Pressurized Hot Water and DTPA‐Sorbitol as Viable Alternatives for Soil Boron Extraction. I. Boron‐Treated Soil Incubation and Efficiency of Extraction

Abstract

Serious challenges associated with hot water extraction, the standard extraction method for water‐soluble boron (B), limit its use in commercial soil‐testing laboratories. Several alternatives to make B testing more practical have been proposed and studied; none of the alternatives have readily replaced the hot water method. Two relatively new, promising B extraction methods are pressurized hot water and DTPA‐Sorbitol. Very little reported work compares B extraction values obtained from the standard hot water extraction method and these two alternative methods. This study was conducted to complete an initial step in validating new procedures—extracting the designated nutrient from fertilized, incubated soils by using standard and alternative extraction methods and comparing the resulting values. The three extraction methods were used to extract B from samples of calcareous sand and silt loam soils and limed, loamy fine sand, all which had been treated with 10 levels of B (0–8 mg kg^?1) and incubated for 7 and 28 days. The amount of B extracted increased as the rate of B application increased with all three soil‐extraction methods. High correlations (r 0.977–0.999) were observed between extractable B and rate of B application with all three procedures. Correlations between the amount of extractable B using hot water extraction and the value obtained with an alternative extraction method were similar for both methods (r=0.89). Hot water generally extracted the least and pressurized hot water the most B regardless of soil type, rate of application, or duration of incubation. This study suggests the more easily used methods of pressurized hot water and DTPA‐Sorbitol could be recommended as replacements to the cumbersome hot water extraction.     

Extraction of Soil Boron for Predicting Its Availability to Plants

Field and greenhouse studies were conducted in Prince Edward Island (P.E.I.) on soybean (Glycine max(L.) Merr.), red clover (Trifolium pratense L.),alfalfa (Medicago sativa L.), and rutabaga (Brassica napobrassica,Mill).Plant B concentrations were compared to soil B extracted by hot-water,0.05 M HC1,1.5 M CH3COOH and 0.01 M CaCl2.The r values for extractable soil B vs.plant B were:hot water (0.67),0.05 M HCl(0.82),1.5M CH3COOH(0.78) and hot 0.01 M CaCl2(0.61).Results of soil B from the 0.05 M HCl extracts were generally found to give the best correlation and linear regression among the four extractants tested for predicting the availability of B to the plants.Overall,the 0.05 M HCl proved to be superior to hot water extraction and is recommended for predicting the available B status of the acid soils of P.E.I.The probability of error with 0.05 M HCL is less since it is shaken for a fixed period of time as opposed to subjective error which could be caused in monitoring the boiling time using hot water.     

Influence of Gyttja on Shoot Growth and Shoot Concentrations of Zinc and Boron of Wheat Cultivars Grown on Zinc‐Deficient and Boron‐Toxic Soil

Abstract

Greenhouse experiments were carried out to study the influence of gyttja, a sedimentary peat, on the shoot dry weight and shoot concentrations of zinc (Zn) and boron (B) in one bread wheat (Triticum aestivum L., cv. Bezostaja) and one durum wheat (Triticum durum L., cv. Kiziltan) cultivar. Plants were grown in a Zn‐deficient (DTPA‐Zn: 0.09 mg kg^?1 soil) and B‐toxic soil (CaCl₂/mannitol‐extractable B: 10.5 mg kg^?1 soil) with (+Zn = 5 mg Zn kg^?1 soil) and without (?Zn = 0) Zn supply for 55 days. Gyttja containing 545 g kg^?1 organic matter was applied to the soil at the rates of 0, 1, 2.5, 5, and 10% (w/w). When Zn and gyttja were not added, plants showed leaf symptoms of Zn deficiency and B toxicity, and had a reduced growth. With increased rates of gyttja application, shoot growth of both cultivars was significantly enhanced under Zn deficiency, but not at sufficient supply of Zn. The adverse effects of Zn deficiency and B toxicity on shoot dry matter production became very minimal at the highest rate of gyttja application. Increases in gyttja application significantly enhanced shoot concentrations of Zn in plants grown without addition of inorganic Zn. In Zn‐sufficient plants, the gyttja application up to 5% (w/w) did not affect Zn concentration in shoots, but at the highest rate of gyttja application there was a clear decrease in shoot Zn concentration. Irrespective of Zn supply, the gyttja application strongly decreased shoot concentration of B in plants, particularly in durum wheat. For example, in Zn‐deficient Kiziltan shoot concentration of B was reduced from 385 mg kg^?1 to 214 mg kg^?1 with an increased gyttja application. The results obtained indicate that gyttja is a useful organic material improving Zn nutrition of plants in Zn‐deficient soils and alleviating adverse effects of B toxicity on plant growth. The beneficial effects of gyttja on plant growth in the Zn‐deficient and B‐toxic soil were discussed in terms of increases in plant available concentration of Zn in soil and reduction of B uptake due to formation of tightly bound complexes of B with gyttja.     

Response of Chickpea Cultivars to Application of Boron in Boron‐Deficient Calcareous Soils

Abstract

This study was conducted to investigate the effects of four boron (B) doses (control, 0 kg B ha^?1; B₁, 1 kg B ha^?1; B₂, 3 kg B ha^?1; and B₃, 6 kg B ha^?1) in soils deficient in available B (0.19 mg B kg^?1) and lime (CaCO₃) content (20.7%) on yield and some yield components of five chickpea (Cicer arietinum L.) genotypes, namely Akçin‐91, Population, Gökçe, ?zmir‐92, and Menemen‐92 in central Anatolian Turkey in the 2002 and 2003 growing seasons. Plant height, pods per plant, grain yield, protein content, protein yield, thousand seed weight, and leaf B concentration were measured. Grain yields in all genotypes (except for Gökçe) were significantly increased by 1 kg ha^?1 B application. Application of 1 kg ha^?1 B increased the yield by an average of 5%. Genotypes studied showed significant variations with respect to their responses to additional B. Akçin‐91 gave the highest grain yield (1704.8 kg ha^?1) at 3 kg B ha^?1, whereas Population, ?zmir‐92, and Menemen‐92 yielded best (1468.2 kg ha^?1, 1483.0 kg ha^?1, and 1484.7 kg ha^?1, respectively) at 1 kg B ha^?1. Interestingly, Gökçe reached to the highest level of grain yield (1827.1 kg ha^?1) at the control. Gökçe was a B deficiency B tolerance genotype. The other genotypes appeared to have high sensitivity to B deficiency. This study showed that B deficiency could result in significant yield losses in chickpea under the experimental conditions tested. Thus, B contents of soils for the cultivation of chickpea should be analyzed in advance to avoid yield losses.     

Effect of the Extraction Period on the Routine Assessment of Soil P Status by Ion‐Exchange Resin Membranes

The cation–anion exchange resin membrane (CAERM) system is one of the most promising alternative methodologies for the assessment of soil phosphorus (P) availability. Nevertheless, the CAERM method has a recommended period of extraction (16 h) that limits its applicability. With the aim to improve the use of CAERM in routine laboratory work, seven extraction times were studied and compared for repeatability and biological accuracy, using 150 samples of soils and covering a wide range of properties. The results showed dissimilar quantities of extractable P, according to the nature of soils and the period of extraction time. On average of all soils, values of extractable P ranged from a 2.4 mg kg^?1 and 26.7 mg kg^?1 in 0.25‐h and 16‐h extraction times, respectively. Relative to the repeatability aspects, the results reveal a tendency of increasing results with the decrease of the period of extraction, showing coefficient variation (CV) values ranging from 5% (16 h) to 14% (0.25 h). All the extraction periods tested for the CAERM presented highly significant values of r (0.824–0.943) between extractable P and each of the three biological parameters: relative yield of ryegrass, P content, and P uptake. Considering the three biological parameters simultaneously, the best results were obtained for extraction periods of 8 h, 2 h, and 4 h. Based on the results, and considering both practical and accuracy aspects, it is conclude that 2 h of extraction is a valid alternative to the 16‐h standard extraction period for the CAERM methodology.     

Bioavailability of Heavy Metals in Biosolids‐Amended Soil

Abstract

A single biosolids application was made to 1.5×2.3 m confined plots of a Davidson clay loam (Rhodic Kandiudult) in 1984 at 0, 42, 84, 126, 168, and 210 Mg ha^?1. The highest biosolids application supplied 750 and 600 kg ha^?1 of Cu and Zn, respectively. Corn (Zea mays L.), from 1984 to 2000, and radish (Raphanus sativus L.) and romaine lettuce (Lactuca sativa var. longifolia), from 2001 to 2004, were grown at the site to assess heavy‐metal bioavailability. Extractable (0.005 diethylenetriamine (DTPA) and Mehlich 1) Cu and Zn were determined on 0 to 15‐cm depth samples from each plot. Corn yield increased with biosolids rate each year until 1993 to 1997, when yield decreased with biosolids rate because of phytotoxicity induced by low (<5.0) soil pH. The corn yield reduction was reversed between 1998 and 2000 upon raising the soil pH to approximately 6.0 by limestone addition following the 1997 season. Between 2001 and 2004, radish and lettuce yields were either not affected or slightly increased with biosolids rate, even as soil pH declined to below 5.5. Plant‐tissue metal concentrations increased with biosolids rate and as pH declined but were always within the normal range of these crops. Mehlich 1 and DTPA extractable metals increased linearly with biosolids rate. Extractability of Cu and Zn decreased approximately 50% over the past 20 years despite a decrease in soil organic matter concentration and greater than 95% conservation of the metals.     

Stretching Soil Sampling to Watershed: Evaluation of Soil‐Test Parameters in a Semi‐arid Tropical Watershed

Soil sampling is an integral component of fertility evaluation and nutrient recommendation for efficient use of nutrients in crop production. Little attention has been devoted to evaluating methodology for sampling watersheds under dryland agriculture. A stratified random sampling methodology for sampling the Appayapally watershed in Mahabubnagar district of Andhra Pradesh state in the semi‐arid tropical region of India was adopted and evaluated. The watershed has an area of about 500 ha, with gentle sloping lands (<1% slope), and 217 farmers own land in the watershed. The soils are Alfisols. A total of 114 soil samples were collected from the top 15‐cm layer to represent the entire watershed. Each sample was a composite of 7–8 cores, randomly collected from the area represented by a crop and group of farmers. The soil samples were air dried, ground, and analyzed for pH, electrical conductivity (EC), organic carbon (C), total nitrogen (N), and extractable phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na), sulfur (S), zinc (Zn), manganese (Mn), iron (Fe), copper (Cu), and boron (B). Statistical analysis of the results on soil fertility parameters showed that the mean‐ or median‐based results of soil tests performed in the study did not differ significantly when the sample set size varied from 5 to 114 (100% of the population). Our results indicate that farmers' fields in the Appayapally watershed are uniform in the chemical fertility parameters studied, and even a small sample set size can represent the whole population. However, such a sampling strategy may be applicable only to watersheds that are very gently sloping and where fertilizer use is very low, resulting in an overall low fertility in the whole watershed.     

Heavy‐Metal Contamination of Soil and Vegetables in Wastewater‐Irrigated Agricultural Soil in a Suburban Area of Hanoi,Vietnam

The To Lich and Kim Nguu Rivers, laden with untreated waste from industrial sources, serve as sources of water for irrigating vegetable farms. The purposes of this study were to identify the impact of wastewater irrigation on the level of heavy metals in the soils and vegetables and to predict their potential mobility and bioavailability. Soil samples were collected from different distances from the canal. The average concentrations of the heavy metals in the soil were in the order zinc (Zn; 204 mg kg^?1) > copper (Cu; 196 mg kg^?1) > chromium (Cr; 175 mg kg^?1) > lead (Pb; 131 mg kg^?1) > nickel (Ni; 60 mg kg^?1) > cadmium (Cd; 4 mg kg^?1). The concentrations of all heavy metals in the study site were much greater than the background level in that area and exceeded the permissible levels of the Vietnamese standards for Cd, Cu, and Pb. The concentrations of Zn, Ni, and Pb in the surface soil decreased with distance from the canal. The results of selective sequential extraction indicated that dominant fractions were oxide, organic, and residual for Ni, Pb, and Zn; organic and oxide for Cr; oxide for Cd; and organic for Cu. Leaching tests for water and acid indicated that the ratio of leached metal concentration to total metal concentration in the soil decreased in the order of Cd > Ni > Cr > Pb > Cu > Zn and in the order of Cd > Ni > Cr > Zn > Cu > Pb for the ethylenediaminetetraaceitc acid (EDTA) treatment. The EDTA treatment gave greater leachability than other treatments for most metal types. By leaching with water and acid, all heavy metals were fully released from the exchangeable fraction, and some heavy metals were fully released from carbonate and oxide fractions. The concentrations of Cd, Cr, Cu, Ni, Pb, and Zn in the vegetables exceeded the Vietnamese standards. The transfer coefficients for the metals were in the order of Zn > Ni > Cu > Cd = Cr > Pb.     

Boron partitioning in B‐sprayed and unsprayed fruiting twigs of hazelnut

Abstract

Following B sprays, fruit set increase in ‘Barcelona’ hazelnut was accompanied by a decrease in dry‐matter accumulation for the entire fruiting twig. In unsprayed controls prior to 5 June, leaves constituted 80% of the total dry weight of a fruiting twig. Later in the season (5 Aug.) the percent dry weight was maximum in the nut tissue (66%), followed by leaves (17%), and then husks (13%). Leaves contained 87% of the total B in a fruiting twig early in the season, indicating that the leaf was a major sink for B at that time. Of the net B increases over a 2 months period, 24% was in the leaves, 38% in the nuts, and 37% in the husks. By 5 Aug., compared to other tissues, stem dry matter (3%) and B content (32) was negligible; hence, the stem was of little importance in overall partitioning. Boron sprays did not alter B partitioning in the fruiting twigs.     

Soil‐grinder type effects on grams of soil scooped

Abstract

Soil preparation can affect soil test results. This study was conducted to compare soil‐grinder effects on grams of soil scooped. Soil samples (n=15,000) were ground by two types of soil grinders, a roller and hammer mill, and then the soil was sieved (10‐mesh), and scooped with a one‐gram scoop (0.85 cm³). The contents of the soil scoop were weighed. Grams of soil scooped ranged from 0.4 to 1.8 g for both soil grinders. The bell‐shaped distribution was centered around 0.8 and 1.0 g for the roller‐ and hammer mill‐type grinder, respectively. When the soil was ground by the roller‐type grinder, 85% of the ground soil was retained on a 50‐mesh sieve. In contrast, with the hammer mill grinder, 45% of the soil was retained on a 50‐mesh, 13% retained on a 100‐mesh, and 42% passed a 100‐mesh sieves. The “heavier” soil scoops with the hammer mill grinder could be explained by the fact that the finer soil particles could pack into the voids that the coarse soil particles created. Bray extractable P and ammonium actetate extractable K, Mg, and Ca were increased 11 to 15% by the “heavier” scooping weight. Grams of soil scooped and soil test levels were affected by the type of soil grinder and soil type.     

Evaluating Methods of In‐Field Soil Organic Matter Analysis

Abstract

The actual content of the soil organic carbon (SOC) has to be periodically measured for soil classification and nutrient management purposes. Traditional SOC tests are relatively time consuming and costly. A rapid field test would be valuable to delineate soil map units with similar SOC to simplify the process of land evaluation while increasing precision. The objectives of this study were to develop and evaluate a new field measurement technique for the quick assessment of SOC. The new method measures the emitted CO₂ concentration 3 min after treatment of the soil sample with acidic potassium (K) permanganate solution. The inorganic carbonate content of the soil is measured separately with the addition of sulphuric acid only. Carbon dioxide concentration from both procedures is measured with a portable infrared gas analyzer. The difference between the concentrations measured after the two separate reactions provide an estimate of SOC. Samples from brown forest soils (ca Hapludalf) (0.19–5.53% SOC) were used for the method development. The correlation coefficient between the SOC determined by the new method and laboratory wet combustion method content was 0.76 for the full range of SOC and 0.81 for the soil samples with less than 20% carbonate.     

Use of Chromameter‐Measured Color Parameters in Estimating Color‐Related Soil Variables

Abstract

Soil color is a soil property that may be used as an interpreting index in estimating processes and properties. Quantifying color allows one to obtain information for rapidly estimating the related processes in soils. CIELAB color parameters L*, a*, and b* of ground (air‐dried and sieved) soil samples and aggregate surfaces of four soil profiles formed in calcareous colluviums in northern Turkey were analyzed. Values of color parameters measured in ground and intact soils were compared and related to soil properties by the regression technique. Results revealed that the L* value obtained with a ground soil sample was a significant predictor of organic matter in A horizons (p < 0.001). Although calcic horizons yielded the highest L* values, no significant relationship was obtained between calcium carbonate contents and lightness of soils in any of horizons studied. The parameters of b* could adequately be used to quantify dithionite‐extractable iron oxide contents in soils studied. The results further showed that the CIELAB color parameters obtained with ground samples were more informative than that of aggrevated samples in relating color parameters to soil properties.     

Re‐analysis of the Relationship between Organic Carbon and Loss‐on‐Ignition in Soil

The suitability of loss‐on‐ignition (LOI) as an alternative to direct measurement of organic carbon (OC) has been debated for decades without resolution. The literature contains an abundance of different linear regression models to describe the LOI–OC relationship, most based on untransformed values of LOI and OC. Such regression is suspect because the variables are unable to occupy Euclidean space. Logratio transformation—based on relative rather than absolute differences—eliminates this constraint. Re‐analysis of the relationship on new and 10 previously published datasets using logratio techniques reveals that the relationship is nonlinear and that the profusion of regression models is in part a function of the range of LOI. Although LOI may offer a crude estimate of OC at high LOI levels, OC/LOI ratios when LOI is less than about 25% are too variable for reliable OC estimation, and interstudy comparisons remain dubious. Direct measurement of OC is recommended.     

Integration of Soil pH with Soil‐Test Values of Zinc for Prediction of Yield Response in Rice Grown in Mollisols

Seventeen Mollisols having pH_(1:2) in the range of 6.00 to 8.42 were analyzed with five extractants, and the extractable zinc (Zn) ranges were 0.84 to 2.75 mg Zn kg^?1 soil for diethylenetriaminepentaacetic acid (DTPA) (pH 7.3), 0.91 to 2.72 mg Zn kg^?1 soil for DTPA + ammonium bicarbonate (pH 7.6), 1.82 to 7.18 mg Zn kg^?1 soil for Mehlich 3, 1.22 to 3.83 mg Zn kg^?1 soil for ethylenediaminetetraacetic acid (EDTA) + ammonium carbonate, and 0.88 to 1.18 mg Zn kg^?1 soil for 1 mol L^?1 magnesium chloride (MgCl₂) (pH 6.0). Zinc extracted by DTPA (pH 7.3) and Mehlich 3 showed significant positive correlation with sand content, whereas only Mehlich 3 showed negative correlation with soil pH. All extractants showed significant positive correlation with each other except for 1 mol L^?1 MgCl₂‐extractable Zn, which had significant positive correlation with only Mehlich 3– and EDTA + ammonium carbonate–extractable Zn. A greenhouse experiment showed that Bray's percentage yield of rice was poorly correlated to extractable soil Zn but had a significant and negative linear correlation with soil pH (r = ?0.662, significant at p = 0.01). Total Zn uptake by rice had a significant positive correlation with 1 mol L^?1 MgCl₂– and Mehlich 3–extractable Zn. A proposed parameter (p extractable Zn + p OH^?) involving both soil extractable Zn and pH terms together showed significant and positive correlation with Bray's percentage yield and total Zn uptake of rice. The calculated values of critical limits of soil Zn in terms of the proposed parameter were 14.1699 for DTPA (pH 7.3), 13.9587 for DTPA + ammonium bicarbonate, 13.7016 for Mehlich 3, 13.9402 for EDTA + ammonium carbonate, and 14.1810 for 1 mol L^?1 MgCl₂ (pH 6.0). The critical limits of Zn in rice grain and straw were 17.32 and 22.95 mg Zn kg^?1 plant tissue, respectively.     

Extraction and reverse‐phase high‐pressure liquid chromatography of gibberellic acid in soil

Abstract

Several aqueous extractants buffered above pH 6.5 were evaluated for their ability to extract gibberellic acid from a Xerollic Calciorthid. More than 90% of the GA₃ added to soil was recovered with 0.01 M KH₂PO₄, pH 7.4. Gibberellic acid in soil extracts concentrated under vacuum was determined by reverse‐phase high‐pressure liquid chromatography (HPLC) by comparing the reproducible GA₃ peak areas with those of standards prepared in the same background solution.     

Evaluation of Soil Tests for Plant‐available Mercury in a Soil–Crop Rotation System

A reliable soil test is needed for estimating mercury (Hg) availability to crop plants. In this study, four extraction procedures including 0.1 M hydrochloric acid (HCl), 1 M ammonium acetate (NH₄OAc) (pH 7.0), 0.005 M diethylenetriaminepentaacetic acid (DTPA), and 0.1 M calcium chloride (CaCl₂) (pH5.0) were compared for their adequacy in predicting soil Hg availability to crop plants of a rice–cabbage–radish rotation system. The amounts of Hg extracted by each of the four procedures increased with increasing equilibrium time. The optimal time required for extraction of soil Hg was approximately 30 min, though it varied slightly among the four extractants. The amounts of Hg extracted decreased with increasing soil/solution ratio, and a soil/solution ratio of 1:5 appeared to be adequate for soil Hg availability tests. The amounts of Hg extracted increased in the order of NH₄OAc < CaCl₂ < DTPA < HCl in silty loam soil (SLS) soil, and the order was NH₄OAc < CaCl₂ ≈ DTPA < HCl in yellowish red soil (YRS) soil. Significant positive correlations among the four extractants were obtained in SLS soil. In contrast, the correlations were poor in YRS soil, especially for HCl. There were significant correlations between concentrations of Hg in edible tissue of three plants and the amounts of soil Hg extractable to the four extractants for soil–rice system and soil–radish system, but not for soil–Chinese cabbage system. The 0.1M HCl extraction overall provided the best estimation of soil‐available Hg and could be used to predict phytoavailability of Hg in soil–crop systems.     

Comparison of Kinetic Models for Boron Adsorption in Alluvium‐Derived Soils of Punjab,India

Abstract

The understanding of the boron (B) adsorption mechanism on soil materials is vital because plants respond primarily to the B activity in soil solution. Batch studies were conducted to investigate the adsorption behavior of B in 21 surface soils representing major soil series of Punjab. The soils varied widely in their properties. Six mathematical models (viz., zero order, first order, second order, Elovich, power function, and parabolic diffusion) were used to describe B adsorption. The B adsorption pattern was characterized by an initial fast reaction followed by a slow process, and it was complete in 24 h of equilibrium. The B equilibrium concentration was negatively correlated with clay content (r=?0.911**), organic carbon (OC) (?0.541*), and cation exchange capacity (CEC) (?0.540*) and positively with sand content (r=0.706**). The Elovich equation was best to describe the rate of B adsorption followed by the power function.     

Boron and ectomycorrhizal influences on mineral nutrition of container‐grown Pinus ehinata mill

Boron fertilization and inoculation with Pisolithus tinctorius (Pers.) Coker and Couch increased foliar and total seedling nutrient content of boron, calcium, copper, iron, potassium, magnesium, manganese and phosphorus in shortleaf pine seedlings (Pinus echinata Mill.). Noninoculated seedlings fertilized with boron showed no increase in nutrient content other than B. The increase in nutrient content of inoculated seedlings fertilized with boron was correlated with increased mycorrhizal infection. Boron fertilization may affect indirectly the mineral nutrition of tree seedlings by increasing mycorrhizal fungi colonization of their roots.     

Implementation of Soil Lime Requirement by a Single‐Addition Titration Method

Abstract

Buffers for determining a soil's lime requirement (LR) sometimes contain hazardous chemicals. Our objective was to implement a single‐addition titration with calcium hydroxide [Ca(OH)₂] to determine the LR of soils. The soil pH buffering capacity is calculated from the rise in pH from a single addition of base. The LR is calculated from the soil pH buffering capacity, the target pH, and initial soil pH. The LR of 531 randomly selected client samples determined by single‐addition titration were slightly higher than by the Adams–Evans (AE) buffer procedure when LRs were less than 1800 lb per acre. The new procedure recommended about 11% less lime than AE at LRs greater than 1800 lb per acre. Independent evaluations of samples that gave the most widely different LR revealed that the single‐addition titration was more accurate and more precise than the AE buffer.     

Boron fertilization,vesicular‐arbuscular mycorrhizal colonization and growth of citrus jambhiri lush

The role of boron (B) fertilization in the vesicular‐arbuscular mycorrhizal (VAM) colonization and growth of container‐grown Citrus seedlings was evaluated. Citrus jambhiri Lush, seedlings inoculated with Glomus fasciculatum, Glomus etunicatum or non‐inoculated were grown in a sandy loam soil for sixteen weeks. Seedlings were fertilized with 25 ug/ml B applied to the foliage as a mist, to the soil as a solution, or to both the foliage and the soil. Boron applied to the foliage or to the soil significantly increased root exudation of reducing sugars and amino acids two weeks after seedling germination. Subsequently, foliar fertilization with B significantly increased VAH colonization of seedlings relative to the controls. Plants inoculated with G. fasciculatum were larger and had greater VAM development than those inoculated with G. etunicatum. The growth of the noninoculated seedlings was not enhanced by B fertilization. The superior VAH colonization and growth of inoculated seedlings fertilized with B suggests that B stimulates the efficacy of plant fungi symbiosis.