山羊链球菌病的防治

<正>随着山羊养殖规模的不断发展壮大,山羊链球菌病的发病率呈逐年上升趋势,对山羊养殖业造成较大的威胁,也给山羊养殖户带来较大的经济损失。现将对山羊链球菌病的防治经验介绍如下,供同行参考。1流行病学山羊链球菌病是由溶血性链球菌或称兽疫链球菌引起的一种急性、热性传染病,主要临床特征     

慢性型羊链球菌病的预防措施与治疗实践

近年来,随着羊肉价格的上涨,羊的饲养量也在逐年增加。羊链球菌病是严重危害山羊、绵羊的疫病,呈点状或局部发生。科学鉴别羊链球菌病,及时对症治疗,有助于促进羊群健康发展,增加养殖效益。     

山羊链球菌病的防治

山羊感染链球菌病后引起败血病,导致体内脏器出血,额下淋巴结肿大、咽喉肿大,具有流行性,山羊生产性能受到影响,并且发病快,死亡率高,严重者24 h内死亡,是制约我国山羊养殖的主要因素之一。正确诊断和预防山羊链球菌病十分重要,本文从山羊链球菌的概念、诊断方法和预防措施3个方面分析了山羊链球菌病,并提出了加强饲养管理、做好选种工作、定期消毒、做好疫苗接种工作等防治措施。     

山羊链球菌病的诊断与防治

山羊链球菌病在养殖过程中时有发生。笔者根据在基层的实践经验,对山羊链球菌病的流行病学、发生症状、病理变化等进行了总结,并介绍了此病的诊断方法和预防治疗措施。     

山羊肺炎链球菌感染的诊断及防治

2017年1月,新疆富蕴县喀拉布勒根乡唐巴勒村某养殖场91只山羊中,先后有24只山羊出现咳嗽、流鼻液、流泪,有的流产,腹泻,在2 d~3 d内死亡。经过临床症状及细菌学检查,确诊为山羊肺炎链球菌病。笔者结合该病例介绍了肺炎链球菌病的诊断及防治方法。     

一起山羊链球菌病的诊断和防治

陕西省凤县唐藏镇隘口村一组,共养山羊375只,7 d内连续死亡32只,发病51只,最多一天死亡12只.经流行病学调查,结合临床症状,剖检变化及细菌分离鉴定,诊断为山羊链球菌病.经预防接种已控制了病情的传播和蔓延.     

山羊败血性脑膜脑炎型链球菌病的诊断与防治

近年来,关于猪的链球菌病报道较多,由链球菌引起的山羊脑膜炎则少见,特将1980年春我旗爆发流行的山羊链球菌病的诊断与防治情况报道如下,供参考: 一、发病情况:1980年4月,内蒙古乌拉特前旗某牧业公社某队的一群山羊280只,于4月3日由     

羊链球菌病的诊断

羊链球菌病是一种急性热性败血性的传染病,主要发生子绵羊,山羊次之。1988年12月三场四连首次暴发成母细毛羊以鼻孔、肛门出血、急性死亡为特征的传染病。通过现场调查、临床症状观察、病理解剖,病原分离培养,生化试验和动物接种等,确诊为羊链球菌病。经用青、链霉素全群预防,取得明显效果,现报告如下。     

山羊链球菌病的诊治

山羊链球菌病过去在我省较少发生，但近年来该病似乎有发展的趋势。由于该病临床症状与山羊传染性胸膜肺炎、羊快疫相似，在诊治上存在一些误区，从而造成较高死亡率，影响了养羊业发展。几年来，我们对福州、宁德、南平等地区山羊发生的链球菌病进行了诊治，获得成功。现将诊治情况报道如下：     

山羊链球菌病的诊治

山羊链球菌病指溶血性链球菌主要通过呼吸道感染山羊,引起山羊的一种急性、败血性传染病.其特征是颌下淋巴结和咽喉肿胀,各脏器出血和胆囊肿大等.     

一株山羊魏氏梭菌的分离鉴定及其致病性研究

本试验从一例病死山羊组织内分离到一株致病性细菌,通过鉴别培养、生化试验、分子生物学试验和动物致病性试验,证明该病原菌为羊致病性A型魏氏梭菌;毒素基因分析结果显示,该菌同时含有α和β2两种毒素基因;动物致病性试验结果表明,该菌对昆明小鼠具有较强的致病性,并从试验致死的昆明小鼠病料中分离到了与病死山羊病原相一致的细菌,从而确定A型魏氏梭菌为引起该山羊死亡的主要病原菌。本试验结果为该羊场魏氏梭菌病的治疗和预防提供了科学依据。     

贵州省山羊源流产细菌调查及致病机理初探

为调查贵州省细菌性山羊流产疫病的主要原因,分析山羊源流产细菌对正常怀孕动物免疫平衡的影响,本试验采集贵州省6个地区(州、市)12个规模养羊场临床健康和发病流产山羊的胎儿、子宫和母羊的阴道棉拭子样本共计107份进行细菌分离和鉴定,采用药敏纸片琼脂扩散法对3种主要羊源分离菌进行20种抗生素的耐药性调查,并用羊源流产细菌建立BALB/c小鼠流产模型,通过ELISA方法对妊娠相关细胞因子和激素进行测定。结果显示,从采集的107份样本中共分离到13个属细菌共302株,其中发病流产山羊生殖器官中分离的细菌种类多于临床健康山羊;流产山羊生殖器官中分离的羊源细菌耐药率高于临床健康山羊细菌耐药率;3种主要羊源流产细菌埃希氏菌、葡萄球菌和链球菌均可引起怀孕BALB/c小鼠发生不同程度的流产,流产率为埃希氏菌>葡萄球菌>链球菌;胚胎吸收率为埃希氏菌>葡萄球菌>链球菌;与空白对照组相比,各试验组BALB/c小鼠血清中的IFN-γ、IL-2含量上升,而IL-4、IL-10和孕酮含量降低。结果表明,贵州省细菌性山羊流产的发生与山羊生殖器官细菌种类多少、耐药性高低有一定相关性,推测山羊源流产细菌可能通过影响怀孕山羊的主要妊娠相关细胞因子和激素的含量导致山羊流产。     

Preliminary Study on Abortion Bacteria from Goat in Guizhou Province and their Pathogenic Mechanism

In order to study the main reason of bacterial goat abortion in Guizhou province,and analyze the bacteria impact of abortion goat immune balance in normal pregnancy animals,107 samples of the goat fetus,uterus and vaginal swabs were collected from clinical health and morbidity abortion goat in 12 scale goat farms of 6 areas in Guizhou province.The bacteria were isolated and identified.The drug susceptibility disc agar diffusion method was launched for the survey of the resistance of three main sources of goat isolates to 20 kinds of antibiotics.The BALB/c mice model of abortion was established using abortion bacteria isolated from goats.The pregnancy related hormones and cytokines were determined by ELISA method.The results showed that 302 strains bacteria of 13 genera were isolated from the samples,and the species of bacteria isolated from morbidity abortion goat were more than the clinical health goats.The bacteria resistant rate of goat reproductive organs in abortion goats was higher than that of clinical healthy goats bacteria.The varying degrees of abortion of BALB/c mice could cause by Escherichia,Staphylococcus and Streptococcus isolated from goats,the rates were Escherichia>Staphylococcus>Streptococcus,respectively and the embryo resorption rates were Escherichia>Staphylococcus>Streptococcus,respectively.Compared with the control group,IFN-γ and IL-2 in the serum were increased;However,IL-4,IL-10 and progesterone were decreased.The results indicated that the occurrence of bacterial goat abortion diseases had certainly related to the number of bacterial types and the level of drug resistance in goat reproductive organs.The levels of pregnancy related hormones and cytokines in goats serum could be changed by abortion bacteria,thus lead to goats abortion.     

Isolation,Identification and Pathogenicity Research of a Strain of Clostridium perfringens from Goat

A pathogenic bacteria was isolated from a dead goat,and it was identified as goat-pathogenic type A Clostridium perfringens by differential culture,biochemical test,molecular biological technology and animal pathogenicity test.Analysis results of toxin genes showed that this bacteria contained both α and β2 toxin genes.Animal pathogenicity test result showed that this bacteria was highly pathogenic to mice,and the same bacteria was isolated from dead mice as which was isolated from the dead goat,so it was certain that type A Clostridium perfringens was the main pathogenic bacteria which caused the goat dead.The results would provide scientific basis for the treatment and prevention of the disease caused by Clostridium perfringens in this goat farm.     

犬用配方羊奶粉对比格幼犬生长性能和肠道菌群的影响

为了探究配方羊奶粉对比格幼犬生长性能与肠道菌群的影响,选取12只15日龄比格犬,随机分为对照组和羊奶粉组,每组6只,对照组幼犬由母犬母乳喂养,羊奶粉组幼犬饲喂足量配方羊奶粉,每3d测量体重并计算体重增长,18 d后采集血样进行血常规、血清生化、免疫因子指标检测,采集粪样进行肠道菌群测序.结果 显示:相比于对照组,羊奶粉...     

三个山羊群体血液蛋白多态性研究

采用垂直平板不连续缓冲系统聚丙烯酰胺凝胶电泳法 ,研究了安哥拉山羊和建昌黑山羊及其高代杂种羊 (F3 ) 9个血液蛋白基因座 (Alb、Pr、Pa、Tf、Hp、Hb、LDH、Akp、Amy)的遗传多态性。结果表明 :安哥拉山羊与F3 血液Alb、Pr、Pa、LDH、Amy呈单态 ,Tf、Hp、Hb、Akp呈多态 ;建昌黑山羊血液Alb、Pr、Pa、Tf、Hp、Hb、LDH、Amy呈单态 ,Akp呈多态。根据所测座位的基因频率计算了群体平均基因杂合度和一致度 ,以及群体间遗传相似系数和遗传距离 ,安哥拉山羊的平均基因杂合度与一致度分别为 0 .1131和 0 .886 9,建昌黑山羊的相应值分别为 0 .0 6 2 1t 0 .9379,F3 则分别为 0 .110 3和0 .8897。安哥拉山羊与F3 的遗传距离小 (0 .0 0 39)、遗传相似系数大 (0 .996 1)。     

DNases in milk and blood sera from different species.

DNases were demonstrated in samples of colostrum and blood serum from man and various domestic animals. The measurable DNase activity recorded was highest in samples from cat and dog and lowest in samples from goat, horse, pig and sheep. In contrast to DNases produced by certain bacteria, these enzymes were thermo-labile and the activity was maximal in the area pH 5.0–5.5.A modification of an agar medium originally described for the demonstration of bacterial DNases was found to be suitable for assays of DNases from colostrum, milk and serum.     

A case of xanthoma in a Saanen goat

This report describes a case of a subcutaneous xanthoma of the sacral region in a 2-year-old female Saanen goat. The tan-coloured mass was 8.5 cm × 4.0 cm × 0.5 cm in size. Yellow-white areas were present across the cut surface. Histopathologically, the mass was composed of foamy macrophages, numerous giant cells, abundant lipid material and cholesterol clefts. The structure consisted of lobular areas surrounded by a stroma. Tissue sections were negative for bacteria, fungi and mycobacteria. Frozen tissue from the mass stained positively with Oil Red O, confirming lipid accumulation in both the extracellular spaces and the large foamy macrophages. Immunohistochemically, the mass was positive for vimentin, proliferating cell nuclear antigen and CD68, but negative for smooth muscle actin, glial fibrillary acidic protein and S100 protein antibodies. As the animal was presented dead, it was not possible to analyse blood lipid levels. To the authors' knowledge, this is the first report of a xanthoma in a goat.     

红枣羊乳啤的制备及其稳定性

为丰富羊乳产品,开阔羊乳市场,给消费者带来更多的消费选择,实验以山羊乳、麦芽、红枣、酒花为主要原料,分别通过乳酸菌的乳酸发酵和酵母菌的酒精发酵后,将得到的红枣羊乳酸液和啤酒原浆按一定体积比配比,制备出兼具酸乳、啤酒、碳酸饮料风味和保健功能的新型红枣羊乳啤饮料,并通过测定沉淀率和稳定系数对其稳定性进行分析。结果表明：当红枣羊乳酸液与啤酒原浆的体积比为1∶1,红枣羊乳酸液中红枣汁添加量12%、羧甲基纤维素钠添加量0.2%、果胶添加量0.15%、蔗糖酯添加量0.1%、白砂糖添加量4%时生产的红枣羊乳啤口感最佳,稳定性最好。