纯中药"孕宝"冲剂治疗母牛子宫内膜炎性不孕症的疗效观察

为了探讨中药“孕宝”治疗母牛子宫内膜炎性不孕症的临床疗效，本试验积累选择子宫内膜炎性不孕病牛４９７０头，随机分为两组，其中，试验组４９５３头（慢性卡他性３２４２头，隐性５６３头，慢性卡他性脓性１１４８头）；对照组１７头（慢性卡他性９头，隐性３头，慢性卡他性脓性５头）。试验组根据病情的轻重分别投服“孕宝”１～３剂，对照组不用药。结果表明，中药“孕宝”治疗子宫内膜炎病牛的总有效率为９６％。治愈率达９４     

促孕一剂灵中药粉制剂

促孕一剂灵是中药粉制剂,主要成份为益母草、干姜、白芍、红花、黄芩等.经我们试验,对治疗母牛排卵障碍、隐性子宫内膜炎有较好的疗效.该试验是通过应用促孕一剂灵治疗母牛排卵障碍、隐性子宫内膜炎,提高受胎率,缩短母牛空怀时间,增加农民的养牛经济效益.     

不孕症黄牛阴道微生态变化及其在治疗上的应用

以淮北地区黄牛为对象，定量检测了正常繁育母牛和不孕症母牛各１０头的阴道分泌物菌群。正常繁育母牛和不孕症母牛阴道菌群的主要菌类相同。前者需氧菌分离率４０％，平均密度＜１０６／ｇ；厌氧菌分离率８０％，平均密度＞１０８／ｇ；优势菌为乳杆菌。后者需氧菌分离率８０％，平均密度＞１０７／ｇ；厌氧菌分离率５０％，平均密度＜１０６／ｇ；优势菌为表皮葡萄球菌。药物敏感性试验表明，不孕牛阴道优势菌表皮葡萄球菌对氯霉素高度敏感，对新霉素、庆大霉素、链霉素中度敏感，对青霉素等不敏感。根据上述结果，分别应用氯霉素和中药方剂对患有子宫内膜炎的不孕母牛进行了治疗试验，以子宫内膜炎症状消失并受胎为指标，氯霉素治疗的总有效（受胎）率为８８．８％（１９１／２１５），平均用药１．３次；中药方剂的总有效（受胎）率为８５．５％（６５／７６），平均用药次数１．４次。     

清宫促孕灌注液的质量标准及临床试验

为建立清宫促孕灌注液的质量控制方法和评价其对母猪子宫内膜炎的疗效。用分光光度法测定制剂指标成分总黄酮的含量,并以０．１％高锰酸钾溶液和缩宫素合用作对照,观察清宫促孕灌注液治疗母猪子宫内膜炎的临床效果,其结果,线性范围０～４９．９６８ｕｇ,平均回收率１０１．０２％（ｎ＝６）,ＲＳＤ为０．９７％。清宫促孕灌注液对母猪脓性子宫内膜炎的治愈率为９６．２％,疗效优于对照药物,含量测定方法简便、快速、准确、重     

不同药物治疗母猪子宫内膜炎的效果比较

应用促孕灌注液、宫炎清、青、链霉素等药剂进行母猪子宫内膜炎的治疗比较试验。结果表明:宫炎清治疗母猪急性子宫内膜炎和隐性子宫内膜炎的有效率均为100%,治愈率分别为93.1%和83.3%;促孕灌注液对这两种类型子宫内膜炎的治疗有效率为91.7%和85.7%,治愈率分别为79.2%和71.4%。对慢性子宫内膜炎的治疗,促孕灌注液有效率和治愈率分别为86.4%、72.7%,宫炎清为81.8%、72.7%。两种药物对母猪子宫内膜炎的疗效明显高于青、链霉素。     

绒毛膜促性腺激素、促黄体素和促排3号对奶牛卵泡囊肿治疗效果的研究

本试验应用不同投药途径的绒毛膜促性腺激素（ＨＣＧ）、促黄体素（ＬＨ）和促排３号（ＬＲＨ─Ａ３）等激素对１５０例患有卵泡囊肿的奶牛进行了疗效试验，取得了较好的结果。静注ＨＣＧ５０００单位（试验１组）的治愈率为９４％（４９／５２），比肌注ＨＣＧ５０００～１００００单位（试验２组）的治愈率５０％（１５／３０）高４４％（ｐ＜０．０１）；静注ＬＨ２００单位（试验３组）的治愈率为９０％（１８／２０），比肌注ＬＨ２００单位（试验４组）的治愈率５０％（１０／２０）高４０％（ｐ＜０．０１）；卵泡囊肿侧的阴唇粘膜注射ＬＲＨ─Ａ３１０００μｇ（试验６组），其治愈率为８５．７１％（１２／１４），这种方法不仅比每日肌注一次ＬＲＨ─Ａ３１０００μｇ，连续注射２次（试验５组）的治愈率６４．２９％（９／１４）高２１．４２％，而且注射次数和用药量均为肌注法的１／２。     

不同给药途径的15甲基前列腺素F_2α和乙烯雌酚注射液对奶牛持久黄体疗效的比较

本文应用不同给药途径的１５甲基前列腺素Ｆ２α（１５甲基ＰＧＦ２α）和乙烯雌酚注射液对１００例患有持久黄体的奶牛进行疗效的比较，结果表明，有黄体存在的卵巢侧的阴辱粘膜下注射１５甲基ＰＧＦ２α２ｍｇ（试验１组）效果最佳，治愈率为９０％（１８／２２），有效率为１０％（２／２０）；有黄体存在的卵巢侧的子宫角注射１５甲基ＰＧＦ２α２ｍｇ（试验２组），治愈率（１８／２０）和有效率（２／２０）同试验１组；肌注１５甲基ＰＧＦ２α４ｍｇ（试验３组）的治愈率为７０％（１４／２０），有效率为３０％（６／２０）；每日肌注１次乙烯雌酚注射液３５ｍｇ，连续注射２天（试验４组），治愈率为６０％（１２／２０），有效率为４０％（８／２０）；每日在有黄体存在的卵巢侧的阴唇粘膜下注射１次乙烯雌酚注射液３５ｍｇ（试验５组），连续注射２天，治愈率为８０％（１６／２０），有效率为２０％（４／２０）。试验１组、２组与试验３组比，差异极显著（Ｐ＜０．０１）；试验５组与试验４组比，差异亦为极显著（Ｐ＜０．０１）；试验４组与试验３组比和试验５组与试验１、２组比，差异均为不显著（Ｐ＞０．０５）。     

不同给药途径的前列腺素,催产素对治疗奶牛持久黄体效果的研究

本文同给药途径的前列腺素Ｆ２α和催产素７５头患有持久黄体的奶牛进行治疗效果的试验，结果表明，有黄体侧阴唇粘膜下注射ＰＧＦ２α６ｍｇ（试验１组）效果最佳，治愈率为９３．３３％（１４／１５），有效率为６．６７％（１／１５）；子宫角深部注射ＰＧＦ２α６ｍｇ（试验２组）治愈率和有交与试验１组相同；肌注ＰＧＦ２α１２ｍｇ（试验３组）治愈率为７３．３３％（１１／１５），有效率为２６．６７％（４／１５）；每２小     

新一代喹诺酮治疗仔猪白痢试验

将１５５头仔猪白简病猪随机分为２组，试验组１０５头，对照组５０头，分别肌肉注射新一代喹诺酮（ＦＱＳ１１０）与氟哌酸注射液治疗３ｄ，剂量分别为０．５ｍｇ／ｋｇ体重与４．０ｍｇ／ｋｇ体重。结果，试验组治愈１０１头，好转４头，治愈率与有效率分别为９６％与１００％；对照组治愈４５头，好转２头，无效２头，治愈率有效率分别为９０％与９６％。     

奶牛子宫内膜炎非抗菌治疗试验

选用８６头患子宫内膜炎的奶牛分为３组进行非抗菌治疗试验，１，２，３组分别采用催产素，氯前列烯醇和碘溶液治疗。结果表明：１，２，３组的治愈率，好转率和无效率分别为８０，５％，１２．２％，７．３％，８６．４％，９．１％，４．１％，６９．６０％，２１．７％，８．７％。     

多种药物治疗奶牛子宫内膜炎的疗效比较试验

对患有卡他性和脓性子宫内膜炎的病牛采用多种药物进行治疗,对治疗效果进行比较分析。结果表明:治疗卡他性子宫内膜炎时,乳宫安的治愈率为80%,金乳康的治愈率为93%,洁尔阴的治愈率为87%,青链霉素的治愈率为73%。在治疗脓性子宫内膜炎的试验中,乳宫安的治愈率为75%,金乳康的治愈率为88%,洁尔阴的治愈率为75%,青链霉素的治愈率为67%。乳宫安、金乳康和洁尔阴的总受胎率均为100%,而青链霉素的总受胎率为75%。露它净和宫得康配合应用,对卡他性子宫内膜炎的治愈率达100%,对脓性子宫内膜炎的治愈率达90%,并且对愈后奶牛的一次输精受胎率达100%。     

多西环素泡腾片治疗子宫内膜炎和胎衣不下临床效果观察

为探讨多西环素泡腾片对子宫内膜炎和胎衣不下治疗作用,对患病奶牛用多西环素泡腾片进行1～3个疗程的治疗,观察临床效果。结果显示,104例为奶牛子宫内膜炎,治疗后有效率98.77%,治愈率93.27%。126例为奶牛胎衣不下,治疗后有效率98.23%,治愈率95.24%。表明多西环素泡腾片对奶牛子宫内膜炎和胎衣不下有较好的治疗效果。     

盐酸土霉素及清宫液治疗奶牛子宫内膜炎试验

对７７头患子宫内膜炎的奶牛进行治疗试验，其中４６头患牛采用盐酸土霉素治疗，３１头患牛采用清宫液治疗，治疗结果：土霉素治疗组治愈率８０．４３％，好转率１０．８６％，无效８．６％；清宫液治疗组治愈率８７．０９％，好转率６．４５％无效６．４５％。试验结果表明：盐酸土霉素及清宫液治疗奶牛子宫内膜炎的效果显著，可在生产实践中推广应用。     

中草药生物合元制剂对奶牛子宫内膜炎防治试验研究

为解决奶牛子宫内膜炎防治对抗生素及化药的长期依赖，本实验将兽用中草药与益生菌进行功能性技术嫁接，制成中草药生物合元制剂，对奶牛子宫内膜炎进行防治试验研究，结果其临床治愈率为83．33％。与抗生素及化药治疗对照组91．67％治愈率相比虽然存在一定差异，但将药物治疗时间或周期相对延长，其使用效果能满足对奶牛子宫内膜炎的防治需求，可替代或减少抗生素及化药的使用，对当前“无抗奶”的生产具有积极的经济及社会效益。     

抗生素与“清宫促孕宝”治疗奶牛子宫内膜炎疗效报告

[目的]本研究利用抗生素联合中成药治疗子宫内膜炎,为该病有效治疗探索新方法。[方法]使用＂清宫促孕宝＂＋土霉素作为治疗药物,采用子宫内灌注法治疗患牛78例。[结果]试验组78头,有效70头,有效率为89.74%,治愈67头,治愈率为86.0%。[结论]临床治疗表明,＂清宫促孕宝＂＋土霉素治疗各类子宫内膜炎疗效显著,体现了中西医结合的优势。     

Treatment of chronic bovine endometritis and factors for treatment success

In a controlled field trial, 178 dairy cows with chronic endometritis and at least 21 days in lactation were randomly assigned to four different treatment groups: prostaglandin F2alpha intramuscularly (PG, 5 mg dinoprost (5 ml Dinolytic), n = 51), intrauterine antibiotics (AB; 400 mg ampicillin + 800 oxacillin (20 ml Totocillin), n = 49), intrauterine antiseptics (AS; 100 ml 4% Lotagen, n = 50); control (C, no initial treatment, n = 28). Before treatment, uterine swabs for bacteriologic examination and blood samples for determination of serum progesterone concentrations were collected. Two weeks following the first treatment, cows were reexamined. In case no clinical cure was diagnosed, treatment was repeated and control cows were treated for the first time with one of the three treatments mentioned above. The four treatment groups did not differ with respect to the clinical cure or reproductive performance. Therefore, factors that might have an influence on clinical cure and fertility were evaluated. With increasing duration of lactation, the clinical cure after a single treatment increased significantly over all treatment groups from 59.5% (treatment before day 42 postpartum) to 79.6% (treatment following day 42 postpartum) (P < 0.05). Within the PG group, a statistically significantly higher cure rate after a single treatment and first service conception rate and a lower pregnancy index were obtained when the treatment was performed following day 42 postpartum (P < 0.05). This was not the case in the other treatment groups. A retarded involution of the uterus based on the size had a negative effect on clinical cure over all groups (first treatment clinical cure: 68.2% (small uteri) vs 44.4% (large uteri); P < 0.05). Within groups, this effect was also detected, but only as a trend (P > 0.05). Isolation of Arcanobacterium (A.) pyogenes negatively influenced first treatment clinical cure over all treatment groups (79.0% vs 31.5%) and within treatment groups (P < 0.05). In the AB group, the pregnancy index, days open and the interval from first insemination to conception increased compared with the other treatment groups, when A. pyogenes was detected. Isolation of unspecific bacteria and the presence or absence of a corpus luteum only had minor effects over all and within the PG, AS and C group. Within the AB group, presence of luteal tissue was connected with a higher pregnancy index and increased days open and interval from first insemination to conception (P < 0.05). A multivariate analysis revealed that the presence of A. pyogenes and no initial treatment (control group) had a statistically significant independent and negative influence on the first treatment clinical cure. Ill-smelling vaginal discharge and no treatment had a negative independent effect on first service conception rate. Furthermore, days open were negatively influenced by the interval from calving to first examination and the presence of ill-smelling vaginal discharge. In conclusion, under the conditions of this field study, it seems preferrable to treat chronic endometritis. However, under the condition of a herd health program with continuous observation of sick and healthy animals, a wait-and-see attitude might also be justified.     

奶牛子宫内膜炎的诊断及其防治措施

子宫内膜炎是奶牛养殖过程中常见的繁殖类疾病,会推迟奶牛的发情时间,影响配种率.本文首先对急性、慢性奶牛子宫内膜炎的临床症状及其直肠检测结果进行概述,然后从中西医结合、内外兼治的角度提出奶牛子宫内膜炎的治疗方法,该方法能有效缩短疗程,提高治愈率,最后提出加强日常管理、做好产后护理、中药调理精血、掌握好发情规律、适时配种和...     

A Study of Endometritis Causing Repeat Breeding of Cycling Iraqi Buffalo Cows

The objectives of this study were to determine the non‐specific aerobic and anaerobic bacterial causes of endometritis causing repeat breeding of cycling Iraqi buffalo cows at Nineveh province, validate diagnostic criteria for endometritis and to evaluate the treatment efficiency of using systemic or intra‐uterine infusion of antibiotics for the treatment of endometritis. Data were collected from 60 buffalo cows with history of repeat breeding in different herds. All buffaloes were subjected to detailed clinical examination including external inspection, vaginoscopy and transrectal palpation of the cervix, uterus and ovaries. Swabs for bacteriology and biopsies for histopathology were collected from the uterine lumen from each cow. Character, odour and estimation of polymorphonuclear cells (PMN) of the vaginal mucus were scored. Blood samples were collected from cows for creatine kinase (CK) and aspartate aminotransferase (AST) measurement. Treatment conducted using oxytetracycline with tylosin in local intrauterine infusion or systemically with hormonal treatment. The most pre‐disposing factor for uterine infection was retained placenta (13.3%). The most prevalent bacteria in uterine lumen were E. coli (23%), Archanobacterium pyogenes (13%) and Staphylococcus aureus (10%) were mostly isolated from buffaloes with repeat breeding. Vaginal mucus character score was associated with the bacterial growth density score. The difference in PMN was highly significant (p < 0.01) in animals with repeat breeding than control groups. In addition, PMNs was significantly (p < 0.01) correlated r = 0.894 with the character of vaginal discharge. High level of PMNs observed in buffaloes infected with A. pyogenes. Buffalo cows with endometritis had higher CK (321.47 ± 39.06 vs 162.01 ± 16.41 U/l) and AST (133.93 ± 12.43 vs 97.01 ± 6.86 U/l) activities (p < 0.05) than control‐heifers, but no significant difference was observed between buffalo cows with endometritis in CK (321.47 ± 39.06 vs 208.33 ± 5.84) and AST (133.93 ± 12.43 vs 156.17 ± 9.65) activities than control‐pluriparious. It could be concluded that A. pyogenes was the only non‐specific uterine pathogen directly associated with severe endometrial lesions. Vaginoscopy examination combined with palpation of uterus increase the accuracy of diagnosing endometritis and cytogenic examination of uterine discharge is more reliable method of establishing the presence or absence of uterine inflammation in buffalo cows. Animals with repeat breeding (endometritis) showed clinical cure and improved pregnancy in all treatment groups with no significant difference. The use of oestradiol in repeat breeder cases has no effect in improving neither clinical cure rate nor pregnancy rate.     

复方中草药灌注液治疗奶牛子宫内膜炎临床效果观察

目的 研究旨在通过试验观察中草药治疗奶牛子宫内膜炎疗效,以其为临床应用中草药治疗奶牛子宫内膜炎提供参考。方法 将60 头患子宫内膜炎奶牛,随机分成5 组,每组12 头,复方中草药高、中、低剂量组（分别子宫灌注150 mL/头、100 mL/头和50 mL/头）、土霉素组（子宫灌注50 mL/头）和对照组。结果 复方中草药中剂量组和高剂量组的总有效率为75.00%和83.33%,均高于土霉素组的总有效率66.67%;土霉素组在治疗结束后的平均发情天数缩短了14 d,所有复方中草药组的发情天数也缩短了11~19 d;3 个情期内土霉素组受胎率为50.00%,复方中草药组受胎率介于41.67%~66.67%之间。结论 复方中草药对奶牛子宫内膜炎的治疗效果优于土霉素,其中以高剂量组效果最佳。