枯草芽孢杆菌和酵母培养物联合添加在断奶仔兔上的应用效果研究

试验旨在研究饲粮中添加枯草芽孢杆菌和酵母培养物,对伊拉肉兔生产性能、养分消化率、血清免疫、抗氧化和抗应激能力的影响。选择396只35日龄、体重为（0.979±0.037） kg健康的断奶伊拉肉兔,按随机区组设计分为4个处理（每个处理11个重复,每个重复9只兔）,分别为基础饲粮（对照组）、添加1 000 mg/kg枯草芽孢杆菌、添加3 000 mg/kg酵母培养物、添加1 000 mg/kg枯草芽孢杆菌+3 000 mg/kg酵母培养物（联合添加组）。试验期共30 d。结果表明：与对照组相比,饲粮添加3 000 mg/kg酵母培养物,可提高断奶仔兔1～15 d平均日增重（ADG）,降低料重比（F/G）(P<0.05);联合添加显著提高断奶仔兔16～30 d平均日增重,降低料重比（P<0.05）;与对照组相比,3个处理组均可提高断奶仔兔粗蛋白（CP）的表观消化率（P<0.05）;饲粮添加3 000 mg/kg酵母培养物或与1 000 mg/kg枯草芽孢杆菌联用可提高断奶仔兔干物质（DM）和粗脂肪（EE）的表观消化率（P<0.05）;联合添加可提高断奶仔兔粗纤维（CF...     

日粮添加不同水平酵母硒对肉羊生长性能、肉品质及血液生化指标的影响

为研究酵母硒（SY）对肉羊生长性能、肉品质及血液生化指标的影响,试验选择80只体况良好、体重（26.18±2.00）kg的肉羊随机分成4组,每组4个重复,每个重复5只羊。对照组饲喂基础日粮,酵母硒组在基础日粮中分别添加0.2、0.3、0.4 mg/kg的SY。预饲期7 d,试验正式期10周。结果表明：日粮中添加0.3 mg/kg酵母硒组肉羊的平均日增重显著高于对照组（P < 0.05）,提高了17.88%。日粮中添加0.3 mg/kg酵母硒组肉羊的料重比显著低于对照组和0.4 mg/kg酵母硒组（P < 0.05）,分别降低了11.31%和16.05%。日粮中添加0.3 mg/kg和0.4 mg/kg酵母硒组肉羊的肉色红度a*显著高于对照组（P < 0.05）,分别提高了10.51%和13.79%|日粮中添加0.3 mg/kg和0.4 mg/kg酵母硒组的pH45 min显著高于对照组（P < 0.05）,分别提高了6.11%和4.98%|日粮中添加0.3 mg/kg酵母硒组的剪切力显著低于对照组和0.4 mg/kg酵母硒组（P < 0.05）,分别降低了10.92%和13.42%|日粮中添加0.3 mg/kg酵母硒组的熟肉率显著高于对照组和0.4 mg/kg酵母硒组（P < 0.05）,分别提高了4.82%和7.43%。日粮中添加0.3 mg/kg和0.4 mg/kg酵母硒组的总蛋白含量显著高于对照组（P < 0.05）,分别提高了8.49%和12.59%|日粮中添加0.4 mg/kg酵母硒组的尿素氮和肌酐含量显著高于对照组、0.2 mg/kg酵母硒组、0.3 mg/kg酵母硒组（P < 0.05）,分别提高了11.67%、12.84%、14.78%|18.90%、15.10%、11.67%。综上所述,在肉羊日粮中添加酵母硒可以提高其生长性能,改善肉品质,并提高血液中总蛋白含量,本试验条件下,最适添加量为0.3 mg/kg。 [关键词] 酵母硒|生长性能|肉品质|血液生化指标|肉羊     

饲粮中添加酵母硒对湖羊生长性能的影响

本试验旨在研究酵母硒对湖羊生长性能的影响。选取体重相近、日龄相近、健康的湖羊150只，平均分为5组，对照组饲喂基础日粮，试验组分别在基础日粮中添加0.3mg/kg、0.6mg/kg、0.9mg/kg、1.2mg/kg酵母硒。预试期10d，正试期60d。结果表明：饲料中添加酵母硒对湖羊平均日采食量没有影响；添加0.3～1.2 mg/kg酵母硒可显著增加湖羊平均日增重（P<0.05），添加0.6～1.2mg/kg酵母硒可显著降低湖羊料重比（P<0.05），说明饲料中添加酵母硒可以提高湖羊的生长性能。综合酵母硒对湖羊生长性能的影响，建议在湖羊饲料中添加0.6～0.9mg/kg酵母硒。     

不同硒源对育肥初期徐淮白山羊抗氧化能力、表观消化率及生长性能影响的研究撤回

旨在研究饲粮中添加不同硒源对育肥初期徐淮白山羊抗氧化能力、营养物质表观消化率及生长性能的影响。选取断奶健康徐淮白山羊50只,随机分为5组,每组2个重复,每个重复5只,对照组(C组)饲喂基础饲粮,试验组饲粮中分别添加以硒含量计算的0.3 mg/kg亚硒酸钠(0.3 SS组)、0.3 mg/kg酵母硒(0.3 SY组)、0.3 mg/kg硒代蛋氨酸(0.3 SM组)和0.5 mg/kg硒代蛋氨酸(0.5 SM组),预饲期15 d,试验期60 d。结果显示：1)血液抗氧化指标GSH-Px、T-AOC和SOD活性在0.3 SY和0.3 SM组均显著高于C组或0.3 SS组(P<0.05);0.3 SM组MDA含量最低,且显著低于C组或0.3 SS组(P<0.05)。2)各试验组CP、EE、NDF和ADF表观消化率均显著高于C组(P<0.05);0.3 SM组CP、NDF、ADF的表观消化率显著高于0.3 SY组(P<0.05);与0.3 SM组相比,0.5 SM组随着硒代蛋氨酸浓度的增加营养物质表观消化率有所降低。3)平均日增重0.3 SY组显著高于C组或0.3 SS组...     

不同水平酵母硒对奶牛血液抗氧化能力的影响

本研究旨在通过在奶牛日粮中添加酵母硒,探讨其对奶牛血液抗氧化能力和泌乳性能的影响,为酵母硒在奶牛养殖业中的应用提供参考依据。试验选取40头体况、奶产量、泌乳天数相近的奶牛,分为4组,对照组添加亚硒酸钠,添加后硒水平为0.3 mg/kg日粮,3个处理组在日粮基础上添加酵母硒,添加后日粮硒水平分别为:0.3、0.4 5和0.6 mg/kg。对试验牛群乳样和血样分析表明:与亚硒酸钠相比,添加酵母硒可以极显著降低乳中体细胞数(P<0.0 1),-提高全血硒含量及血清中总抗氧化能力(T-AOC),超氧化物歧化酶(SOD)和谷胱苷肽过氧化物酶(GSH-PX)的活力(P<0.0 1);在0.3 mg/kg硒水平下,与亚硒酸钠相比饲喂酵母硒奶牛血清丙二醛(MDA)含量显著降低(P<0.0 5),综合考虑,日粮硒水平为0.4 5 mg/kg的酵母硒添加组效果较好。     

酵母硒添加水平对肉鸭胴体品质、组织硒含量及抗氧化性能的影响

文章旨在研究日粮添加不同水平有机硒(酵母硒形式)对1~48 d肉鸭生长性能、胴体品质、组织硒含量及抗氧化性能的影响。试验选择平均体重为(56.66±0.14)g的商品肉鸭720只,随机分为4组,每组4个重复,每个重复45只。对照组不添加酵母硒,其他3组分别在基础日粮中添加0.15、0.30和0.45 mg/kg硒(以酵母硒形式),试验共进行48 d。结果显示:0.3 mg/kg酵母硒组较对照组和0.45 mg/kg酵母硒组显著提高了48 d肉鸭的体重(P0.05);0.45 mg/kg酵母硒组较0.15和0.30 mg/kg酵母硒组显著降低了肉鸭冷胴体重(P0.05);对照组冷胴体占比显著高于0.45 mg/kg酵母硒组(P0.05)。与对照组相比,0.3和0.45 mg/kg有机硒组显著降低了肌肉的pH45min(P0.05);对照组和0.15 mg/kg有机硒组屠宰后45 min肌肉温度显著高于0.3和0.45 mg/kg有机硒组(P0.05);对照组胸肌和腿肌水分含量显著高于其他各组(P0.05);0.45 mg/kg酵母硒组胸肌蛋白质含量显著高于对照组和0.15 mg/kg有机硒组(P0.05),而对照组和0.45 mg/kg有机硒组腿肌脂肪含量显著高于对照组(P0.05)。随着日粮有机硒添加水平的升高,血清GSH-Px活力和硒水平表现为显著升高(P0.05)。随着日粮有机硒添加水平的升高,血清、肝脏、粪和肌肉中硒的水平显著升高(P0.05)。高水平有机硒(0.45 mg/kg)显著降低了14 d肉鸭硒的吸收效率(P0.05),但显著提高了48 d肉鸭硒的吸收效率(P0.05)。结论 :有机硒可以显著提高肉鸭血清、肝脏和肌肉中的硒含量以及血清GSH-Px活力,其中日粮添加0.3 mg/kg硒(酵母硒)可以显著提高1~48 d肉鸭的体重。     

硒酵母对西门塔尔牛日粮养分消化代谢和血清生化指标的影响

本试验旨在研究硒酵母对西门塔尔牛营养物质消化代谢和血清生化指标的影响。选用16头平均体重420 kg,年龄2.5岁的西门塔尔牛阉牛,采用随机区组设计分为4组,以硒酵母为硒源,分别在日粮中添加硒0、0.3、0.6和0.9 mg/kg。结果表明:日粮养分表观消化率、矿物元素表观存留率、消化氮和沉积氮/消化氮比例均以0.3 mg/kg与0.6 mg/kg组较高(P<0.05);0.6 mg/kg组甘油三酯显著提高,处理组血糖显著增加,0.6 mg/kg和0.9 mg/kg组白蛋白增加(P<0.05),总胆固醇、总蛋白和尿素氮差异不显著(P>0.05);0.6 mg/kg和0.9 mg/kg组血清GOT和GPT含量显著提高(P<0.05),AKP、ACP和LDH差异均不显著(P>0.05);处理组GSH-px活性显著提高(P<0.05),以0.6 mg/kg组最高;0.6 mg/kg和0.9 mg/kg组MDA显著降低(P<0.05);0.6 mg/kg组GSH含量显著增加(P<0.05);0.6 mg/kg和0.9 mg/kg组血清硒含量显著增加(P<0.05)。日粮以硒酵母为硒源时,添加硒0.6 mg/kg显著促进营养物质消化代谢和提高机体抗氧化能力,建议日粮加硒为0.6 mg/kg。[动物营养学报,2007,19(4):379-385]     

富硒恩施堇叶碎米荠对断奶仔猪生长性能、血清抗氧化及免疫功能的影响

为研究富硒恩施堇叶碎米荠对断奶仔猪生长性能、血清抗氧化和免疫功能的影响，试验选用864头健康、初始体重（8.03±1.24）kg断奶仔猪，按照体重和性别进行完全随机区组分为4个处理，每个处理12个重复，每个重复18头猪。日粮处理组分别为：（1）对照组，无硒源添加剂的基础日粮；（2）亚硒酸钠组，0.3 mg/kg硒来源于亚硒酸钠+基础日粮；（3）酵母硒组，0.3 mg/kg硒来源于酵母硒+基础日粮；（4）碎米荠硒组，0.3 mg/kg硒来源于碎米荠+基础日粮。试验共28 d,1～14 d为试验前期，15～28 d为试验后期。结果表明：与亚硒酸钠和酵母硒相比，在试验前期和后期，日粮中添加富硒恩施堇叶碎米荠均可降低断奶仔猪的料重比和腹泻率（P <0.05），综合全期来看，碎米荠硒组仔猪的料重比显著低于对照组（7.56%）和酵母硒组（5.92%）仔猪（P <0.05）。在试验前期，碎米荠组仔猪血清谷胱甘肽过氧化物酶（GSH-Px）活性显著高于亚硒酸钠组（7.45%）和酵母硒组（10.18%）仔猪（P <0.05），其血清丙二醛（MDA）水平也显著低于亚硒酸钠组（29.52%）...     

富硒酵母对分娩后奶牛采食量、血液生化及激素水平的影响

文章旨在探讨日粮添加富硒酵母对分娩后奶牛采食量、血液生化及激素水平的影响。试验将分娩后28?d、平均初始体重为（616.97±4.51）kg的20头奶牛随机分为2组，每组5个重复，每个重复2头，对照组饲喂基础全混合日粮（以亚硒酸钠的形式补充0.2?mg/kg硒），处理组饲喂基础全混合日粮+2?g/kg富硒酵母（硒含量为100?mg/kg）。试验从分娩后28?d持续至112?d。结果：对照组与富硒酵母组对奶牛分娩后28、56、84和112?d的采食量和体重的影响均无显著差异（P＞0.05）。对照组与富硒酵母组对奶牛分娩后不同时期血清葡萄糖和尿素氮浓度的影响也无显著差异（P＞0.05）。与对照组相比，富硒酵母组奶牛分娩后28、56、84和112?d血浆硒浓度分别显著提高57.60%、62.12%、62.01%和61.88%（P＜0.05）。与对照组相比，富硒酵母组奶牛分娩后56和84?d血浆孕酮浓度分别显著提高66.15%和80.10%（P＜0.05）。结论：全混合日粮添加2?g/kg富硒酵母可以显著提高奶牛分娩后28～112?d血浆硒浓度及分娩后56和84?d血浆孕酮浓度。 [关键词]富硒酵母|奶牛|采食量|血液生化|激素水平     

酵母硒添加水平对育肥猪硒元素消化率和沉积量影响

为探讨育肥猪日粮中酵母硒的添加水平对硒元素消化率的影响,试验选用育肥猪60头,随机分为3组,试验组日粮中硒元素分别为0.3mg/kg、0.6mg/kg、0.9mg/kg。酵母硒中的硒含量为1935mg/kg。结果表明,试验组育肥猪的表观消化率分别为89%、87%、87%,各组间结果差异不显著;硒的沉积量为1.2784mg、1.9034mg、2.5305mg,各组间结果差异显著,沉积量和硒添加水平的线性关系为y=0.648x+0.0135。不同酵母硒的添加水平对育肥猪硒元素的消化率没有影响,硒元素沉积量与酵母硒的添加水平有线性关系。     

Milk selenium content and speciation in response to supranutritional selenium yeast supplementation in cows

The effects of selenium (Se) yeast supplementation on performance, blood biochemical and antioxidant parameters, and milk Se content and speciation were evaluated. Thirty-six mid-lactation Holstein dairy cows were randomly assigned to 1 of 3 treatments: 1) control (basal diet containing Se at 0.11 mg/kg DM), 2) basal diet + 0.5 mg supplemental Se/kg DM (SY-0.5), and 3) basal diet + 5 mg supplemental Se/kg DM (SY-5). Selenium was supplemented as Se yeast. The trial consisted of a 1-week pretrial period and an 8-week experimental period. Milk somatic cell score decreased with SY-5 supplementation (P < 0.05), but other performance parameters were not affected (P > 0.05). The serum Se concentration increased with the increasing levels of Se yeast supplementation (P < 0.05), however, blood biochemical parameters showed few treatment effects. The antioxidant capacity of dairy cows was improved with Se yeast supplementation reflected in increased serum glutathione peroxidase activity (P < 0.05) and total antioxidant capacity (P = 0.08), and decreased malondialdehyde concentration (P < 0.05). Milk total Se concentration increased with Se dose (P < 0.05). Also, the selenomethionine concentration increased with Se dose from 13.0 ± 0.7 μg/kg in control to 33.1 ± 2.1 μg/kg in SY-0.5 and 530.4 ± 17.5 μg/kg in SY-5 cows (P < 0.05). Similarly, selenocystine concentration increased from 15.6 ± 0.9 μg/kg in control and 18.9 ± 1.1 μg/kg in SY-0.5 to 22.2 ± 1.5 μg/kg in SY-5 cows (P < 0.05). In conclusion, Se yeast is a good organic Se source to produce Se-enriched cow milk with increased Se species including selenomethionine and selenocystine. The results can provide useful information on milk Se species when a high dose Se yeast was supplemented in the cow diet.     

添加不同水平莫能霉素对奶牛瘤胃内日粮营养物质发酵及血液生化指标的影响

[目的]该试验旨在探讨日粮中添加不同水平莫能霉素,对奶牛瘤胃内发酵、日粮营养物质降解率及奶牛血液生化指标影响。[方法]试验选取3头安装永久性瘤胃瘘管奶牛,设计采用3×3拉丁方试验设计。试验分为三组,MⅠ组为基础日粮;MⅡ组为基础日粮添加31.5mg/kg DM莫能霉素;MⅢ组基础日粮添加46.5 mg/kg DM莫能霉素。[结果]莫能霉素添加组pH值均高于对照组（P〉0.05）;添加46.5 mg/kg DM的莫能霉素后氨态氮浓度显著低于对照组（P〈0.05）;日粮中添加46.5 mg/kg DM的莫能霉素后瘤胃内乙酸发酵量显著低于对照组（P〈0.05）;日粮中添加31.5 mg/kg DM莫能霉素后丙酸发酵量高于对照组（P〉0.05）,且丙酸摩尔比例显著高于对照组（P〈0.05）;日粮DM、CP和NDF在瘤胃内有效降解率随莫能霉素添加量的增加而降低,添加组奶牛血液中的尿素氮,血浆中血糖及乳酸浓度,各组间差异不显著（P〉0.05）。[结论]日粮中添加莫能霉素可以提高牛瘤胃中的pH值,降低氨态氮浓度,显著提高丙酸摩尔比例,降低丙酸、乙酸比值,但减缓了粗蛋白的降解速度,一定程度上提高血液中尿素氮和血糖浓度。     

Effect of selenate as a feed supplement to dairy cows in comparison to selenite and selenium yeast

The main aim of this trial was to define the possible differences between selenite and selenate in their ability to increase the selenium (Se) concentration of milk, in comparison with organic Se. Dairy cows (n = 42) were fed a basal diet containing .10 to .12 mg Se/kg DM for 5 mo and were then divided into four groups of 10 or 11, as similar as possible in age and stage of lactation. During the next 84 d, the cows in three of the groups were supplemented with 3 mg of Se daily, whereas the cows in one control group remained unsupplemented. The Se supplement was given as sodium selenite, sodium selenate, or a Se yeast product. The total Se concentration of the diets varied with the cows' stage of lactation and was for the supplemented groups .24 to .31 mg/kg DM, but remained between .10 and .12 mg/kg in the control group. At the end of the trial, the mean whole blood Se concentrations in the selenite, selenate, yeast, and control groups were 138, 141, 165, and 104 microg/L, respectively. The Se concentration in plasma apparently reached a plateau level within 4 wk, at approximately 75 microg/L in the selenite group, 80 microg/L in the selenate group, and 90 microg/L in the yeast group. In the control group the mean concentration in plasma remained at approximately 50 microg/L. The increase of the activity of glutathione peroxidase (GSH-Px) in the erythrocytes was significantly higher in the supplemented groups than in the control group. The mean concentrations of Se in milk in the selenite, selenate, and yeast groups were 16.4, 16.4, and 31.2 microg/L, respectively, whereas the concentration remained at approximately 14 microg/L in the control group. The milk Se concentration reached a plateau within 1 wk after the start of Se supplementation. Dietary supplementation with selenite and selenate, thus, had only a limited effect on the Se concentration in milk, and there was no significant difference between the two inorganic compounds in any variable measured. Organic Se was much more effective than inorganic Se in increasing the concentration of Se in milk.     

Increasing selenium supply during the close-up dry period improves nutrient metabolism and attenuates inflammatory response after calving in dairy cows

The objective of this study was to investigate whether feeding selenium (Se)-replete cows a Se-yeast supplement in late pregnancy affects nutrient metabolism and inflammatory response during the periparturient period. Twenty cows were randomly assigned to two groups with 10 cows each. Cows in one group received Se-yeast at 0.3 mg Se/kg DM during the last 4 weeks before calving in addition to fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Se-yeast), while cows in another group were only fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Control). Blood samples were collected and analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHBA), glucose, insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, serum amyloid A (SAA), haptoglobin (Hp), and albumin. In control cows, plasma NEFA, IL-1β, IL-6, SAA, and Hp levels increased after calving, but glucose, insulin, and albumin levels decreased after parturition. Se-yeast supplemental cows had lower postpartum concentrations of NEFA, TNF-α, IL-1β, IL-6, SAA, and Hp, and higher postpartum levels of glucose, insulin, and albumin compared with control cows. The results indicate that feeding Se-replete cows a Se-yeast supplement in late pregnancy improves nutrient metabolism and attenuates the inflammatory response after calving.     

奶牛日粮添加豆油和胡麻油对有机物和纤维物质消化率的影响

选用4头装有永久性瘤胃瘘管的初产奶牛采用4×4拉丁方设计，研究不添加油脂（CK）、添加4％豆油（LOC1）、4％胡麻油（LOC2）以及2％豆油+2％胡麻油（LOC3）对DM、OM、NDF、ADF瘤胃表观消化率及全肠道消化率的影响。结果表明：在60%高粗料日粮基础上添加4%豆油、亚麻油或二者的混合物，对奶牛干物质采食量没有影响。与对照组相比,添加油组DM、OM、NDF、ADF的瘤胃表观消化率和全肠道消化率都有降低的趋势，但差异不显著（P＞0.05），3个添加油组之间差异也不显著（P＞0.05）。     

不同硒源对泌乳中期荷斯坦奶牛生产性能、抗氧化性能及营养物质表观消化率的影响

本试验旨在研究不同硒源对泌乳中期荷斯坦奶牛生产性能、抗氧化性能及营养物质表观消化率的影响。试验选取45头产奶量、胎次、泌乳日龄相近的健康中国荷斯坦奶牛,随机分成3组,每组15头。对照组饲喂不添加硒的基础饲粮,无机硒(SS)组饲喂基础饲粮+0.3 mg/kg亚硒酸钠,有机硒(SO)组饲喂基础饲粮+0.3 mg/kg蛋氨酸硒羟基类似物。试验期91 d,其中预试期7 d,正试期84 d。结果表明:1)饲粮中添加硒对奶牛的干物质采食量、产奶量及乳成分没有显著影响(P0.05)。2)与对照组相比,SO组血清总抗氧化能力和谷胱甘肽过氧化物酶活性显著升高(P0.05),血清丙二醛含量显著降低(P0.05),但SS组以上指标与对照组没有显著差异(P0.05)。3)各组中性洗涤纤维、酸性洗涤纤维和干物质的表观消化率差异不显著(P0.05)。与对照组相比,SO组有机物、粗蛋白质及粗脂肪的表观消化率显著提高(P0.05);SS组有机物和粗蛋白质的表观消化率提高,但差异不显著(P0.05)。综上所述,饲粮添加不同硒源对泌乳中期荷斯坦奶牛的生产性能没有影响,但与对照组相比,硒添加组的抗氧化性能和营养物质的表观消化率提高,而且SO组的效果明显优于SS组。     

不同水平的蛋氨酸铜对奶牛瘤胃内环境及日粮表观消化率的影响

[目的]为探讨日粮中添加不同水平的蛋氨酸铜对奶牛瘤胃内环境及日粮表观消化率的影响。[方法]在日粮中分别添加0 mg/kg、18 mg/kg、27 mg/kg DM的蛋氨酸铜,采用3×3拉丁方试验设计,试验动物为3头装有永久性瘤胃瘘管的中国荷斯坦奶牛,平均体重500 kg,均处于干奶期,试验基础日粮由混合精料、羊草和玉米全株青贮组成,精粗比为30∶70。[结果]表明,日粮添加蛋氨酸铜有提高瘤胃pH值、NH3-N的趋势;但对各时间点的瘤胃液pH值、NH3-N没有显著影响（P〉0.05）;添加27 mg/kg DM的蛋氨酸铜显著提高了日粮各个营养物质的表观消化率。[结论]添加蛋氨酸铜不影响奶牛瘤胃的内环境,添加27 mg/kgDM的蛋氨酸铜可以提高日粮的表观消化率。     

叶黄素和维生素C对高温环境中蛋鸡生产性能及蛋品质的影响

研究了万寿菊叶黄素(Marigold Lutein,MAR)和维生素C(VC)对高温环境下罗曼粉壳蛋鸡生产性能和蛋品质的影响规律。将216只180日龄身体健康、体重相似的罗曼粉蛋鸡作为试验动物,随机分为6个组,每组设3个重复,每个重复12只鸡。试验1组为高温对照组,饲喂基础日粮,试验2组饲喂基础日粮+200mg/kg VC,试验3、4、5、6组在试验2组的基础上,分别添加60mg/kg、120mg/kg、180mg/kg、240mg/kg万寿菊叶黄素(分别组成复合剂Ⅰ、Ⅱ、Ⅲ、Ⅳ)。试验期56d,其中预试期14d,正式期42d。研究结果表明,在长期高温环境中,日粮中添加120mg/kg MAR+200mg/kg VC,可有效提高蛋鸡生产性能,其中采食量、产蛋率、平均蛋重分别较对照组提高26.79%(P<0.01)、13.87%(P<0.05)和4.1%(P<0.01),破壳率降低53.49%(P<0.01),死淘率为0;同时可有效改善蛋品质,与对照组比较,蛋壳厚度、哈氏单位、蛋黄相对重有提高的趋势(P>0.05);蛋黄RCF值提高2.12(P<0.01)、蛋黄红度(a*)提高90.37%(P<0.01)、蛋黄黄度(b*)提高11.36%(P<0.01)。