RESPONSES OF COOL-SEASON GRAIN LEGUMES AND WHEAT TO SOIL-APPLIED ZINC

The yield and zinc (Zn) content response of faba bean (Vicia faba L.), chickpea (Cicer arietinum L.), lentil (Lens culinaris Medik.) and wheat (Triticum aestivum L.) to applications of Zn fertilizer was compared in a glasshouse experiment using two alkaline soils from southwestern Australia. Comparative Zn requirements were determined from yields of 46-day-old dried shoots when no Zn fertilizer was applied, the amount of Zn required to produce the same percentage of the maximum (relative) yield of dried shoots, and the Zn content of dried shoots (Zn concentration multiplied by yield of dried shoots). The concentration of Zn in youngest tissue and in dried shoots was used to determine critical concentrations for Zn in tissue. Faba bean used indigenous soil Zn more effectively than chickpea, followed by wheat and then lentil. The Zn requirement was lowest for faba bean, and increased in the order faba bean < chickpea < wheat < lentil. Zinc concentration in dried youngest tissue and in dried shoots increased with an increase in the amount of added Zn. The critical Zn concentration in the youngest tissue, associated with 90% of the relative yield, was (mg Zn kg^?1): 25 for lentil, 18 for faba bean, 17 for chickpea and 12 for wheat; corresponding values for dried whole tops (mg Zn kg^?1) were: 30 for lentil, 19 for faba bean, 17 for chickpea, and 20 for wheat. Information on comparative responses of the grain legumes to Zn additions relative to wheat, and critical tissue test values, will aid in the fertilizer management of Zn in cool-season grain legumes in the southwestern Australian farming systems.     

Quality,yield and nitrogen fixation of faba bean seeds as affected by sulphur fertilization

Abstract

Faba bean (Vicia faba L.) is an important source of plant protein for humans and animals; however, nutritional value of seeds is notoriously deficient in sulphur (S)-containing amino acids. In this article, the effect of S fertilization on faba bean's capability of N₂ fixation, grain yield and chemical characteristics in terms of protein fractions, fatty acids and minerals composition is reported. A randomized, complete block design with three replicates was used, and three S applications (0, 30 and 60 kg ha^?1, respectively) for faba bean were performed. The S fertilization was split into two applications: 50% before sowing and 50% in the beginning of March as K₂SO₄. At the same time, both the legume and oat crops were fertilized uniformly with 10 kg N ha^?1 as ¹⁵N NH₄ ¹⁵NO₃ (10% ¹⁵N atomic excess) in solution form. In a Mediterranean climate under optimal spring rainfall situations, faba bean produced high yield of grain and protein. Sulphur application resulted in an increase in overall plant yield and N₂ fixation. In addition, S fertilization enhanced the protein quality, increasing its degradable fraction. Fertilizing faba bean with 30 kg ha^?1 of S resulted in a more appropriate dose in order to obtain a quantitative and qualitative crop improvement. From our findings, it can be concluded that S fertilization to faba bean should be recommended to soils with suboptimal S levels to obtain maximum seed and protein yields.     

NITROGEN UPTAKE AND RECOVERY FROM N FERTILIZER AND LEGUME CROPS IN WETLAND RICE MEASURED BY 15N AND NON-ISOTOPE TECHNIQUES

Intensive rice-based cropping systems rely on nitrogenous fertilizer for optimum grain production and legume crops could be used as an alternative nitrogen (N) source for rice. We investigated the fate of N applied to dual cropping wetland rice in the form of legume residue and ¹⁵N labeled fertilizer. In 2001–2002, hairy vetch and broad bean accumulated 131 and 352 kg N ha^?1 of which 41 and 78% was derived from N₂ fixation. In 2002–2003, hairy vetch accumulated 64 kg N ha^?1 and broad bean accumulated 320 kg N ha^?1 of which 21 to 24% was derived from hairy vetch and 31 to 82% N was derived from broad bean by N difference and ¹⁵N-natural abundance method. Our results reveal that hairy vetch and broad bean can supply 50–100% of N required for intensive wetland rice and can be a viable alternative N source to enhance soil fertility.     

Lentil Response to Nitrogen Application and Rhizobia Inoculation

Lentils (Lens culinaris L.) are an important component of the dryland farming systems in the western USA. Optimum nitrogen (N) management can enhance yield and quality of lentils. We conducted a field (at two locations, one with previous history of lentil and the other one without lentil history) and a greenhouse study to evaluate response of lentil to the application of rhizobium inoculant and starter N (control, 22 kg N ha^?1 in the form of urea [U], 22 kg N ha^?1 in the form of slow-release or environmentally safe nitrogen [ESN], and 22 kg N ha^?1 U + 22 kg N ha^?1 ESN). In both, the field and the laboratory studies, lentil yield did not respond positively to the experimental treatments. Lentil average yield was 1216 and 1420 kg ha^?1 at the field condition. In this rain-fed system, lentil yield was mainly limited by moisture availability, and the application of an external N did not contribute to the yield enhancement. Both of these treatments, however, increased protein content. Compared to the control, the application of rhizobium plus U and ESN enhanced protein content by about 34% (from 23.1 to 30.9%). The application of U+ESN also considerably increased postharvest residual nitrate (NO₃)-N in the soil, which can be easily leached and creates environmental pollution. Briefly, the application of U+ESN increases lentil protein content, but more efforts are needed to optimize N management in lentils in order to reduce the environmental concerns in the shallow soil.     

Study on the Effects of Organic and Inorganic Nitrogen Fertilizer on Yield,Yield Components,and Nodulation State of Chickpea (Cicer arietinum L.)

To study the effects of organic and inorganic nitrogen (N) on yield and nodulation of chickpea (Cicer arietinum L.) cv. ILC 482, a spilt-plot experiment based on randomized complete block design with four replications was conducted in 2008 at the experimental farm of the Agriculture Faculty, University of Mohaghegh, Ardabili. Experimental factors were inorganic N fertilizer at four levels (0, 50, 75, and 100 kg ha^?1) in the main plots that applied in the urea form, and two levels of inoculation with Rhizobium bacteria (with and without inoculation) as subplots. Nitrogen application and Rh. inoculation continued to have positive effects on yield and its attributes. The greatest plant height, number of primary and secondary branches, number of pods per plant, number of filled and unfilled pods per plant, number of grains per plant, grain yield, and biological yield were obtained from the greatest level of N fertilizer (100 kg urea ha^?1) and Rh. inoculation. Application of 75 and 100 kg ha^?1 urea showed no significant difference in these traits. Furthermore, the greatest rate of N usage (100 kg urea ha^?1) adversely inhibited nodulation of chickpea. Number and dry weight of nodules per plant decreased significantly with increasing N application rate. The lowest values of these traits recorded in application of 100 kg ha^?1 urea. Results indicated that application of suitable amounts of N fertilizer (i.e., between 50 and 75 kg urea ha^?1) as starter can be beneficial to improve nodulation, growth, and final yield of inoculated chickpea plants.     

Comparing Copper Requirements of Faba Bean,Chickpea, and Lentil with Spring Wheat

Abstract

The copper (Cu) requirement of four crop species was measured in a glasshouse experiment using yield of dried shoots and Cu content (Cu concentration multiplied by yield of dried shoots) of 62 day old plants grown in two different alkaline soils. The species compared were faba bean (Vicia faba L. cv. Fiord), chickpea (Cicer arietinum L. cv. Tyson), lentil (Lens culinaris Medik cv. Digger), and spring wheat (Triticum aestivum L. cv. Stretton). The comparative Cu requirement of the species was determined from yields of dried shoots when no Cu fertilizer was applied, the amount of applied Cu required to produce the same percentage of the maximum (relative) yield of dried shoots, and the Cu content of dried shoots. The concentration of Cu in youngest tissue and in dried shoots was used to determine critical concentrations of Cu in tissue associated with 90% of the maximum yield. Faba bean used indigenous soil Cu more effectively than wheat, followed by chickpea and then lentil. As measured using both shoot yield and Cu content in shoots, the Cu requirement was lowest for faba bean, and increased in the order faba bean < wheat < chickpea < lentil. Copper concentration in dried youngest tissue and in dried shoots increased with an increase in the amount of added Cu. The critical Cu concentration in the youngest tissue was (mg Cu/kg): 4.6 for lentil, 2.6 for chickpea, 1.5 for wheat, and 2.8 for faba bean; corresponding values for dried shoots (mg Cu/kg) were 6.3 for lentil, 3.3 for chickpea, 2.8 for wheat, and 3.0 for faba bean.     

Efficacy of starter N fertilizer and rhizobia inoculant in dry pea (Pisum sativum Linn.) production in a semi-arid temperate environment

ABSTRACT

Uncertainties exist about the importance of rhizobia inoculant and starter nitrogen (N) application in dry pea (Pisum sativum L.) production. Three field experiments were conducted to evaluate how rhizobia inoculant and starter N fertilizer affect pea seed yield and protein concentration in a semi-arid environment in central Montana. Commercial rhizobia inoculant was mixed with seed prior to planting at the manufacturer’s recommended rate. Starter N fertilizers were applied into the same furrow as seed at 0, 22, 44 and 88 kg ha^?1 as urea, slow-release polymer-coated N fertilizer (ESN), and a combination of both. The application of rhizobia inoculant had no or a very small beneficial effect on pea yield in lands with a previous history of peas. In a land without pea history, application of rhizobia increased pea seed yield by 16%. The positive effect of starter N was only pronounced when initial soil N was low (≤ 10 kg ha^?1 nitrate-nitrogen), which increased net return by up to US$ 42 ha^?1. In this condition, application of slow-release N outperformed urea. However, application of starter N (especially with urea) had a negative effect on pea establishment, vigor and seed yield when soil initial N was high (≥ 44 kg ha^?1 NO₃-N). The results indicate that the rate, placement and form of the starter N must be optimized to benefit pea yield and protein without detrimental effects on germination and nodulation. Moreover, application of starter N must be guided by the soil nitrate content.     

Wheat response to N2 fixed by faba bean (Vicia faba L.) as affected by sulfur fertilization and rhizobial inoculation in semi‐arid Northern Ethiopia

Nitrogen fixation in faba bean (Vicia faba cv. Mesay) as affected by sulfur (S) fertilization (30 kg S ha^–1) and inoculation under the semi‐arid conditions of Ethiopia was studied using the ¹⁵N‐isotope dilution method. The effect of faba bean–fixed nitrogen (N) on yield of the subsequent wheat crop (Triticum aestivum L.) was also assessed. Sulfur fertilization and inoculation significantly (p < 0.05) affected nodulation at late flowering stage for both 2004 and 2005 cropping seasons. The nodule number and nodule fresh weighs were increased by 53% and 95%, relative to the control. Similarly, both treatments (S fertilization and inoculants) significantly improved biomass and grain yield of faba bean on average by 2.2 and 1.2 Mg ha^–1. This corresponds to 37% and 50% increases, respectively, relative to the control. Total N and S uptake of grains was significantly higher by 59.6 and 3.3 kg ha^–1, which are 76% and 66% increases, respectively. Sulfur and inoculation enhanced the percentage of N derived from the atmosphere in the whole plant of faba bean from 51% to 73%. This corresponds to N₂ fixation varying from 49 to 147 kg N ha^–1. The percentage of N derived from fertilizer (%Ndff) and soil (%Ndfs) of faba bean varied from 4.3% to 2.8 %, and from 45.1% to 24.0%, corresponding to the average values of 5.1 and 47.9 kg N ha^–1. Similarly, the %Ndff and %Ndfs of the reference crop, barley, varied from 8.5 % to 10.8% and from 91.5% to 89.2%, with average N yields of 9.2 and 84.3 kg N ha^–1. Soil N balance after faba bean ranged from 13 to 52 kg N ha^–1. Beneficial effects of faba bean on yield of a wheat crop grown after faba bean were highly significant, increasing the average grain and N yields of this crop by 1.11 Mg ha^–1 and 30 kg ha^–1, relative to the yield of wheat grown after the reference crop, barley. Thus, it can be concluded that faba bean can be grown as an alternative crop to fallow, benefiting farmers economically and increasing the soil fertility.     

Grain legumes differ in nitrogen accumulation and remobilisation during seed filling

In grain legumes, the N requirements of growing seeds are generally greater than biological nitrogen fixation (BNF) and soil N uptake during seed filling, so that the N previously accumulated in the vegetative tissues needs to be redistributed in order to provide N to the seeds. Chickpea, field bean, pea, and white lupin were harvested at flowering and maturity to compare the relative contribution of BNF, soil N uptake, and N remobilisation to seed N. From flowering to maturity, shoot dry weight increased in all crops by approximately 50%, root did not appreciably change, and nodule decreased by 18%. The amount of plant N increased in all crops, however in field bean (17?g?m^?2) it was about twice that in chickpea, pea, and lupin. The increase was entirely due to seeds, whose N content at maturity was 26?g?m^?2 in field bean and 16?g?m^?2 in chickpea, pea, and lupin. The seed N content at maturity was higher than total N accumulation during grain filling in all crops, and endogenous N previously accumulated in vegetative parts was remobilised to fulfil the N demand of filling seeds. Nitrogen remobilisation ranged from 7?g?m^?2 in chickpea to 9?g?m^?2 in field bean, and was crucial in providing N to the seeds of chickpea, pea, and lupin (half of seed N content) but it was less important in field bean (one-third). All the vegetative organs of the plants underwent N remobilisation: shoots contributed to the N supply of seeds from 58% to 85%, roots from 11% to 37%, and nodules less than 8%. Improving grain legume yield requires either reduced N remobilisation or enhanced N supply, thus, a useful strategy is to select cultivars with high post-anthesis N₂ fixation or add mineral N at flowering.     

COMPARATIVE EFFICIENCY OF BORAX AND GRANUBOR AS BORON FERTILIZERS FOR LENTIL AND SOYBEAN GROWN ON ALLUVIAL ALKALINE SOILS

The two sources of boron (B), i.e., borax and granubor, were evaluated for their efficiency to lentil and soybean grown on alluvium derived soils of Punjab, India. Agronomic efficiency, physiological efficiency, and B recovery were estimated for both the sources and crops. The application of 0.75 kg B ha^-1 through borax and granubor increased lentil seed yield by 21.4 and 23.3%, respectively, over control indicating 2% higher response with granubor application. Boron content in lentil seed increased from 12.2 μg g^-1 in control treatment to the maximum of 24.1 μg g^?1 with the application of 1.25 kg B ha^?1 through granubor. There was 24.6% increase in seed yield of soybean with the application of 1.25 kg B ha^?1 through either of fertilizer source. Total B content increased to maximum of 59.8% over control when B was applied through borax. Apparent B recovery% and recovery efficiency were higher for granubor compared to borax for both the crops.     

Performance of chickpea (Cicer arietinum L.) to bio-fertilizer and nitrogen application in arid condition

Abstract

Field experiments were conducted during 2013–2014 at Tashkent, Uzbekistan to evaluate the performance of chickpea variety “Jakhongir” with the variable proportion of nitrogen (N) and bio-fertilizer inoculation in the moderate saline (5.6?±?0.6?dSm^?1) soil condition. The studied treatments were No control (non-fertilized), N1 mineral-N (50?kg?N?ha^?1), N2, mineral-N (75?kg?N?ha^?1), N3, mineral-N (100?kg?N?ha^?1) equivalent 0%, 50%, 75%, and 100% from recommended rate for chickpea, Rhizobium inoculation (Bio)?+?No control, Rhizobium inoculation (Bio)?+?N1, Rhizobium inoculation (Bio)?+?N2, and Rhizobium inoculation (Bio)?+?N3. Seed inoculation with Rhizobium was significantly superior over no inoculation treatments at all rate of N fertilization. The middle rate of N fertilization 75?kg?N?ha^?1 combined with biofertilizer inoculation had of superior effect on chickpea, producing 73.2% more yield (1.68?Mg ha^?1), oil, protein, and sugar content performed 16.4%; 15.0%, and 17.9% higher value, respectively, in comparison to control.     

Phosphorous and Mung Bean Residue Incorporation Improve Soil Fertility and Crop Productivity in Sorghum and Mungbean-Lentil Cropping System

ABSTRACT

In sorghum and mungbean – lentil cropping system, field experiments were conducted for three successive years to assess the effect of mung bean residue incorporation on sorghum and succeeding lentil productivity along with different doses of phosphorus (P; 0, 30, 60 kg ha^{? 1}) applied to these crops. The level of soil fertility was also tested with or without incorporation of mung bean residue. The interaction of phosphorus to mungbean residue incorporation was thus studied in relation to improve crop productivity with balancing fertilizer requirements through an eco-friendly approach. Sorghum grain yield increased significantly when 60 kg P₂O₅ ha^{? 1} was applied and mungbean residue incorporated. The response was reduced to 30 kg P₂O₅ ha^{? 1} when mungbean residue was not incorporated. The succeeding lentil crop responded up to 60 kg P₂O₅ ha^{? 1} only when preceding sorghum crop received 0 or 30 kg P₂O₅ ha^{? 1}. Response to applied P₂O₅ to lentil reduced to 30 kg ha^{? 1} when preceding sorghum crop received 60 kg P₂O₅ ha^{? 1} and mungbean residue incorporated. Available soil nitrogen, phosphorus, and organic carbon content increased when mungbean residue was incorporated; however, available potassium (K) of the soil decreased from its initial value.     

The inorganic N requirement,nodulation and yield of common bean (Phaseolus vulgaris L.) as influenced by inherent soil fertility of eastern Ethiopia

Though mineral N application impaired nodulation initiation and function, it improves the productivity of common bean. The effect of inorganic application on common bean productivity, however, is dependent on the availability of plant nutrients including nitrogen (N) in the soils. Therefore, multilocation field experiments were conducted at Babillae, Fedis, Haramaya, and Hirna to evaluate the effect of inherent soil fertility status on responsiveness of common bean to different rates of N fertilizer application and its effect on nodulation, yield, and yield components of common bean. The treatments were six levels of N fertilizer (0, 20, 40, 60, 80, and 100 kg N ha^?1) laid out in randomized completed block design with three replications. The result revealed that 20 kg N ha^?1 application significantly improved the nodule number (NN) and nodule dry weight (NDW) except Hirna site, in which reduction of NN and NDW was observed. Although the remaining investigated yield and yield components were significantly improved due to N fertilizer in all study sites, 40 kg N ha^?1 application resulted in significantly increased GY of common bean at Fedis, Haramaya, and Hirna site, while 60 kg N ha^?1 at Babillae site. The highest total biomass yield (7011.6 kg ha^?1) and GY (2475.28 kg ha^?1) of common bean were recorded at Hirna and Haramaya sites, respectively, indicating the importance of better fertile soil for good common bean production. Hence, it can be concluded that the effect of inorganic N on common bean was irrespective of soil fertility rather the total amount of N in soil would affect the need of different rate of inorganic N.     

Impact of variety and farming practices on growth,yield, weed flora and symbiotic nitrogen fixation in faba bean cultivated for fresh seed production

The main objective of this study was to investigate the performance of faba bean landraces originating from different regions of Greece under both organic and conventional farming systems focusing mainly on yield, biological nitrogen fixation (BNF), and competitiveness to weeds. Faba bean exhibited a high ability to fix nitrogen from the atmosphere, as indicated by the percentage of N₂ derived from the atmosphere which exceeded 75% in all evaluated varieties, and the total amount of biologically fixed N up to full anthesis, which fluctuated from 118.5 to 193.9?kg?ha^?1 in the various cropping systems and cultivars. The weed density was appreciably higher in the organic plots, without significant differences among the faba bean cultivars, while wild mustard and corn poppy were the most competitive weeds. The application of inorganic starter fertiliser in the conventionally-treated plots had no negative effect on biologically-fixed nitrogen by faba bean plants, while the herbicide pendimethalin had no negative impact on the nodulation process. Protein concentrations in faba bean cultivars fluctuated from 27.3% to 31.4%. The evaluated landraces could be utilised in breeding programmes due to their earliness, and their high performance in terms of protein content, BNF ability, and productivity.     

Cropping sequence and nitrogen fertilizer effects on the productivity and quality of malting barley and soil fertility in the Ethiopian highlands

The productivity and quality of malting barley were evaluated using factorial combinations of four preceding crops (faba bean, field pea, rapeseed, and barley) as main plots and four nitrogen fertilizer rates (0, 18, 36, and 54 kg N ha^?1) as sub-plots with three replications at two sites on Nitisols of the Ethiopian highlands in 2010 and 2011 cropping seasons. Preceding crops other than barley and N fertilizer significantly improved yield and quality of malting barley. The highest grain yield, kernel plumpness, protein content, and sieve test were obtained for malting barley grown after faba bean, followed by rapeseed and field pea. Nitrogen fertilizer significantly increased yield, protein content, and sieve test of malting barley. All protein contents were within the acceptable range for malting quality. Inclusion of legumes in the rotation also improved soil fertility through increases in soil carbon and nitrogen content. We conclude that to maximize yield and quality of malting barley, it is critical to consider the preceding crop and soil nitrogen status. Use of appropriate break crops may substitute or reduce the amount of mineral N fertilizer required for the production of malting barley at least for one season without affecting its quality.     

Biological nitrogen fixation in faba bean (Vicia faba L.) in the Ethiopian highlands as affected by P fertilization and inoculation

 N₂ fixation by leguminous crops is a relatively low-cost alternative to N fertilizer for small-holder farmers in developing countries. N₂ fixation in faba bean (Vicia faba L.) as affected by P fertilization (0 and 20 kg P ha^–1) and inoculation (uninoculated and inoculated) with Rhizobium leguminosarium biovar viciae (strain S-18) was studied using the ¹⁵N isotope dilution method in the southeastern Ethiopian highlands at three sites differing in soil conditions and length of growing period. Nodulation at the late flowering stage was significantly influenced by P and inoculation only at the location exhibiting the lowest soil P and pH levels. The percentage of N derived from the atmosphere ranged from 66 to 74%, 58 to 74% and 62 to 73% with a corresponding total amount of N₂ fixed ranging from 169 to 210 kg N ha^–1, 139 to 184 kg N ha^–1 and 147 to 174 kg N ha^–1 at Bekoji, Kulumsa and Asasa, respectively. The total N₂ fixed was not significantly affected by P fertilizer or inoculation across all locations, and there was no interaction between the factors. However, at all three locations, N₂ fixation was highly positively correlated with the dry matter production and total N yield of faba bean. Soil N balances after faba bean were positive (12–58 kg N ha^–1) relative to the highly negative N balances (–9–44 kg N ha^–1) following wheat (Triticum aestivum L.), highlighting the importance of rotation with faba bean in the cereal-based cropping systems of Ethiopia. Received: 13 January 2000     

Microbial inoculation of Rhizobium and phosphate-solubilizing bacteria along with inorganic fertilizers for sustainable yield of soybean [Glycine max (L.) Merrill]

A field experiment was conducted to assess the effect of microbial inoculants and inorganic fertilizers for sustaining the yield of soybean. Application of 100% recommended dose of fertilizer (RDF) gave significantly highest yield (2433 kg ha^?1) over 75% RDF (2317 kg ha^?1) and without RDF (2205 kg ha^?1). Seeds inoculated with Rhizobium (Bradyrhizobium japonicum) and phosphate-solubilizing bacteria (2480 kg ha^?1) gave significantly highest soybean yield over without inoculation (2191 kg ha^?1). Rhizobium and phosphate-solubilizing bacteria with 100% RDF (2674 kg ha^?1) gave significantly highest seed yield than rest of the treatment combinations. Root nodules and their dry weight were remained un-influenced due to fertilizer levels, whereas in bio-fertilizers, it was significantly higher with Rhizobium inoculation (24.3 and 408 mg, respectively) followed by dual inoculation of Rhizobium and PSB. 100% RDF and dual inoculation with Rhizobium and PSB earned Rs. 47916/- and Rs. 51182/- net returns per ha, respectively.     

The effect of organic and chemical fertilizers on oilseed rape productivity and weed competition in short rotation

Abstract

A two-year field experiment was conducted to investigate the impact of short crop rotation and organic amendments on rapeseed yield under weed competition conditions. The primary experimental plots consisted of either triticale or pea as a prior crop, consisting of four subplots with either 25 tons of composted cattle manure (CCM), 150?kg urea N ha^?1 (N), 25 tons composted cattle manure + 75?kg urea N ha^?1 (CCM?+?N), or no urea N or manure added as the control (C0). Rapeseed seed yield was not significantly affected by previous crops, except for rapeseed grown after pea which had slightly higher seed yield (2058?kg ha^?1) than those grown after triticale (1942?kg ha^?1). Plants that received CCM?+?N produced the highest amount of seed yield (2447?kg ha^?1), but were not significantly different from plants that received just urea N (2218?kg ha^?1). Weeds gained more biomass when the previous crop was pea compared to those whose previous crop was triticale. Weeds in plots that received CCM?+?N produced the greatest biomass, followed by N, and CCM plots, respectively.     

Effect of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter on their productivity and N turnover in a Vertisol

A long-term field experiment was conducted for 8 years on a Vertisol in central India to assess quantitatively the direct and residual N effects of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter in a soybean–wheat rotation. After cultivation of soybean each year, its aerial residues were removed before growing wheat in the same plots using four N levels (120, 90, 60 and 30 kg ha^?1) and Azotobacter inoculation. Inoculation of soybean increased grain yield by 10.1% (180 kg ha^?1), but the increase in wheat yields with inoculation was only marginal (5.6%; 278 kg ha^?1). There was always a positive balance of soil N after soybean harvest; an average of +28 kg N ha^?1 yr^?1 in control (nodulated by native rhizobia) plots compared with +41 kg N ha^?1 yr^?1 in Rhizobium-inoculated plots. Residual and direct effects of Rhizobium and Azotobacter inoculants caused a fertilizer N credit of 30 kg ha^?1 in wheat. Application of fertilizers or microbial inoculation favoured the proliferation of rhizobia in crop rhizosphere due to better plant growth. Additional N uptake by inoculation was 14.9 kg N ha^?1 by soybean and 20.9 kg N ha^?1 by wheat crop, and a gain of +38.0 kg N ha^?1 yr^?1 to the 0–15 cm soil layer was measured after harvest of wheat. So, total N contribution to crops and soil due to the inoculants was 73.8 kg N ha^?1 yr^?1 after one soybean–wheat rotation. There was a total N benefit of 13.8 kg N ha^?1 yr^?1 to the soil due to regular long-term use of microbial inoculants in soybean–wheat rotation.     

Contribution of Biofertilizers and Fertilizer Nitrogen to Nutrient Uptake and Yield of Egyptian Winter Wheat

ABSTRACT

Nutrient uptake and grain and straw yield of Egyptian winter wheat (Triticum aestivum L. Merr.) were evaluated for two site-years after the seed inoculation with two biofertilizer products, Phosphorien, containing the phosphorus (P)-solubilizing bacteria Bacillus megatherium, and Nitrobien, containing a combination of nitrogen (N)-fixing bacteria Azotobacter chroococcum and Azospirillum liposerum. Ammonium nitrate and polymer-coated urea fertilizers were applied to plots alone and together with the biofertilizers at rates of either 83 kg N ha^?1 or 186 kg N ha^?1 for comparison. The highest grain yield (5.76–6.74 Mg ha^?1) and straw yield (11.49–13.32 Mg ha^?1) occurred at the highest fertilizer rates with N fertilizer. There was a slight additional increase in grain and straw yields when a biofertilizer was applied along with N fertilizer. A slightly higher grain and straw yield was measured with the polymer-coated urea treatment than with the ammonium nitrate treatment. The biofertilizer materials were not as effective as N fertilizers in producing grain (4.02–4.09 Mg ha^?1) or straw (7.71–8.11 Mg ha^?1) for either year, although the Nitrobien + Phosphorien combination increased these parameters over the N-fertilizer control. The effect of the Nitrobien biofertilizer in increasing grain yields was equivalent to a urea application rate of about 13 kg N ha^?1. Biofertilizer inoculations increased iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) concentrations in wheat tissue (at boot stage), but these higher levels did not influence grain or straw yield.