Contamination and browning in tissue culture of Platanus occidentalis L. |
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作者单位: | Tao Feng-jie Zhang Zhi-yi Zhou Jun Yao Na Wang Dong-mei Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,100083,P. R. China |
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基金项目: | This study was supported by the Beijing Forestry University Master Candidate Independent Research Fund (05jj037). |
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摘 要: | Twigs of 2–3-year-old Platanus occidentalis L. were used as experimental material to find the causes for the contamination and browning in the initial stages of tissue cultures. To compare the degree of browning of explants picked off from different growing seasons, the experimental material was excised from trees on each of the first ten days in January, March, May and July, 2006. The results indicated that the contamination and browning rates of the material cut off in January (14.2% and 30.6%, respectively) and March were somewhat lower than those in July. The pretreatment of soaking the explants in different anti-oxidants and absorbents at the same time could diminish some side effects. The pretreatment of using 10 g·L–1 vitamin C reduced the contamination and brown-ing rate effectively. An orthogonal experiment showed that the optimal factor and level arrangement is 0.5 mg·L–1 BA, 2.0 g·L–1 ac-tive carbon and 1.5 g·L–1 PVP which resulted in a browning rate of only 16.5%. In general, sampling period, physical properties and pretreatment of explants are the main factors responsible for the contamination and browning of material in the initial stages of P. occidentalis tissue cultures.
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关 键 词: | 法国梧桐 组织培养 棕色着色剂 种植技术 |
收稿时间: | 2006-12-12 |
修稿时间: | 2007-06-23 |
Contamination and browning in tissue culture of <Emphasis Type="Italic">Platanus occidentalis</Emphasis> L. |
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Authors: | Tao Feng-jie Zhang Zhi-yi Zhou Jun Yao Na Wang Dong-mei |
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Institution: | Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education, Beijing Forestry University, 100083,P. R. China |
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Abstract: | Twigs of 2–3-year-old Platanus occidentalis L. were used as experimental material to find the causes for the contamination and browning in the initial stages of tissue
cultures. To compare the degree of browning of explants picked off from different growing seasons, the experimental material
was excised from trees on each of the first ten days in January, March, May and July, 2006. The results indicated that the
contamination and browning rates of the material cut off in January (14.2% and 30.6%, respectively) and March were somewhat
lower than those in July. The pretreatment of soaking the explants in different anti-oxidants and absorbents at the same time
could diminish some side effects. The pretreatment of using 10 g·L−1 vitamin C reduced the contamination and browning rate effectively. An orthogonal experiment showed that the optimal factor
and level arrangement is 0.5 mg·L−1 BA, 2.0 g·L−1 active carbon and 1.5 g·L−1 PVP which resulted in a browning rate of only 16.5%. In general, sampling period, physical properties and pretreatment of
explants are the main factors responsible for the contamination and browning of material in the initial stages of P. occidentalis tissue cultures. |
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Keywords: | Platanus occidentalis L tissue culture contamination browning |
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