哈茨木霉菌(Trichoderma harzianum)和终极腐霉菌(Pythium ultimum)对玉米蛋白质组的影响(I)

 哈茨木霉(Trichoderma harzianum) T22菌株已普遍用于防治包括由终极腐霉(Pythium ultimum)引起的苗病或根腐病在内的各种病害。玉米自交系Mo17种子经T22处理后播种在接种腐霉或未接种的田间土壤内,5 d后取幼苗的根系或幼茎提取蛋白。结果表明:在接种腐霉菌的土壤内,未进行T22处理的5 d龄幼苗长势明显比对照差,而经T22处理的幼苗长势明显比对照好。T22和腐霉菌复合处理及T22单独处理对幼苗生长影响基本相同。本研究建立了蛋白质提取和双向电泳分离技术。通过双向电泳及相应的分析软件(PDQuest^TM 2-D softw are)可将不同处理的幼苗自交系蛋白进行分离。T22菌株处理的根系产生104种上游调控蛋白和164种下游调控蛋白,T22与腐霉菌复合处理可产生97种上游调控蛋白和150种下游调控蛋白,而用腐霉菌单一处理诱导的上游或下游蛋白的数量明显少于上述2个处理。T22或腐霉菌单一或复合处理的根系蛋白质组图谱与空白对照相比差异显著,它们与对照的蛋白质组图谱相似系数分别为0.72、0.51和0.49;T22与腐霉菌分别处理的蛋白质组图谱间也相差明显,两者的相似系数仅为0.65。进一步研究发现,T22菌体蛋白质组图谱与上述各种处理的蛋白质组图谱相似系数均很低,说明各种处理诱导后的幼苗根系蛋白质组组分主要来自植物本身,其变化主要因T22或腐霉菌的诱导所致。腐霉菌的侵染对寄主根系蛋白组图谱影响明显高于T22的作用。蛋白质组中各种蛋白质的质谱分析(M ALDI-TOF)与鉴定将另文发表。     

Interactions between the biocontrol agent Pseudomonas fluorescens CHA0 and Thielaviopsis basicola in tobacco roots observed by immunofluorescence microscopy

Pseudomonas fluorescens CHA0 protects plants from damage caused by several soilborne fungi. In this work, immunofluorescence microscopy was used to investigate the colonization of tobacco roots by CHA0 and its physical relationship with the black root rot fungus Thielaviopsis basicola . The pseudomonad colonized the rhizoplane shortly after planting of tobacco seedlings in sterile soil microcosms, in which it had been introduced as soil inoculant. CHA0 was found between and inside cells in the epidermis and the cortex, as well as in the xylem vessels, within 4–7 days after planting of seedlings. The presence of CHA0 delayed the colonization of the interior of tobacco roots by T. basicola compared with the treatment in which only the fungus had been inoculated. Likewise, the pseudomonad reduced the extent of black root rot from 82% to 28%. However, CHA0 was seldom found in contact with the mycelium of T. basicola or in its vicinity, indicating that direct colonization of the mycelium of T. basicola by CHA0 was not required for protection of tobacco against black root rot. Overall, the results suggest that the interior of the root is a key site for implementation of the strain's biocontrol activity against soilborne plant-pathogenic fungi.     

Infection of Norway spruce (Picea abies (L.) Karst.) seedlings with Pythium irregulare Buism. and Pythium ultimum Trow.: histological and biochemical responses

We have studied the reaction of Picea abies seedlings to infection with Pythium. The highly virulent species Pythium ultimum and the less virulent species Pythium irregulare germinated on the root and hypocotyl surface, formed appressoria and penetrated through the stomata as well as through the epidermis. No major differences in the growth of both fungal species were observed during the early events of colonization. The less virulent species formed about 25% more appressoria suggesting that the fungus experienced difficulties with penetration. Differences were observed in the response of the host plant to infection. Autofluorescence, possibly related to deposition of lignin or lignin-like materials increased more in cortical and endodermal tissue colonized with the highly virulent P. ultimum than with the less virulent P. irregulare. Chitinase activity was highest in the tissues most extensively colonized by the fungus. In addition, a systemic increase of chitinase activity was also detected. Interestingly, chitinase activity increased systemically in cotyledons which were never in contact with the pathogen, indicating the translocation of a systemic signal. Salicylic acid was also detected in spruce seedlings; its level increased in roots during infection with the less virulent P. irregulare.     

Sulcotrione soil persistence and mobility in summer maize and winter wheat crops

Sulcotrione soil persistence in spring maize ( Zea mays L.) crops grown on a sandy loam soil was greater at pH 5·5 and 6·0 (soil half-life T _1/2≈58 days) than at pH 7·1 ( T _1/2 = 44 days). Sulcotrione was also applied as recommended on a summer maize crop at the five- to six-leaf growth stage, grown on a sandy loam soil. Sulcotrione soil half-life was 44 days, and the herbicide remained mainly in the 0- to 5-cm surface soil layer during the cropping period, in spite of the high water solubility and the heavy rains at the end of August; lower sulcotrione concentrations (10–18% of the total during the 2-month period after sulcotrione application) were detected in the 5- to 10-cm surface soil layer. The herbicide was applied pre-emergence to winter wheat ( Triticum aestivum L.) at four sites that differed in their soil texture and composition: loamy sand, sandy loam, loam and clay loam. Persistence was greater in the soils containing more organic matter. In soils having similar organic matter contents, persistence was lower in the soil containing more sand relative to loam and clay. During the winter crops, sulcotrione moved down to the 10- to 15-cm soil layer, in spite of the fact that the rains were lower in winter than in summer. Sulcotrione most generally was not detected in the 15–20 cm soil layer of the maize and winter wheat crops.     

Uptake and phytotoxicity of chlorsulfuron in Zea mays L. in the presence of 1,8-naphthalic anhydride

The sites of uptake of chlorsulfuron in maize (Zea mays L.) were investigated at three different growth stages. Exposure of seedling roots, or shoots separately, to herbicide-treated sand over 4 days resulted in inhibition of both roots and shoots. Exposure of seedling roots to chlorsulfuron-treated soil over 21 days severely inhibited both roots and foliage, while separate shoot exposure also reduced both foliage and root growth. After plant emergence, exposure of the crown root node, growing point and lower stem to treated soil reduced foliage and root growth, but exposure of the shoot above the growing point caused only slight inhibition of foliage and had no effect on roots. The herbicide safener 1,8-naphthalic anhydride (NA) applied as a dust (10 g kg^?1 seed weight), or as a 50 mg 1^?1 suspension in water to maize seeds, reduced the root inhibition by chlorsulfuron in 4-day-old seedlings. NA completely prevented both foliage and root injury when chlorsulfuron was placed in soil in the shoot zone before emergence, or in the shoot zone below the soil surface after plant emergence. NA slightly decreased injury to foliage, but not to roots when chlorsulfuron was placed in soil in the root zone before emergence. NA seed treatment protected both roots and foliage against injury from foliarly applied chlorsulfuron. Plants were also protected when a suspension of NA in water was sprayed on the foliage seven days before chlorsulfuron. When a mixture of NA and chlorsulfuron was applied to foliage, root injury was reduced more than foliage injury.     

生物拮抗菌Trichoderma harzianum处理玉米种子诱导实生苗的抗病性分析

 本文研究了生物拮抗菌Trichoderma harzianum(T22)处理对玉米(Mo17)实生苗生长的影响,以及对病原菌Colletrichonum graminicola的抗病性。试验结果表明:用拮抗菌T.harzianum处理后能明显地提高玉米苗根系发育和叶片生长,显著的诱导玉米叶片和根系中防御相关的酶活性和蛋白含量。用病原菌C.graminicola接种玉米叶片后观测发现,处理的实生苗叶片上病斑直径明显小于不处理的对照。尽管拮抗菌T.harzianum诱导植物抗病性的作用已被证实,但该研究更进一步揭示了T.harzianum处理玉米种子后植株的抗性应答与促进实生苗根系发育和叶片生长,以及诱导抗性相关的酶。     

A COMPARISON OF THE GROWTH AND COMPETITION BEHAVIOUR OF SEEDLINGS AND PLANTS FROM RHIZOMES OF AGROPYRON REPENS (L.) BEAUV. AND AGROSTIS GIGANTEA ROTH

Summary. Seedlings and plants derived from single-node rhizome fragments of Agropyron repens and Agrostis gigantea were grown in au unheated glasshouse, separately or together with wheat ( cv. Kolibri), in sandy loam soil in well-spaced pots, adequately watered and moderately fertilized. Samples of crop and weeds were taken for growth analysis in mid- May, late June and early August (when the wheat was ripe).
On average, wheat decreased the weight of weed shoots by 84% and of rhizomes by 77%, but the weeds decreased the weight of wheat shoots by only 7% and of grain by 13%. Without wheat, seedlings of both weed species had, by late June, grown as much as plants from rhizomes, but with wheat, the weed seedlings were throughout most of the experiment more susceptible to competition than were plants from rhizomes. There was no simple relationship between the final dry weights of the weeds and the amount they decreased wheat yield: although Agropyron seedlings in competition with wheat were much lighter than Agrostis from rhizomes, they decreased grain yield of wheat as much, suggesting that they competed more intensely for a limiting factor, possibly nitrogen.
It is concluded that the faster initial growth rate of wheat seedlings relative to the weeds from rhizomes and a larger initial seed reserve relative to the weed seedlings enabled the wheat to dominate the weeds.
Comparaison de la croissance et comportement compétitif de plantules et de plantes issues de rhizomes d'Agropyron repens ( L.) Beauv. et d' Agrostis gigantea Roth     

SHOOT ZONE UPTAKE OF SOIL-APPLIED HERBICIDES*

Summary. Studies were conducted to determine the effects of herbicide placement at different zones of maize (Zea mays L.) and pea (Pisum sativwm L.) shoots below the soil surface after emergence. Soil was removed from around the shoots and replaced with herbicide-treated soil. A wax barrier ensured separate exposure of the zones to treated soil. EPTC, chlorpropham, propham and sulfallate did not affect pea shoot growth, but in maize the shoot zone adjacent to the crown root node was extremely sensitive. Treatment in this area markedly reduced growth and severely inhibited the crown roots. The difference in susceptibility between these species may he due to the location of the growing point relative to the treated soil. Shoots of maize and pea were sensitive to diuron. In maize the shoot adjacent to the crown root node and the tissue of the first internode were the most susceptible. In pea the- uppermost shoot (beneath the soil surface) was the most sensitive. Trifluralin did not affect growth of maize and pea when placed in the shoot zone after emergence, although the crown roots of maize were severely inhibited. Naptalam, dalapon and 2,4-D did not affect growth of maize under similar conditions, and of these only 2,4-D reduced growth of pea. Zone d'abiorption des tiges pour les herbicides appliqués sur h sol     

Studies on the depth distribution and on the germination and growth of Equisetum arvense L (field horsetail) from tubers

Soil core samples taken from a sandy loam soil at Woburn during January 1972, following spring beans during the previous year, indicated that half the rhizome dry weight of Equisetum arvense (L.) occurred in the uppermost 25 cm of soil and 10% between 75 and 100 cm. Tubers occurred relatively more deeply with half the number and three-quarters of the weight deeper than 50 cm. However, after a 2-year fallow 80% of the rhizomes and tubers were in the uppermost 25 cm of soil. In general, the weight of individual tubers increased with increasing depth in the soil. In pots in the glasshouse, tubers that were formed in one season lost weight when new shoots emerged in the following year; those formed during summer germinated readily in warmth indoors when detached in the autumn, but not until June of the following year in the field. Burial of tubers at a range of depths down to 25 cm did not affect the number of shoots emerging but emergence was delayed. Plants made more growth in neutral than in acid or basic soil, more in silty clay loam than in sandy loam and appeared well adapted to growth in soils with little nitrogen. In general, rhizome growth was less affected by variations in pH and nitrogen level than were shoots and tubers. Plants grown from tubers were suppressed greatly by wheat sown densely and given a moderate amount of nitrogen fertilizer but had negligible effect on the wheat. With fewer wheat plants given less nitrogen, the species partitioned more of its assimilate into tubers than when it was grown alone. The significance of tubers in the biology of the species is briefly discussed and areas meriting further study are highlighted.     

Activity,adsorption, mobility and field persistence of sulfosulfuron in soil

Petri dish bioassays, based on root response of corn grown in soil or in perlite, were used to study the activity, adsorption, mobility and field persistence of sulfosulfuron in a silty clay loam and a sandy loam soil. Both bioassays indicated that activity of sulfosulfuron increased with increasing herbicide concentration, and to a slightly greater degree in sandy loam soil than in silty clay loam soil. More sulfosulfuron was adsorbed on the sandy loam (not biologically available) than on the silty clay loam soil. Consequently, slightly greater amounts of sulfosulfuron were leached through the silty clay loam than through the sandy loam soil. Biologically available sulfosulfuron was not detected at depths below 40 cm after application in sandy loam, but this was not the case for the silty clay loam soil. In 2002, all sulfosulfuron rates showed field persistence of less than 5 months. On the other hand, in 2003, biologically available sulfosulfuron was detected in the 0–10-cm soil depth 150 days after application. http://www.phytoparasitica.org posting May 6, 2004.     

浙江省春玉米苗期致病腐霉的研究

作者于１９９０年４、５月间，从浙江省东阳及天台县的玉米病苗上，共分离鉴定到Ｐｙｔｈｉｕｍ ａｃａｎｔｈｏｐｈｏｒｏｍ，Ｐ ａｐｈａｎｉｄｅｒｍａｔｕｍ，Ｐ ｄｅｂａｒｙａｎｕｍ，Ｐ ｇｒａｍｉｎｉｃｏｌａ，Ｐ ｉｒｒｅｇｕｌａｒｅ，Ｐ ｓｐｉｎｏｓｕｍ Ｐ ｔａｒｄｉｃｒｅｓｃｅｎｓ Ｐ ｕｌｔｉｍｕｍ 等８个种的腐霉。接种试验表明，这８种腐霉都能引起玉米苗期根病，造成种子腐烂、根腐和死苗，其中Ｐ     

Effect of method of Imperata cylindrica management on maize grain yield in the derived savanna of south-western Nigeria

Field studies were conducted in 1991 and 1992 on a sandy loam soil in the derived savanna zone of south-western Nigeria to assess the effect of the underground organs of Imperata cylindrica (L.) Raeuschel on maize grain yield. Total dry matter of I. cylindrica in abandoned arable land averaged 16 t ha⁻¹ and rhizomes contributed 56% of this. Rhizome removal from soil before planting maize significantly increased maize yield relative to hoe tillage or slashing of I. cylindrica shoots before planting maize. Digging up rhizomes before planting maize and weeding the crop twice gave a yield reduction of only 23% compared with weed-free crops with rhizomes removed. Grain yield reduction was 51% in plots where rhizomes were only fragmented by hoe tillage and the crop was weeded twice. Grain yield was 62% less when maize was grown in slashed plots with intact rhizomes that were weeded four times (farmers' practice). Slashing without additional weeding resulted in crop failure in maize.     

疏叶骆驼刺幼苗根系生态学特性对水分处理的响应

通过人工壕沟挖掘法,对疏叶骆驼刺幼苗在不同水分条件下的根系生态学特性的季节变化进行研究.结果表明:①在土壤水分较好的环境中,疏叶骆驼刺幼苗大量拓展水平根,并产生分蘖植株竞争光照资源;而在土壤水分相对缺乏时垂直根系发达,向深层土壤拓展资源空间.根系形态的可塑性是疏叶骆驼刺幼苗获取水分适应干旱环境的重要策略之一.②根冠比随土壤水分的减少而增加,且在生长季后期这种趋势更加明显,增加根冠比是疏叶骆驼刺幼苗适应干旱的策略之一.③幼苗根系的扎根深度和垂直生长速度随着土壤水分的减少而增加.④根系生物量、根系表面积随土壤深度的增加而减小,并在垂直剖面上呈倒金字塔状分布.根系生物量形成过程符合Logistic"慢-快-慢"的S型生长曲线,总生物量随着土壤干旱程度的增加而降低.     

GFP标记的球孢白僵菌在玉米中的定殖

为明确球孢白僵菌在玉米中的定殖分布规律以及不同施用方式和处理浓度对玉米生长发育的影响,本文利用绿色荧光蛋白（GFP）标记的球孢白僵菌,在激光扫描共聚焦显微镜下观测了球孢白僵菌在玉米根部、茎部和叶部的定殖情况,同时检测了不同接种方式在球孢白僵菌不同处理浓度下对玉米生长指标的影响。结果表明,球孢白僵菌在玉米不同组织部位上的定殖有着明显的差异,各球孢白僵菌接种处理中,均以叶部定殖的白僵菌孢子数最多,而茎部均未观测到孢子定殖;玉米根部仅在浸种和灌根处理中可见少量孢子。球孢白僵菌不同接种方式对其在玉米中定殖率的影响差异较大,灌根处理定殖率最高,为76.7%;其次为浸种处理,为73.3%;茎部注射处理及叶面喷施处理定殖率较低,分别为43.3%和36.7%。研究表明,不同施用方式及接种浓度球孢白僵菌孢悬液对玉米生长指标有一定的影响,球孢白僵菌可通过不同接种方式在玉米植株中定殖并扩散,对玉米的生长发育有一定的促进作用,其中以1×10⁶~1×10⁷孢子/mL球孢白僵菌孢悬液浸种或灌根,对玉米苗的促进生长作用最明显。     

Deleterious effects of fungi isolated from paddy soils on seminal root of rice

Soil samples were collected from rice paddies at 22 locations in northeastern Honshu, Japan. In 20 of the samples, seedling growth of rice was improved by soil pasteurization (aerated steaming at 60°C for 30 min), although no typical disease symptoms were observed in the seedlings grown in the untreated soil samples. In most locations, rice seedlings grew better in a potting medium containing root material from plants grown in the pasteurized portion of a soil sample than in a medium with root material from plants grown in the unpasteurized portion of the same sample. The results suggest that microorganisms that restrained rice seedling growth may be common in the soils of rice paddies. Approximately 800 isolates were obtained from seedling roots grown in unpasteurized soils and grouped by cultural and microscopic morphologies. The deleterious effects of 79 isolates from 21 major groups, most of which were soil-dwelling taxa, were examined after direct inoculation of the seminal roots. Isolates of Curvularia sp., Cirrenalia sp., Eppicoccum nigrum, Fusarium graminearum, F. oxysporum, Gliocladium virens, Humicola sp., Penicillium sp., Rhizoctonia oryzae-sativae, Sclerotium hydrophilum, Trichoderma aureoviride, and T. harzianum inhibited root growth, suggesting that deleterious root-infecting fungi were more common in paddy soil than previously thought. These fungi may be involved in the restraint of rice seedling growth.     

Prevalence and pathogenicity of spinach root pathogens of the genus Pythium in Sweden

Several pathogenic Pythium spp. were isolated from spinach roots in a 4-year disease survey in commercial spinach fields in southern Sweden. Heterothallic and zoospore-producing isolates belonging to the filamentous, non-inflated zoosporangia (F) group of van der Plaats-Niterink were most abundant, followed by P. sylvaticum, P. ultimum var. ultimum and P. heterothallicum. The heterothallic hyphal swelling (HS) group and P. tracheiphilum were found in a few plants. Two types of pathogenicity tests were performed, to measure the effects of seedling infection and of root infection of older plants. These tests showed P. ultimum var. ultimum to be the most severe spinach pathogen inducing pre- and post-emergence damping off as well as root rot of older plants. Also P. heterothallicum and P. tracheiphilum damaged both seedlings and older plants, Pythium sylvaticum, Pythium 'group HS' and Pythium 'group F'were pathogenic only to seedlings, P. ultimum var. ultimum and Pythium group F were isolated in significantly higher frequencies from spinach grown in the autumn season than in the spring. No clear relationships were found between Pythium prevalence and disease severity index of surveyed field plants, between Pythium prevalence and plant developmental stage, or between prevalence of Pythium and other pathogens isolated. This is the first report of P. heterothallicum and P. sylvaticum being pathogenic on spinach.     

Initial Cellular Interactions Between Thielaviopsis basicola and Tobacco Root Hairs

ABSTRACT Cellular events that occur during the initial interactions between Thielaviopsis basicola and root hairs of tobacco (Nicotiana tabacum) were examined microscopically. Time-course documentation of the infection process indicated a dynamic interaction between T. basicola and the living host cell. Upon root hair contact and recognition, the vegetative apex of T. basicola rapidly differentiated to form infection structures, and the host cell responded cytologically. Penetration was achieved by threadlike hyphae that subsequently developed distal swellings, and intracellular hyphae of sickle-shaped morphology advanced from the distal swelling and colonized the cell. Streaming of the host cytoplasm became aggregated near the infection site prior to penetration and accumulated around the infecting hyphae as long as the host cell was viable. Substantial callose deposition, in the form of a bell-shaped collar around infection structures, resulted from the cytological activity at the infection site. Penetration of dead root hairs was common, but did not lead to the development of infection structures or to a sustained association with the host tissue; T. basicola exited dead root hairs and resumed vegetative growth. The establishment of the parasitic relationship by T. basicola was characteristic of hemibiotrophic fungi in that, initially, biotrophic infection led to tissue colonization, and host cell survival was limited under parasitism.     

An Efficient Inoculation Method for Colonization of Chinese Cabbage Seedlings by the Root Endophytic Fungus Heteroconium chaetospira

To develop an efficient method to inoculate Chinese cabbage seedlings with the root endophytic fungus Heteroconium chaetospira, an appropriate nursery soil and glucose concentration for the nutrient medium for fungal colonization were determined. A grid-sheet method was established for estimating the degree of colonization of entire roots by the fungus. The fungus colonized at high frequencies when peat moss was used as the rooting medium. Colonization was highest (75%) when peat moss was amended with 0.1% glucose. Under these conditions, fungal hyphae developed intracellularly in root cortical cells. In contrast, under high glucose conditions, fungal colonization was restricted mostly to intercellular regions of epidermal or cortical root tissues. Here, hyphae formed inter- or intracellular microsclerotia. Received 19 August 2002/ Accepted in revised form 5 December 2002     

Suppression of Rhizoctonia solani in potato fields. II. Effect of origin and degree of infection with Rhizoctonia solani of seed potatoes on subsequent infestation and on formation of sclerotia

The seed potatoes used in these experiments had been grown in a slightly acid pleistocene sandy soil or in a marine, holocene sandy loam. They were free of sclerotia ofR. solani or lightly or moderately speckled with them. Seed potatoes from the sandy soil produced plants that suffered less fromRhizoctonia than plants from seed potatoes that had been grown on the marine sandy loam. Similarly harvested tubers had, in a non-conducive soil and in conducive soils with a (very) low inoculum density ofR. solani, fewer sclerotia when they came from seed potatoes grown in an acid sand. In each soil, the degree of infestation of the crop not only depended on the severity of infection of the seed potatoes, but also on their origin. With regard to sclerotia production on tubers, three types of soil were distinguished: suppressive, conducive with a high, and conducive with a very low inoculum density ofR. solani. The differences in infestation and in the amounts of sclerotia on tubers between the crop grown from seed potatoes from the sandy soil and that from seed potatoes from the marine sandy loam soil, is attributed to a richer load of antagonists on the former and possibly to a larger proportion of saprophyticRhizoctonia strains among their sclerotia. The antagonists seem to be inhabitants of the subterranean parts of the plant and to function independently of the soil. This implies possibilities for their use in biological control.