小麦黄矮病(BaYDV)红穗症状研究

 小麦黄矮病(BaYDV)主要症状特征是叶片褪绿黄化或红化和植株矮化。发生红穗症状未见报道。     

3种菊科入侵植物白花鬼针草、胜红蓟和假臭草的种子生物学特性比较研究

为探讨菊科入侵植物种子的繁殖特性,从种子的形态特征及种子萌发率等指标入手,对胜红蓟(Ageratum conyzoide)、白花鬼针草(Bidens alba)及假臭草(Eupatorium catarium)3种菊科入侵植物的种子特性进行比较研究。结果表明,白花鬼针草种子的冠毛芒最短及最少,沉降速度最大,种子的风媒传播能力最弱,但种子全身具长倒钩刺,主要通过人畜和货物等实现远距离散播。假臭草种子冠毛芒较胜红蓟的长和多,种子沉降速度较胜红蓟的小,其风媒传播能力较胜红蓟的强。白花鬼针草种子千粒质量、发芽指数、胚根、胚芽和胚轴均最大或最长,发芽率和发芽势也较高,其种子质量和种子活力为三者中最高。假臭草的千粒质量、胚根、胚芽和胚轴与胜红蓟无显著差异,但发芽势及发芽指数均显著高于胜红蓟,说明假臭草种子活力较胜红蓟的高。假臭草发芽率为三者中最小,这可能与其种子含水率(19%)高有关,高含水率隐含着种子在潮湿环境下易发生霉变。胜红蓟种子除了发芽率较高外,千粒质量、发芽势、发芽指数、胚根、胚芽和胚轴等指标均较小或大,反映其种子质量和活力较小。     

韩国首次报道蓝莓红环斑病毒侵染蓝莓

蓝莓红环斑病毒(Blueberry red ringspot virus,BRRSV)属Caulimovididae科大豆萎黄(退绿)斑驳样病毒属(Soymovirus),引起蓝莓(Vaccinium corymbosum)叶、茎和果的红环斑病。美国、日本、捷克、斯洛文尼亚、     

三种红瓢虫的识别及雄性外生殖器的描述

草履蚧Drosicha corpulenta(Kuwana)是北方园、林、果树上的重要害虫,而红瓢虫属Rodolia Mulsant的昆虫是其重要天敌。本文对北京园林植物上的三种红瓢虫(暗红瓢虫Rodolia concolor(Lewis)、红环瓢虫Rodolia limbata(Motschulsky)和浅缘瓢虫Rodolia ru- focincta(Lewis)进行研究,描绘了它们的外生殖器及鉴别特征,其中对浅缘瓢虫外生殖器的描述尚属首次。     

利用茎尖嫁接脱毒培养无裂皮病的脐血橙

脐血橙是六十年代从摩洛哥引入的优良品种。在试种中表现果实品质好和晚热等性状,有推广前途。但是这个品种的植株普遍受裂皮病感染。在用枳或(木黎)檬(如广西的土柠檬)作砧木时,砧木树皮严重开裂,植株显著矮化、衰退,产量很低,甚至整株死亡。在用耐病的红桔或酸桔作砧木时,植株     

不同电子载体对丙酮丁醇发酵的影响研究

通过研究不同浓度的丁酸钠、乙酸钠、中性红及组合添加中性红与弱酸盐等电子载体对菌种ART18发酵木薯粉产丁醇的影响。结果表明,丁酸钠、乙酸钠以及中性红均可提高丁醇产量,其中中性红效果最为显著,当添加量为3.0 g/L时,丁醇产量可达到(11.3±0.23)g/L,较对照组相比提高了51.7%;当组合添加3.0 g/L中性红与3.0g/L丁酸钠时,丁醇和总溶剂产量进一步提升,分别达到了(12.2±0.25)g/L和(17.4±0.18)g/L,较对照组分别提高了64.9%和33.8%,与单独添加丁酸钠和中性红相比,丁醇产量分别提高了32.6%和8.0%。说明组合添加中性红与弱酸盐对丁醇发酵有一定促进作用。     

红橘褐斑病的名称、发生与防治对策

2007年以来红橘褐斑病Alternaria alternata(Fr.)Keissler在重庆万州暴发、蔓延,为害程度逐年加重,发病面积不断扩大。该病在当地主要为害红橘(品种大红袍),椪柑、伊予柑(橘和柚的杂交种)也可发病,但与严重发病的红橘同在一个橘园的甜橙并不受害。该病在红橘春梢期、幼果期和果实膨     

不同生态条件下葡萄叶部微生物区系分析

采用稀释平板法,对兰州市红古区露地和天祝县日光温室栽培‘红地球’葡萄叶部微生物进行分离计数和丝状真菌的纯化鉴定。结果表明,红古露地栽培葡萄叶部微生物的数量显著高于天祝日光温室。从幼果膨大期到果实成熟期,真菌数量均逐渐增加到最大值,其中红古葡萄叶部为2.89×102cfu/cm2,天祝葡萄叶部为0.49×102cfu/cm2,采收后均略有下降。细菌数量,红古葡萄叶部的变化与真菌相似,最高达12.68×102cfu/cm2,天祝葡萄叶部则在果实着色期达到最高值1.27×102cfu/cm2,后下降并趋于稳定。红古葡萄叶部的真菌种类和出现频率有一定的连续性,其中链格孢属(Alternaria)、盾壳霉属(Coniothyrium)、芽枝霉属(Cladosporium)在红古和天祝两种生态条件下均占优势,出现频率最高分别达到56.19%、44.3%和45.45%。青霉属(Penicillium)只在红古葡萄叶部分离到,根霉属(Rhizopus)和曲霉属(Aspergillus)只在天祝葡萄叶部分离到。筛选出21种对葡萄灰霉病菌菌丝有抑制效果的拮抗菌。     

红颈常室茧蜂对绿盲蝽及其为害寄主植物的选择行为

为明确红颈常室茧蜂Peristenus spretus Chen et van Achterberg定位寄主绿盲蝽Apolygus lucorum(Meyer-Dür)时利用的挥发性信息物质来源,利用Y型昆虫嗅觉仪测定了红颈常室茧蜂雌雄成虫对绿盲蝽3龄若虫及其为害寄主植物棉株以及雌性红颈常室茧蜂成虫、绿盲蝽成虫对苗期和花期的棉花、蓖麻的选择行为。结果表明,与干净空气(空白对照)相比,雌性红颈常室茧蜂对绿盲蝽3龄若虫、健康棉株的选择行为无显著差异,绿盲蝽3龄若虫为害的棉株对雌性红颈常室茧蜂具有显著的吸引作用,但在为害后去虫和未去虫处理间无显著差异;雄性红颈常室茧蜂对各处理均无明显的选择行为。与健康或为害后去虫的苗期棉花相比,雌性绿盲蝽成虫显著趋向花期蓖麻;而雌性红颈常室茧蜂只趋向于为害后的花期蓖麻。雌性红颈常室茧蜂对为害后去虫的花期棉花和花期蓖麻的选择行为显著高于相应的苗期植株。这表明雌性红颈常室茧蜂主要利用绿盲蝽若虫为害后寄主植物释放的挥发性信息物质来定位寄主绿盲蝽,且其对花期寄主植物的选择行为与绿盲蝽成虫具有一致性。     

烯效唑对苗期低温胁迫下红小豆产量及茎部抗逆生理指标的影响

以耐冷性不同的2个红小豆(Vigna angularis)品种宝清红(耐冷型)和天津红(冷敏型)为材料,盆栽条件下于苗期在人工气候室进行低温(15℃,分别持续1、2、3、4、5 d)及喷施烯效唑(S_(3307))处理,研究苗期低温胁迫下烯效唑对红小豆产量和茎部逆境生理指标的影响。结果表明:幼苗期低温引起红小豆茎部SOD活性提高,宝清红在处理第2天达到最高,较CK(室外常温)高15.35%;而天津红在处理第3天时达到最高,较CK高20.73%。POD和CAT活性也有所提高,处理3 d时酶活性最高,与CK相比较,宝清红和天津红分别提高了75.31%和4.55%、63.50%和22.22%。低温处理也导致红小豆植株茎部MDA含量增加,低温处理5d后,天津红和宝清红茎部MDA含量分别达到0.052μmol·g~(-1)和0.053μmol·g~(-1),与处理1d相比,增加了0.033μmol·g~(-1)和0.027μmol·g~(-1),分别提高了1.74倍和1.04倍。低温处理5 d,宝清红和天津红的产量分别下降了19.39%和41.69%。S_(3307)具有抵御低温的作用,能有效增加红小豆植株茎部可溶性蛋白含量,宝清红和天津红都是在处理3 d时含量达到最高,与喷施清水相比,分别提高了3.45%和3.74%;S_(3307)显著降低MDA含量,处理5 d时,与喷施清水相比,宝清红和天津红分别下降了4.25%和4.55%;同时显著提高POD活性,宝清红和天津红都是在处理3d时活性达到最高,与喷施清水相比,分别提高了3.52%和11.16%。低温处理5 d,喷施S_(3307)提高宝清红和天津红的产量,提高幅度分别为23.06%和43.88%。综上,低温胁迫下,不同品种红小豆茎部逆境生理响应存在显著差异;喷施S_(3307)能够缓解低温胁迫对红小豆的伤害。     

Interactive effects of deficit irrigation and vermicompost on yield,quality, and irrigation water use efficiency of greenhouse cucumber

Water scarcity is the most significant barrier to agricultural development in arid and semi-arid regions. Deficit irrigation is an effective solution for managing agricultural water in these regions. The use of additives such as vermicompost (VC) to improve soil characteristics and increase yield is a popular practice. Despite this, there is still a lack of understanding of the interaction between irrigation water and VC on various crops. This study aimed to investigate the interaction effect of irrigation water and VC on greenhouse cucumber yield, yield components, quality, and irrigation water use efficiency (IWUE). The trials were done in a split-plot design in three replicates in a semi-arid region of southeastern Iran in 2018 and 2019. Three levels of VC in the experiments, i.e., 10 (V₁), 15 (V₂), and 20 t/hm² (V₃), and three levels of irrigation water, i.e., 50% (I₁), 75% (I₂), and 100% (I₃) of crop water requirement were used. The results showed that the amount of irrigation water, VC, and their interaction significantly affected cucumber yield, yield components, quality, and IWUE in both years. Reducing the amount of irrigation water and VC application rates reduced the weight, diameter, length, and cucumber yield. The maximum yield (175 t/hm²) was recorded in full irrigation using 20 t/hm² of VC, while the minimum yield (98 t/hm²) was found in I₁V₁ treatment. The maximum and minimum values of IWUE were recorded for I₁V₃ and I₃V₁ treatments as 36.07 and 19.93 kg/(m³?hm²), respectively. Moreover, reducing irrigation amount decreased chlorophyll a and b, but increased vitamin C. However, the maximum carbohydrate and protein contents were obtained in mild water-stressed conditions (I₂). Although adding VC positively influenced the value of quality traits, no significant difference was observed between V₂ and V₃ treatments. Based on the results, adding VC under full irrigation conditions leads to enhanced yield and IWUE. However, in the case of applying deficit irrigation, adding VC up to a certain level (15 t/hm²) increases yield and IWUE, after which the yield begins to decline. Because of the salinity of VC, using a suitable amount of it is a key point to maximize IWUE and yield when applying a deficit irrigation regime.     

KMnO4和H2O2刺激掘氏疫霉卵孢子萌发对单卵孢株生物学性状的影响

 本文对经KMnO₄、H₂O₂刺激掘氏疫霉(Phytophthora drechsleri)卵孢子萌发建立的单卵孢株群体应用于掘氏疫霉遗传研究的可行性做了研究。2个掘氏疫霉菌株(A₁、A₂交配型各1株)经聚碳膜间隔配对产生的卵孢子用KMnO₄、H₂O₂以及不用上述氧化剂处理分别建立K、H、C 3个单卵孢株群体(卵孢子萌发率8-16%)。在群体水平上对来自同一亲本的上述3个群体生物学性状比较结果表明,H₂O₂处理刺激卵孢子萌发不影响生长速度、菌落形态、耐35℃高温生长、致病力及交配型的遗传稳定性,所建立的单卵孢株群体可用于上述性状的遗传研究;KMnO₄处理导致掘氏疫霉A₁和A₂交配型在自交后代各自分离为A₁、A₂、A₁A₂和A₂、A₁A₂,但不影响其他生物学性状的遗传稳定性,所建立的单卵孢株群体可用于除交配型性状以外的其他生物学性状的遗传研究,不宜用于交配型正常遗传规律的研究。用KMnO₄处理掘氏疫霉A₁或A₂交配型菌株单株自交产生的卵孢子,从A₁菌株自交后代获得A₂和A₁A₂以及从A₂菌株自交后代获得A₁A₂交配型单卵孢株的结果说明,掘氏疫霉异宗配合菌株体内可能同时包含控制A₁和A₂交配型的遗传因子,在正常条件下有1种交配型遗传因子不表达。KMnO₄处理可望作为揭示交配型遗传本质的有用试验手段。     

Low-carbon economic development in Central Asia based on LMDI decomposition and comparative decoupling analyses

Low-carbon economic development is a strategy that is emerging in response to global climate change. Being the third-largest energy base in the world, Central Asia should adopt rational and efficient energy utilization to achieve the sustainable economic development. In this study, the logarithmic mean Divisia index (LMDI) decomposition method was used to explore the influence factors of CO₂ emissions in Central Asia (including Kazakhstan, Uzbekistan, Kyrgyzstan, Tajikistan and Turkmenistan) during the period 1992-2014. Moreover, decoupling elasticity and decoupling index based on the LMDI decomposition results were employed to explore the relationship between economic growth and CO₂ emissions during the study period. Our results show that the total CO₂ emissions decreased during the period 1992-1998, influenced by the collapse of the Soviet Union in 1991 and the subsequent financial crisis. After 1998, the total CO₂ emissions started to increase slowly along with the economic growth after the market economic reform. Energy-related CO₂ emissions increased in Central Asia, mainly driven by economic activity effect and population effect, while energy intensity effect and energy carbon structure effect were the primary factors inhibiting CO₂ emissions. The contribution percentages of these four factors (economic activity effect, population effect, energy intensity effect and energy carbon structure effect) to the total CO₂ emissions were 11.80%, 39.08%, -44.82% and -4.32%, respectively, during the study period. Kazakhstan, Uzbekistan and Turkmenistan released great quantities of CO₂ with the annual average emissions of 189.69×10⁶, 45.55×10⁶ and 115.38×10⁶ t, respectively. In fact, their economic developments depended on high-carbon energies. The decoupling indices clarified the relationship between CO₂ emissions and economic growth, highlighting the occurrence of a 'weak decoupling' between these two variables in Central Asia. In conclusion, our results indicate that CO₂ emissions are still not completely decoupled from economic growth in Central Asia. Based on these results, we suggest four key policy suggestions in this paper to help Central Asia to reduce CO₂ emissions and build a resource-conserving and environment-friendly society.     

窟野河水-气界面CO2交换通量变化特征及其影响因素分析

近年来内陆水体CO₂释放受到广泛关注,为揭示黄土高原地区内陆水体CO₂的释放特征,于2018年7月和10月及2019年3月和6月利用LI-7000 CO₂分析仪对窟野河及代表性水库开展了高频次的水体CO₂分压(pCO₂)和水-气界面CO₂交换通量(FCO₂)观测,并分析其时空变化规律。结果表明:窟野河水体pCO₂和FCO₂(分别为996μatm和94.5 mmol·m^-2·d^-1)均高于水库(分别为752μatm和10.3 mmol·m^-2·d^-1)。FCO₂季节性差异明显:对于河流而言,表现为秋季最高(165.7 mmol·m^-2·d^-1),春季最低(42.9 mmol·m^-2·d^-1);对于水库而言,变化趋势则完全相反,表现为春季最高(16.6 mmol·m^-2·d^-1),秋季最低(-5.4 mmol·m^-2·d^-1)。生物地球化学活性更强的支流FCO₂(107.4 mmol·m^-2·d^-1)高出干流(66.5 mmol·m^-2·d^-1)约50%;同时,位于中下游黄土丘陵区的水库FCO₂(16.4 mmol·m^-2·d^-1)显著高于位于上游呼鄂丘陵区的水库FCO₂(1.2 mmol·m^-2·d^-1)。整体来看,流域水体pCO₂受碳酸盐体系影响最大,有机碳分解作用次之;流速是控制水-气界面气体交换速率的关键因素。在年尺度上,窟野河的河流与水库水体均为大气持续碳源。窟野河平均CO₂释放量与我国长江及国外温带河流相近,但低于黄河中游的其他支流。     

纳米抑菌材料的合成及对茶拟盘多毛孢的抑制活性

茶轮斑病是中国茶园的重要病害，易造成叶片脱落甚至使植株死亡，对茶叶产量和品质造成重大影响。本研究采用组织分离法获得病原菌，通过科赫氏法则验证、形态学观察和分子水平鉴定，确定了病原种类；采用共沉淀法制备了纳米氢氧化镁 [nano-Mg(OH)₂]，采用溶胶-凝胶法制备了纳米二氧化硅 (nano-SiO₂) 和纳米二氧化钛 (nano-TiO₂)，利用X-射线粉末衍射 (XRD) 和扫描电镜 (SEM) 对纳米材料的尺寸和外观形貌进行表征；采用菌丝生长速率法比较了3种纳米材料在质量浓度为25 mg/mL时对茶轮斑病病原菌的抑制活性。结果表明:分离所得菌株与茶拟盘多毛孢Pseudopestalotiopsis theae聚在同一分支中，自检支持率达99%，可以确定引起茶轮斑病的病原菌为茶拟盘多毛孢。所合成的nano-Mg(OH)₂、nano-SiO₂和nano-TiO₂分别呈花瓣状、球状以及聚集状的纳米颗粒，在水中的粒径分别为4342.72、1199.05和654.95 nm，且均具有一定的团聚行为。供试纳米材料对茶拟盘多毛孢的活性高低依次为nano-Mg(OH)₂ > nano-SiO₂ > nano-TiO₂，其中nano-Mg(OH)₂的抑菌效果最佳，150 mg/mL的 nano-Mg(OH)₂施用后3 d抑菌率最高，可达85.14%。研究结果可为茶轮斑病的有效防治提供新的思路和途径。     

预测农药植物角质层-水分配系数的LSER模型

植物角质层-水分配系数(Kcw)对评价农药的渗透及残留具有重要意义。通过实验方法难以高效且经济地测定多种农药的Kcw值,因此有必要发展一种快速预测农药Kcw值的模型。作者收集了23种农药在不同植物中的64个log Kcw实测值,构建了预测农药log Kcw值的线性溶解能关系(liner solvation energy relationship,LSER)模型。所建模型具有良好的拟合度(R^2adj,tra=0.79,RMSEtra=0.38)、稳健性(Q^2BOOT=0.78)和预测能力(Q^2ext=0.81)。该模型可用于预测含有-X(Cl,F,I)、>N-C(O)-NH2、-OCH2COOH、-NH-、-NH2、>C=O、-O-C(O)-NH-、-CN、-S-及-S(O)(O)-等官能团的农药的log Kcw值。机理分析结果表明:疏水相互作用(平均相对贡献率为48%)和n/π电子对相互作用(平均相对贡献率9%)对农药进入植物角质层为正贡献,而氢键受体相互作用(平均相对贡献率26%)和极性相互作用(平均相对贡献率17%)对农药进入角质层为负贡献。本研究构建的pp-LFER模型可用于预测新农药的log Kcw值,并且有助于理解农药在植物角质层与水相间分配的相互作用机制。     

北京地区十字花科蔬菜芜菁花叶病毒株系分化研究

 对北京地区大白菜、不结球白菜、萝卜、甘蓝和花椰菜588份样本进行了病毒种类的鉴定,69.21%的样本感染了芜菁花叶病毒(Turnip mosaic Virus——TuMV)。并对其中33个TuMV分离物按抗病基因型进行了株系分化研究,均获得了台湾省报道的TuMV C₁-C₅ 5个株系。TuMV-C₄占分离物的42.4%,为主要株系,其余依次是C₅、C₁、C₃和C₂株系,这为十字花科蔬菜,特别是大白菜抗TuMV育种提供了依据。     

含1,3,4-噻 (噁) 二唑吩嗪-1-甲酰胺类衍生物的合成与杀菌活性

对广泛存在于链霉菌和铜绿假单胞菌中的一种天然活性物质——申嗪霉素进行了结构修饰,合成了一系列高活性的含1,3,4-噻(噁)二唑的申嗪霉素衍生物 I ₁ ~ I ₂₈ 。杀菌活性测定结果表明:所有目标化合物对禾谷镰刀菌具有较好的杀菌活性,均明显优于母体申嗪霉素。离体杀菌活性测定结果显示,化合物 I ₈ (EC₅₀ = 33.25 μg/mL)和化合物 I ₂₂ (EC₅₀ = 46.52 μg/mL)对禾谷镰刀菌的杀菌活性是申嗪霉素 (EC₅₀ = 128.54 μg/mL)的3~4倍。活体杀菌活性显示,在500 μg/mL质量浓度下,化合物 I ₈ (58.69%)和化合物 I ₂₂ (55.37%)对禾谷镰刀菌的抑制率是申嗪霉素 (25.14%) 的两倍。构效关系分析结果表明,在苯环上引入吸电子基团对化合物的活性不利;而引入给电子基团则有利于提高其杀菌活性。同时,同一取代基在苯环上的取代位置依据活性的高低排列顺序为:邻位>对位>间位。这些结果可用于指导该类化合物的进一步结构改造。     

掘氏疫霉卵孢子萌发研究

 掘氏疫霉(Phytophthora drechsleri)种内菌株直接交配产生的卵孢子分别用0.1% KMnO₄处理20分钟和0.3% H₂O₂处理2分钟在S+L培养基上(26℃)培养7天萌发率>70%。H₂O₂是本研究首次报道的一种刺激掘氏疫霉卵孢子萌发的处理剂,其效果略优于KMnO₄。用KMnO₄和H₂O₂处理均可有效地抑制卵孢子悬浮液中菌丝片段及菌丝膨大体的萌发生长。在一定时期内卵孢子萌发率随卵孢子保存时间的增加而增加,保存30天和45天的卵孢子分别用KMnO₄和H₂O₂处理萌发率最高。卵孢子保存期间有无光照对萌发率无显著影响,但卵孢子荫发时给予光照对萌发有明显的促进作用,保存30天的卵孢子在光照下萌发率为60-70%,在黑暗中仅0-16%。卵孢子萌发过程中的光照条件以黑光灯8小时,日光灯16小时交替连续照射7天效果最好,其次为黑光灯单独连续光照,以日光灯单独照射效果较差。所测定的6种培养基中以S+L培养基对卵孢子萌发的效果最好,其次为V₈+L和WA+L。蜗牛酶、纤维素酶、土壤浸出液和黄瓜果提取液对掘氏疫霉卵孢子萌发无明显刺激作用。     

Effects of mixed-based biochar on water infiltration and evaporation in aeolian sand soil

Aeolian sandy soil in mining areas exhibits intense evaporation and poor water retention capacity. This study was designed to find a suitable biochar application method to improve soil water infiltration and minimize soil water evaporation for aeolian sand soil. Using the indoor soil column method, we studied the effects of three application patterns (A (0-20 cm was a mixed sample of mixed-based biochar and soil), B (0-10 cm was a mixed sample of mixed-based biochar and soil and 10-20 cm was soil), and C (0-10 cm was soil and 10-20 cm was a mixed sample of mixed-based biochar and soil)), four application amounts (0% (control, CK), 1%, 2%, and 4% of mixed-based biochar in dry soil), and two particle sizes (0.05-0.25 mm (S1) and <0.05 mm (S2)) of mixed-based biochar on water infiltration and evaporation of aeolian sandy soil. We separately used five infiltration models (the Philip, Kostiakov, Horton, USDA-NRCS (United States Department of Agriculture-Natural Resources Conservation Service), and Kostiakov-Lewis models) to fit cumulative infiltration and time. Compared with CK, the application of mixed-based biochar significantly reduced cumulative soil water infiltration. Under application patterns A, B, and C, the higher the application amount and the finer the particle size were, the lower the migration speed of the wetting front. With the same application amount, cumulative soil water infiltration under application pattern A was the lowest. Taking infiltration for 10 min as an example, the reductions of cumulative soil water infiltration under the treatments of A2%_(S2), A4%_(S1), A4%_(S2), A1%_(S1), C2%_(S1), and B1%_(S1) were higher than 30%, which met the requirements of loess soil hydraulic parameters suitable for plant growth. The five infiltration models well fitted the effects of the treatments of application pattern C and S1 particle size (R²>0.980), but the R² values of the Horton model exceeded 0.990 for all treatments (except for the treatment B2%_(S2)). Compared with CK, all other treatments reduced cumulative soil water infiltration, except for B4%_(S2). With the same application amount, cumulative soil water evaporation difference between application patterns A and B was small. Treatments of application pattern C and S1 particle size caused a larger reduction in cumulative soil water evaporation. The reductions in cumulative soil water evaporation under the treatments of C4%_(S1), C4%_(S2), C2%_(S1), and C2%_(S2) were over 15.00%. Therefore, applying 2% of mixed-based biochar with S1 particle size to the underlying layer (10-20 cm) could improve soil water infiltration while minimizing soil water evaporation. Moreover, application pattern was the main factor affecting soil water infiltration and evaporation. Further, there were interactions among the three influencing factors in the infiltration process (application amount×particle size with the most important interaction), while there were no interactions among them in the evaporation process. The results of this study could contribute to the rational application of mixed-based biochar in aeolian sandy soil and the resource utilization of urban and agricultural wastes in mining areas.