R. E. Alexander,M.R.C.V.S.

Extract

The death occurred at Gisborne on September 3, 1964, of Ronald Elward Alexander at the age of 68 years.     

Beef production in the E.E.C. and the co-ordination of research by the Commission of the European Communities

Beef production comprises about 40% of the budget for the 3-year common programme of research in the European Economic Community, granted in 1975 for funding by the Commission. In addition, a full co-ordination programme of seven seminars was held in 1975/1976 on aspects of beef research closely connected with the common programme. The results of about 200 papers and discussion sessions provide a summary of the genetic, nutritional and management aspects of beef production in the Community and some of the research in progress. Many avenues for increased production or improved efficiency of conversion of feed to beef were examined. A survey of breeds and feeds was made; cross-breeding was studied and research on nutrition was reviewed. Methods of increasing the number of calves for beef production were discussed, viz. by egg transfer, by reducing perinatal ill-health, and by the early calving of heifers. An approach to improved efficiency of use of animal and land resources for milk and beef production emerged in terms of the more effective utilisation of the 80 million cattle of the Community and of its crops and by-products. The needs for future research and the common programme of research are briefly described.     

破伤风毒素C片段基因的克隆及在大肠杆菌中的高效表达

本研究对破伤风毒素C片段进行了基因克隆、重组表达、蛋白纯化和免疫原性分析。应用PCR技术直接从破伤风梭菌64008菌株基因组中扩增出大小为1356 bp的破伤风毒素C片段(TetC)基因,经DNA序列测定分析,扩增出的基因与GenBank上登录的序列AF154828的同源性达到99.2%。将此基因克隆至大肠杆菌融合表达载体pGEX-6P-1,构建成重组表达质粒pGEX-6P-1-TetC,并在大肠杆菌BL21中表达,重组蛋白的表达量占菌体总蛋白的21%。经SDS-PAGE蛋白电泳鉴定,表达产物为76 Ku左右的重组蛋白,经免疫印迹试验证实该重组蛋白是破伤风毒素C片段抗原。