Background
E‐point‐to‐septal‐separation (EPSS) and the sphericity index (SI) are echocardiographic parameters that are recommended in the ESVC‐DCM guidelines. However, SI cutoff values to diagnose dilated cardiomyopathy (DCM) have never been evaluated.Objectives
To establish reference ranges, calculate cutoff values, and assess the clinical value of SI and EPSS to diagnose DCM in Doberman Pinschers.Animals
One hundred seventy‐nine client‐owned Doberman Pinschers.Methods
Three groups were formed in this prospective longitudinal study according to established Holter and echocardiographic criteria using the Simpson method of disk (SMOD): control group (97 dogs), DCM with echocardiographic changes (75 dogs) and “last normal” group (n = 7), which included dogs that developed DCM within 1.5 years, but were still normal at this time point. In a substudy, dogs with early DCM based upon SMOD values above the reference range but still normal M‐Mode measurements were selected, to evaluate if EPSS or SI were abnormal using the established cutoff values.Results
ROC‐curve analysis determined <1.65 for the SI (sensitivity 86.8%; specificity 87.6%) and >6.5 mm for EPSS (sensitivity 100%; specificity 99.0%) as optimal cutoff values to diagnose DCM. Both parameters were significantly different between the control group and the DCM group (P < 0.001), but were not abnormal in the “last normal” group. In the substudy, EPSS was abnormal in 13/13 dogs and SI in 2/13 dogs.Conclusions and Clinical Importance
E‐point‐to‐septal‐separation is a valuable additional parameter for the diagnosis of DCM, which can enhance diagnostic capabilities of M‐Mode and which performs similar as well as SMOD. 相似文献Background
Spec cPL is the most sensitive and specific test for diagnosing pancreatitis in dogs. Its results have not been compared to those of the 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6′‐methylresorufin) ester (DGGR) lipase assay or those of abdominal ultrasonography.Objectives
To investigate agreement of Spec cPL with DGGR lipase activity and pancreatic ultrasonography in dogs with suspected pancreatitis.Animals
One hundred and forty‐two dogs.Methods
DGGR lipase activity (reference range, 24–108 U/L) and Spec cPL were measured using the same sample. The time interval between ultrasonography and lipase determinations was <24 hours. The agreement of the 2 lipase assays at different cutoffs and the agreement between pancreatic ultrasonography and the 2 tests were assessed using Cohen''s kappa coefficient (κ).Results
DGGR lipase (>108, >216 U/L) and Spec cPL (>200 μg/L) had κ values of 0.79 (95% confidence interval [CI], 0.69–0.9) and 0.70 (CI, 0.58–0.82). DGGR lipase (>108, >216 U/L) and Spec cPL (>400 μg/L) had κ values of 0.55 (CI, 0.43–0.67) and κ of 0.80 (CI, 0.71–0.9). An ultrasonographic diagnosis of pancreatitis and DGGR lipase (>108, >216 U/L) had κ values of 0.29 (CI, 0.14–0.44) and 0.35 (CI, 0.18–0.52). Ultrasonographically diagnosed pancreatitis and Spec cPL (>200, >400 μg/L) had κ values of 0.25 (CI, 0.08–0.41) and 0.27 (CI, 0.09–0.45).Conclusions and Clinical Importance
Although both lipase assays showed high agreement, agreement between ultrasonography and lipase assays results was only fair. Because lipase results are deemed more accurate, ultrasonography results should be interpreted carefully. 相似文献2. Crambe meals were prepared to differ in E‐PG (19, 36 and 40 g/kg) and CHB contents (0.1, 0.7 and 1.9 g/kg), and with either active or inactive thioglucosidase.
3. Meals were fed to 7‐d‐old broiler chicks at 50 or 100 g/kg of the diet for 12 or 13 d. In separate studies, isolated E‐PG or CHB were mixed into the diet or administered by gavage to 7‐d‐old broiler chicks in amounts equivalent to 50 or 100 g/kg crambe meal diets for 10 and 12 d, respectively.
4. Weight gain decreased (P<0.05) in chicks fed on the high glucosinolate crambe diets or isolated E‐PG. Food consumption decreased (P<0.05) in chicks fed on the diet containing the high E‐PG meal with active enzyme.
5. Mild liver lesions and increased serum aspartate aminotransferase were found in chicks fed on the diet containing the high glucosinolate meal with active enzyme. Other organs, including thyroids, were normal.
6. Commercially‐processed crambe meal appeared safe at an inclusion rate of 50 or 100 g/kg diet, but could not be recommended at this point for long term feeding. 相似文献
2. Efficiency of food utilisation, protein efficiency ratio and dressing percentage indicated that substitution of FM or GC by alga did not affect the performance of broilers.
3. None of the diets affected the weights, compositions and histopathology of the various organs of the chicks.
4. Meat quality remained unchanged except for a more intense colour in the case of birds fed on the alga‐containing diets. 相似文献
2. GH concentrations were higher in restricted compared with ad libitum‐fed birds at 17 to 21 weeks of age and were similar thereafter. Plasma IGF‐I concentrations were higher in restricted compared with ad libitum‐fed birds throughout the sampling period of the experiment.
3. The age at first egg was delayed by about two weeks in restricted birds. At 54 weeks of age they had laid 114 compared with 67 eggs in birds fed ad libitum throughout and had produced less than half the number of eggs with defective shells.
4. It was suggested that differences in egg production between ad libitum and restricted broiler breeder females could be related to changes in the pattern of GH and/or IGF‐I secretion. 相似文献
2. Body weight, sexual maturity, egg weight, the number of abnormal and cracked eggs, efficiency of food utilisation and mortality were not affected either by lighting treatment or by genotype.
3. The genotype X treatment interaction was highly significant for egg number, laying percentage and clutch length to 362 d. The S females were not affected by light intensity. Albino hens of “high” light intensity were superior to the other 3 groups, laying 12 per cent more eggs than non‐albino hens; but their egg production was moderately depressed by “dim” light relative to others. 相似文献
2. The laying rate of albinos was higher than that of nonalbinos before the forced moult but not afterwards. Hierarchical follicles in albino hens were smaller than those of nonalbinos before but not after the moult.
3. Growth intensity of hierarchical follicles was greater in albino hens before the moult, suggesting that follicular maturation was more rapid, possibly explaining the higher egg production observed.
4. All differences between genotypes disappeared after the moult, suggesting that the state of the ovary before a moult is independent of that afterwards. 相似文献
2. This finding prompted an experiment in which plasma LH concentrations were measured between 12 and 33 weeks of age when daylength was increased at 15 or 21 weeks. Egg production of the early and late maturing albino hens was measured.
3. Plasma LH concentrations overall and at 17 weeks were lower for albinos than for nonalbinos. In the early maturing group egg production of albinos was higher than that of nonalbinos.
4. Results suggest that increased egg production of albinos is not the direct result of differences in plasma LH concentrations but may be a consequence of differences in the control of LH secretion. 相似文献
Background
Systemic oxidative stress in horses with recurrent airway obstruction (RAO) is poorly characterized.Objectives
The goal of this study was to investigate whether equine RAO is associated with systemic disturbances in the oxidant‐antioxidant equilibrium.Animals
Seven healthy horses and 7 horses with symptomatic RAO.Methods
A prospective study. Healthy and RAO‐affected horses were exposed to a 48‐hour challenge with moldy hay and straw to induce clinical exacerbation of RAO. Venous blood was collected and the activities of the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in equine erythrocyte lysates were measured. The concentration of thiobarbituric acid‐reactive substances (TBARSs) was assessed both in erythrocyte lysates and in plasma.Results
A significant increase in the activities of GPx and SOD was detected in RAO‐affected horses compared with the control animals. There was no significant difference between groups in terms of the erythrocyte lysate activities of CAT, GR, or TBARs or the plasma concentration of TBARs.Conclusion and Clinical Importance
Our results support the hypothesis that RAO in horses is associated with systemic oxidative stress. Future studies are needed to assess whether horses suffering from RAO can benefit from antioxidant supplementation. 相似文献Background
Steroid‐responsive meningitis‐arteritis (SRMA) is an inflammatory disease of dogs that is suspected to be immune‐mediated. The development of other immune‐mediated diseases has been linked to vaccinations, time of the year, geographic location, sex, neuter status, and breed.Hypothesis/Objectives
To identify if the development of SRMA is associated with time of year, vaccination, geographic location, sex, neuter status, and breed.Animals
Sixty SRMA cases and 180 controls, all ≤24 months of age and matched for year of presentation, from a referral hospital population in the United Kingdom.Methods
Retrospective case‐control study with unconditional logistic regression analysis.Results
Beagles (P = .001), Border Collies (P = .001), Boxers (P = .032), Jack Russell Terriers (P = .001), Weimaraners (P = .048), and Whippets (P < .001) had significantly greater odds of developing SRMA in this population of dogs. Vaccination, time of year, geographic category, sex, and neuter status did not increase the odds of developing SRMA.Conclusions and Clinical Importance
Only breed increased the odds of developing SRMA. It would be prudent to investigate the genetics of the identified breeds to help elucidate the etiopathogenesis of SRMA. 相似文献2. The caeca open ventro‐laterally from the cranial end of the colon. At this level a muscular ring of tissue, formed from the circular muscle of the lamina muscularis of the ileum, projects into the colon lateral to the caecal openings. The location of this muscular ring is discussed in relation to the possible mechanisms of caecal filling.
3. The longitudinal muscle of the lamina muscularis of the caudal region of each caecum is continuous with that of the colon and ileum. The circular muscle of the lamina muscularis of each caecum begins in the muscularis mucosa of the colon at a distance of 1 to 2 mm from the caecal openings. At the level of the caecal openings the musculature is complex, consisting of parts of the lamina muscularis of the colon, caeca and ileum.
4. The openings of the caeca from the colon are narrow and filled with villi. It is suggested that the junction may be involved in filtering material during the filling of the caeca. 相似文献
2. Live weight and total meat was reduced by 37.4 and 42.8% in dw/ ‐ as compared with Dw+/‐ females at the age of 9 weeks. The suppressive effect of the dwarf allele on the weight of m. pectoralis superficialis was 40.6% (67.8 v. 40.3 g in Dw+ / ‐ and dw/ ‐ genotypes). This reduction in weight was accompanied by a similar decrease of DNA content (28.8 v. 17.1 mg, i.e. 40.6%), a decrease in number of muscle fibres (829 × 103 v. 654 × 103, i.e. 21.2%) and fibre diameter (30.80 v. 28.95 μm, i.e. 6.0%). In mature hens, the differences between genotypes Dw+ / — and dw / ‐ were slightly smaller.
3. The different relationships between the muscle growth traits studied are discussed. 相似文献
Background
Published information regarding survival and long‐term cardiac remodeling after patent ductus arteriosus (PDA) closure in dogs is limited.Objectives
To report outcome and identify prognostic variables in dogs with PDA, and to identify risk factors for persistent remodeling in dogs with a minimum of 12 months of follow‐up after closure.Animals
Five hundred and twenty client‐owned dogs.Methods
Retrospective review of medical records of 520 dogs with PDA. Outcome was determined by contacting owners and veterinarians. Dogs with PDA closure and ≥ 12 months of follow‐up were asked to return for a re‐evaluation.Results
In multivariable analysis of 506 dogs not euthanized at the time of diagnosis, not having a PDA closure procedure negatively affected survival (HzR = 16.9, P < .001). In 444 dogs undergoing successful PDA closure, clinical signs at presentation (HzR = 17, P = .02), concurrent congenital heart disease (HD) (HzR = 4.8, P = .038), and severe mitral regurgitation (MR) documented within 24 hours of closure (HzR = 4.5, P = .028) negatively affected survival. Seventy‐one dogs with ≥ 12 months follow‐up demonstrated a significant reduction in radiographic and echocardiographic measures of heart size (P = 0) and increased incidence of acquired HD (P = .001) at re‐evaluation. Dogs with increased left ventricular size and low fractional shortening at baseline were more likely to have persistent remodeling at re‐evaluation.Conclusions and Clinical Importance
Patent ductus arteriosus closure confers important survival benefits and results in long‐term reverse remodeling in most dogs. Clinical signs at presentation, concurrent congenital HD, and severe MR negatively affect survival. Increased left ventricular systolic dimensions and systolic dysfunction at baseline correlated significantly with persistent remodeling. 相似文献Background
Long‐term microscopic agglutination test (MAT) results after vaccination with 4‐serovar Leptospira vaccines are not available for all vaccines used in client‐owned dogs.Hypothesis/Objectives
To determine antibody responses of client‐owned dogs given 1 of 4 commercially available Leptospira vaccines.Animals
Healthy client‐owned dogs (n = 32) with no history of Leptospira vaccination for at least the previous year.Methods
Dogs were given 1 of 4 Leptospira vaccines on week 0 and then approximately on week 3 and week 52. Sera were collected before vaccine administration on week 0 and then within 3 days of week 3, within 2 days of week 4, and approximately on weeks 7, 15, 29, 52, and 56. Antibody titers against Leptospira serovars bratislava, canicola, grippotyphosa, hardjo, icterohemorrhagiae, and pomona and were determined by MAT.Results
When compared among vaccines, MAT results varied in maximal titers, the serovars inducing maximal titers, and the time required to reach maximal titers. Each vaccine induced at least some MAT titers ≥1 : 800. Most dogs were negative for antibodies against all serovars 1 year after vaccination, and anamnestic responses were variable.Conclusions and Clinical Importance
Dogs vaccinated with Leptospira vaccines have variable MAT titers over time, and antibodies should not be used to predict resistance to Leptospira infection. MAT titers ≥1 : 800 can develop after Leptospira spp. vaccination, which can complicate the clinical diagnosis of leptospirosis. 相似文献Background
Serum N‐terminal pro‐C‐natriuretic peptide (NT‐proCNP) concentration at hospital admission has sufficient sensitivity and specificity to differentiate naturally occurring sepsis from nonseptic systemic inflammatory response syndrome (SIRS). However, little is known about serum NT‐proCNP concentrations in dogs during the course of sepsis.Objective
To determine serum NT‐proCNP and cytokine kinetics in dogs with endotoxemia, a model of canine sepsis.Samples
Eighty canine serum samples.Methods
Eight healthy adult Beagles were randomized to receive Escherichia coli lipopolysaccharide (LPS, 5 μg/kg) or placebo (0.9% NaCl) as a single IV dose in a randomized crossover study. Serum collected at 0, 1, 2, 4, and 24 hours was stored at −80°C for batch analysis. Serum NT‐proCNP was measured by ELISA and 13 cytokines and chemokines by multiplex magnetic bead‐based assay.Results
Serum NT‐proCNP concentrations did not differ significantly between LPS‐ and placebo‐treated dogs at any time. When comparing serum cytokine concentrations, LPS‐treated dogs had higher interleukin‐6 (IL‐6), IL‐10, TNF‐α and KC‐like at 1, 2, and 4 hours; higher CCL2 at 1, 2, 4, and 24 hours; and higher IL‐8 and CXCL10 at 4 hours compared to placebo‐treated dogs. There were no differences in serum GM‐CSF, IFN‐γ, IL‐2, IL‐7, IL‐15 or IL‐18 between LPS‐ and placebo‐treated dogs.Conclusions and Clinical Importance
Serum NT‐proCNP concentration does not change significantly in response to LPS administration in healthy dogs. Certain serum cytokine and chemokine concentrations are significantly increased within 1–4 hours after LPS administration and warrant further investigation as tools for the detection and management of sepsis in dogs. 相似文献2. Hens identified as broody and removed from the flock had higher plasma prolactin concentrations than the laying hens at each of the three experimental stages.
3. Confinement in sand and wire floored pens, induced a decline in plasma prolactin concentrations. This decline probably impeded immediate development of broody behaviour. Alternately, levels of prolactin higher than those of laying hens were again measured 7 and 14 days after treatment during third week but not after the 10 th and 16th week of production.
4. Confinement did not induce consistent changes in luteinising hormone (LH) and progesterone concentrations from one period to an other.
5. An increase in the plasma concentration of D‐(/?)‐hydroxybutyrate was observed in the hens which had an egg present in the oviduct on day 2, 3 and/or 4 of the treatment. Subsequently, a decrease in ovulation rate was observed in the hens with higher concentrations of D‐(β)‐hydroxybutyrate while under treatment, during the 10th week of production.
6. These data confirm that the effectiveness of the traditional methods for broodiness prevention under commercial conditions is related to the induction of a decrease of prolactin. 相似文献