2. Both methionine sources were added at equimolar concentrations of 0.03, 0.07, 0.11, 0.15, 0.20 and 0.25% to the diet.
3. A non‐linear multi‐exponential regression model was used to describe the weight gain and food conversion response to supplemental sources of methionine.
4. Compared with DL‐methionine on an equimolar basis, bioefficacy of MHA‐FA was calculated at 73% from weight gain and 68% from food conversion data, respectively. This corresponds to an efficiency of Alimet® between 60 and 64% relative to DL‐methionine on a weight for weight basis. 相似文献
2. In a growth assay, finishing broilers 21 to 42 d of age were fed on diets containing graded amounts of the two supplements; the basal diet was composed of practical ingredients.
3. From slope‐ratio analysis, equimolar efficacy of MHA‐FA relative to DL‐methionine was determined to be 83% (confidence limits 61 to 115%) from weight gain responses, and 67% (47 to 91%) from food conversion responses. This indicates that the relative efficacy of MHA‐FA is close to previous estimates of about 75% obtained under thermoneutral conditions.
4. In a balance study with caecectomised cockerels, net absorption (intake—excretion in faeces and urine) of DL‐methionine and of MHA‐FA, respectively, were determined to be 97.2 and 90.8%. The net absorption of MHA‐FA was significantly lower than that of DL‐methionine. 相似文献
2. Egg production was significantly improved by feeding diets containing 120 or 140 g GP/kg compared with 100 g/kg, and egg weight was significantly improved by feeding 140 g CP/kg compared with 120 g/kg.
3. These results indicate that an amino acid(s) other than MET was limiting in diets from which hens obtained 16 g CP, 0.3 g MET and 0.6 g total sulphur amino acids (TSAA)/bird d, and that MET was not limiting at 24 g CP, 0.4 g MET and 0.8 g TSAA/bird d. 相似文献
2. Supplementing the diet with DAC equivalent to 25 g protein/kg did not improve egg production, the efficiency of food utilisation, egg weight, nitrogen retention or the apparent absorption of lysine and methionine; increases in food intake and in the concentration of methionine in the serum and liver were observed.
3. Adding sodium sulphate, alone or with DAC, did not affect the variables noted above.
4. Supplementation of the basal diet with methionine increased egg production, egg weight, food intake and the concentrations of lysine in the serum and liver.
5. It is concluded that the supplemental NPN was used only in serum protein synthesis. 相似文献
2. Kidney damage was not observed in pullets reared on NC diets. For pullets fed on HC diets, strain A developed significantly greater kidney asymmetry, a higher incidence of gross kidney damage and a higher incidence of uroliths than strain B.
3. Supplementing the HC diet with 6 g/kg DL‐methionine significantly reduced the incidence of calcium‐induced gross kidney damage and urolith formation in both strains. Ammonium sulphate (5.3 g/kg) was significantly more effective than dl‐methionine in reducing calcium‐induced kidney damage.
4. Neither dl‐methionine nor ammonium sulphate caused a measurable metabolic acidosis. Neither supplement consistently affected water consumption or manure moisture. 相似文献
2. In experiment 1, 5 graded amounts of a DL‐methionine and L‐cysteine (1:1 by weight) mixture were added to basal diets containing 197 or 233 g crude protein/kg. The diets containing 197 g protein/kg were fed with or without the further addition of 36 g crude protein/kg from nonessential amino acids. The amino acid balance of all diets was kept constant for all essential amino acids except the SAA. In experiment 2, 5 graded amounts of SAA from either a crystalline source (DL‐methionine or a mixture of DL‐methionine and L‐cysteine) or from intact proteins were added to a diet containing 208 g protein/kg.
3. At each protein concentration there were significant responses to the SAA addition in weight gain, food conversion efficiency, and carcase quality. Non‐linear exponential regression analyses were used to describe bird responses to SAA concentration. The broiler chick's requirement for SAA increased with increasing dietary protein concentrations ranging from 197 to 259 g protein/kg.
4. The utilisation of SAA differed also with differences in origin (crystalline or peptide‐bound), and methionine:cysteine balances. Compared to DL‐methionine, a 1:1 mixture of DL‐methionine and L‐cysteine was only 81% or 86% as effective in supporting growth or food conversion, respectively. SAA from added protein was even less effectively utilised.
5. The addition of nonessential amino acids tended to decrease food intake without affecting SAA utilisation.
6. Slaughter yield and breast meat yield were clearly increased while fat deposition was clearly decreased, by SAA addition. The response in breast meat yield suggested an important economic benefit for further meat processing. Nitrogen retention was significantly enhanced by SAA supplementation from crystalline sources, and this led to reductions of up to 30% in the amount of nitrogen excreted per kg weight gain. 相似文献
2. Deposition of 14C label into breast and heart muscles responded positively to dietary lysine concentration. There was no apparent relationship between dietary lysine intake and deposition of label into carbon dioxide or excreta.
3. Efficiency of lysine retention, above maintenance, was estimated to be 0.523 ± 0.034.
4. Turkeys exhibited variable metabolic efficiency in the use of lysine as indicated by loss of U‐14C‐lysine label to expired carbon dioxide and to excreta by individual turkeys. 相似文献
2. The microbiological condition of the carcasses was determined initially and after storage at — 2 oC for 28, 35 and 42 d. It was found that, whilst pseudomonads (pigmented and non‐pigmented) were present at 108/cm2 after 35 and 42‐d storage, yeasts were also present at 107/cm2 and probably accounted for the unusual fusty “off” odours. 相似文献
Background
The ACTH stimulation test is currently required for definitive diagnosis of hypoadrenocorticism. Increased cost of synthetic ACTH (cosyntropin) has prompted a search for alternative diagnostic methods.Objective
The purpose of this study was to determine whether a cortisol‐to‐ACTH ratio (CAR) can be used to differentiate dogs with hypoadrenocorticism from normal dogs and those with nonadrenal illness.Animals
Eight healthy dogs (H), 19 dogs with nonadrenal illness (NAI), and 15 dogs with hypoadrenocorticism (HAD).Methods
Dogs in the HAD group were retrospectively identified from PUVTH medical records. The NAI group consisted of hospitalized dogs with clinical signs, clinicopathologic findings, or both, consistent with a diagnosis of hypoadrenocorticism, but in which hypoadrenocorticism was ruled out based on ACTH stimulation test results. Healthy dogs were recruited from hospital staff and students. Endogenous ACTH concentrations and cortisol concentrations before and after ACTH stimulation were measured in all dogs.Results
Baseline cortisol concentration was significantly lower, and ACTH concentration was significantly higher, in the HAD group versus the H and NAI group (P < .001). However, there was overlap among groups. Cortisol‐to‐ACTH ratio was significantly lower in the HAD group versus the H and NAI groups (P < .001), and there was no overlap between the HAD group and the other 2 groups.Conclusions and Clinical Importance
CAR can be used for definitive diagnosis of primary hypoadrenocorticism. 相似文献2. The relationships between incidence of down‐grading and age, body weight, sex, size of rearing site and its distance from the processing plant, and various weather conditions during the rearing period of each flock were examined using regression analysis.
3. Age and body weight and rearing site size were the only factors significantly correlated with amount of blistering. 相似文献
2. Irrespective of age, reserpinised chicks became hyperglycaemic and hypocholesteraemic, their stores of adrenal cholesterol were depleted and the mobilisation of the lipid was impaired. The response of the older bird was quantitatively larger than that of the younger bird.
3. Propranolol prevented the increase in plasma glucose concentration seen 1 h after reserpine but it did not affect subsequent changes.
4. It is concluded that reserpine acts as a stressor and it is shown that its effects have not been entirely overcome after 24 h. 相似文献
2. Changes in plasma cholesterol were equivocal, though there was perhaps a trend towards hypercholesteraemia.
3. Birds became hypoglycaemic within an hour but were hyper‐glycaemic between 2 and 4 h. At 24 h they were normoglycaemic.
4. Plasma corticosterone was increased markedly at 1 h but was decreased compared with the control at 2 h (P<0.01). Thereafter the concentration was in the normal range.
5. There was an enhanced hyperlipacidaemia in the treated birds after 1 h.
6. Adrenal cholesterol stores were decreased by 10% at 4 h but were unaffected at all other times.
7. Colonic temperature decreased by 1–6 °C after 2 h but was normal by4h.
8. It is concluded that propranolol has mild stressor activity which is lost within 24 h. 相似文献
2. Crambe meals were prepared to differ in E‐PG (19, 36 and 40 g/kg) and CHB contents (0.1, 0.7 and 1.9 g/kg), and with either active or inactive thioglucosidase.
3. Meals were fed to 7‐d‐old broiler chicks at 50 or 100 g/kg of the diet for 12 or 13 d. In separate studies, isolated E‐PG or CHB were mixed into the diet or administered by gavage to 7‐d‐old broiler chicks in amounts equivalent to 50 or 100 g/kg crambe meal diets for 10 and 12 d, respectively.
4. Weight gain decreased (P<0.05) in chicks fed on the high glucosinolate crambe diets or isolated E‐PG. Food consumption decreased (P<0.05) in chicks fed on the diet containing the high E‐PG meal with active enzyme.
5. Mild liver lesions and increased serum aspartate aminotransferase were found in chicks fed on the diet containing the high glucosinolate meal with active enzyme. Other organs, including thyroids, were normal.
6. Commercially‐processed crambe meal appeared safe at an inclusion rate of 50 or 100 g/kg diet, but could not be recommended at this point for long term feeding. 相似文献
The results indicate that with certain breeds the dietary protein levels can be lowered to approximately 16% during the o to 8‐week period and to approximately 12% during the 8 to 20‐week period without adversely affecting egg production. However, variations in the laying performance of the different breeds appear to be dependent on the amount of protein fed in the first eight weeks of life. Significant breed effects were observed throughout the experiment and although restricted feeding during the laying period substantially reduced the food intake it also had a detrimental effect on the rate of egg production and on the total weight of eggs produced. 相似文献
Background
Canine cutaneous T‐cell lymphoma (CTCL) is an uncommon disease for which efficacious therapies are lacking. The novel anticancer nucleotide prodrug VDC‐1101 (formerly known as GS‐9219) has shown efficacy in dogs with multicentric lymphoma. One of the observed adverse effects with this drug was a skin change characterized by hair loss, erythema, and pruritus, implying delivery of VDC‐1101 to the skin.Hypothesis/Objectives
The primary study objective was to identify the objective response rate (ORR) to VDC‐1101 in canine CTCL; secondary objectives included characterization of progression‐free survival (PFS) and adverse events (AEs).Animals
Twelve dogs with chemotherapy‐naïve or relapsed, histologically and immunohistochemically confirmed CTCL.Methods
Dogs received VDC‐1101 as a 30‐minute IV infusion once every 21 days. Prednisone (1 mg/kg PO q48h) was administered concurrently.Results
In 11 evaluable patients, responses included 1 complete response (CR), 4 partial responses (PR), 2 stable disease (SD), and 4 progressive disease for an ORR of 45% and biologic response rate (CR/PR/SD) of 64%. The median PFS was 37.5 days (26 to >399 days), which includes 1 durable and ongoing CR (>1 year). Gastrointestinal and hematologic AEs were mild; no dogs developed grade 3 or 4 AEs. Three dogs developed dermatopathies and 1 of these dogs was removed from the study as a result of this AE.Conclusions and Clinical Importance
VDC‐1101 has activity against canine CTCL and could provide another treatment option in a disease process with a poor prognosis. 相似文献2. In order to evaluate the role of type X collagen cross‐linking on eggshell formation, hens were injected with different doses of β‐aminoproprionitrile, which specifically interferes with cross‐link formation.
3. Changes in egg size and shape were observed. Scanning electron micrographs analysis of these eggs demonstrated marked changes in crystal growth and shell membrane structure and arrangement. A dot‐blot analysis, using a monoclonal antibody against chicken type X collagen, shows a dose‐dependent increase in shell membrane collagen extractability.
4. It is concluded that the formation of β‐aminoproprionitrile‐sensitive cross‐links among the type X collagen molecules of the shell membranes play an essential role in normal eggshell formation. 相似文献
2. Doubling the D3 supplement in the control diet (27.5 μg or 1100 IU/kg) almost linearly increased the circulating concentration of 25‐OH‐D3 without raising the concentration of calcitriol, Ca, or egg SG.
3. Replacing D3 by the optimal concentration of calcitriol (5 μg/kg diet) improved egg SG after 21 weeks of treatment without increasing blood calcitriol or total Ca.
4. By itself, 24,25‐dihydroxycholecalciferol [24,25‐(OH)2D3] was unable to maintain normal blood levels of calcitriol, Ca or egg SG and, when added together with calcitriol in the diet, tended to elevate blood Ca but suppress the beneficial effect of calcitriol on shell quality, with little or no effect on blood calcitriol. 相似文献