重庆地区马尾松嫩枝扦插技术研究

研究了生长调节荆的种类和浓度、基质、插条长度、顶芽有无等因素对马尾松嫩枝扦插插条生根率的影响.实验表明:[1]生长调节剂种类和浓度均极显著地影响马尾松插条生根率,且二者对马尾松插条的生根率存在显著的交互影响.采用IBA和GGR处理的插务生根率显著高于采用NAA和IBA+NAA处理的插条.[2]不同长度的插条之间生根率极显著不同,且插条长度与基质之间不存在显著的交互影响,不管在何种基质内均以5～10 cm长度的插条为最优.[3]采用不同基质扦插的插条生根率极显著地不同.[4]带顶芽的插条生根率极显著大于不带顶芽的插条.

Abstract：

Effects of medium,kind and concentration of growth regulators.cutting length and apical bud on cutting rooting percentage of greenwood cuttings of Pinus massoniana were studied.Kinds and concentrations of growth regulators and their interaction were shown to have highly significant effects on the rooting of the cuttings.Rooting percentage of cuttings dipped in IBA and GGR was significantly higher than that of cuttings dipped in NAA and IBA+NAA.Rooting percentage of cuttings with different lengths differed highly significantly and the interaction of cutting length with medium was non-significant statistically.Cuttings 5-10 cm long had the highest rooting percentage regardless of medium.Rooting percentages of cuttings in different media were significantly different.Rooting percentage of cuttings with the apical bud was highly significantly higher than that of cuttings without the apical bud.     

Effect of Different Factors on Rooting Characteristics and Survival of Lonicera japonica Thunb

[Objective] The aim was to explore the effects of different factors on rapid propagation and seedling survival of Lonicera japonica Thunb. [Method] The cuttings of 1, 2 or 3 years old, with different length, were soaked in different hormones at different concentrations for different time and planted at different dates. Finally, their rooting rate and survival rate in different treatments were measured and compared. [Result] The rooting rate and survival rate of one-year old cuttings with length of 10-13 cm and planted on September 20 and October 20, 2012 were relatively higher among all the treatments. Hormone treatment could significantly promote the callus formation and accelerate the rooting of the cuttings, and improve their survival rate. The cuttings treated with 120-200 mg/L ABT1 or NAA for 35-45 min had higher rooting rate and survival rate. Among them, the survival rate of cuttings treated with 160 mg/L ABT1 for 35 min was the highest, up to 92.5%. [Conclusion] The study provided basis for improving the survival rate of L. japonica by cuttage, and for optimizing the rapid propagation technique.     

悬铃木硬枝扦插年龄效应及对α-NAA处理的响应

采集悬铃木不同年龄母树的一年生硬枝进行扦插试验,扦插前用质量分数为0.01和0.05的α-NAA处理2 h,清水为对照,测定POD、PPO、IAAO活力及C/N,探讨其与插穗生根的关系.结果表明:悬铃木硬枝扦插中存在年龄效应;α-NAA能够扭转这种年龄效应、提高多年生插穗的生根能力;随着母树年龄的增加,硬枝插穗中的POD、PPO、IAAO活力及可溶性糖含量、C/N值均呈下降趋势,插穗内可溶性糖含量、C/N值与插穗生根率正相关;三种氧化酶活性呈现规律性变化,不同处理间和各生根阶段3种酶活性呈现差异性,说明在悬铃木扦插生根过程中,不定根的发生发展与3种酶活性有密切联系.

Abstract：

A cutting experiment was done with hardwood cuttings from mother plants of different ages and treated with different concentrations (100 and 500mg/L) of α-NAA for two hours. The activities of POD,PPO and IAAO and the C/N ratio were determined, and their relationship with rooting rate was discussed.The results showed that an age-effect existed in the rooting of hardwood-cuttings of P. orientali; α-NAA could offset the "age-effect" and promote rooting rate of aging mother plants; the activities of POD, PPO and IAAO, soluble sugar content and C/N in the hardwood decreased with increasing age of the mother plant; soluble sugar content and C/N were in significant positive correlation with rooting rate; differences were observed among all the treatments and rooting stages. The above results suggested that the activity of the 3 oxidases studied were closely related to the rooting process of the cuttings of P. orientali.     

澳大利亚特色花卉Sunfan嫩枝扦插快繁研究

探讨了扦插基质、插条部位、插条长度和生长调节剂的种类、浓度及处理时间等对Sunfan生根的影响,结果表明:当基质配比为:细土∶腐殖质∶珍珠岩=1∶2∶1,插条采用当年生8 cm嫩枝梢部枝条,激素处理采用700mg/L的NAA浸蘸插条基部10 s时,插条具有较高的生根率.     

饲草玉米扦插繁殖技术研究(英文)

[Objective] The experiment aimed to provide technical guidance for cutting propagation of forage maize SAUMZI.[Method]The survival situation of cuttings at different stem parts and with different internodes numbers and nutrient basis of cutting growth were studied in this experiment.[Result]The survival of young cutting was high while the rooting rate,germination rate and soluble sugar content of lower part of old stem and young cutting were all high.The rooting rate and survival rate with two nodes were higher than those with one node.The total nitrogen content in young stem and in all stems of two-year-old strain were relatively high while soluble sugar content was positively correlated to germination rate,however,total nitrogen content did not show obvious correlation with rooting rate and germination rate.[Conclusion]The young cutting possessed the highest survival rate and germination rate.     

基于均匀设计优化牛皮杜鹃嫩叶直接再生芽苗及植株再生体系(英文)

[Objective] The experimental was aimed to screen the optimum regeneration shoot induction media and rooting media for tender leaves of Rhododendron chrysanthum Pall.[Method] The tender leaves of Rhododendron chrysanthum Pall were taken as explants to select the optimum bud induction media and rooting media through uniform design and the screening results were verified.[Result] The optimum media for regeneration shoot of Rhododendron chrysanthum Pall contained 1/4 MS,3.70 mg/L ZT, 0.02 mg/L IAA and 1.00 mg/L KT and its induction rate was 95.5% and the rooting media contained modified MS, 0.10 mg/L IAA and 0.07 mg/L NAA and its rooting rate was 98%. [Conclusion] Through this experiment, regeneration systems for regeneration shoot and regenerated plant from tender leaves of Rhododendron chrysanthum Pall were created successfully.     

泥胡菜的组织培养及高效无性系建立(英文)

[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS+BA 0.3 mg/L+2,4-D 1-1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS+AgNO3 1.5 mg/L +BA 0.4 mg/L +NAA 0.1 mg/L. 1/2 MS+IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [Conclusion] This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.     

百蕊草无性系建立与瓶外生根研究(英文)

[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.     

糙叶杜鹃扦插繁殖试验

为保护、发掘糙叶杜鹃资源,加快糙叶杜鹃大量繁育和推广,以糙叶杜鹃半木质化茎段为材料,研究了插条不同枝龄、扦插基质及生长调节剂浓度等对插条生根率的影响.结果表明:扦插基质以泥炭土:腐殖土=1:2为宜,采用2～4年生插条在200 mg/L IBA+100 mg/L NAA混合溶液中浸泡30s的处理获得较高的扦插生根率,生根...     

极度濒危植物百山祖冷杉水培繁殖(英文)

[Objective] The experiment aimed to explore the influences of phytohormones (ABT and IAA) and nutrient solution on rooting of Abies beshanzuensis M.H.Wu by water cultured medium. [Method] The Abies beshanzuensis M.H.Wu were treated by water (CK), 10 mg/L ABT+ water, 10 mg/L IAA+ water, 10 mg/L ABT+ hoagland solution, 10 mg/L IAA+ hoagland solution, then the rooting process was observed and the formation rate of callus, rooting rate, number of rooting, and root length were investigated and analyzed. [Result] ABT and IAA had obvious influences on callus induction, rooting rate and the number of root of Abies beshanzuensis M.H.Wu by water culture, so they were suitable to be used in water propagation of Abies beshanzuensis M.H.Wu. The treatments of phytohormones had no regular influences on the longest root length and average root length. The nutrient solutions would not generate obvious influence on propagation of Abies beshanzuensis M.H.Wu at firstly stage, but they generated influence on root growth after rooting. [Conclusion] The research provided new ideas for propagation of Abies beshanzuensis M.H.Wu, which could make it out of endangerment situation quickly.