Mulching offers protection from Striga asiatica L. Kutnze parasitism in Sorghum genotypes

The production of sorghum is hampered by the parasitic weed Striga asiatica L. Mulching is promoted as a component of the conservation agriculture systems in sub Saharan Africa. The objective of the study was to assess the effects of mulching and Striga infestation on sorghum chlorophyll content, Striga attachments, sorghum height, and yield. The 2?×?2?×?10 factorial experiment was replicated three times for two seasons. The pot experiment was laid out as a completely randomised design. The first factor was mulching at 0 and 3?tons/ha. The second factor was infestation at two levels: infested and uninfested while the third factor was sorghum varieties at 10 levels. Data collected was plant height, chlorophyll content, Striga counts and sorghum yield. Results indicated that mulching increased chlorophyll content in the second season and not first. Infestation reduced chlorophyll content in both seasons. For the second season, mulching increased chlorophyll content in all genotypes except Ruzangwaya, Mukadziusaende and SC Sila. The genotypes Mukadziusaende, Chiredhi and Hlubi were able to maintain height despite infestation by Striga. Generally yield was higher in uninfested pots across all varieties except Mukadziusaende for the first season. Sorghum arundinaceum was highly susceptible to Striga and hence cannot be a source of resistant traits for Sorghum bicolour breeding. Mulching in Striga infested plants increased plant height and chlorophyll content. Mulching tended to diminish the pathological effects of the Striga parasite in some varieties. Therefore, mulching negated the effect of Striga parasitism and resulted in yield maintenance in some genotypes and hence can be taken up by farmers in Striga infested areas as a beneficial cultural practice.     

Screening of sorghum genotypes for resistance to Striga hermonthica and S. asiatica and compatibility with Fusarium oxysporum f.sp. strigae

In the semi-arid areas of Tanzania, yield losses of sorghum [Sorghum biocolor (L.) Moench] due to Striga hermonthica (Sh) and S. asiatica (Sa) infestations are estimated to be 30–90%. The use of resistant sorghum varieties compatible with Fusarium oxysporum f.sp. strigae (FOS), a biocontrol agent of Striga, may supress the weed and enhance the crop productivity. The objective of this study was to screen and select farmer-preferred sorghum genotypes for Sh and Sa resistance and FOS compatibility for resistance breeding under Tanzanian conditions. Sixty sorghum genotypes were evaluated under screen house conditions using Sh- and Sa-infested field soils with controlled seed infestation, with or without inoculation of the sorghum seeds with FOS. Inoculation of sorghum seeds with FOS significantly enhanced sorghum growth and productivity, and supressed Sh and Sa growth and development. There were reductions of 1–4 Sh and Sa plants when sorghum seeds were inoculated with FOS. Overall, we selected 25 promising sorghum lines resistant to Sh and/or Sa, and with FOS compatibility. The selected sorghum lines are valuable genetic resources for the development of Striga management in sorghum through the integrated use of host resistance and FOS inoculation.     

Genetic analysis of resistance to Fusarium wilt disease,yield and yield-related traits of pigeonpea (Cajanus Cajan (L.) Millsp.)

ABSTRACT

Understanding the genetic control of Fusarium wilt resistance is crucial in pigeonpea breeding programme. This study aimed to study the gene action controlling Fusarium wilt resistance, yield and yield components and select promising crosses possessing resistance to Fusarium wilt along with important agronomic yield traits. Six lines were crossed with four testers in a line x tester mating design. For Fusarium wilt reaction, F₁’s progenies were evaluated in a sick field having inoculums of Fusarium wilt at one location for two seasons and in a pot as well. The F₁’s and parents were evaluated for yield and yield components at two locations for two seasons using a row-column design with two replications. Results indicated that parents and crosses had highly significantly difference for yield and important agronomic traits. General combining ability (GCA) effects of lines and testers were also significant. Specific combining ability (SCA) was also significant for some crosses. Non-additive gene action was more important than additive gene action except for days to maturity and plant height. Crosses ICEAP 00932?×?TZA 2439 and ICEAP 00932?×?TZA 197 displayed small effects of SCA for Fusarium wilt and large SCA for most of yield traits and should be used for integrated disease management.     

Diallel Analysis of Wheat Parents and Their F2 Progenies under Medium and Low Level of Available N in Soil

ABSTRACT

Due to high production cost and prevention of environmental pollution, it is important to reduce the amount of nitrogen (N) fertilizer used on wheat (Triticum aestivum L.). The aim of this study was to evaluate N use of 6 × 6 diallel wheat F₂ progenies and parental lines at low (N0, no N fertilizer) and high (N+, 160 kg N ha^{? 1}) N levels. Significant differences were found between N+ and N0 application in grain N content, grain N yield, N use efficiency for grain N yield (NUEgn), and N use efficiency for grain yield (NUEgy). The cultivar ‘84ÇZT04’ showed positive and high general combining ability (GCA) effects for all traits at low N level. Also, it was the best combiner for all traits at both N levels. The cultivar ‘Genç 99’ was the best parent for GCA effects for grain yield and NUEgy. Hybrids ‘Genç 99 × 84ÇZT04’ and ‘84ÇZT04 × Weaver’ showed positive specific combining ability (SCA) effects for all investigated traits at low and high N levels. Variety ‘84ÇZT04 × Apogee’ had the best SCA effects for all traits at low N level. Overall, the data suggest that it is probable to select promising lines suitable for low N conditions by the crossing of high N use efficient parents.     

Resistance breeding and biocontrol of Striga asiatica (L.) Kuntze in maize: a review

Purpose: The aims of this article are to highlight pre-breeding procedures for identifying primary sources of Striga-resistance genes and to summarize complimentary breeding techniques that enhance partial resistance of maize varieties against Striga species.

Materials and methods: The paper presented a comprehensive account of Striga screening and controlling techniques and highlighted the potential of integrating partial resistance with FOS to boost maize production and productivity in SSA.

Results: Striga infestation is a major constraint to maize production and productivity in Sub-Saharan Africa (SSA). A lack of Striga-resistant maize varieties and the limited adoption of other control methods hinder effective and integrated control of the parasitic weed in maize and related cereal crops globally. Genetic resistance of maize should be complemented with the use of Fusarium oxysporum f.sp. strigea (FOS), a biocontrol agent known to suppress Striga.

Conclusions: A combined use of genetic resistance and FOS has remained largely unutilized in controlling Striga in Africa. A combination of conventional and molecular Striga-resistance breeding tools as well as the use of FOS are promising methods to effectively control Striga in SSA.     

Genotypic Differences in Dry Bean Yield and Yield Components as Influenced by Nitrogen Fertilization and Rhizobia

Dry bean (Phaseolus vulgaris L.) is an important legume worldwide and nitrogen (N) is most yield limiting nutrients. A field experiment was conducted for two consecutive years to evaluate response of 15 dry bean genotypes to nitrogen and rhizobial inoculation. The N and rhizobia treatments were (i) control (0 kg N ha^?1), (ii) seed inoculation with rhizobia strains, (iii) seed inoculation with rhizobia strains + 50 kg N ha^?1, and (iv) 120 kg N ha^?1. Straw yield, grain yield, and yield components were significantly influenced by N and rhizobial treatments. Grain yield, straw yield, number of pods m^?2, and grain harvest index were significantly influenced by year, nitrogen + rhizobium, and genotype treatments. Year × Nitrogen + rhizobium × genotype interactions were also significant for these traits. Hence, these traits varied among genotypes with the variation in year and nitrogen + rhizobium treatments. Inoculation with rhizobium alone did not produce maximum yield and fertilizer N is required in combination with inoculation. Based on grain yield efficiency index, genotypes were classified as efficient, moderately efficient, and inefficient in nitrogen use efficiency (NUE). NUE defined as grain produced per unit N applied decreased with increasing N rate. Overall, NUE was 23.17 kg grain yield kg^?1 N applied at 50 kg N ha^?1 and 13.33 kg grain per kg N applied at 120 kg N ha^?1.     

Characterisation of sweet stem sorghum genotypes for bio-ethanol production

In an effort to characterise and select promising sweet stem sorghum genotypes with enhanced biofuel productivity, the present study investigated phenotypic variability present among diverse sweet stem sorghum genotypes based on ethanol production and related agronomic traits. One hundred and ninety genotypes were evaluated. Data were subjected to variance, cluster, correlation, path coefficient and principal component analyses. Significant differences (P?<?0.01) were detected among tested genotypes for all measured traits. Days to flowering varied from 62 to 152 with a mean of 93. Plant height varied from 90 to 420?cm with a mean of 236?cm. Stem diameter ranged from 7 to 31?mm with a mean of 16?mm. Biomass yield varied from 6.668 to 111.2?t?ha^?1 with a mean of 30?t?ha^?1. Stalk dry matter content ranged from 17.2% to 44.2% with a mean of 29.8%, while fibre content varied from 8.92% to 34.8% with a mean of 17.2%. The stalk brix yield varied from 3.3% to 18.9% with a mean of 12.1%. Ethanol productivity ranged from 240.9 to 5500?l?ha^?1 with a mean of 1886?l?ha^?1. The best ethanol producing genotypes were AS203, AS391, AS205, AS251 and AS448. Days to flowering, plant height, stalk brix and stem diameter exerted the greatest indirect effects on ethanol production through higher biomass production. Biomass yield had the greatest direct effect on ethanol production. Therefore, the above traits should be considered during breeding sorghum for bio-ethanol production. Also, the traits had high heritability values, hence selection should provide for good genetic gains. Overall, the above sweet stem sorghum genotypes are useful genetic resources for breeding of sorghum with enhanced bio-ethanol production.     

Macronutrient accumulation in wheat crop (Triticum aestivum L.) with Azospirillum brasilense associated with nitrogen doses and sources

Abstract

New studies are needed to optimize the nitrogen (N) amount that can be applied to utilize the Azospirillum brasilense benefits. In addition, information regarding the interaction between the urease inhibitor and biological nitrogen fixation (BNF) and how they affect the macronutrients accumulation are also needed. We evaluate the effect of N sources and doses associated with A. brasilense regarding the macronutrients accumulation in straw and grains and wheat grain yield in tropical conditions. A randomized block experimental design was used with four replications in a 2?×?5?×?2 factorial arrangement as follows: two N sources (urea and urea with urease enzyme inhibitor NBPT; five N doses (0, 50, 100, 150, and 200?kg ha^?1) applied in topdressing; with and without A. brasilense inoculation. We found that an increase in N doses positively influenced the accumulation of macronutrients in straw and grains and the wheat grain yield. N sources have similar effects. Inoculation with A. brasilense increased accumulation of Mg and S in straw and P, Ca, and Mg in grains, regardless of the N dose. The inoculation with A. brasilense associated with 140?kg ha^?1 of N increased wheat grain yield. The inoculation can contribute in a more sustainable way to wheat nutrition and optimizing N fertilization.     

Comparative response of spring and winter triticale productivity and bioethanol yield to fertilisation intensity

Grains of triticale are one of the feedstocks suitable for bioethanol production because they are characterised by high starch and low protein contents. In the present study, spring and winter triticale were comparatively studied to evaluate the influence of N fertilisation intensity on the productivity and bioethanol yield, as well as to assess the relationship between the meteorological factors and ethanol yield. Six treatments of N – 0, 60, 90, 120, 150, and 180?kg?ha^?1 were compared in spring triticale and in winter triticale crops. The analysis of variance showed that nitrogen level (factor A), year (factor B) and their interaction (A × B) significantly (P?≤?.01) influenced grain yield, starch yield and bioethanol yield of both spring and winter triticale. Fertilisation was the main factor explaining 47.6% and 41.0% of the total variability of bioethanol yield of spring and winter triticale, respectively. Nitrogen fertiliser rates 120–180?kg?ha^?1 resulted in maximum bioethanol yield of spring triticale (2417–2480?l?ha^?1) and winter triticale (4311–4420?l?ha^?1). Bioethanol conversion efficiency of nitrogen-fertilised spring and winter triticale was similar 492?l?t^?1 and 508?l?^?1, respectively. Meteorological factors had a greater impact on grain productivity and bioethanol yield for winter triticale than for spring triticale. Both seasonal types of triticale could be good feedstocks for bioethanol production in the areas with congenial weather conditions for their cultivation.     

Phenotypic tendency of achene yield and oil contents in sunflower hybrids

ABSTRACT

The knowledge about heterosis breeding of achene yield in sunflower is known; however, dissection of the genetic components of achene yield and seed quality traits is limited. Therefore, the prescribe study was conducted using Line × Tester design to determine combining abilities, and about the maternal-and-paternal gene actions involved in achene yield and seed quality traits in 21 single cross hybrids. Significant mean differences for achene yield and seed quality traits were observed, with total attributed variation (R^2?=^?0.89). Principal component analysis (P?<?0.05) explained 92.6% variation and using factor analysis, we found factor 1 had primary variables (AW, AL, AT, HD, PH, PC, LA and OC) contributed 35.6% variation to achene yield. Combining ability analysis showed the positive general combining ability for E%, HD, PH, 100 AW and AYP in parental inbred lines, whilst, hybrids had positive specific combining ability for E%, HD, PH, 100 AW, DTF, DTM, OA, LA, OC and AYP. Females contributed significantly higher (P?<?0.05) for AYP, E%, HD, NWPH, PH, 100 AW, LA and OA than that of males. Two-way hierarchical clustering showed the most promising hybrid H₅ (A-18?×?G-79) in Cluster V. The hybrid H₅ also showed heterosis, heterobeltosis and commercial heterosis, therefore, genetic exploitation of H₅ is highly desirable in future breeding to map QTLs/genes in arid/semi-arid zones and /or similar growing conditions to boost up yield and seed quality traits.     

Genetic variability and estimates of heritability in sorghum (Sorghum bicolor L.) genotypes grown in a semiarid zone of Sudan

A field experiment was conducted to evaluate nine genotypes of sorghum grown for two consecutive cropping seasons in 2006 and 2007 under rain-fed conditions in a semiarid zone at the Research Farm of El Fasher Research Station, Sudan. The objective of the study was to assess genetic variability and heritability among sorghum genotypes using phenotypic morphological traits. A randomized complete block design with four replications was used for the experiment. The grain yield (kg ha^?1), the number of heads/plant, followed by straw yield (kg ha^?1) had the highest genotypic coefficients of variation in both seasons. High heritability (above 95%) was shown for plant height in both seasons. High genetic advance was reported in straw yield, 1000-grain weight and days to flowering in both seasons. Highly significant differences among genotypes were found for all characters. The high-yielding genotype was Adv-Edo-CWS (E-9) with grain yields of 2780.6 kg ha^?1. Grain yield was significantly and positively correlated with straw yield (r = 0.91), number of heads/plant (r = 0.69), plant height (r = 0.53) and 1000-grain weight (r = 0.36). However, it was significantly and negatively correlated with days to 50% flowering (r = ?0.21). Based on their positive association with grain yield, the character’s straw weight, number of heads/plant, plant height and 1000-grain weight would be the preferable selection criteria for sorghum improvement program in the country.     

Impact of ozone on grain sorghum yield

Grain sorghum (Sorghum vulgare Pers.) is an important animal feed crop, and it is sometimes planted as a substitute for field corn. Although sorghum is grown in areas of the central and southern U.S. where potentially damaging concentrations of O₃ exist, no data are available regarding the sensitivity of grain sorghum to O₃. Plants of grain sorghum (DeKalb A28+ ) were field-grown in open-top chambers and exposed to O₃(7-hr day^?1 seasonal mean concentrations of 0.016, 0.040, 0.059, 0.078, 0.102, and 0.129 ppm) for 85 days to determine the impact of O₃ on grain yield. A randomized complete block design incorporated three replicates of all treatments. Foliar injury was noted at the two highest 03 treatments. Analysis of variance of the data indicated highly significant O₃ effects on overall grain yield. There was a general decrease in yield as O₃ increased, and the overall grain yield reductions were caused primarily by reductions in individual seed weight. Quadratic, Weibull, and plateau-linear models all adequately described the response of grain sorghum to O₃. Yields were not markedly affected at O₃ concentrations below the 0.10 ppm treatment, and the predicted yield loss of 15% at a seasonal 7-hr mean O₃ concentration of 0.13 ppm indicates that grain sorghum exhibits considerable tolerance to O₃.     

Inoculation of rapeseed under different rates of inorganic nitrogen and sulfur fertilizer: impact on water relations,cell membrane stability,chlorophyll content and yield

It is important to develop integrated fertilization strategies for various crops that enhance the competitive ability of the crop, maximize crop production and reduce the risk of nonpoint source pollution from fertilizers. In order to study the effects of mineral nitrogen fertilization and biofertilizer inoculation on yield and some physiological traits of rapeseed (Brassica napus L.) under different levels of sulfur fertilizer, field experiments in factorial scheme based on randomized complete block design were conducted with three replications in 2012 and 2013. Experimental factors were: (1) four levels of chemical nitrogen fertilizer (0, 100, 150 and 200 kg N ha^?1), (2) two levels of biofertilizer (with and without inoculation) consisting Azotobacter sp. and Azospirillum sp. and (3) two levels of sulfur application (0 and 50 kg S ha^?1). Rapeseed yield, oil content of grains and studied physiological traits had a strong association with the N fertilization, biofertilizer inoculation and sulfur (S) application. Higher rates of N fertilization, biofertilizer inoculation and S application increased the grain yield of rapeseed. In the case of physiological traits, the highest value of relative water content (RWC) was recorded in 100 kg N ha^?1 that was statistically in par with 150 kg N ha^?1 application, while usage of 150 kg N ha^?1 showed the maximum cell membrane stability (CMS). Inoculation with biofertilizer and S fertilization resulted in higher RWC and CMS in rapeseed plants. The chlorophyll content showed its maximum values in the highest level of N fertilization, biofertilizer inoculation and S application. The usage of 200 kg N ha^?1 significantly decreased the oil content of rapeseed grains, but the highest grain oil content was obtained from the application of 150 kg N ha^?1, Azotobacter sp. and Azospirillum sp. inoculation and S fertilization. It seems that moderate N rate (about 150 kg N ha^?1) and S application (about 50 kg S ha^?1) can prove to be beneficial in improving growth, development and total yield of inoculated rapeseed plants.     

Effectiveness of native Rhizobium on nodulation and yield of faba bean (Vicia faba L.) in Eastern Ethiopia

This study was initiated to evaluate the effect of locally isolated Rhizobium on nodulation and yield of faba bean at Haramaya, Ethiopia for three consecutive years. Ten treatments comprising of eight effective isolates of rhizobia, uninoculated, and N-fertilized (20 kg N ha^?1) were laid out in a randomized complete block design with three replications. The result of the experiment indicated that all inoculation treatments increased nodule number and dry weight over the control check in all cropping seasons. The result, however, showed the non-significant effect of Rhizobium inoculation on shoot length, number of tiller per plant and 100 seed weight in all cropping season. Inoculating Haramaya University Faba Bean Rhizobium (HUFBR)-15 in 2011 and National Soil Faba Bean Rhizobium (NSFBR)-30 in 2012 and 2013 gave the highest grain yields (4330, 5267 and 4608 kg ha^?1), respectively. These records were 75%, 48%, and 5% over the uninoculated treatment of respective years. Over the season, NSCBR-30 inoculation resulted in the highest nodulation and grain yield production as compared to the other treatments. In general, isolates from central Ethiopia were better than those isolated from eastern Ethiopia and Tropical Agricultural Legume (TAL)-1035 in enhancing faba bean production at Haramaya site. Therefore, NSFBR-30 is recommended as a candidate isolate for faba bean biofertilizer production in eastern Ethiopia soils.     

Enhancing nutrient translocation,yields and water productivity of wheat under rice–wheat cropping system through zinc nutrition and residual effect of green manuring

Abstract

Efficient nutrient and water use are two important considerations to obtain good harvests of wheat. This necessitates the development of an effective nutrient management technique that not only increases yield, but simultaneously can save nutrient and water use. In this context, a field experiment was conducted at Indian Agricultural Research Institute, New Delhi, India to evaluate the residual effect of sesbania and rice bean (in-situ), subabul (ex-situ) green manuring and Zinc (Zn) fertilization, using chelated Zn-ethylenediaminetetraacetic acid (Zn-EDTA) on nutrient use, yields and water productivity of wheat under rice–wheat cropping system. Among residual effects of green manure crops and Zn fertilization, sesbania and foliar spray of 0.5% chelated Zn-EDTA at 20, 40, 60 and 80 days after sowing (DAS) recorded significantly higher nutrient content and uptake and yields than other green manure crops and Zn treatments. Residual effect of sesbania saved about 46.5?×?10³ and 30.5?×?10³ L irrigation water per tonne of wheat over subabul and rice bean, respectively. Foliar spray of 0.5% chelated Zn-EDTA at 20, 40, 60 and 80 DAS saved about 55.5?×?10³, 47?×?10³ and 13?×?10³ L irrigation water per tonne wheat over residual effect of 5?kg Zn ha^?1 through chelated Zn-EDTA as soil application, 2.5?kg Zn ha^?1 through chelated Zn-EDTA as soil application + 1 foliar spray of 0.5% chelated Zn-EDTA at flowering and foliar spray of 0.5% chelated Zn-EDTA at active tillering?+?flowering?+?grain filling, respectively. Correlation analysis showed positive correlation between Zn uptake and grain yield.     

Small-Scale Spatial Variability in Winter Wheat Production

Spatial variability is well documented in agricultural crops. Research has shown that average differences in grain yield for neighboring corn (Zea mays L.) plants can vary by as much as 4211 kg ha^?1; however, little work has been done in winter wheat (Triticum aestivum L.) to determine the amount and scale of spatial variability that exists in grain yields. This study used 22-m?×?0.9-m transects, partitioned in 0.9-m?×?0.9-m subplots, to document the spatial variability that occurred in winter wheat yields. Average yields of each transect ranged from 1023 to 3807 kg ha^?1. Within transects, there was a 1.7- to 2.3-fold difference between the highest and lowest yielding units. This study documented large levels of variability over distances of <1 m. Agronomists working toward precisely managing crop inputs for their most efficient use should account for spatial variability, as significant differences in winter wheat grain yield were found in adjacent 1-m?×?1-m plots.     

Evaluation of yield and some physiological changes in corn and sorghum under irrigation regimes and application of barley residue,zeolite and superabsorbent polymer

In order to investigate the changes in chlorophyll fluorescence, chlorophyll, relative water content (RWC) and forage yield of corn and sorghum under various irrigation regimes and combination treatments of barley residue, zeolite and superabsorbent polymer, an experiment was conducted over 2 years in Kerman, Iran. A randomized complete block design arranged in a factorial split was used with three replications. Two irrigation regimes of normal and drought stress based on 70 and 140 mm cumulative pan evaporation, respectively, and two plant species (corn and sorghum) as factorial combinations were compared in the main plots. Five treatments, (1) 10 t ha^?1 zeolite + 4.5 t ha^?1 residue, (2) 60 kg ha^?1 superabsorbent + 4.5 t ha^?1 residue, (3) 5 t ha^?1 zeolite + 30 kg ha^?1 superabsorbent + 4.5 t ha^?1 residue, (4) 4.5 t ha^?1 residue and (5) – control, were compared in subplots. In both plants, forage yield, potential quantum yield (Fv/Fm), chlorophyll a, total chlorophyll and carotenoid contents decreased significantly under drought stress. Chlorophyll a content, SPAD index and Fv/Fm were higher in corn than in sorghum, but RWC was higher in sorghum. Corn produced higher forage yield (62.8 t ha^?1) than sorghum (49.3 t ha^?1). The application of 10 t ha^?1 zeolite with 4.5 t ha^?1 residue increased most traits more than any of the other treatments, but the superabsorbent had no significant effect on the studied traits.     

Nitrogen acquisition and its relation to growth and yield in recent high-yielding cultivars of rice (Oryza sativa L.) in Japan

During the last two decades, high-yielding cultivars for multipurpose use of rice have been bred and released in Japan. Some of them have repeatedly recorded high yields of over 9?t?ha^?1 of brown rice (about 11.25?t?ha^?1 of rough rice). Here, characteristic features of nitrogen (N) acquisition and its relation to formation of yield components, dry matter production and grain yield at yield levels over 9?t?ha^?1 of brown rice in recent high-yielding cultivars, a large grain type of japonica variety, “Akita 63,” extra-panicle weight types of indica variety, “Takanari” and “Saikai 198,” and a panicle weight type of japonica variety, “Fukuhibiki,” are described as compared with those in the standard japonica cultivars, “Toyonishiki” and “Nipponbare.” The grain yield of the recent high-yielding cultivars was 9.4 to 11.6?t?ha^?1 of brown rice; that is 1.2?1.7 times greater than those of the standard cultivars. Sink capacity (1000-grain weight?×?spikelet number per unit land area) was 47?62% greater in the recent high-yielding cultivars, largely due to their 1.3?1.5 times greater N-use efficiency for sink formation (sink capacity per unit amount of total plant N in the aboveground part at maturity), although major component(s) responsible for their greater sink capacity differ among the cultivars. The ratio of grain yield to total dry matter was 1.1?1.4 times greater in the recent high-yielding cultivars than in the standard cultivars, indicating that the former efficiently translocate dry matter into spikelets during the grain-filling period. N-use efficiency for dry matter production (total dry matter per unit amount of total plant N) was comparable between “Akita 63,” “Fukuhibiki” and “Toyonishiki,” and slightly greater in “Takanari” and “Saikai 198” than in “Nipponbare.”

These results indicate that greater N-use efficiency for sink formation and efficient translocation of dry matter into spikelets contribute greatly to the high-yielding potential of the recent high-yielding cultivars.     

Sources,Rates and Time of Nitrogen Application on Maize Crops under No-Tillage System

Quantitatively, nitrogen (N) is the foremost nutrient for maize crops (Zea mays L.), but the N source to increase the grain productivity still needs more investigation. Thus, the aim of this experiment was to study sources, rates and time of N application on the crop yield and agronomic characteristics of the maize under no-tillage system. The experiment was carried out during two growing seasons on an Oxisol under the factorial 5 × 3 × 3 scheme with five N rates (0, 50, 100, 150, and 200 kg ha^?1) and three sources (ammonium-sulfate-nitrate as inhibitor of the nitrification (ASN+I), ammonium sulfate (AS) and urea); we applied them two times with four replicates: first time at the sowing or later under side dressing when the plants had the six leaves stage. In the first year, the sources of N had no influence on the number of grain line /ear (NGLE), grain number/line (GNL), total number of grain/ear (TNFE), biomass of 100 grain, plant height (PH), height of the first ear insertion (AFEI) and stalk diameter, in contrast with the foliar N content and the crop yield. Early fertilization with N at the sowing time can afford applications as well as the total side dressing. The increase of the rates had positive influence on the N foliar content, plant height and 100 grains biomass. The highest productivities were found with rates above the threshold of 150 kg ha^?1, no matter the sources and the fertilization time.     

Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability

Plant–microorganism associations have long been studied, but their exploitation in agriculture partially or fully replacing chemical fertilizers is still modest. In this study, we evaluated the combined action of rhizobial and plant growth-promoting rhizobacteria inoculants on the yields of soybean and common bean. Seed inoculation with rhizobia (1.2?×?10⁶ cells seed^?1) was compared to co-inoculation with Azospirillum brasilense in-furrow (different doses) or on seeds (1.2?×?10⁵ cells seed^?1) in nine field experiments. The best in-furrow inoculant dose was 2.5?×?10⁵ cells of A. brasilense seed^?1 for both crops. Inoculation with Bradyrhizobium japonicum increased soybean yield by an average 222 kg?ha^?1 (8.4 %), and co-inoculation with A. brasilense in-furrow by an average 427 kg?ha^?1 (16.1 %); inoculation always improved nodulation. Seed co-inoculation with both microorganisms resulted in a mean yield increase of 420 kg?ha^?1 (14.1 %) in soybean relative to the non-inoculated control. For common bean, seed inoculation with Rhizobium tropici increased yield by 98 kg?ha^?1 (8.3 %), while co-inoculation with A. brasilense in-furrow resulted in the impressive increase of 285 kg?ha^?1 (19.6 %). The cheaper, more sustainable inoculated treatment produced yields equivalent to the more expensive non-inoculated + N-fertilizer treatment. The results confirm the feasibility of using rhizobia and azospirilla as inoculants in a broad range of agricultural systems, replacing expensive and environmentally unfriendly N-fertilizers.