Genome-wide association study of swine farrowing traits. Part II: Bayesian analysis of marker data

Reproductive efficiency has a great impact on the economic success of pork (sus scrofa) production. Number born alive (NBA) and average piglet birth weight (ABW) contribute greatly to reproductive efficiency. To better understand the underlying genetics of birth traits, a genome-wide association study (GWAS) was undertaken. Samples of DNA were collected and tested using the Illumina PorcineSNP60 BeadChip from 1,152 first parity gilts. Traits included total number born (TNB), NBA, number born dead (NBD), number stillborn (NSB), number of mummies (MUM), total litter birth weight (LBW), and ABW. A total of 41,151 SNP were tested using a Bayesian approach. Beginning with the first 5 SNP on SSC1 and ending with the last 5 SNP on the SSCX, SNP were assigned to groups of 5 consecutive SNP by chromosome-position order and analyzed again using a Bayesian approach. From that analysis, 5-SNP groups were selected having no overlap with another 5-SNP groups and no overlap across chromosomes. These selected 5-SNP non-overlapping groups were defined as QTL. Of the available 8,814 QTL, 124 were found to be statistically significant (P < 0.01). Multiple testing was considered using the probability of false positives. Eleven QTL were found for TNB, 3 on SSC1, 3 on SSC4, 1 on SSC13, 1 on SSC14, 2 on SSC15, and 1 on SSC17. Statistical testing for NBA identified 14 QTL, 4 on SSC1, 1 on SSC4, 1 on SSC6, 1 on SSC10, 1on SSC13, 3 on SSC15, and 3 on SSC17. A single NBD QTL was found on SSC11. No QTL were identified for NSB or MUM. Thirty-three QTL were found for LBW, 3 on SSC1, 1 on SSC2, 1 on SSC3, 5 on SSC4, 2 on SSC5, 5 on SSC6, 3 on SSC7, 2 on SSC9, 1 on SSC10, 2 on SSC14, 6 on SSC15, and 2 on SSC17. A total of 65 QTL were found for ABW, 9 on SSC1, 3 on SSC2, 9 on SSC5, 5 on SSC6, 1 on SSC7, 2 on SSC8, 2 on SSC9, 3 on SSC10, 1 on SSC11, 3 on SSC12, 2 on SSC13, 8 on SSC14, 8 on SSC15, 1 on SSC17, and 8 on SSC18. Several candidate genes have been identified that overlap QTL locations among TNB, NBA, NBD, and ABW. These QTL when combined with information on genes found in the same regions should provide useful information that could be used for marker assisted selection, marker assisted management, or genomic selection applications in commercial pig populations.     

Expression of pili and capsule by the avian strain P-1059 of Pasteurella multocida

The avian strain P-1059 of Pasteurella multocida was grown on blood agar (BA), on dextrose-starch agar (DSA), or in Heddleston's hydrogen sulfide test broth. Cells were examined for the presence of pili using electron microscopy after staining with phosphotungstic acid, and they were examined for capsule after ruthenium red staining. Pili were found on the capsulated iridescent type, P-1059I, and on two non-capsulated variants, the blue, P-1059B, and the gray, P-1059G. Many cells grown on BA were heavily piliated. In contrast, fewer cells grown on DSA had pili, and piliation was only slight to moderate. The P-1059I, P-1059B, and P-1059G produced pellicles when grown on broth medium. Pili were found on the circumference of the cells grown on either agar or broth medium. Occasionally a pilus connecting two cells was seen on cells cultured in broth. Cultivation of the P-1059I on DSA containing the iron-chelating agent alpha,alpha'-bipyridyl produced a non-capsulated blue variant. The non-capsulated variant reverted to P-1059I when grown on BA but did not revert when grown on DSA.     

坡向对青藏高原东北缘高寒草甸植被构成和养分特征的影响

坡向是重要地形因子之一,通过影响光照、温度及土壤养分等进而影响群落植物种的组成和分布。本研究通过对青藏高原东北缘不同坡向高寒草甸草地的植被构成和养分特征进行了分析,揭示坡向对植物群落组成和空间分布形成的影响。结果表明,1) 草地群落优势种和亚优势种构成,阳坡以禾本科的赖草、西北针茅和洽草等为主,阴坡以莎草科的矮嵩草和线叶嵩草及豆科的阴山扁宿豆等为主;2)草层高度、植物物种数和α多样性指数为阴坡>阳坡,β多样性指数在坡向之间无显著差异;3) 阳坡和阴坡草地植物生活型以地面芽和地下芽为主,分别占草地群落总物种数的71.0%和89.0%,1/2年生及地上芽植物为阳坡>阴坡,地面芽植物为阴坡>阳坡,地下芽植物在坡向之间无显著差异;4) 草地群落生物量构成,阳坡为禾本科(78.3%)>莎草科(8.3%)>菊科(6.4%)>其他科(7%),阴坡为莎草科(54.3%)>豆科(16.4%)和禾本科(14.8%)>蓼科(5.3%)和菊科(4.6%)>其他科(4.0%);除菊科外,所有各科植物生物量在坡向之间均差异显著,地上总生物量在坡向之间无显著差异;5) 0~20 cm土壤N含量为阴坡>阳坡,10~20 cm土壤P、K、Cu、Zn含量为阳坡>阴坡;6) 0~30 cm土温为阳坡>阴坡,0~40 cm土壤含水量阴坡>阳坡。总之,坡向对高寒草甸草地群落植物种构成和土壤理化特性的变化起着重要作用。     

2015年国外肉牛饲料营养的研究进展

为了全面了解肉牛饲料营养的最新研究进展,作者查阅并总结了2015年关于肉牛饲料营养的国外文献报道,主要从精饲料、粗饲料以及精、粗饲料搭配3个方面进行了综述。精饲料主要包括蛋白质饲料和能量饲料,其中蛋白质饲料的营养研究主要侧重于不同蛋白质饲料对肉牛消化性能、生产性能、酮体特性和肉质以及瘤胃发酵的影响;能量饲料的营养研究侧重于不同类型及加工工艺对肉牛的生产性能、胴体特性以及瘤胃发酵特性的影响;粗饲料的营养研究主要集中于粗料来源及粗料的加工工艺对肉牛生产性能和消化性能两个方面的影响。另外,对精、粗饲料的搭配比例对肉牛生产性能、胴体特性、瘤胃发酵以及采食行为的影响也进行了综述。通过总结和分析肉牛饲料营养的国外研究成果,为中国肉牛业的快速发展提供一定借鉴。     

Influence of footing surface on mounting and other sexual behaviors of estrual Holstein cows

Seven ovariectomized Holstein cows, treated sequentially with progesterone and estradiol benzoate to induce estrus, were used to determine whether cows showed a preference for mounting and displaying other sexual behaviors toward estrual or nonestrual cows tied on dirt or concrete surfaces. Preference tests were conducted in a test area that consisted of equal-sized concrete and dirt surfaces; two cows, designated A and B, were tied on the two surfaces, one on either side. Cows A and B were treated so that on each of four test days both were estrual, only A was estrual, only B was estrual or neither A nor B were estrual. On each test day, five estrual test cows were introduced individually into the test area for two 30-min test periods. During the first test period, tied cow A was on concrete and tied cow B was on dirt, but during the second test period, their positions were switched. Test cows were able to move freely from surface to surface and to interact with tied cow A or B. Estrual test cows spent 21.6 +/- 1.4 min of each 30-min test period on dirt, regardless of the estrual status of the cow tied on concrete or dirt. Mounting activity was 3- to 15-fold greater on dirt than on concrete when there was an estrual cow tied on dirt, regardless of the estrual status of the cow on concrete. Mounting activity was fourfold greater on concrete than on dirt when there was a tied estrual cow on concrete and a tied nonestrual cow on dirt.(ABSTRACT TRUNCATED AT 250 WORDS)     

2013年国外肉牛饲料添加剂研究进展

文章总结分析了国外2013年关于肉牛饲料添加剂的研究成果,分别从脂肪、矿物质、非蛋白氮和饲用微生物等四个方面论述了国外肉牛业饲料添加剂的研究进展.脂肪添加剂主要介绍了脂肪对内牛生产性能和肉质的影响研究,矿物质添加剂介绍了硒、铜、铁、锌等微量元素研究,非蛋白氮和饲用微生物添加剂则分别介绍了尿素和酵母的研究.该研究进展可为我国肉牛产业的快速发展提供一些借鉴.     

Quantitative trait loci mapping for meat quality and muscle fiber traits in a Japanese wild boar x Large White intercross

Three generations of a swine family produced by crossing a Japanese wild boar and three Large White female pigs were used to map QTL for various production traits. Here we report the results of QTL analyses for skeletal muscle fiber composition and meat quality traits based on phenotypic data of 353 F(2) animals and genotypic data of 225 markers covering almost the entire pig genome for all of the F(2) animals as well as their F(1) parents and F(0) grandparents. The results of a genome scan using least squares regression interval mapping provided evidence that QTL (<1% genome-wise error rate) affected the proportion of the number of type IIA muscle fibers on SSC2, the number of type IIB on SSC14, the relative area (RA) of type I on SSCX, the RA of type IIA on SSC6, the RA of type IIB on SSC6 and SSC14, the Minolta a* values of loin on SSC4 and SSC6, the Minolta b* value of loin on SSC15, and the hematin content of the LM on SSC6. Quantitative trait loci (<5% genome-wise error rate) were found for the number of type I on SSC1, SSC14, and SSCX, for the number of type IIA on SSC14, for the number of type IIB on SSC2, for the RA of type IIA on SSC2, for the Minolta b* value of loin on SSC3, for the pH of loin on SSC15, and for the i.m. fat content on SSC15. Twenty-four QTL were detected for 11 traits at the 5% genome-wise level. Some traits were associated with each other, so the 24 QTL were located on 11 genomic regions. In five QTL located on SSC2, SSC6, and SSC14, each wild boar allele had the effect of increasing types I and IIA muscle fibers and decreasing type IIB muscle fibers. These effects are expected to improve meat quality.     

葡萄糖氧化酶对黄羽肉鸡生产性能和养分代谢的影响

本试验研究葡糖糖氧化酶对快大黄羽肉鸡生产性能和养分代谢的影响,选择600只0日龄雏鸡分为A、B、C、D 4个处理,每个处理5个重复,每个重复30只,分别在基础日粮中添加0、250、350、380 g/t葡萄糖氧化酶制剂,测定35、70日龄的生产性能和45日龄的养分代谢率。结果表明,35日龄时,黄羽肉鸡的生产性能不受葡萄糖氧化酶制剂添加的影响;葡萄糖氧化酶制剂对36～70日龄黄羽肉鸡的日增重和料重比有显著影响(P<0.05),D组与B、C组在日增重和料重比上有显著影响(P<0.05);添加葡萄糖氧化酶制剂对45日龄黄羽肉鸡的干物质、有机物质、粗蛋白质、粗脂肪的消化代谢有显著影响(P<0.05)。因此,葡萄糖氧化酶对36～70日龄黄羽肉鸡的日增重、料重比和养分代谢率有显著影响,且在本试验条件下,添加380 g/t葡萄糖氧化酶制剂最适宜。     

A Survey of Gastrointestinal Pig Parasites on Free-range,Organic and Conventional Pig Farms in The Netherlands

The prevalence of gastrointestinal parasites in suckling piglets, weaners, fattening pigs and sows was studied from November 2001 to October 2002 on 16 free-range farms (FRF), 11 organic farms (OF) and 9 conventional farms (CF) by means of faecal examinations of composite samples. Each farm was visited four times with a 3-month interval. Infections with coccidia were found on 43.8% of the FRF, 90.9% of the OF and 66.7% of the CF. Sows had the highest prevalence, particularly on FRF (87.5%) and OF (80%).Ascaris suum was present on 50% of the FRF, 72.7% of the OF and 11.1% of the CF, whereas fattening pigs on FRF (42.9%) and OF (54.5%) had the highest prevalence.Oesophagostomum spp. were observed on 25% of the FRF, 27.2% of the OF and 22.2% of the CF. The infection was most prevalent in the sows on all farm types: 37.5% on FRF, 30% on OF and 22.2% on CF.Trichuris suis was found on 37.5% of the FRF, 36.4% of the OF and 11.1% of the CF. Again, this infection was most prevalent in the sows, particularly on the FRF (50%) and OF (30%). No other gastrointestinal parasite species were found and no clinical signs were observed. No seasonal trends could be distinguished. In many cases, when an age group on a farm was positive for a certain parasite, it remained so during the whole study. This indicates that this parasite was really ‘endemic’ on that farm. The results of this study demonstrate that the prevalence of helminth infections of pigs on farms with outdoor facilities is higher than in pigs on conventional farms.     

Pipeline Impacts and Recovery of Dry Mixed-Grass Prairie Soil and Plant Communities

Agricultural practices have historically dominated disturbance on North American grasslands. Disturbances from oil and gas have become increasingly common and problematic for grassland conservation. With growing demand for oil and gas, industry is actively implementing minimal disturbance techniques during construction to reduce impacts on grasslands. This study aimed to determine impacts of a large-diameter pipeline right of way (ROW) on dry mixed-grass prairie to determine if and how far these impacts extended beyond the ROW and the effect of time on grassland recovery on and off ROW. Soil and vegetation on the ROW and on transects extending 300 m on either side of the ROW were assessed over a 10-yr period, starting the yr of construction, at six sites along a pipeline route in southern Alberta, Canada. There were significant impacts to soil and vegetation on the ROW and within 5 m of the ROW in the first yr. The trench was most impacted, followed by work and storage areas. Within 2 yr, soil and plant communities were on a trajectory toward reference prairie conditions. Ten yr following construction, only soil pH and bare ground were greater, and litter was less, on the trench than on work and storage areas, and relative to reference prairie. While native grass richness, dominance, and cover were similar on and off ROW, abundance of some native forb species was less on ROW. Non-native species cover was < 2% in all yr and locations. Although ruderal weed species were abundant on ROW the yr following construction, they disappeared by the following yr. Use of minimal-disturbance construction techniques reduced the size and intensity of the disturbance footprint, allowing for even sensitive arid habitat to recover within a short period of time. Similar approaches to other grassland disturbances can increase ecosystem resiliency.     

不同抗生素对红色型豌豆蚜生长发育和繁殖的影响

为明确不同抗生素处理方式对红色型豌豆蚜(Acyrthosiphon piusm)生长发育和繁殖的影响,将利福平、盐酸金霉素、氯霉素、盐酸土霉素、青霉素G 钾盐和硫酸链霉素6种抗生素分别进行同时喷洒蚕豆(Vicia faba)植株和蚜虫虫体、只喷洒蚜虫虫体和只喷洒蚕豆植株3种处理,获得了各处理下红色型豌豆蚜发育历期和平均产蚜量生物学参数.结果表明:对其生长发育和繁殖影响最大的抗生素是利福平;影响最大的处理方式是同时喷洒植株和虫体.4种组合处理影响大小顺序为:利福平同时喷洒植株和虫体处理>利福平喷洒植株处理>利福平喷洒虫体处理>土霉素同时喷洒植株和虫体.     

The effects of birdsfoot trefoil (Lotus corniculatus) and chicory (Cichorium intybus) when compared with perennial ryegrass (Lolium perenne) on ovine gastrointestinal parasite development, survival and migration

Two experiments were conducted to investigate the effects of birdsfoot trefoil and chicory on parasitic nematode development, survival and migration when compared with perennial ryegrass. In experiment one, sheep faeces, containing 10,385 Cooperia curticei eggs were added to 25 cm diameter pots containing birdsfoot trefoil, chicory or ryegrass, and the pots maintained under optimal conditions for nematode parasite development. Replicate pots of each forage type were destructively sampled on day 8, 16, 20, 28 and 37 to collect the nematode larvae. When forages were compared on a dry matter basis, by day 16 there were 31% and 19% fewer larvae on birdsfoot trefoil and chicory than on ryegrass, respectively (P<0.01). In the second experiment, replicate 1m(2) field plots of birdsfoot trefoil, chicory and ryegrass were sub-sampled on day 14, 21, 35 and 49 for larval counts following the application of sheep faeces containing 585,000 Teladorsagia circumcincta eggs to each plot on day 0. Results showed there were a minimum of 58% and 63% fewer infective stage parasitic larvae on birdsfoot trefoil and chicory, respectively, compared with ryegrass on day 14 and 35 when forages were compared on a forage dry matter, plot area sampled and leaf area basis (P<0.01). Overall, these results indicate that the number of infective stage larvae on birdsfoot trefoil and chicory pasture was reduced by the effect of their sward structure on the development/survival/migration of ovine parasitic nematodes. These effects may be one of the ways in which these forages may affect parasitic infections in grazing livestock.     

Louse and mite infestation in domestic animals in northern Nigeria

Records of domestic animals brought to the Veterinary Entomology Laboratory for diagnosis of suspected lice and mite infestation over a 10 year period were analysed. From a total of 794 suspected cases, 137 (17.3%) and 247 (31.1%) were positive for lice and mange mites respectively. The most common lice species recorded were Linognathus vituli (66.7%) on cattle, L. ovillus (83.3%) on sheep, Haematopinus suis (100%) on pigs and Menacanthus stramineus (54.5%) on poultry. Other lice species recorded included Haematopinus bovis and Solenopotes capillatus on cattle, Damalinia ovis on sheep, Linognathus stenopsis and Menacanthus stramineus on goats, Goniocotes sp. on a horse, Linognathus setosus and Menacanthus stramineus on dogs, Goniodes gigas, Lipeurus caponis, Menopon gallinae and Chelopistes meleagrides on poultry. The most common mite species were Demodex folliculorum on cattle (96.9%) and on dogs (80.8%), Sarcoptes scabiei on pigs (100%) and Notoedres cati (80.3%) on rabbits. Other mite species included Psoroptes communis, Cheyletiella parasitivorax, Ornithonyssus gallinae and Dermanyssus gallinae.     

Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV

In order to understand the molecular mechanism of host susceptibility differential to highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRSV) infection among three breeds of pig, Tibetan pig,ZangMei Black and Yorkshire,the mRNA level of five reported PRRSV receptor genes (HSPG2,SIGLEC1,CD163,VIM and NMMHC-ⅡA) expressed in lung were investigated on 0,4, 7 and 14 days post infection (dpi) with HP-PRRSV JXA1 isolate.The Real-time RT-PCR results showed that the expression of HSPG2 in Tibetan pig increased significantly on 4 and 14 dpi and decreased significantly on 7 dpi compared with the expression on 0 dpi (P < 0.05), and its expression in ZangMei Black on 14 dpi was significantly higher than that of on 7 dpi (P < 0.05);An increased expression of SIGLEC1 in Tibetan pig was observed on 4 and 14 dpi (P < 0.05), and a decreased expression of the same gene was detected in Yorkshire on 4,7 and 14 dpi (P < 0.05);The expression of CD163 elevated significantly on 14 dpi in both Tibetan pig and ZangMei Black (P < 0.05), however its expression reduced significantly on 7 and 14 dpi in Yorkshire (P < 0.05); The expression of VIM gene was significantly higher on 7 dpi in Tibetan pig and significantly lower on 7 dpi in Yorkshire (P < 0.05); And the expression of NMMHC-ⅡA was significantly higher on 4 dpi in ZangMei Black, which was also significantly higher in Yorkshire on 4 and 14 dpi (P < 0.05).In conclusion, SIGLEC1 and VIM might be important genes to influence the host resistance of Tibetan pig,ZangMei Black and Yorkshire to HP-PRRSV infection.     

A survey on neoplasia in domestic species over a 40-year period from 1935 to 1974 in the Republic of South Africa. IV. Tumours occurring in Equidae

A survey was carried out on the neoplasms of horses, donkeys and mules which are recorded in the registration files of the Section of Pathology of the Veterinary Research Institute, Onderstepoort, in the Republic of South Africa, over a 40-year period from 1935 to 1974. A total of 378 tumours are recorded, 339 of which were in horses, 32 in mules and 7 in donkeys. Sarcoids (38%), squamous cell carcinomas (23,5%), fibromas (8,2%), melanomas (8,0%), papillomas (4,5%), fibrosarcomas (3,4%) and lymphosarcomas (3,0%) accounted for 88,6% of the total. Of the 58 sarcoids for which the site or origin was determined, 46,5% occurred on the head, 32,8% on the chest and abdomen, 19% on the limbs especially below the level of the carpus or hock and 1,7% on the neck. Fifty percent of the 89 squamous cell carcinomas occurred on or around the eyes, especially on the eyelids or nictitating membrane, 23% involved the penis and/or prepuce, while just over 20% arose on the skin. The melanomas involved the skin and eye, whilst papillomas originated primarily on the skin and less frequently on the penis.     

A survey of nematode control measures used by milking goat farmers and of anthelmintic resistance on their farms

Forty-seven milking goat herds, located throughout New Zealand, were surveyed for anthelmintic usage and for gastrointestinal nematodes resistant to anthelmintics. Most farmers (62%) followed a predetermined drenching programme and usually (68%) had a policy of alternating between drench families. Alternation was generally within the farming year. Kids were on average being drenched 12.5 times during their first year (range 2-34). Does aged greater than one year were all being drenched at the same frequency (average 13.4, range 2-34) irrespective of age. On each of the 47 farms, a group of goats was treated with a benzimidazole (BZ) drench (oxfendazole, OFZ, at 4.5 mg/kg on 36 farms and thiabendazole, TBZ at 66 mg/kg on 11 farms); a second group was treated with a "cell membrane depolarizing" (CMD) drench (levamisole, LEV, at 8 mg/kg on 35 farms and morantel citrate, MOR, at 10 mg/kg on 12 farms); a third group remained untreated as controls. Faecal egg counts and larval cultures were done before dosing and one week later. Faecal egg count depressions of <80% were taken as being suggestive of anthelmintic resistance. On this basis resistance was found on 79% of farms surveyed. Resistance to BZs alone occurred on 36% of farms, resistance to CMDs alone on 4% and resistance to both on 38% of farms. On 23% of farms one or other of the drenches was without any apparent effect and on one farm both of the drenches tested failed to reduce the egg counts. Post-drenching larval cultures showed Trichostrongylus, Haemonchus and Ostertagia to be the most prevalent genera remaining after drenching with BZ on 14,11, and 8 farms, respectively. After drenching with LEV Ostertagia was the dominant species on 10 farms, Trichostrongylus on 2 and on one farm the two genera were equally common. Following treatment with MOR Trichostrongylus was the dominant species on 3 farms, Haemonchus on 2 and on one farm Trichostrongylus and Ostertagia were equally common. There was a positive association between drenching frequency and the presence of resistance on farms. Possible interpretations of this association are discussed.     

Poult malabsorption syndrome. II. Pathogenesis of skeletal lesions.

One-day-old poults were placed on litter on which poults had previously developed diarrhea, increased mortality, stunting, and malabsorption. Gross, microscopic, and morphometric evaluations of the proximal tibiotarsal growth plate, along with analysis of plasma calcium, phosphorus, and 25-hydroxyvitamin D3 concentrations, were conducted for 3 weeks to determine the development and character of skeletal lesions. Poults developed enteritis with diarrhea and dehydration. Body weights and shank lengths were significantly decreased. Plasma 25-hydroxyvitamin D3 concentrations were significantly decreased. Plasma calcium was significantly decreased on day 8. Plasma phosphorus concentrations were significantly increased on day 8 and were significantly decreased on days 15, 18, and 22. Growth plates narrowed on days 8 and 11 and expanded on days 15, 18, and 22. The proliferating-prehypertrophy zone significantly decreased in length on days 11, 18, and 22, and significantly increased in length on day 15. The unmineralized hypertrophy zone was significantly increased in length on days 15, 18, and 22. The mineralized hypertrophy zone was significantly decreased in length on all days. Skeletal lesions during the poult malabsorption syndrome evolved from an early osteoporotic lesion associated with hypocalcemia to a rachitic lesion associated with depleted vitamin D and hypophosphatemia.     

氮输入对土壤甲烷产生、氧化和传输过程的影响及其机制

随着人为氮输入的增加,外源氮成为影响土壤甲烷产生、氧化和传输过程的重要因素。土壤甲烷排放受氮素有效性的调节,氮输入会改变土壤初始环境和甲烷排放规律,最终影响甲烷排放量。综述了氮输入对土壤甲烷产生、氧化和传输过程的影响及机制。研究表明,1)氮输入对甲烷排放通量的影响存在促进、抑制和不显著3种情况,这主要是甲烷产生、氧化和传输过程的变化引起的;2)氮输入对甲烷产生过程的影响受产甲烷底物和产甲烷微生物活性的控制,氮输入通过增加土壤有机碳的含量为甲烷产生提供了丰富的底物,同时底物理化性质和植被覆盖度的变化使得这种影响复杂化,氮输入既可促进又可抑制产甲烷菌的活性,并且这种作用受氮形态的影响;3)氮输入对甲烷氧化过程的影响主要是通过刺激或抑制甲烷氧化菌的活性实现的,氮形态的不同也使得这种变化更为复杂;4)氮输入对甲烷传输过程的影响主要受植物通气组织的数量以及传输效率的控制,并且在不同生态系统这种控制作用差异较大。综上所述,氮输入对土壤甲烷产生、氧化和传输过程的影响及机制具有明显的复杂性和不确定性,今后研究中应综合考虑氮输入对甲烷排放关键过程的影响,并侧重于探讨氮输入对相关微生物群落结构、丰度和活性的影响,同时注重对各个生态系统的协同研究,确定氮输入影响下各个生态系统对全球甲烷排放的贡献率。     

Efficacy of netobimin against Muellerius capillaris and resistant strain of digestive tract strongyles in dairy goats

The efficacy of netobimin against digestive tract strongyles and the small lungworm Muellerius capillaris was tested in a dairy goat herd. The drenches were given orally at the rate of 20 mg/kg of body weight once, 10 mg/kg on 2 successive days, or 7.5 mg/kg on 3 successive days. Fecal egg counts and larval counts were done 8 days before and on the day of drenching; further counts were performed on postdrenching days 8 and 18. Two goats were necropsied, 1 on postdrenching day 5 and 1 on day 10 in the group treated on 3 successive days. The fecal egg counts were reduced by 44 to 79% depending on dosage on postdrenching day 18; the remaining worms were Trichostrongylus colubriformis. The larval counts of M capillaris were reduced by 72 to 92%, depending on dose. The reduction was significant in all the treated groups, compared with that in the control group. The use of netobimin at the dosage of 10 mg/kg given on 2 successive days or 7.5 mg/kg given on 3 successive days might be recommended to treat goats infected with Muellerius spp and digestive tract strongyles.     

Leg lesions and cleanliness of finishing bulls kept in housing systems with different lying area surfaces

The influence of the quality of different lying surfaces on lesions and swellings at the joints as well as on the cleanliness of finishing bulls throughout the fattening period was studied. On 17 farms (623 bulls), pens with fully slatted concrete floors (CONCRETE), with rubber coated slats (RUBBER), with cubicles (CUBICLES, provided with five different types of soft lying mat) and with a littered lying area (STRAW) were compared. Bulls kept on STRAW developed the smallest lesion scores at the joints. In CUBICLES, there was a huge variability in the lesion scores depending on the type of lying mat, ranging from values comparable to STRAW up to and greater than the values for CONCRETE. The highest lesion scores at the carpal joints were found on CONCRETE, with intermediate values on RUBBER and in CUBICLES. At the tarsal joints, lesion scores were similar on CONCRETE and RUBBER and in the same range or worse on most mats in the CUBICLES. Swelling scores were highest on CONCRETE and intermediate on RUBBER and in CUBICLES compared to STRAW. In general, there was a steady increase in the lesion scores of the leg joints throughout the fattening period on CONCRETE, RUBBER and STRAW, whereas on some of the mats in CUBICLES these scores were at a high level from early on in the fattening period. Animals in all the housing systems were clean over the whole fattening period. Littering the lying area in CUBICLES affected neither the lesion scores nor the swelling scores at the joints nor animal cleanliness. In conclusion, both rubber coated slats and cubicles provided with soft lying mats were favourable with regard to the levels of lesions and swellings of the leg joints of finishing bulls compared to concrete slats. However, these levels were even lower in pens with a straw bedded lying area.