闽南-台湾浅滩白姑鱼渔业生物学特性研究

利用2017年8月—2018年4月采集单船底层拖网调查的白姑鱼渔业生物学数据,研究了闽南-台湾浅滩渔场白姑鱼体长组成、体质量组成、体长与体质量关系、性比、性腺成熟度和摄食等级等渔业生物学特性。结果表明,白姑鱼体长范围为45～202 mm,优势体长组为120～150 mm,占总数48. 4%;体质量范围为2. 0～219. 0 g,优势体质量组为40. 0～70. 0 g,占总数43. 0%。各月份之间体长均值和分布均存在显著性差异(F=25. 161,P 0. 05);体长与体质量关系为W=2. 560×10-5L2. 971 7(R2=0. 983 8);雌雄性比为2. 16∶1,性腺成熟度以Ⅱ期为主,占86. 32%,未发现有Ⅴ期及以上样本;摄食等级以0～1级为主,占样本总数71. 11%,各月份摄食等级也存在显著性差异(F=10. 346,P 0. 05)。本次调查白姑鱼达到可捕体长的仅占16. 1%,说明拖网作业对幼鱼的损害比较严重。为持续利用白姑鱼资源,建议加强生殖群体和幼鱼群体的保护。     

西北太平洋秋刀鱼主要体征及繁殖特性研究

根据2014年6—7月和10—11月于西北太平洋公海两航次采集的秋刀鱼样本,分析其性别比例、体长和体质量组成、摄食等级,以探究其生长、性腺及繁殖特性。研究结果表明,随着月份的增大,秋刀鱼雌性比例逐渐降低,雄性占比升高;6、7月雌、雄样本优势体长组与体质量组范围均较10、11月小;体长和体质量关系中,秋刀鱼雌性生长系数为3.24,雄性为2.82;各月样本胃饱满度以1级为主,F检验表明,除0级外,4个月内各摄食等级间呈显著差异(P<0.01);秋刀鱼雌、雄样本性腺发育以Ⅱ期为主,各性腺等级间差异不显著(P>0.05),雌性性腺指数在Ⅱ、Ⅲ、Ⅳ期内大于雄性,Ⅴ、Ⅵ期内相反,逻辑斯蒂方程表明,秋刀鱼50%性成熟体长雌性为284.7 mm,雄性为267.4 mm;雌性秋刀鱼绝对繁殖力为1032～17 708粒/尾,平均值为(5522±3196)粒/尾,绝对繁殖力高的个体体长和体质量分别为280～320 mm、110～150 g。     

马尔代夫海域金枪鱼延绳钓渔场大眼金枪鱼生物学特性

根据2003年12月16日至2004年6月8日马尔代夫水域金枪鱼延绳钓渔获物生物学测定数据，应用统计与回归的方法分性别分别对296尾大眼金枪鱼（Thunnus obesus）的性腺成熟度、摄食等级、摄食种类、性别比例、叉长分布、叉长与去鳃去内脏的重量（GWT）的关系等进行研究。结果表明，（1）性腺成熟度以Ⅰ、Ⅱ和Ⅴ级为主，Ⅴ级比例最高，占37．50％，其中雄性大眼金枪鱼的性腺成熟度Ⅴ级最多（53．96％），雌性Ⅱ级最多（33．81％）。（2）摄食等级以0、1和2级为主，总计为88．52％。（3）大眼金枪鱼的摄食种类中，头足类（鱿鱼）或杂鱼的出现频率较高，分别占48．36％和24．59％。（4）雄性个体所占比例略高于雌性个体，雄性与雌性个体的性别比例为1．12：1，叉长1．00～1．20m的大眼金枪鱼雌性占52．08％，叉长1．20～1．40m的个体雌性占52．59％，叉长1．40m以上的个体，雌性占35．14％。（5）优势叉长为1．10～1．40m，平均叉长为1．29m。（5）根据幂函数回归所得叉长（FL）与去鳃去内脏的重量（GWT）关系式显示，在叉长小于1．51m时，雌性的去鳃去内脏重量小于雄性的去鳃去内脏重量；而在叉长大于1．51m时，雌性的去鳃去内脏的重量大于雄性的去鳃去内脏的重量。[中国水产科学，2006，13（4）：674—678，649]     

印度洋黄鳍金枪鱼生物学特性的初步研究

根据2003-2004年1-6月印度洋中西部海域金枪鱼延绳钓所捕获的黄鳍金枪鱼数据,对其基本的生物学特征进行了研究。结果表明,2003年和2004年渔获物中黄鳍金枪鱼加工后体重分别为7~81 kg和20~78kg,优势组分别为20~50 kg和30~60 kg。叉长范围分别为70~180 cm和110~170 cm。2003年渔获物雌雄性比的平均值为0.71,月间性比组成差异较小;2004年渔获雌雄性比的平均值为1.00,月间性比组成差异较大。2003年各月的摄食等级以1-2级为主,月间差异较小;2004年摄食等级以一级为主,并且月间各等级组成差异大。当渔获个体的叉长小于100 cm时,其性比组成波动较大;当渔获个体的叉长范围在100~150 cm之间时,性比组成的变化相对较小,基本上维持在0.5左右,也就是说雌雄个体数量大体保持平衡;当渔获个体的叉长大于160 cm时,所有样本个体均为雄性。体长与体重关系为W=2×10-5L2.9269。     

闽南、台湾浅滩渔场二长棘鲷群体结构特征

根据1998年7月至2000年6月闽南、台湾浅滩渔场二长棘鲷的生物学测定资料，本文主要研究其群体组成、生殖、摄食等特点，为合理开发利用和管理二长棘鲷资源提供科学依据。二长棘鲷周年叉长优势组为110—140mm，占58．9％，体重优势组为30—70g，占64．6％，平均叉长为123mm，平均体重为55．3g；由0-Ⅵ龄7个年龄组所组成，以0龄组占优势，平均年龄仅0．71龄，雌性性腺成熟度以Ⅱ期为主，其初次性成熟的叉长为117mm，生殖期为12月至翌年3月；周年均有摄食，各月摄食强度变化较大，平均摄食等级为1．46，平均摄食率为88．8％，摄食强度与叉长成显著的正相关。研究结果表明二长棘鲷种群结构趋于简单化、小型化、低龄化。     

印度洋中西部黄鳍金枪鱼生物学特性的初步研究

根据2003~2004年1~6月我国农业部印度洋金枪鱼延绳钓观察员项目所收集的有关数据,对印度洋中西部黄鳍金枪鱼的基本生物学特征进行了研究。结果表明,2003年和2004年渔获物中黄鳍金枪鱼加工后体重范围分别为7~81kg和20~78kg,优势组分别为20~50kg和30~60kg。叉长范围分别为70~180 cm和110~170 cm。2003年渔获物雌雄性比的平均值为0.71,月间性比组成差异较小;而2004年渔获雌雄性比的平均值为1.00,月间性比组成差异较大。2003年各月的摄食等级以1~2级为主,月间差异较小;而2004年摄食等级以1级为主,并且月间各等级组成差异大。体长与体重关系为W=2.1890×10-5L2.9269。     

西北太平洋公海日本鲭生物学特征研究

日本鲭(Scomber japonicus)是西北太平洋公海灯光围网渔业捕捞的主要经济品种，具有巨大的开发利用价值。为全面了解其生物学特征，根据2016—2021年在该海域的渔业生产调查资料，对日本鲭叉长和体质量组成、叉长-体质量关系、性比、性腺成熟度、初次性成熟叉长、摄食等级和肥满度进行了研究。结果显示，其叉长和体质量呈双峰分布，叉长范围为102～400 mm,体质量范围为9.6～898.8 g。叉长-体质量关系式为W=1.303 84×10^-6L^{3.381 56},呈正向异速生长，性别对叉长-体质量关系影响不显著(P>0.05)。雌雄比例为1.74∶1,与1∶1差异显著(P<0.05);性成熟度以Ⅱ期为主，叉长对性腺成熟度的影响极显著(P<0.01);雌性和雄性初次性成熟叉长分别为(298.08±4.32) mm和(287.82±4.26) mm。摄食强度以1～3级为主，叉长显著影响摄食强度(P<0.05),性别对其影响不显著(P>0.05);肥满度范围为0.64～2.45,随叉长增加而增大。研究结果丰富了西北...     

东太平洋南北部海域大眼金枪鱼生物学特性对比研究

明确大眼金枪鱼(Thunnus obesus)的生物学特征是进行种群资源评估并依此制定和实施养护管理措施的重要前提。为掌握广泛的时间和空间范围内东太平洋大眼金枪鱼种群特征, 本研究利用 2013—2019 年执行东太平洋金枪鱼延绳钓资源探捕调查期间采集的大眼金枪鱼渔获数据, 对东太平洋南、北部海域大眼金枪鱼的叉长、净重、摄食等级、性腺成熟期等生物学特性进行了分析, 结果显示: (1)北部海域大眼金枪鱼的叉长范围为 72~229 cm, 优势叉长为 80~160 cm, 平均叉长(121.53±29.11) cm, 雌雄比为 0.91∶1, 优势性腺成熟期为 III、Ⅳ期; (2)南部海域大眼金枪鱼的叉长范围为 63~209 cm, 平均叉长为(134.87±25.73) cm, 优势叉长组 110~150 cm, 雌雄性比为 0.62∶1, 优势性腺成熟期为 III、Ⅴ期; (3)南北海域大眼金枪鱼的优势摄食等级分别为 1 级和 2 级, 空胃率分别为 46.98%和 3.22%, 总体上摄食强度随叉长的增大而增加; (4) ANCOVA 结果显示, 南部和北部海域叉长与净重的关系存在较明显的差异(P<0.05); (5) ANOVA 结果显示, 大眼金枪鱼雌雄个体性腺成熟差异不显著(P>0.05); (6)钓获后处于存活状态的个体占比较高, 死亡状态仅占 8.52%。研究表明, 东太平洋南北部海域大眼金枪鱼的叉长、性比和摄食强度等生物学特性具有一定的空间异质性。     

马鞍列岛海域皮氏叫姑鱼渔业生物学初步研究

基于2009年1月—2010年2月渔业资源调查数据,研究了马鞍列岛海域皮氏叫姑鱼的体长组成、体长与体质量关系、性比、繁殖习性和摄食等渔业生物学。结果表明:1)马鞍列岛海域皮氏叫姑鱼群体体长范围30～264 mm,优势体长组为80～100和130～180 mm;2)皮氏叫姑鱼幼鱼和雄性个体的生长呈负异速生长的特性,而雌性个体呈正异速生长的特性;3)雌雄比为1.68∶1(χ2=35.636,P<0.001),雌雄比随月份和体长变化,除8月和9月及体长110～150和160～180 mm外,雌雄个体数差异明显;4)皮氏叫姑鱼的繁殖期从6月持续到9月,其雌性和雄性个体首次性成熟体长分别为124.8和136.4 mm;5)皮氏叫姑鱼的饵料生物随月份和个体生长发生转变,幼鱼主要摄食端足类,而成鱼在繁殖前期主要摄食鱼类和虾类,繁殖期间主要摄食端足类。与其他海域相比,马鞍列岛海域皮氏叫姑鱼群体具有较大的体长范围和优势体长组体长。为持续利用皮氏叫姑鱼资源,建议加强繁殖群体和幼鱼群体的保护。     

南海中北部尾明角灯鱼渔业生物学特性的初步研究

根据2014年和2015年在南海中北部海域中层拖网采集的尾明角灯鱼(Ceratoscopelus warmingii)样本,对其体长和体质量组成、性比、性腺成熟度和摄食等级等生物学特性进行了初步分析。结果显示,尾明角灯鱼的体长为32~76 mm,优势体长组为45~60 mm,占总数的86.9%,平均体长为(51.47±6.60)mm;体质量为0.3~4.3 g,优势体质量组为1.0~2.5 g,占总数的91.1%,平均体质量为(1.6±0.61)g;雌雄个体大小不存在显著差异(ANOVA,P0.05);生长参数b为2.581,表明尾明角灯鱼为异速生长;雌雄性比为1.39∶1,在不同月份、不同体长组中差异较大,10月和1月雌性比例远高于雄性;不同月份均有性成熟个体,性腺成熟度以Ⅰ期和Ⅱ期为主,性腺成熟比例最高为1月;初次性成熟体长为51.4 mm;摄食等级0~4级所占比例分别为10.8%、46%、33.9%、8.1%和1.3%。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Rare serotype occurrence and PFGE genotypic diversity of Streptococcus agalactiae isolated from tilapia in China

Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China.     

Evaluation of a Fast Method Based on the Presence of Two Restriction Sites in the Mitochondrial ND5 (mt ND5) Gene for the Identification of Scomber Species

The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Growth hormone (GH) and reproduction: a review

Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations.     

Purification and partial characterization of GtHs (cfLH and cfFSH) from Indian walking catfish (Clarias batrachus) (L.) and development of a homologous ELISA for cfLH

Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL^?1 (1.25 ng well^?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation.     

The optimal dietary protein level of juvenile silver sillago Sillago sihama at three dietary lipid levels

A 5 × 3 factorial growth trial was conducted to evaluate optimal dietary protein and lipid levels (dietary protein level, DP; dietary lipid level, DL) for juvenile Sillago sihama (S. sihama) (2.0 ± 0.02 g, initial weight). Fish were fed 15 diets containing 5 DPs (350, 400, 450, 500 and 550 g/kg) and 3 DLs (60, 90 and 120 g/kg) for 8 weeks. The interaction between proteins and lipids significantly influenced the feed conversion ratio, condition factor, body composition, antioxidant indices and lipase activity (p < .05). DP 450 g/kg showed the highest average final body weight. DPs 500 and 550 g/kg significantly decreased the protein efficiency ratio (p < .05). DL 120 g/kg showed the highest percentage weight gain. The low feed conversion ratio was found in diets P45L12, P55L9 and P55L12. Diet P45L12 showed high superoxide dismutase activities. DP 450 g/kg showed the lowest average malondialdehyde content. Lipase activity was increased by increasing DP (p < .05) with a fall at DP 550 g/kg. Under the present experimental conditions, the optimal DP for S. sihama was 450 g/kg under the DL 120 g/kg.