Relationships between microbial indices in roots and silt loam soils forming a gradient in soil organic matter

Perennial rye grass (Lolium perenne) was grown in a greenhouse pot experiment on seven soils to answer the question whether the microbial colonisation of roots is related to existing differences in soil microbial indices. The soils were similar in texture, but differed considerably in soil organic matter, microbial biomass, and microbial community structure. Ergosterol and fungal glucosamine were significantly interrelated in the root material. This ergosterol was also significantly correlated with the average ergosterol content of bulk and rhizosphere soil. In addition, the sum of fungal C and bacterial C in the root material revealed a significant linear relationship with microbial biomass C in soil. The colonisation of roots with microorganisms increased apparently with an increase in soil microbial biomass. In the root material, microbial tissue consisted of 77% fungi and 23% bacteria. In soil, the fungal dominance was slightly, but significantly lower, with 70% fungi and 30% bacteria. Fungal glucosamine in the root material was significantly correlated with that in soil (r=0.65). This indicates a close relationship between the composition of dead microbial remains in soil and the living fraction in soil and root material for unknown reasons.     

Mineralization of bacteria and fungi in chloroform-fumigated soils

It has been suggested by others that the size of the flush of mineralization caused by CHC1₃ fumigation can be used to estimate the amount of microbial biomass in soils. Calculation of biomass from the flush requires that the proportion of CHCl₃-killed cell C mineralized be known. To determine this proportion, 15 species of [¹⁴C]labelled fungi and 12 species of [¹⁴C]labelled bacteria were added to four types of soil and these were fumigated for 24 h with CHC1₃, reinoculated with unfumigated soil, and incubated at 22°C for 10 days. The average percentage mineralization of the fungi was 43.7 ± 5.3, while the average for the bacteria was 33.3 ± 9.9. Using a 1:3 ratio for distribution of total biomass between the bacterial and fungal populations, respectively, it was calculated that the average mineralization of both types of cells was 41.1%. In experiments conducted to determine if CHC1₃ vapour alters stabilized microbial metabolites or dead microbial cells in a manner which makes them more susceptible to degradation, it was found that both fumigated and unfumigated dead fungal materials mineralized to the same extent in soil during 10 days of incubation.     

The proportional mineralisation of microbial biomass and organic matter caused by air-drying and rewetting of a grassland soil

During the first few days after rewetting of an air-dried soil (AD-RW), microbial activity increases compared to that in the original moist soil, causing increased mineralisation (a flush) of soil organic carbon (C) and other nutrients. The AD-RW flush is believed to be derived from the enhanced mineralisation of both non-biomass soil organic matter (due to its physical release and enhanced availability) and microbial biomass killed during drying and rewetting. Our aim was to determine the effects of AD-RW on the mineralisation of soil organic matter and microbial biomass during and after repeated AD-RW cycles and to quantify their proportions in the CO₂-C flushes that resulted. To do this, a UK grassland soil was amended with ¹⁴C-labelled glucose to label the biomass and then given five AD-RW cycles, each followed by 7 d incubation at 25 °C and 50% water holding capacity. Each AD-RW cycle increased the amount of CO₂-C evolved (varying from 83 to 240 μg g⁻¹ soil), compared to the control with, overall, less CO₂-C being evolved as the number of AD-RW cycles increased. In the first cycle, the amount of biomass C decreased by 44% and microbial ATP by 70% while concentrations of extractable C nearly doubled. However, all rapidly recovered and within 1.3 d after rewetting, biomass C was 87% and ATP was 78% of the initial concentrations measured prior to air-drying. Similarly, by 2 d, extractable organic C had decreased to a similar concentration to the original. After the five AD-RW cycles, the amounts of total and ¹⁴C-labelled biomass C remaining in the soil accounted for 60 and 40% of those in the similarly incubated control soil, respectively. Soil biomass ATP concentrations following the first AD-RW cycle remained remarkably constant (ranging from about 10 to 14 μmol ATP g⁻¹ biomass C) and very similar to the concentration in the fresh soil prior to air-drying. We developed a simple mathematical procedure to estimate the proportion of CO₂-C derived from biomass C and non-biomass C during AD-RW. From it, we estimate that, over the five AD-RW cycles, about 60% of the CO₂-C evolved came from mineralisation of non-biomass organic C and the remainder from the biomass C itself.     

蘑菇生长过程中覆土内微生物群落结构的变化特征

The culturable bacterial population and phospholipid fatty acid (PLFA) profile of casing soil were investigated at different mushroom (Agaricus bisporus) cropping stages. The change in soil bacterial PLFAs was always accompanied by a change in the soil culturable bacterial population in the first flush. Comparatively higher culturable bacterial population and bacterial PLFAs were found in the casing soil at the primordia formation stage of the first flush. There was a significant increase in the ratio of fungal to bacterial PLFAs during mushroom growth. Multivariate analysis of PLFA data demonstrated that the mushroom cropping stage could considerably affect the microbial community structure of the casing soil. The bacterial population increased significantly from casing soil application to the primordia formation stage of the first flush. Casing soil application resulted in an increase in the ratio of gram-negative bacterial PLFAs to gram-positive bacterial PLFAs, suggesting that some gram-negative bacteria might play an important role in mushroom sporophore initiation.     

Soil microbial recolonisation after a fire in a Mediterranean forest

The capacity of different microbial groups to recolonise soil after a fire event will be decisive in determining the microbial community after the fire. Microbial recovery after a wildfire that occurred in Sierra la Grana (Alicante province, southeast Spain) was tracked for 32 months after the fire. Colony forming units (CFUs) of different microbial groups, microbial biomass, soil respiration, bacterial growth (leucine incorporation) and changes in the microbial community structure (phospholipid fatty acid (PLFA) analysis) were determined directly after the fire and four times during the recovery period. Direct effects were reflected by low values of most microbiological variables measured immediately after the fire. Microbial biomass increased during the first year after the fire but was below the unburned reference site 32 months after the fire. Bacterial activity and soil respiration showed the highest values immediately after the fire, but decreased to values similar to that of the unburned reference site or even lower (respiration) 32 months after the fire. Colony forming units of bacterial groups estimated by the plate count method peaked 8 months after the fire, but then decreased, showing values similar to the unburned reference site at the end of the study, with the exception of spore formers, which were 20 times higher than the reference site 32 months after the fire. Fungal CFUs were more sensitive to the fire and recovered more slowly than bacteria. Fungi recovering less rapidly than bacteria were also indicated by the PLFA pattern, with PLFAs indicative of fungi being less common after the fire. The recovery of microbial biomass and activity was mirrored by the initially very high levels of dissolved organic carbon being consumed and decreasing within 8 months after the fire. The wildfire event had thus resulted in a decrease in microbial biomass, with a more bacteria-dominated microbial community.     

退化生态系统植被恢复过程中土壤微生物群落活性响应

通过分析退化生态系统中主要植被恢复类型对土壤微生物群落活性的影响,探讨敏感和可靠的微生物群落活性响应指标,揭示适合当地生态条件的植被恢复类型。结果表明,沙米荒地、白沙蒿、柠条、沙冬青和人工乔木林地土壤微生物量C,N,P和微生物商、蔗糖酶、脲酶、过氧化氢酶、碱性磷酸酶均表现出显著差异(P<0.05)。在土壤各层内,除上层人工乔木林地土壤微生物量N相对较高外,柠条恢复草地土壤微生物量C,N,P都相对较高,沙米荒地均较低;土壤微生物商没有明显的趋势;人工乔木林地蔗糖酶和柠条恢复草地脲酶活性相对较高,过氧化氢酶和碱性磷酸酶活性没有明显的变化趋势,沙米荒地的蔗糖酶、脲酶和过氧化氢酶活性以及白沙蒿草地碱性磷酸酶活性较低。方差分析(ANOVA)显示,蔗糖酶、脲酶、过氧化氢酶和碱性磷酸酶与土壤有机质、全氮以及微生物量C,N,P之间呈显著相关关系;主成分分析(PCA)表明,土壤微生物量N,C,P和蔗糖酶、土壤微生物商基本反映了研究区植被恢复中土壤微生物群落活性的响应信息。不同植被恢复类型草地中土壤微生物群落活性的变化表明,柠条和人工乔木林是研究区域内适合当地生态条件的植被恢复类型。     

Soil microbial community composition as affected by restoration practices in California grassland

Agricultural practices have strong impacts on soil microbes including both the indices related to biomass and activity as well as those related to community composition. In a grassland restoration project in California, where native perennial bunchgrasses were introduced into non-native annual grassland after a period of intensive tillage, weeding, and herbicide use to reduce the annual seed bank, microbial community composition was investigated. Three treatments were compared: annual grassland, bare soil fallow, and restored perennial grassland. Soil profiles down to 80 cm in depth were investigated in four separate layers (0-15, 15-30, 30-60, and 60-80 cm) using both phospholipid ester-linked fatty acid (PLFAs) and ergosterol as biomarkers in addition to microbial biomass C by fumigation extraction. PLFA fingerprinting showed much stronger differences between the tilled bare fallow treatment vs. grasslands, compared to fewer differences between restored perennial grassland and annual grassland. The presence or absence of plants over several years clearly distinguished microbial communities. Microbial communities in lower soil layers were little affected by management practices. Regardless of treatment, soil depth caused a strong gradient of changing habitat conditions, which was reflected in Canonical Correspondence Analysis of PLFAs. Fungal organisms were associated with the presence of plants and/or litter since the total amount and the relative proportion of fungal markers were reduced in the tilled bare fallow and in lower layers of the grassland treatments. Total PLFA and soil microbial biomass were highly correlated, and fungal PLFA biomarkers showed strong correlations to ergosterol content. In conclusion, microbial communities are resilient to the grassland restoration process, but do not reflect the change in plant species composition that occurred after planting native bunchgrasses.     

Soil microorganisms and the growth of Lupinus albus on a high metal soil in the presence of EDTA

A pot experiment was designed with the objective of determining whether the presence of ethylenediaminetetraacetic acid (EDTA) and the resulting mobilization of heavy metals have any affect on: (i) soil microorganisms, (ii) growth of L. albus, and (iii) microbial colonization of roots. There was no effect of the different treatments on the contents of soil microbial biomass C and microbial biomass N. Increasing addition of EDTA to soil led to proportionate increases in extractable C and N, being roughly equivalent to the added amount. Increasing EDTA addition to soil led also to a proportionate increase in mobile heavy metals. Plant height, total amount of shoot and root C were not affected by EDTA addition. Fungal ergosterol in the lupine roots showed a 5- to 8-fold increase in the 0.1 and 0.2% EDTA treatments in comparison with the control. In contrast, EDTA addition did not affect fungal glucosamine or bacterial muramic acid concentrations in the lupine roots. Increasing EDTA addition to soil led also to a proportionate increase in the metal concentrations in the lupine shoots. The concentrations of heavy metals in the lupine shoots and in the NH₄NO₃ soil extracts were all highly significantly correlated.     

Organic Amendment Effects on Microbial Population and Microbial Biomass Carbon in the Rhizosphere Soil of Soybean

The aim of the study was to determine microbial populations and microbial biomass carbon in the rhizosphere soil of soybean cultivated under different organic treatments: plant compost (PC), vermicompost (VER), farmyard manure (FYM), and integrated plant compost (IPC). The serial dilution plate method was employed to enumerate the rhizosphere soil fungi and bacteria. Results showed that microbial populations and biomass carbon were affected by the application of organic amendments. Fungal population was the greatest in the VER plot for two crop cycles, whereas bacterial population was the greatest in the VER in the first crop cycle and FYM for the second crop cycle. Tukey's test at P ≤ 0.05 showed that change in microbial biomass carbon in the sites studied over time was significant, with the greatest in FYM. In our study, addition of organic amendments affected the soil physicochemical properties, which in return affected the rhizosphere microbial characteristics.     

燕沟流域土壤微生物学性质对植被恢复过程的响应

以黄土高原丘陵区陕北延安燕沟流域为例,研究了退耕地土壤微生物生物量对植被恢复过程的响应。结果表明,随植被恢复年限的增加,植被盖度、多度和物种数均呈现先增加后减少然后又增加的趋势。同对照农地相比,表层(0—5 cm)土壤呼吸速率和土壤微生物生物量均明显增加。表层土壤呼吸速率和微生物量碳、氮、磷分别比对照农地增加31.61%3~60.75%和15.19%5~12.81%、122.91%6~97.15%、193.5%2~068.17%。表层土壤呼吸速率对植被恢复的响应是在植被恢复初期(02~9年),随植被恢复年限的增加而增加;植被恢复305~5年期间,随植被恢复年限的增加而减少;而植被恢复55年以后,又随植被恢复年限的增加而增加。植被恢复过程中,土壤微生物量碳、氮和磷的变化与土壤呼吸速率变化趋势类似。除植被恢复8和16年外,植被恢复年限间的土壤呼吸速率、呼吸熵和微生物量碳、氮、磷均有显著性差异。表层土壤呼吸速率与土壤微生物量氮、磷呈极显著相关,土壤微生物量碳与土壤微生物量氮呈显著相关;而土壤呼吸熵与呼吸速率和微生物量碳、氮、磷相关性不明显。     

The influence of tillage on the proportion of organic carbon and nitrogen in the microbial biomass of medium-textured soils in a humid climate

Summary Microbial biomass C and N respond rapidly to changes in tillage and soil management. The ratio of biomass C to total organic C and the ratio of mineral N flush to total N were determined in the surface layer (0–5 cm) of low-clay (8–10%), fine sandy loam, Podzolic soils subjected to a range of reduced tillage (direct drilling, chisel ploughing, shallow tillage) experiments of 3–5 years' duration. Organic matter dynamics in the tillage experiments were compared to long-term conditions in several grassland sites established on the same soil type for 10–40 years. Microbial biomass C levels in the grassland soils, reduced tillage, and mouldboard ploughing treatments were 561, 250, and 155 g g^-1 soil, respectively. In all the systems, microbial biomass C was related to organic C (r=0.86), while the mineral N flush was related to total N (r=0.84). The average proportion of organic C in the biomass of the reduced tillage soils (1.2) was higher than in the ploughed soils (0.8) but similar to that in the grassland soils (1.3). Reduced tillage increased the average ratio of mineral N flush to total soil N to 1.9, compared to 1.3 in the ploughed soils. The same ratio was 1.8 in the grassland soils. Regression analysis of microbial biomass C and percent organic C in the microbial biomass showed a steeper slope for the tillage soils than the grassland sites, indicating that reduced tillage increased the microbial biomass level per unit soil organic C. The proportion of organic matter in the microbial biomass suggests a shift in organic matter equilibrium in the reduced tillage soils towards a rapid, tillage-induced, accumulation of organic matter in the surface layer.     

Effects of long-term 2,4-D application on microbial populations and biochemical processes in cultivated soil

Summary The effects of 15 years of field applications of 2,4-dichlorophenoxy acetate (2,4-D) on soil microbial population and biochemical processes were studied in a field cropped with maize followed by potatoes. Amine or ester formulations at the rate of 0.95 kg 2,4-D per hectare applied in May and October every year. Fungal, bacterial, and actinomycete populations, and microbial biomass C and N were reduced by the 2,4-D treatment, the reduction being more marked where the ester was used. N mineralization, nitrification, and potentially mineralizable N were reduced by the 2,4-D ester only, while urease activity was depressed by both formulations. Dehydrogenase activity and soil microbial respiration tended to be temporarily increased by the amine, but were reduced substantially by the ester, indicating that the ester probably interfered with nutrient cycling.     

Nitrogen turnover in a repeatedly manured arid subtropical soil: Incubation studies with 15N isotopes

Under the hot and moist conditions of irrigated agriculture in the arid subtropics, turnover of organic matter is high, which can lead to considerable carbon (C) and nitrogen (N) losses. Therefore, sustainable use of these soils requires regular manure application at high rates. To investigate the contribution of consecutive manure applications to an arid sandy soil to various soil N pools, goat manure was isotopically labeled by feeding ¹⁵N‐enriched Rhodes grass hay and applied to the soil during a two‐year field experiment. In the first year, soils received ¹⁵N‐labeled manure to distinguish between soil‐derived and manure‐derived N. In the second year, these plots were split for the application of either ¹⁵N‐labeled or unlabeled manure to discriminate N derived from previous (first year) and recent (second year) manure application. Soil samples (of control and ¹⁵N‐manured soil) were collected at the end of the first and the second year, and incubated in two laboratory experiments with labeled or unlabeled manure. At the beginning of Experiment 1, 7% of total N, 11% of K₂SO₄ extractable N, and 16% of microbial biomass N were derived from previously field‐applied manure. While the application of manure during incubation increased microbial biomass N by 225% and 410% in the control soil and the previously field‐manured soil, respectively, N₂O emissions were more affected on the control soil, releasing considerable amounts of the soil N‐pool (80% of total emissions). In Experiment 2, 4% of total N, 7% of K₂SO₄ extractable N, and 7% of microbial biomass N derived from previously applied manure, and 4%, 8%, and 3% from recently applied manure, respectively. Microbial biomass N and N₂O‐N derived from manure declined with time after manure application, whereas in Experiment 1 this tendency was only observed for microbial biomass N.     

Conservation of mineral nitrogen in restored soils at opencast coal mine sites: I. Results from field studies of nitrogen transformations following restoration

One of the characteristics of soils located within the cores of stockpile storage mounds at opencast mine sites is their accumulation of ammonium-nitrogen. Two areas of restored land were constructed from soil stockpiled for 3 years; one consisted of mound-surface (‘aerobic zone’) soil, and the other of deeply buried (‘anaerobic zone’) soil. In that constructed from mound-surface soil, concentrations of both ammonium- and nitrate-nitrogen remained fairly stable throughout the first 6 months of restoration at about 12–20 μg g^?1, but in the site constructed from deeply buried soil, concentrations of ammonium-N decreased from an initial high of 160 to 14 μg N g^?1 soil after 14 weeks, and increased again to 42 μg N g^?1 soil by week 29. In contrast, concentrations of nitrate-nitrogen at the latter site increased from an initial 9 μg to a maximum recorded level of 77 μg N g^?1 soil by week 14, before subsiding to 9 μg N g^?1 soil by week 29. Nitrate was considered to have been lost from the restored soils by a combination of leaching and denitrification, as no vegetation was established at these sites. After a short-term stimulation following restoration, soil microbial biomass levels remained fairly constant, though soils (up to 3 years after restoration) were characterized by a very small ratio of biomass C: organic C.     

不同恢复方式下盐渍化弃耕地土壤生物学活性的变化

以干旱区新疆玛纳斯河流域冲积扇缘定点定位试验地为研究对象, 研究灌溉处理、人工草地处理和补植处理对盐渍化弃耕地土壤微生物量、酶活性及土壤呼吸速率的影响。结果表明, 不同恢复方式均明显增加了土壤微生物数量和土壤微生物量碳、氮及土壤酶活性。不同处理土壤微生物量碳、氮分别比原始弃耕地高17.80%、26.38%、5.33%和7.89%、12.75%、21.93%; 不同处理土壤微生物数量分别是原始弃耕地的4.72倍、6.04倍和4.56倍; 不同处理土壤蔗糖酶活性分别比原始弃耕地高3.4倍、3.2倍和7.7倍, 多酚氧化酶活性比原始弃耕地高1.7倍、1.2倍和1.5倍, 脲酶活性比原始弃耕地高11.1%、52.3%和37.1%; 灌溉处理土壤过氧化氢酶活性最高, 是原始弃耕地的1.53倍, 土壤呼吸速率变化表现为人工草地处理>灌溉处理>补植处理>原始弃耕地, 其中, 人工草地处理土壤呼吸速率比弃耕地高52.25%。相关分析表明, 微生物量碳与微生物C/N和微生物数量之间均呈显著正相关关系(P<0.05); 土壤呼吸速率与土壤脲酶、微生物数量和微生物量碳的相关性达到显著水平(P<0.05), 与土壤微生物量氮呈负相关关系, 但相关性不显著; 土壤蔗糖酶与其他3种酶以及微生物量氮呈显著正相关关系, 土壤脲酶与微生物数量呈显著正相关关系, 多酚氧化酶与过氧化氢酶相关性达到显著水平(P<0.05)。本研究表明干旱区盐渍化弃耕地采用灌溉与人工草地处理有利于土壤养分积累, 可在一定程度上改善土壤质量。     

黄土丘陵区自然植被恢复下土壤微生物学质量演变特征

为了探讨黄土丘陵区自然植被恢复过程中土壤微生物学质量演变特征,用时空互代法对燕沟和县南沟典型小流域自然植被恢复下5个演替阶段（退耕1~6 a、7~17 a、18~35 a、36~60 a、> 60 a）土壤养分含量、微生物量和酶活性进行了研究。结果表明:随着地区植被退耕后自然演替的推进,土壤有机碳、全氮、有效氮与有效钾含量持续增加,土壤全磷含量在不同植被演替阶段变化不明显,有效磷含量在植被演替至多年生草本阶段（18~35 a）时含量最低;土壤细菌约占土壤微生物量的65%左右,且其数量在植被演替至多年生草本阶段时最多,土壤真菌和放线菌随退耕年限的延长呈现不断增加的趋势;土壤碱性磷酸酶、脲酶和蔗糖酶活性也随着植被自然演替的推进呈不断增加趋势,但脲酶和蔗糖酶在植被演替至灌木阶段（36 a）后增速放缓。相关性分析表明,自然恢复过程中土壤微生物数量与酶活性的提高程度比较一致,其与土壤养分关系密切,因此土壤微生物群落与土壤酶活性是反映植被恢复中土壤生物学质量变化的重要指标。     

Fungal and bacterial recolonisation of acid and alkaline forest soils following artificial heat treatments

The direct response and the short-term recolonisation of soil by fungi and bacteria were studied after heat treatments of a humus soil with high carbon content and low pH, and a calcareous soil with lower carbon content and high pH. Heating was administered using a muffle furnace or an autoclave, with different temperatures and times of heat exposure, after which fresh soil (1%) was added as inoculum. Autoclaved soil showed more marked increases in bacterial growth during the recovery phase than oven-heated soil, and the bacterial growth response was more rapid in calcareous than in humus soil. Fungal growth recovered more rapid and reached values higher than the control in humus soil, while it remained low until the end of the study in calcareous soil. Respiration rate showed similar patterns in both soils. Fungal biomass (ergosterol and PLFA 18:2ω6,9) indicated that fungi benefited by autoclaving in humus soil, while they were disfavoured by this treatment in calcareous soil. The sum of bacterial PLFAs did not change due to heating, but some bacterial PLFAs (e.g. cy17:0) increased in both soils. We propose that the community assembly of the microbial communities after heating were mainly driven by pH, in that the high pH soil selected primarily for bacteria and the low pH soil for fungi.     

巴西东北部地区土地退化对土壤微生物量和活性的影响

Land degradation causes great changes in the soil biological properties.The process of degradation may decrease soil microbial biomass and consequently decrease soil microbial activity.The study was conducted out during 2009 and 2010 at the four sites of land under native vegetation(NV),moderately degraded land(LDL),highly degraded land(HDL) and land under restoration for four years(RL) to evaluate changes in soil microbial biomass and activity in lands with different degradation levels in comparison with both land under native vegetation and land under restoration in Northeast Brazil.Soil samples were collected at 0-10 cm depth.Soil organic carbon(SOC),soil microbial biomass C(MBC) and N(MBN),soil respiration(SR),and hydrolysis of fluorescein diacetate(FDA) and dehydrogenase(DHA) activities were analyzed.After two years of evaluation,soil MBC,MBN,FDA and DHA had higher values in the NV,followed by the RL.The decreases of soil microbial biomass and enzyme activities in the degraded lands were approximately 8-10 times as large as those found in the NV.However,after land restoration,the MBC and MBN increased approximately 5-fold and 2-fold,respectively,compared with the HDL.The results showed that land degradation produced a strong decrease in soil microbial biomass.However,land restoration may promote short-and long-term increases in soil microbial biomass.     

施用有机、无机肥后土壤微生物量、固定态铵的变化及其有效性研究

利用¹⁵N示踪技术和盆栽试验研究了施用有机、无机肥料后，土壤微生物量C、N和土壤固定态铵的变化及其生物有效性。结果表明:土壤微生物量C在小麦三叶期时较高，之后随着外界温度的下降，生物量C逐渐下降;小麦返青后又上升，至开花前后达到最高值，为554.9～794.4mg／kg。并以施用稻草和猪粪处理的最高，单施硫铵的最低，以后逐渐降低直至收获。土壤微生物量N的变化与C的变化不太一致，土壤微生物量N在小麦三叶期最高，为40.8～79.0mg／kg，并以施猪粪和稻草处理的最高，对照处理中最低;随着小麦的生长逐渐下降，到小麦开花前后下降到最低点，但至成熟时又有所上升。土壤固定态铵的变化趋势与土壤微生物N的变化趋势相似。施肥后被固定在微生物体内和粘土矿物中的N，在小麦生长期间有很大部分仍能被小麦吸收利用。被固定在微生物体内的硫铵N、稻草N和猪粪N的最大释放率分别为64.7％～84.3％、60.4％～77.1％、59.3％～685％;被固定在粘土矿物中硫铵N的最大释放率为59.5％～76.2％。     

秸秆还田对土壤氮素转化的影响

利用原状土柱田间培养法 ,测定了冬小麦、夏玉米农田土壤氮 (N)素的年净矿化量 ;利用氯仿熏蒸浸提茚三酮反应氮法测定了土壤微生物量氮的数量 ;利用连续流动分析仪测定了土壤表层无机氮的含量。结果表明 ,在冬小麦秸秆覆盖、夏玉米秸秆翻埋的土壤中 ,第 1年土壤氮净矿化量为N 210kg/hm2,第 2年为 179kg/hm2,2年的净矿化量均基本与同期施氮量相当。在秸秆不还田的土壤中 ,第 1年土壤氮净矿化量为N 164kg/hm2,第 2年为248kg/hm2,年际变化较大。翻埋玉米秸秆导致小麦季土壤表层无机氮数量增加 ,引发土壤氮矿化的正激发效应 ;表层覆盖小麦秸秆对玉米季土壤表层无机氮的影响不明显。秸秆还田后 ,每个生育期开始时 ,土壤微生物量氮比不还田土壤的增加 72 %～ 2.34% ,每个生育期结束时增加 34%～ 72%。在实施秸秆还田的最初 2年内 ,土壤微生物量但氮处于动态调整阶段 ,尚未达到新的稳定状态