其他地区相关研究得到应用.     

Effects of Ca^2＋concentrations on accumulations of mineral elements in the components of Pteroceltis tatarinowii

The bark ofPteroceltis tatarinowii is a raw material for manufacturing Xuan Paper. The effects of Ca²⁺ concentrations on the accumulation of mineral elements in the bark, leaf and root ofPteroceltis tatarinowii were studied under controlled conditions. The types of Hoagland nutrient solution with three Ca²⁺ concentrations levels (200, 400 and 600 μg·g⁻¹) and a control (without Ca²⁺ were designed to culturePteroceltis tatarinowii. After 6 months, contents of seven mineral elements including Ca, K, Mg, Mn, Zn, Cu and Na in the root, leaf and bark were analyzed. The results indicated that Ca accumulations content in the root, leaf and bark had positively relation with Ca²⁺ concentrations (200, 400, 600 μg·g⁻¹), and the order of the Ca content in the three components was root>leaf>bark. Ca content in the root treated with 600 μg·g⁻¹ Ca²⁺ concentrations was 5.5 times as high as that of the control, and about 1.4 times as high as that of the root treated in 200 and 400 μg/g Ca²⁺ concentrations respectively. On the contrary, K and Mg contents in the root, leaf and bark were negatively related to Ca²⁺ concentrations, especially in the bark, and their accumulation trend followed the order of leaf>root>bark. K content in the bark treated with 600 μg·g⁻¹ Ca²⁺ concentrations was 39.3% of that of the control, and was 79.0% and 91.8% of that of the bark treated with 200μg·g⁻¹ and 400μg·g⁻¹ Ca²⁺ concentrations respectively; Mg content in the bark treated with 600μg·g⁻¹ Ca²⁺ concentrations was 23.4% of that of the control, and was 27.1% and 35.4% of that of the bark treated with 200 and 400 μg·g⁻¹ Ca²⁺ concentrations respectively. Compared with the control, the general tendency of Mn, Zn and Cu content decreased with increasing of Ca²⁺ concentrations and their contents were in the order: root>leaf>bark. Based on the results of this study, the experiment has been useful for providing academic bases in improving the bark quality ofPteroceltis tatarinowii on non-limestone soil. Foundation item: This paper is supported by National Natural Science Foundation of China (No. 39970608). Biography: Fang Shengzun (1963), male, Professor in Stiiviculture, Nanjing Forestry University, Nanjing 210027, P.R. China. Responsible editor: Zhu Hong     

Agroforestry in the Bolivian Altiplano: evaluation of tree species and greenhouse growth of wheat on soils treated with tree leaves

Severe environmental problems encountered in the highlands of Bolivia may be remedied through the adoption of agroforestry systems, never before studied adequately in this region. As a first step, seven tree species were tested for growth, survival and health at two elevations in the Bolivian altiplano. Species responded variably with Buddleja coriacea Remy., Pinus radiata D. Don. and Eucalyptus globulus Labill. (at the higher elevation) and E. globulus, Baccharis spp., Robinia pseudoacacia L. and B. coriacea (at the lower elevation), displaying high survival, growth and health. In a related greenhouse study, grain yields of wheat planted in soils amended with incorporated foliage of B. coriacea, P. radiata and E. globulus increased three-fold (0.3 g·plant⁻¹ to >1.0 g·plant⁻¹) over grain yields in unamended soils (B. coriacea > P. radiata = E. globulus). Grain nitrogen (mg·plant⁻¹) increased equally in soils amended with P. radiata and B. coriacea foliage (18 mg N⋅plant⁻¹ to 20 mg·plant⁻¹) but decreased in soils amended with foliage of E. globulus (18 g·plant⁻¹ to 9 g·plant⁻¹). This revised version was published online in June 2006 with corrections to the Cover Date.     

Control of Lymantria dispar L. by biological agents

The experiment on control ofLymantria dispar L by using different kinds of biological measures, including nuclear polyhedrosis virus (NPV) ofLymantria dispar L., BtMP-342, sex-attractant as well as botanical insecticide, was carried out in the forest regions of Inner Mongolia in 2003. Two concentrations (2.632×10⁶ PIB·ml⁻¹ and 2.632×10⁷ PIB·ml⁻¹) ofLymantria dispar L. NPV were sprayed on the 2rd-instar-larvae ofL. dispar and 70% and 77.8% control effect were obtained respectively. BtMP0-342 was applied to the 3rd- and 4th-instar larvae and the control effect was around 80%. The sex-attractant provided by Canada Pacific Forestry Research Center also showed a good result in trappingL. dispar adults. The self-produced botanical insecticide, which was extracted from a kind of poisonous plant distributed in Daxing'an Mountains, China, exhibited a good control result in controlling the larvae ofL. dispar, and 82% mortality was observed when spraying primary liquid of the botanical insecticide on the 3rd–5th-instar-larvae in lab. Foundation item: This paper was supported by Natural Science Foundation of Heilongjiang Province (C0235) and Youth Foundation of Harbin City (0061218024). Biography: ZHANG Guo-cai (1964), male, Ph. Doctor Candidate, associate professor in School of Forestry, Northeast Forestry University, Harbin 150040, P. R. China. Responsible editor: Song Funan     

分月扇舟蛾颗粒体病毒生物测定

从黑龙江省双城林场采集健康的分月扇舟蛾2龄幼虫,将室内保存一年的分月扇舟蛾虫尸研碎,分离提纯得到纯净的颗粒体病毒。将病毒悬液稀释为1..58×103PIB·mL-1,1.58×104PIB·mL-1,1.58×105PIB·mL-1,1.58×106PIB·mL-1,1.58×107PIB·mL-1,1.58×108PIB·mL-1,1.58×109PIB·mL-17种浓度,采集新鲜的杨树叶分别浸入上述不同浓度的悬液,对采集的健康幼虫进行接毒。9天实验结果表明,浓度为1..58×103PIB·mL-1时校正死亡率最小(7.32%),浓度是1.58×109PIB·mL-1时校正死亡率最大(97.36%),浓度对数和死亡几率值的回归直线方程为y=1.946 0.558x,LC50为2.97×105PIB·mL-1。1.58×105PIB·mL-1,1.58×106PIB·mL-1,1.58×107PIB·mL-1,1.58×108PIB·mL-1,1.58×109PIB·mL-1w所对应的半致死时间(LT50)分别为8.55d,6.89d,5.9d,4.65d,4.08d。随着浓度的增大,LT50逐渐缩短,说明该病毒的毒力较强,作为杀虫剂具有很好的应用潜力。     

Nutrient element contents of cutting seedlings of hybrid species （ Liriodendron chinense x tulipifera）

The cutting seedlings ofLiriodendron chinense xtulipifera were treated with the different concentrations of auxin (treatment₁: IBA of 50 g·kg⁻¹+NAA of 300 g·kg⁻¹; treatment₂; IBA of 100 g·kg⁻¹+NAA of 300 g·kg⁻¹). The biomass nutrient element contents for different organs (root, stem, leaf) of cutting seedling ofLiriodendron chinense xtulipifera were measured by the dry method, Kjeldahl method and Atomic Absorption Spectroscopy method. The result showed that the biomass of root, stem, and leaf of the cutting seedling treated with auxin was all remarkably increased. The contents of element C in root, stem and leaf had no significant difference between the control and auxin treatments, while the contents of N, P, K and Ca in stem were much lower than that in leaf and root. Variance analysis showed that for the same organ with different concentration treatment of auxin, the four nutrient elements (N, P, K, and Ca) had no significant difference in contents, while there existed significant or very significant difference in contents of the four nutrient elements in different organs with the same concentration auxin treatment. The N, P, K and Ca contents were very low in cutting seedlings; as a result, additional fertilizer should be applied to the seedlings when they were planted in the field. Foundation item: This paper was supported by Jiangsu Province Science Foundation (BE96350). Biography: ZHANG Xiao-ping, (1972-), female, Ph. Doctor in Nanjing Forestry University. Nanjing 210037, P. R. China. Responsible editor: Zhu Hong     

Establishment of cell suspension line of <Emphasis Type="Italic">Populus tomentosa</Emphasis> Carr.

Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L^-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L^-1 2,4-D. The best subculture medium was MS ＋ 0.8 mg＇L-1 2,4-D ＋ 30 g·L^-1 sucrose with a subculture cycle of seven days.     

Dissolved organic carbon and nitrogen in precipitation, throughfall and stemflow fromSchima superba andCunninghamia lanceolata plantations in subtropical China

Despite growing attention to the role of dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) in forest nutrient cycling, their monthly concentration dynamics in forest ecosystems, especially in subtropical forests only were little known. The goal of this study is to measure the concentrations and monthly dynamics of DOC and DON in precipitation, throughfall and stemflow for two plantations ofSchima superba (SS) and Chinese fir (Cunninghamia lanceolata, CF) in Jianou, Fujian, China. Samples of precipitation, throughfall and stemflow were collected on a rain event base from January 2002 to December 2002. Upon collection, all water samples were analyzed for DOC, NO₃ ⁻−N, NH₄ ⁺−N and total dissolved N (TDN). DON was calculated by subtracting NO₃ ⁻−N and NH₄ ⁺−N from TDN. The results showed that the precipitation had a mean DOC concentration of 1.7 mg·L⁻¹ and DON concentration of 0.13 mg·L⁻¹. The mean DOC and DON concentrations in throughfall were 11.2 and 0.24 mg·L⁻¹ in the SS and 10.3 and 0.19 mg·L⁻¹ in the CF respectively. Stemflow DOC and DON concentrations in the CF (19.1 and 0.66 mg·L⁻¹ respectively) were significantly higher than those in the SS (17.6 and 0.48 mg·L⁻¹ respectively). No clear monthly variation in precipitation DOC concentration was found in our study, while DON concentration in precipitation tended to be higher in summer or autumn. The monthly variations of DON concentrations were very similar in throughfall and stemflow at both forests, showing an increase at the beginning of the rainy season in March. In contrast, monthly changes of the DOC concentrations in throughfall of the SS and CF were different to those in stemflow. Throughfall DOC concentrations were higher from February to April, while relatively higher DOC concentrations in stemflow were found during September–November period. Foundation item: This study was supported by the Teaching and Research Award program for MOE P.R.C. (TRAPOYT). Biography: Guo Jian-fen (1977-), female, Ph. Doctor in College of Life Science, Xiamen University, Xiamen 361005, P.R. China. Responsible editor: Zhu Hong     

Optimizing SSR-PCR system of Panax ginseng by orthogonal design

An orthogonal design was used to optimize SSR-PCR amplification system using Panax ginseng genomic DNA as template. Four levels of five factors （DNA template, Taq DNA polymerase, Mg^2＋, primer, and dNTP） and annealing temperature have been tested separately in this system. The results demonstrated the reaction efficiency was affected by these factors. Based on the results, a stable, productive and reproducible PCR system and cycling program for amplifying a ginseng SSR locus were obtained： 20 μL system containing 1.0 U Taq DNA polymerase, 2.0 mmol·L^-1 Mg^2＋, 0.2 mmol·L^-1 dNTPs, 0.3 μmol·L^-1 SSR primer, 60 ng· μla^-1 DNA template, performed with a program of 94℃ for 5 min, 94℃ for 30 s, annealing at 56.3℃ for 30 s, 72℃ for 1 min, 37 cycles, finishing at 72℃ for 7 min, and storing at 4℃.     

Contamination and browning in tissue culture of Platanus occidentalis L.

Twigs of 2–3-year-old Platanus occidentalis L. were used as experimental material to find the causes for the contamination and browning in the initial stages of tissue cultures. To compare the degree of browning of explants picked off from different growing seasons, the experimental material was excised from trees on each of the first ten days in January, March, May and July, 2006. The results indicated that the contamination and browning rates of the material cut off in January (14.2% and 30.6%, respectively) and March were somewhat lower than those in July. The pretreatment of soaking the explants in different anti-oxidants and absorbents at the same time could diminish some side effects. The pretreatment of using 10 g·L–1 vitamin C reduced the contamination and brown-ing rate effectively. An orthogonal experiment showed that the optimal factor and level arrangement is 0.5 mg·L–1 BA, 2.0 g·L–1 ac-tive carbon and 1.5 g·L–1 PVP which resulted in a browning rate of only 16.5%. In general, sampling period, physical properties and pretreatment of explants are the main factors responsible for the contamination and browning of material in the initial stages of P. occidentalis tissue cultures.     

Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

The effects of Timentin and cefotaxime (Cef) on shoot regeneration of the London plane tree (Platanus acerifolia Willd.) and their use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L–1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L–1; however, 300 mg·L–1 Timentin was shown to facilitate shoot r...     

In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f.

In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP (2.0 mg·L^–1) and NAA (0.1 mg·L^–1). The optimal multiplication medium was a modified MS medium supplemented with BAP (1.0 mg·L^–1) and NAA (0.1 mg·L^–1). This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA (0.2 mg·L^–1) and acti-vated charcoal (0.5 mg·L^–1). Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproduci-ble procedure can be adopted for large-scale propagation of T. wilfordii.     

Distribution of gymnemic acid in various organs of Gymnema sylvestre

The gymnemic acid contents in various organs of Gymnema sylvestre were investigated by High Performance Liquid Chromatography (HPLC) method. The results shows that the content of gymnemic acid in various organs, obviously different, was 54.29, 31.66, 28.82, 27.67, 25.39, 20.56 and 1.31 mg·g⁻¹ DW in shoot tips, flowers, nodes, leaves, internodes, roots and seeds, respectively. The highest gymnemic acid content (54.29 mg·g⁻¹ DW) was found in shoot tip, 1.96 fold higher than that in leaves (27.67 mg·g⁻¹ DW). Maximum quantity of gymnemic acid (35.39 mg·g⁻¹ DW) was observed in the young leaves, which was 1.52 times higher than that in old leaves (23.07 mg·g⁻¹ DW). The content of gymnemic acid in young, middle and old internodes was 26.47, 25.77 and 23.94 mg·g⁻¹ DW, respectively, all lower than that in leaves (27.67 mg·g⁻¹ DW), whereas the content of gymnemic acid in young, middle and old nodes was 27.96, 28.81 and 29.66 mg·g⁻¹ DW, respectively, all higher than that in leaves. The study provides the scientific evidences for the rational development and utilization of Gymnema sylvestre resources, since over exploitation of natural stands has caused depletion of these plants in nature. Foundation project: This work was supported by University Grants Commission, New Delhi (No. 33-88/2007 (SR))     

Soil animals and nitrogen mineralization under sand-fixation planta- tions in Zhanggutai region, China

The effects of soil animals on soil nitrogen （N） mineralization and its availability were studied by investigating soil animal groups and their amounts of macro-faunas sorted by hand, and middle and microfaunas distinguished with Tullgren and Baermann methods under three Pinus sylvestris var. mongolica Litv. plantations in Zhanggutai sandy land, China. In addition, soil N mineralization rate was also measured with PVC closed-top tube in situ incubation method. The soil animals collected during growing season belonged to 13 orders, 5 groups, 4 phyla, whose average density was 86 249.17 individuals-m^-2. There were significant differences in soil animal species, densities, diversities and evenness among three plantations. Permanent grazing resulted in decrease of soil animal species and diversity. The average ammonification, nitrification and mineralization rates were 0.48 g：m^-2·a^-1, 3.68 g·m^-2·a^-1 and 4.16 g·m^-2·a^-1, respectively. The ammonification rate in near-mature forest was higher than that in middle-age forests, while the order of nitrification and net mineralization rates was： middle-age forest without grazing 〈 middle-age forest with grazing 〈 near-mature forest with grazing （P〈0.05）. Soil N mineralization rate increased with soil animal amounts, but no significant relationship with diversity. The contribution of soil animals to N mineralization was different for different ecosystems due to influences of complex factors including grazing, soil characteristics, the quality and amount of litter on N mineralization.     

造纸废渣和养分的改良对北部硬阔叶林土壤表面CO2通量的影响

Safe and economical disposal of paper mill sludge is a key consideration for forest products industry. A study was conducted to examine the effects of amendments of sludge and nutrients on soil surface CO₂ flux (R_s) in northern hardwood forests and to quantify the relat among R₅, soil temperature, and moisture in these stands. The experiment was a randomized complete block design that included sludge-amended, fertilized, and control treatments in sugar maple (Acer saccharum Marsh) dominated hardwood forests in the Upper Peninsula of Michigan, USA. Results showed that R_s was positively correlated to soil temperature (R²=0.80, p<0.001), but was poorly correlated to soil moisture. Soil moisture positively affected the R_s only in the sludge-amended treatment. The R_s was significantly greater in the sludge-amended treatment than in the fertilized (p=0.033) and the control (p=0.048) treatments. The maximum R_s in the sludge-amended treatment was 8.8 μmol CO₂·m⁻²·s⁻¹, 91% and 126% greater than those in the fertilized (4.6 μmol CO₂·m⁻²·s⁻¹) and control (3.9 μmol CO₂·m⁻²·s⁻¹) treatments, respectively. The R_s did not differ significantly between the fertilized and control treatments. The difference in R_s between sludge-amended and the other treatments decreased with time following treatment. Foundation item: The research was funded by a NCASI grant to S.T. Gower. Wang CK was supported by Innovated Talent Program of Northeast Forestry University (2004–07) Biography: WANG Chuan-kuan (1963-), male, Professor in the Ecology Program, College of Forestry, Northeast Forestry University, Harbin 150040, China. Responsible editor: Chai Ruihai