共查询到10条相似文献,搜索用时 718 毫秒
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烟草绿色防控技术是发展现代烟草农业的趋势和需要,对提高烟叶质量,保障烟叶质量安全具有重要意义。本试验于2020年在云南省楚雄烟区武定县猫街镇开展,对比300、450和600张/hm2三种不同挂板密度情况下蓝板防治蓟马效果,得出结论:蓝板在田间最佳的挂板密度为600张/hm2时,诱杀蓟马效果最好,适合云南省武定县烟田更好诱杀蓟马,降低番茄斑萎病发生。 相似文献
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Tomato mottle mosaic virus: Characterization,resistance gene effectiveness,and quintuplex RT-PCR detection system
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Carlos Kwesi TETTEY YAN Zhi-yong MA Hua-yu ZHAO Mei-sheng GENG Chao TIAN Yan-ping LI Xiang-dong 《农业科学学报》2022,21(9):2641-2651
Tomato mottle mosaic virus (ToMMV), an economically important species of the genus Tobamovirus, causes significant loss in yield and quality of tomato fruits. Here, we identified the Shandong isolate of ToMMV (ToMMV-SD) collected from symptomatic tomato fruits in Weifang, Shandong Province of China. ToMMV-SD caused symptoms such as severe mosaic, mottling, and necrosis of tomato leaves, yellow spot and necrotic lesions on tomato fruits. The obtained full genome of ToMMV-SD was 6 399 nucleotides (accession number MW373515) and had the highest identity of 99.5% with that of isolate SC13-051 from the United States of America at the genomic level. The infectious clone of ToMMV-SD was constructed and induced clear mosaic and necrotic symptoms onto Nicotiana benthamiana leaves. Several commercial tomato cultivars, harboring Tm-22 resistance gene, and pepper cultivars, containing L resistance gene, were susceptible to ToMMV-SD. Plants of Solanum melongena (eggplant) and Brassica pekinensis (napa cabbage) showed mottling symptoms, while N. tabacum cv. Zhongyan 100 displayed latent infection. ToMMV-SD did not infect plants of N. tabacum cv. Xanthi NN, Brassica rapa ssp. chinensis (bok choy), Raphanus sativus (radish), Vigna unguiculata cv. Yuanzhong 28-2 (cowpea), or Tm-22 transgenic N. benthamiana. A quintuplex RT-PCR system differentiated ToMMV from tomato mosaic virus, tomato brown rugose fruit virus, tobacco mosaic virus, and tomato spotted wilt virus, with the threshold amount of 0.02 pg. These results highlight the threat posed by ToMMV to tomato and pepper cultivation and offer an efficient detection system for the simultaneous detection of four tobamoviruses and tomato spotted wilt virus infecting tomato plants in the field. 相似文献
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Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection
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Zhi-yong YAN Mei-sheng ZHAO Hua-yu MA Ling-zhi LIU Guang-ling YANG Chao GENG Yanping TIAN Xiang-dong LI 《农业科学学报》2021,20(7):1871-1879
Tomato brown rugose fruit virus(ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry. So far, it has spread to 10 countries in America, Asia, and Europe. In 2019, ToBRFV was identified in Shandong Province(ToBRFV-SD), China. In this study, it was shown that ToBRFV-SD induced mild to severe mosaic and blistering on leaves, necrosis on sepals and pedicles, and deformation, yellow spots, and brown rugose necrotic lesions on fruits. ToBRFV-SD induced distinct symptoms on plants of tomato, Capsicum annumm, and Nicotiana benthamiana, and caused latent infection on plants of Solanum tuberosum, Solanum melongena, and N. tabacum cv. Zhongyan 102. All the 50 tomato cultivars tested were highly sensitive to ToBRFV-SD. The complete genomic sequence of ToBRFV-SD shared the highest nucleotide and amino acid identities with isolate IL from Israel. In the phylogenetic tree constructed with the complete genomic sequence, all the ToBRFV isolates were clustered together and formed a sister branch with tobacco mosaic virus(TMV). Furthermore, a quadruplex RT-PCR system was developed that could differentiate ToBRFV from other economically important viruses affecting tomatoes, such as TMV, tomato mosaic virus, and tomato spotted wilt virus. The findings of this study enhance our understanding of the biological and molecular characteristics of ToBRFV and provide an efficient and effective detection method for multiple infections, which is helpful in the management of ToBRFV. 相似文献
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1989~1992年共采集标本1087份,共鉴定真菌、细菌、线虫病害33种,与1984年云南省病虫草普查时所记载的烟草病害16种有所增加。由于这次标本采集包括了云南省玉溪、曲靖、红河、大理、昭通、红河、昆明等主要烟区,基本代表了云南省的情况。调查研究结果为今后制定病害防治决策、进行综合治理奠定了基础。 相似文献
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西花蓟马 (Frankliniella occidentalis) 属缨翅目(Thysanoptera)蓟马科 (Thripidae) 昆虫。原产于北美洲,目前已成为一种具有严重危害的世界性的大田和温室作物害虫。西花蓟马不仅能通过直接取食对寄主植物造成危害,还能传播多种 Tospoviruses 属的病毒,对寄主植物造成间接危害,对其的防治一直是世界各国植保工作者研究的重要课题。本文主要论述了当前寄主植物对西花蓟马抗性研究方面的最新进展。 相似文献
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利用无致病力青枯菌株防治番茄青枯病的研究 总被引:25,自引:1,他引:24
自发突变的无致病力番茄青枯菌株Tm3具有产生细菌素的能力,对番茄青枯菌株Tm46有较强的抑菌作用,且能诱导烟草植株产生过敏性反应。盆栽和小区试验结果表明,Tm3具有较好的防治番茄青枯病的能力。防病机理可能是细菌素的抗生素和诱导植物抗病性的双重作用。 相似文献