天津部分猪场副猪嗜血杆菌病与猪传染性胸膜肺炎的流行病学调查

为了解天津部分猪场副猪嗜血杆菌病(HPs)和猪传染性胸膜肺炎(PCP)的流行情况,利用间接血凝试验对采自9个规模猪场的178份猪血清样品进行副猪嗜血杆菌抗体检测,对采自8个规模猪场的166份猪血清样品进行猪传染性胸膜肺炎放线杆菌抗体检测。结果表明:副猪嗜血杆菌阳性率为18.54%(33/178),其中断奶仔猪和保育阶段猪阳性率为23.58%(25/106);猪传染性胸膜肺炎的阳性率为21.08%(35/166),其中断奶仔猪阳性率为20.93%(9/43),育肥猪阳性率为27.66%(13/47),种猪阳性率为21.62%(8/37)。说明天津地区部分猪场的副猪嗜血杆菌病和猪传染性胸膜肺炎呈地方性流行。     

山西省部分猪场猪传染性胸膜肺炎血清学检测分析

[目的]掌握山西省猪传染性胸膜肺炎感染情况,为防控该病提供参考依据。[方法]用ApxIV-ELISA抗体检测试剂盒,对山西省11个市46个猪场851份猪血清进行猪胸膜肺炎放线杆菌ApxIV抗体血清学检测分析。[结果] 山西省猪胸膜肺炎放线杆菌抗体平均阳性率为57.11%;11个市猪场均有不同程度感染,感染率较高的是大同市、朔州市、忻州市,分别达到84.44%、83.75%和83.33%,临汾市感染率相对较低,为16.67%;母猪群体感染率最高,育肥猪次之,仔猪感染率最低;规模猪场感染率明显高于散养户感染。[结论] 猪传染性胸膜肺炎感染在山西省较为普遍,特别是母猪群阳性率达到74.60%,处在一个相对较高水平。     

赤峰地区猪传染性胸膜肺炎的血清学调查报告

对赤峰地区5个猪场送检的未免疫猪传染性胸膜肺炎疫苗的不同年龄阶段猪的338份血清样品,用间接血凝试验进行胸膜肺炎抗体检测。结果表明,所有猪场抗体均为阳性,平均阳性率63.15%;哺乳仔猪、育仔猪、生长育肥猪和成年种猪抗体阳性率分别达到3.23%、14.08%、91.14%、95.79%,总阳性率为52.07%。     

四川省部分地区猪传染性胸膜肺炎的流行病学调查

为弄清猪传染性胸膜肺炎在四川省的流行状况,笔者利用猪传染性胸膜肺炎抗体检测正向间接血凝试剂盒对在不同时间采集的、不同地区和不同来源的1062份非免疫猪血清进行了检测。结果不同地区猪群的猪传染性胸膜肺炎抗体阳性率不同,从1%-37%,平均阳性率为18.5%。其中,30日龄以内未断奶仔猪阳性率为1%、30-70日龄保育仔猪阳性率为23%、70-150日龄育肥猪阳性率为27%、后备母猪阳性率为8%、经产母猪阳性率为3%。以上结果表明猪传染性胸膜肺炎在四川省各地都有发生,且发生与猪的日龄、饲养方式有关。     

疫苗免疫防治猪传染性胸膜肺炎的作用分析

猪传染性胸膜肺炎是一种发病率较高的猪呼吸系统疾病,具有典型的胸膜肺炎和肺炎的病变特征与临床表现.猪传染性胸膜肺炎所导致的猪死亡率明显高于其他疾病,日龄在30~90之间的猪死亡率和发病率均高于20%,本次研究中,笔者对四个发生猪传染性胸膜肺炎的规模场进行了研究分析,对比试验猪传染性胸膜肺炎的灭活疫苗应用效果,将其分为90日龄以上育肥猪、30~90日龄仔猪、断奶前仔猪三组,并设置对照组,研究证实,注射灭活疫苗后发生猪传染性胸膜肺炎的仔猪在出栏前死亡率降低16.1%,发病率降低16.2%.     

河南省平顶山市猪传染性胸膜肺炎感染情况调查

为了弄清平顶山市猪传染性胸膜肺炎的感染情况与分布特征,于2016—2017年应用正向间接血凝实验(IHA)对平顶山市不同养殖类型的猪群进行猪传染性胸膜肺炎感染情况调查.结果显示:本次研究共调查368份猪血清样品,63份检测为阳性,阳性率为17.12%,其中不同地区猪群阳性率在11.96%~20.35%之间.不同发育阶段猪群阳性率有所差异,其中以育肥猪最高,为30.12%;哺乳仔猪最低,为9.59%.     

海南省规模猪场猪传染性胸膜肺炎的血清学调查

为了解海南省规模猪场猪传染性胸膜肺炎(PCP)的感染情况,采集海南省8个市县16个不同规模猪场血清样品1 286份,用ELISA方法对猪胸膜肺炎放线杆菌(APP)ApxⅣ抗体进行检测,分析APP的感染情况。结果显示,1 286份猪血清样品共检出阳性样品436份,抗体阳性率为33.90%。其中,检测母猪血清326份,抗体阳性率为47.85%;检测哺乳仔猪血清324份,抗体阳性率为37.65%;检测保育猪血清316份,抗体阳性率为18.99%;检测育肥猪血清320份,抗体阳性率为30.63%。8个市县均有APP感染,其中文昌市APP抗体阳性率最高;不同规模猪场均有APP感染,其中中型规模猪场APP抗体阳性率最高;不同品种猪均有APP感染,其中杜长陆三元杂猪APP抗体阳性率最高。说明PCP在海南省部分规模猪场中普遍存在,对猪场进行PCP的控制是十分必要的。     

上海市及周边部分地区猪传染性胸膜肺炎血清学调查与分析

为初步了解我国上海市及周边部分地区猪传染性胸膜肺炎放线杆菌(Actinobacillus pleuropneumoiae,APP)的感染情况,对2015~2016年间采集于上海市、江苏省、江西省以及浙江省部分地区的1031份猪血清进行胸膜肺炎放线杆菌感染情况检测。结果显示,上海市及周边地区部分规模化猪场猪群中APP抗体总阳性率为27.06%,其中APP抗体阳性率江西省(38.08%)、江苏省(33.09%)和浙江省(25.21%)均高于上海市(11.20%);种猪(37.74%)高于哺乳仔猪(33.16%)和育肥猪(25.96%)以及保育猪(14.68%);APP抗体水平夏季(40.25%)高于冬季(25.00%)、春季(28.21%)和秋季(15.29%);小型猪场(42.11%)高于中型(24.86%)和大型猪场(17.97%)中APP抗体阳性率。统计分析显示,APP抗体阴、阳性与地区、猪群的生长阶段、生长季节以及猪场规模有关(P0.001)。检测结果表明,我国上海市及周边地区猪群中胸膜肺炎放线杆菌感染较为普遍。本研究结果为我国猪传染性胸膜肺炎(porcine contagious pleuropneumonia,PCP)的防控以及净化工作提供了一定的数据支持。     

一起猪患传染性胸膜肺炎的防治

猪传染性胸膜肺炎又称猪副溶血杆菌病，是由猪胸膜肺炎放线杆菌引起一种以肺炎和胸膜炎为特征的呼吸道传染病。主要侵害仔猪和5月龄以前的育肥猪。我市某猪场，全场瘦肉型猪存栏1581头，其中种公猪9头、母猪270头、仔猪315头、育肥猪987头。于2004年4月15～22日，该猪场仔猪及育肥猪发病105头，     

猪传染性胸膜肺炎的血清学调查

为了解江苏省淮安市生猪传染性胸膜肺炎感染情况，应用ELISA试验对本市15个规模猪场共计300份血清样品进行猪传染性胸膜肺炎抗体监测。结果显示，被检样品中猪传染性胸膜肺炎抗体阳性率为0～30Z。平均为14．33Z，表明本地规模猪场有猪传染性胸膜肺炎感染，情况不容乐观。     

湖南省部分地区猪传染性胸膜肺炎的流行病学调查

为了解猪传染性胸膜肺炎在湖南省的流行状况,采用正向间接血凝试剂盒对在不同时间、不同地区和不同猪场采集的的267份非免疫猪血清进行了猪传染性胸膜肺炎抗体检测.结果发现,上述被检样品的总阳性率为34.46％,其中母猪阳性率最高为61.67％,30日龄猪阳性率为48.57％、60日龄猪阳性率为7.76％、90日龄猪阳性率为2...     

Prevalence of respiratory diseases and their association with growth rate and space in randomly selected swine herds.

The prevalence and extent of respiratory tract lesions were measured in 1425 pigs from 27 randomly selected herds in the summer of 1982 and winter of 1983. About 75% of pigs had lesions of enzootic pneumonia, approximately 60% had atrophic rhinitis and approximately 11% had pleuropneumonia and/or pleuritis. Individual pig growth rate was recorded on two of the farms, and it was found that the correlations between growth rate and severity of enzootic pneumonia lesions were positive on one farm and negative on the other. Negative correlations between severity of turbinate atrophy and growth rate existed in one of the two herds. Extent of pneumonia and severity of turbinate atrophy were poorly related in individual pigs but herd averages were moderately and positively correlated. Prevalence of diffuse pleuritis and of pleuropneumonia were positively related, as were the extent of pneumonia and prevalence of localized pleuritis. Prevalence of pleuropneumonia was strongly correlated with increased days-to-market. A method of estimating the average days-to-market using weekly herd data (inventory) was developed.     

湖北西南地区猪传染性胸膜肺炎的血清学调查

采用猪传染性胸膜肺炎间接血凝试验(IHA)对恩施州34家具有一定规模猪场的731份血清样品进行血清学调查,结果阳性率高达28.18%.其中被检仔猪血清206份,阳性42份,阳性率为20.39%;被检育肥猪血清237份,阳性67份,阳性率为28.27%;被检种猪血清288份,阳性97份,阳性率为33.68%.     

青海省大通地区猪传染性胸膜肺炎的血清学诊断

采用猪传染性胸膜肺炎间接血凝试验（IHA）对来自青海省大通地区的两个猪场的139份血清进行了猪传染性胸膜肺炎血清抗体检查,结果检出阳性90份,感染率为阳性率为64．75％。     

猪传染性胸膜肺炎PCR诊断方法的建立与应用

为调查猪传染性胸膜肺炎的流行状况,本试验建立了检测胸膜肺炎放线杆菌（Actinobacillus pleuropneaumoniae, App）的PCR方法。对186份病死猪病变组织及545份无临床症状健康猪鼻拭子进行了App的PCR检测。结果186份病料中App阳性率占43.0%（80/186）,545份猪鼻拭子中App阳性占9.4%（51/545）。该PCR方法可作为App临床诊断的重要手段之一。通过调查表明,聊城地区存在猪传染性胸膜肺炎放线杆菌的隐性感染及流行。     

An abattoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. III. Serological findings and their relationship to pathomorphological and microbiological findings

Blood samples from 777 pigs, originating from 9 different herds, were collected at slaughter and examined for antibodies to Mycoplasma hyopneumoniae and Actinobacillus (Haemophilus) pleuropneumoniae by the indirect hemagglutination assay (IHA) and the complement fixation (CF) test, respectively. Results were compared to pathological and microbiological findings. Antibodies to M. hyopneumoniae in positive titers of 1/80 or higher were found in 62% of the samples. The relationship between positive IHA titers to M. hyopneumoniae and gross findings indicative of enzootic pneumonia of pigs (EPP), histological findings indicative of EPP, the isolation of M. hyopneumoniae and the demonstration of M. hyopneumoniae by indirect immunofluorescent testing ranged from 64% to 68%. No correlation was noted between positive IHA titers and the isolation of Mycoplasma flocculare. Positive antibody titers to A. pleuropneumoniae of 1/10 or higher were detected in 5% to 85% of the samples from individual herds. Positive titers to A. pleuropneumoniae serotype 2 were found in 71% to 79% of the sampled animals from herds with high frequencies of pneumonic lesions indicative of pleuropneumonia. In herds with low frequencies of pleuropneumonia, positive titers were recorded in from 0 to 4% of the tested pigs. However, no statistical association was found between pleuropneumonia and positive titers to A. pleuropneumoniae serotype 2 in individual animals. Twenty-one per cent of samples with positive CF titers to A. pleuropneumoniae showed antibodies to more than one serotype.     

青海互助猪传染性胸膜肺炎的血清学检测

用猪传染性胸膜肺炎间接血凝试验（IHA）,对采自青海省互助县某猪场饲养的70份互助猪的血清,进行了猪传染性胸膜肺炎血清抗体的检测。结果：检出29份阳性血清。阳性率为41．43％,表明：青海省互助猪的猪群中存在猪传染性胸膜肺炎的流行。     

Serodiagnosis of pleuropneumonia using enzyme-linked immunosorbent assay with capsular polysaccharide antigens of Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7.

Capsular polysaccharide antigens of serotypes 1, 2, 5 and 7 of Actinobacillus pleuropneumoniae were used in enzyme-linked immunosorbent assays (ELISAs) to test sera from experimentally infected and field pigs. Specific reactions were found in sera of experimental pigs with antigens of serotypes 1, 5 and 7 whereas the serotype 2 antigen was cross-reactive. A 1:200 serum dilution was used for testing of 300 sera from 21 swine herds in southern Ontario. Cases of pleuropneumonia had occurred in 11 of these herds, but not in the others. The negative cut-off value was the mean optical density at 405 nm (OD405) + three standard deviations (SD) for 16 negative reference sera. Sera from four pigs naturally infected with Actinobacillus suis were tested and found to react to varying degrees with each of the antigens. Therefore a second cut-off value was determined as the mean OD405 + 2 SD for the A. suis sera. Sera which, in the ELISA produced OD readings above the latter cut-off were considered positive for antibodies to A. pleuropneumoniae; those which were lower than the former cut-off were considered negative. Readings between the two cut-off values may have been due to low positive titers or cross-reactivity, possibly with A. suis, and could not be used to predict pleuropneumonia. Of the pleuropneumonia-free herds, none had positive reactors to serotypes 5 or 7, whereas one and two herds had positive reactors to serotypes 1 and 2, respectively. Of the pleuropneumonia positive herds, six had positive reactors to serotype 1, one to serotype 2, four to serotype 5, and eight to serotype 7.     

Blood gas stability and hematological changes in experimentally-induced acute porcine pleuropneumonia.

Blood gas and hematological responses to acute, mild Actinobacillus pleuropneumoniae infection of growing pigs was studied. Six pigs (average weight 10.1 kg) were experimentally infected intranasally with A. pleuropneumoniae serotype 5. Four pigs served as controls. Rectal temperatures and arterial blood for gas analysis and hematology were taken at 0, 8, 16, 24, 48 and 72 h postinfection. All infected pigs became febrile showing clinical signs typical of mild to moderate porcine pleuropneumonia; controls remained asymptomatic. Neutrophilia with bands and lymphopenia were observed only in infected pigs. Arterial partial pressures of O2 and CO2, and pH did not change in infected pigs. All pigs were killed after 72 h, and lungs were examined and cultured. Gross and microscopic lesions consistent with porcine pleuropneumonia were seen in 3/6 and 5/6 infected lungs, respectively. Control lungs were grossly normal with no histological evidence of pleuropneumonia. We conclude that in mild, acute porcine pleuropneumonia as established experimentally, a leukogram typical of acute inflammation and stress is seen; however, hypoxemia and alveolar hypoventilation are not features of this form of the disease.