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1.
Masanobu Tabata Takashi Kato Masatoshi Ohkubo Yasuhisa Abe Shuichiro Yoshinaga 《Journal of Forest Research》2002,7(2):105-112
The rot ofChamaecyparis obtusa (hinoki) trees was studied in the northern part of Kagawa Prefecture, Japan. Among 158 hinoki examined, butt rot was found
in 28.6, 58.3, and 100% of trees in the 29, 30, and 34-year-old stands, respectively. All trees with butt rot, and 11 trees
without it had rotted roots. Thirteen hinoki were peeled byCervus nippon (sika deer) and all of them were infected with butt rot. The maximum height of rot in deer-damaged trees was 2.6 m from the
ground level, but was 1.9 m in undamaged trees. About eighty-two percent of rotted trees showed rotted areas of less than
40 cm2 on the cross section of stems at the ground level. White mycelia and black flecks sometimes appeared in the rotted wood.
Basidiocarps of polyporaceous fungus were often found on felled logs and rotted stumps of hinoki and identified asPerenniporia subacida. Basidiomycetous fungus was isolated frequently from rotted wood of roots and stems, and determined to beP. subacida by comparative study on cultural characteristics. An inoculation experiment and wood-decay test proved that the fungus was
the cause of the rot of hinoki. Few absorbing roots of living trees were found in the clayey subsurface soil of the high dry
bulk density and the less soil aeration. Some absorbing roots had root rot and the rot spread from the base of the absorbing
root to the central part of the woody root. This is the first report on the rot of hinoki caused byP. subacida in Japan. 相似文献
2.
Katsuaki Ishii 《Journal of Forest Research》2002,7(2):99-104
Hinoki cypress (Chamaecyparis obtusa) is one of the most important timber resource forest trees in Japan. Because seed production from a seed orchard of hinoki
cypress is not constant every year, micropropagation from a limited amount of material is useful. Up to now, the conventional
tissue culture method using solid medium has been used. Here a new method using liquid culture in tubes rotated vertically
is described. Shoot primordium of hinoki cypress was inoculated in Campbell and Durzan’s (CD) liquid medium containing different
cytokinins (6-benzylaminopurine (BAP), Zeatin, thidiazurone (TDZ)), and the container tubes were rotated vertically around
the axis at 2 times / min. Culture room temperature was 25°C and light condition was 16 h photoperiod per day of fluorescent
lamps. Zeatin at 1μM concentration was the best for maintaining the shoot primordium production and TDZ induced callus on the surface of the
shoot primordia. After shoot primordium multiplication in the liquid culture, they were transplanted to agar medium for shoot
elongation. A high concentration of agar (up to 16 g/L) or AVF (anti vitrification factor from Dr. Nairn, 1995) was effective
to prevent vitrification of the shoots. Transformation of shoot primordium was done using particle bombardment with vectors
containingβ-glucuronidase (GUS) gene or herbicide resistance gene (bar). Positive result for transient transformation was observed with the histo-chemical study for transformation with GUS. Integration
of a useful herbicidebar gene into the shoot primordium culture system was also tried and stably transformed plants were obtained. This is the first
report of stable transformation of Japanese conifer using practically useful gene.
The generous supply of AVF-B from Dr. B.J. Nairn, Tasman Forestry, NZ is also appreciated. 相似文献
3.
Variations in the compositions of low-boilingpoint (LBP) monoterpenes in needle samples of 50 hinoki (Chamaecyparis obtusa) trees were investigated using the headspace technique. Considerable compositional variations were revealed, especially in sabinene composition. The sabinene composition varied from 4.9% to 78.0% of the total LBP monoterpenes.-Pinene, myrcene, and limonene also showed considerable variations (9.0%–32.7%, 5.5%–22.6%, 3.6%–29.0% respectively). Analysis of the monoterpene composition allowed definition of four chemotypes based on the contingency table test. No correlation was observed between tree size and LBP monoterpene composition, indicating that the compositional variation in LBP monoterpene exists genetically in this population of hinoki.Part of this paper was presented at the 11th annual meeting of the Chugoku Shikoku branch of the Japan Wood Research Society, Matsue, September 1999 相似文献
4.
Variations in the composition of low boiling point (LBP) monoterpenes emitted from needle samples of 150 hinoki (Chamaecyparis obtuse) trees (30 strains, each with five clones) native to Shimane Prefecture, Japan, were investigated using a headspace technique.
The assays revealed considerable proportional variations especially in the amount of sabinene, which ranged from 24% to 78%
of the total LBP monoterpenes. The proportions of α-pinene, myrcene, and limonene negatively correlated with that of sabinene overall. In particular, the proportion of limonene
showed clear negative correlation with that of sabinene (r = −0.98). Differences in the proportion of sabinene among five
clones in each strain were less than 15% in 22 out of 30 strains, indicating that monoterpene composition is constitutively
steady in most strains. In a few strains, however, considerable variation in the composition was observed among clones. 相似文献
5.
Pollen dispersal was estimated in two test plots in a hinoki (Chamaecyparis obtusa) seed orchard using a chloroplast DNA marker, the spacer region between thetrnD andtrnY genes, and SSCP (single strand conformation polymorphism). In Plot 1, 2,020 seeds from 40 trees within 30 m of the marker
tree were analyzed using the PCR-SSCP method. In Plot 2, 1,850 seeds from 37 trees were analyzed in the same manner. The results
revealed that the maximum pollen dispersal distance in the two plots exceeded 25 m. Pollen dispersal appeared to be inversely
proportional to the distance from the marker tree. The effective pollen dispersal was suggested to be less than about 20 m
in a mature hinoki seed orchard. Adjacent trees had an excessive influence when the pollen density was increased by artificial
flower stimulation. Therefore, it was suggested that seed production better resembles ideal random mating when carried out
as naturally as possible. In conclusion, the SSCP chloroplast DNA marker was a useful tool for amassing basic information
on pollen management in seed orchards of coniferous species. 相似文献
6.
Genetic variation in seed/cone production among clones was studied in a hinoki (Chamaecyparis obtusa Endl.) seed orchard containing 25 plus-trees by analyzing the number of cones, the yield of cones and seeds of individual
ramets for 5 successive years (1982 to 1986). There was significant variation among clones each year and parental contribution
in the seed orchard. Specifically, in the years 1982, 1983, 1984, 1985, and 1986, 20% of the clones produced 37.2, 60.6, 36.0,
44.3, and 44.8% of the total cones, respectively. The size of the crop greatly influenced the parental balance in the resulting
seed/cone crops. The product moment correlation coefficients and Spearman’s coefficients of rank correlation were small and
insignificant between consecutive years, but large and highly significant between alternate years, suggesting the presence
of carry-over effects in seed/cone production. The broad-sense heritability on a clone mean basis was 0.74 ± 0.15 for the
number of cones, 0.72 ± 0.14 for the yield of cones, and 0.68 ± 0.13 for the yield of seeds. The corresponding heritabilities
from analyses combined over all years were 0.24, 0.558, and 0.724, respectively. These results indicate that seed/cone production
in hinoki is under strong genetic control. Several managerial measures are discussed that maintain the genetic diversity in
seedlots used for reforestation, by reducing the variation in seed/cone production among clones and producing seed crops with
equal contributions from all parents. 相似文献
7.
Naoyuki Matsui Tatsuro Ohira Yasushi Hiramatsu Yasunori Imai Yoshifumi Miyazaki 《Journal of Wood Science》2007,53(6):529-532
Volatiles inside tatami mats containing hinoki (Chamaecyparis obtusa) wood-wool as padding were analyzed. Volatiles were collected with solid-phase microextraction (SPME) fibers in a small chamber
prepared in tatami mats and assayed by gas chromatography. Most of the detected compounds are typically found in hinoki extractives.
Monoterpenes rapidly decreased at the beginning of the experiment, while the dispersion of sesquiterpenes that contain hydroxyl
groups was observed to last more than 1 year. These sesquiterpenes, T-cadinols, and α-cadinols may play a role in the suppression of house dust mites.
Part of this report was presented at the 55th Annual Meeting of the Japan Wood Research Society in Kyoto, March 2005 相似文献
8.
Tomoaki Takahashi Kazukiyo Yamamoto Yosuke Miyachi Yoshimichi Senda Masashi Tsuzuku 《Journal of Forest Research》2006,11(2):117-123
Penetration rates of laser pulses transmitted from a small-footprint airborne light detection and ranging (LiDAR) system in
closed-canopy, middle-aged (40–50 years old) hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.) and sugi (Cryptomeria japonica D. Don) plantations that had similar levels of canopy openness were studied. The number of transmitted pulses was 107 427
points/ha for the hinoki cypress stand and 122 883 points/ha for the sugi stand. The penetration rates of the first (Pf) and second pulses (Ps) that reached the ground were 0.1% and 1.0% in the hinoki cypress stand, and 2.6% and 5.5% in the sugi stand, respectively.
Penetration rates for the total number of pulses (Pf+s) that reached the ground within each stand were 1.1% and 8.1%, respectively, and values for Pf+s, Pf, and Ps for sugi and hinoki cypress stands were significantly different (P < 0.001). The results of this study indicated that the generation of accurate digital terrain models in dense hinoki cypress
stands with complex topographies using only the data from laser pulses with poor laser penetration rates is likely to be difficult. 相似文献
9.
《Scandinavian Journal of Forest Research》2012,27(4):290-291
Abstract In 2001 large areas of Pinus sylvestris L. stands in Sweden were attacked by Gremmeniella abietina (Lagerb.) Morelet. The resultant damage was assessed in five genetic field trials and one clonal seed orchard, with the aims of quantifying the genetic variation in tree responses to G. abietina, studying the genetic relationship between G. abietina susceptibility and growth traits, and examining the implications of the results for P. sylvestris breeding. A comparison of G. abietina damage between plus-tree progenies and seed stand check-lots in each of four tree classes (dominant, co-dominant, subordinated and suppressed) found no significant differences except in the suppressed tree class, where plus-tree progenies were damaged less severely than trees from the seed stands. Narrow- and broad-sense heritability for G. abietina damage traits were in the range 0.22–0.42. Genetic correlations between G. abietina damage and growth traits were weak and not significantly different from zero. The results imply that selection for growth capacity in P. sylvestris breeding programmes using plus-tree selection or field progeny tests does not increase susceptibility to G. abietina. In addition, there is scope for selecting genotypes with increased resistance if this is required in the future. 相似文献
10.
A novel compound, 8(17),12E,14-labdatrien-19-al (trans-communal), was isolated from ethyl acetate extract of young cones of hinoki (Chamaecyparis obtusa Endl.). The chemical structure of the compound was determined mainly with various nuclear magnetic resonance spectral techniques. Its stereochemistry was determined by derivation to a known compound,trans-communol. In addition to this compound, four known compounds — ferruginol, chamaecydin, 12-hydroxy-6,7-seco-abieta-8,l 1,13-triene-6,7-dial, and frans-communic acid — were isolated. All isolated compounds were subjected to an antifeedant bioassay against the pest insectSpodoptera litura. Results of the bioassay showed that chamaecydin and 12-hydroxy-6,7-seco-abieta-8,11,13-triene-6,7-dial had antifeedant activity.Part of this report was presented at 45th Annual Meeting of the Japan Wood Research Society, Tokyo, April 1995 相似文献
11.
An attempt was made to examine the possible connection between the various ratios of calcium/aluminum (Ca/Al) in the nutrient
solution of plant cultures and the active oxygen scavenging system of hinoki cypress (Chamaecyparis obtusa) seedlings. The hinoki cypress seedlings were transferred to nutrient solutions containing 5 mM AlCl3 together with various concentrations of Ca(NO3)2 in pots containing glass beads and Teflon tips. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase
(APX) and glutathione reductase (GR) in the needles were estimated at several stages during the 7-day treatment. The samples
treated with the lower Ca/Al solutions show the highest SOD activities. The activities of APX and GR, both of them involved
in the ascorbate-glutathione cycle, showed the same tendency with decrease to higher Ca/Al ratio. These results indicate that
rhizospheric Ca might compete with Al and ameliorate Al toxicity on and in the roots, the Al stress is not transformed to
the needles after a few days, and the ascorbate-glutathione cycle in the hinoki cypress needles might fluctuate and be suppressed
by the rhizospheric Al stress during the 7 days.
This work was supported in part by funding from the Japan Science & Technology Corporation, the CREST program 1996–2001, and
the Center for Forest Decline Studies. 相似文献
12.
The effect of temperature upon nighttime respiration was examined on four different sized sample trees in a 17-year-old hinoki
cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.) stand over two years. Seasonal changes inQ
10 values and their responses to mean temperature were investigated. On the basis of the monthly relationships between nighttime
respiration (r) and temperature inside a chamber (θ),r=r
0exp (kθ), theQ
10 value (=exp(10k)) was calculated. TheQ
10 values were high (Q
10≥3.0) in winter when mean air temperature was low, and gradually decreased toward summer (Q
10≤1.5) through spring with increasing temperature. TheQ
10 values were negatively correlated with mean air temperature. The response ofQ
10 values to mean air temperature was described by a single equation, regardless of tree size. This result, which might be characteristic
of this species, shows that respiration ofC. obtusa trees is promoted by slight increases of air temperature in winter season. On the other hand, temperature sensitivity of
total respiration reduced during growing season when ambient temperature was high. These chaning temperature sensitivity according
to seasons may depend on the seasonal change of the ratio of growth respiration to total respiration. It is concluded that
changes in temperature due to changing seasons not only change respiration rate, but also change the response of respiration
rate to temperature by shiftingQ
10 values. 相似文献
13.
We estimated the water status of six 31-year-old hinoki cypress (Chamaecyparis obtusa) trees that were struck by a large typhoon. Measurements were made of the trunk water-conduction area of xylem, leaf water potentials, and diurnal changes in trunk diameters. The secondary woodboring insects, Callidiellum rufipenne and Ovalisia vivata, on the trees were captured by the sticky-trap banding method. In trees on which these wood borers were captured, water conduction areas were reduced, leaf water potentials were lower, and trunk diameter changes (R) fluctuated during the day, suggesting that the trees had been damaged by the typhoon and were under water stress. The secondary woodboring insects attacked the water-stressed trees. 相似文献
14.
Tadashi Ogawa Chisato Matsumoto Chisato Takenaka Takafumi Tezuka 《Journal of Forest Research》2000,5(2):81-85
The effect of CA(NO3)2 on the active oxygen scavenging system in hinoki cypress (Chamaecyparis obtusa) seedlings cultured in a nutrient solution containing aluminum was examined. The hinoki cypress seedlings were transferred
to nutrient solutions containing 5 mM AlCl3 together with various concentrations of Ca(NO3)2 in pots containing glass beads and Teflon tips. The growth in height and dry matter allocation to each organ was little influenced
over a period of 12 weeks by either Al or the concentration of Ca(NO3)2. The activity of superoxide dismutase (SOD) in the needles was stimulated by Al, and the effect of Al was lowered significantly
by simultaneous application of 25 mM Ca(NO3)2. At week 1, the activity of catalase (CAT) in the needles was increased by Al, but the effect was no longer observed at week
12. The Al concentration in the roots was increased by treatment with Al, whereas the Al concentration in needles was not.
These results indicate that rhizospheric Al stress stimulates antioxidative enzyme activities in hinoki cypress needles and
the activation of the enzymes is suppressed by addition of Ca. The transmission of Al stress to the needles, which induced
a change in the enzyme activity, is not caused by the transfer of the Al ion itself from roots to needles.
This work was supported in part by funding from the Japan Science and Technology Corporation, the CREST program 1996–2001,
and the Center for Forest Decline Studies. 相似文献
15.
We evaluated the protective effects of floor cover against soil erosion in three types of forest located on steep slopes
under a humid climate: 22- and 34-year-old Chamaecyparis obtusa (hinoki), 34-year-old Cryptomeria japonica (sugi), and 62-year-old Pinus densiflora (red pine) stands. We measured sediment transport rates (sediment mass passing through one meter of contour width per millimeter
of rainfall), using sediment traps, before and after removing floor cover. Raindrop splash erosion was dominant in the experimental
stands. Floor cover percentage (FCP) during the preremoval stage varied from 50% to 100% among the four stands, and sediment
transport rates ranged from 0.0079 to 1.7 g m−1 mm−1. The rates increased to 1.5–5.6 g m−1 mm−1 immediately after removing floor cover, and remained high throughout the experiment. The presence of physical cover near
the ground has a crucial effect on sediment transport on forested slopes. The protective effect ratio (the ratio of the sediment
transport rate in a control plot to that in the removal plot) in a young hinoki stand, in which the FCP decreased markedly,
was 0.3 at most, which is close to the rate for bare ground. The protective effect ratio in the red pine stand was ≤0.003.
We concluded that the protective effect of floor cover in undisturbed forests in Japan differs by over two orders of magnitude,
based on comparisons with previous studies.
Received: March 11, 2002 / Accepted: August 16, 2002
Present address: Department of Forest Site Environment, Forestry and Forest Products Research Institute, Ibaraki 305-8687 Japan Tel. +81-298-73-3211;
Fax +81-298-74-3720 e-mail: miura@affrc.go.jp
Present address: Department of Forest Site Environment, Forestry and Forest Products Research Institute, Ibaraki 305-8687 Japan Tel. +81-298-73-3211;
Fax +81-298-74-3720 e-mail: miura@affrc.go.jp
Acknowledgments This study was supported by the Research Council of the Ministry of Agriculture, Forestry, and Fisheries, of Japan. We thank
H. Ujihara, S. Ujihara, and M. Ogasawara in Otoyo, Kochi, who provided the experimental stands used in this study. We also
thank K. Hirai, S. Kuramoto, E. Kodani, and the rest of the staff at the Shikoku Research Center, Forestry and Forest Products
Research Institute, for their help in conducting the experiments.
Correspondence to:S. Miura 相似文献
16.
The objective of this article is to investigate the variability in chemical composition, fiber length, and basic wood density (specific gravity) amongst clones. Wood samples were taken from an experimental plantation in a dense 2.5 × 2.5 m wood, i.e., 1600 trees/ha. The plantation consisted of 127 clones which were selected in the nursery from 11 eastern cottonwood (P. deltoides Bartr., section Aigeiros) half-sib progenies. The physical, structural, and chemical characteristics of 40 poplar clones aged four years were investigated in three model trees from each clone. There are significant differences between the contents of the individual components of the wood chemical composition. The values of the heritability factor are high and they are similar for lignin (0.936), holocellulose (0.937) and for the extractives content (0.999). The mean value of the basic wood density is 380 kg/m3 (coefficient of heritability is 0.938), and the mean value of the fiber length is 863 m (coefficient of heritability 0.611). 相似文献
17.
[目的]通过对思茅松自由授粉子代测试林的生长与形质性状遗传参数的估计,进行优良家系选择,为建立思茅松高世代种子园及培育优良无性系苗木提供种质资源。[方法]采用随机区组试验设计,在214个思茅松自由授粉家系子代测试林中选择胸径、地径、树高、枝下高、冠幅、通直度、树干圆满度、树冠圆满度、材积及地上生物量等生长与形质性状,利用线性混合模型进行遗传参数的估计,进行优良家系筛选。[结果]思茅松自由授粉子代测试林生长与形质性状的遗传参数在家系间差异极显著。各性状的家系遗传力较高,其中,地径的家系遗传力最大(1.105)。材积的预期遗传增益高于地上生物量,利用家系/家系内选择方法进行优良家系的筛选,材积的预期遗传增益为60.75%,入选家系为60个;地上生物量的预期遗传增益为44.22%,入选家系为66个。轮盘数、通直度和树干圆满度对材积和地上生物量都有显著影响。[结论]在早期选择中,形质性状的遗传变异对思茅松自由授粉优良家系的选择有重要参考作用,4年生思茅松自由授粉家系子代测试林中各性状存在着丰富的遗传变异,在早期选择中可以筛选出适合不同培育目标的优良家系,最大限度挖掘其遗传潜力。 相似文献
18.
Kei Kumakura Hiroyasu Kumakura Michiro Ogura Fumio Eguchi 《Journal of Wood Science》2008,54(6):502-508
We performed functional evaluation of the fruiting bodies of Ganoderma lucidum growing on ume trees (Japanese apricot, Prunus mume), and determined the suitability of pruned ume branches as a basic component of culture medium for this mushroom. We observed
that all tested functional activities of the fruiting bodies of G. lucidum collected from ume trees were higher than those collected from other broadleaf trees or cultured artificially; the functional
tests were angiotensin I-converting enzyme inhibitory activity, a platelet aggregation inhibition test, and an interleukin-8
(IL-8) gene expression inhibition test. When extracts from fruiting bodies of G. lucidum were orally administered to spontaneous hypertensive rats, hypotensive effects were found. Freeze drying was the most suitable
procedure for preservation of the extracts, and the activities of 30% ethanol extracts and 30% methanol extracts were higher
than those of hot-water extracts. The highest functional activities for extracts from G. lucidum mycelia cultured on sawdust media were for sawdusts based on ume wood.
Part of this study was presented at the 56th Annual Meeting of the Japan Wood Research Society, Akita, Japan, August 2006 相似文献
19.
A monoterpene and 15 diterpenes were isolated from the ethyl acetate extracts of the bark-glued resin from the resinous stem canker ofThujopsis dolabrata var.hondae Makino. A monoterpene (nezukone20) and 4 diterpenes (acetyl torulosol5, acetyl isocupressic acid8, acetyl abietinol11, and 7-methoxytotarol18) were characteristic constituents of the ethyl acetate extracts but were absent in then-hexane extracts from the resinous stem canker ofT. dolabrata var.hondae. These terpenes were first isolated fromT. dolabrata var.hondae andT. dolabrata. The available literature suggests that diterpene18 is a new compound. The resinous stem canker ofChamaecyparis obtusa Endlicher contained larger amounts of manool1,trans-communic acid6, and ferruginol12 and smaller amounts of isocupressic acid7 and abietinol10 than the resinous stem canker ofT. dolabrata var.hondae. The concentration of18 was less than 2% in the extracts, and the resinous stem canker ofC. obtusa lacked this compound. The resinous stem cankers ofC. obtusa andT. dolabrata var.hondae provided extracts 15.6 and 4.96 times, respectively, heavier than the healthy ones. Large differences in the ratios and compositions of terpenes were also observed between the resinous stem canker and the healthy trees. Terpenes isolated from the extracts contained many kinds of diterpene, especially the labdane-type diterpenes, in these diseased trees. These results suggest that the presence of labdane-type diterpenes is closely associated with the resinous stem canker or the causal fungi of this disease.Part of this report was presented at the 50th annual meeting of the Japan Wood Research Society, Kyoto, April 2000 相似文献
20.