共查询到19条相似文献,搜索用时 609 毫秒
1.
美味侧耳子实体组织的细胞活力 总被引:2,自引:0,他引:2
依据子实体组织中的细胞活力与TTC-脱氢酶活性的正相关性,对生长发育的美味侧耳(Pleurotus sapidus)子实体各结构组织进行了TTC-脱氢酶的活性测定。结果是:随着侧耳子实体的生长发育菌龄 的增加,菌褶组织中的细胞活力逐渐增强,其它各结构组织中的细胞活力逐渐下降。菌柄和菌柄与菌盖之间的组织细胞活力以幼龄时期的最强,周边和表皮组织中的细胞活力,除幼龄时期的菌柄组织外,均高于同发育时期的其内部各结构组织中的细胞活力。 相似文献
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紫孢侧耳子实体组织的TTC——脱氢酶活性 总被引:1,自引:1,他引:0
本文报道紫孢侧耳(Pleurotus sapidus)子实体发育过程中,菌褶组织中的TTC-脱氢酶活性随着成熟度的增加逐渐增强,其它各结构组织中的TTC-脱氢酶活性逐渐降低。在子实体发育的幼小阶段。菌柄、菌柄与菌盖之间组织的脱氢酶活性高于菌褶和菌肉组织中的酶活。子实体的周边和表皮组织(菌盖直径在5cm以上)中的脱氢酶活性均高于其内部各结构组织中的酶活。 相似文献
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亮菌多糖ATPS-2对小鼠四氯化碳和酒精肝损伤的保护作用 总被引:2,自引:0,他引:2
分别采用四氯化碳(CCl4)和北京红星二锅头诱导小鼠肝损伤,比色法测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的含量;肝脏中超氧化物歧化酶(SOD)活力和丙二醛(MDA)浓度,并作肝组织切片病理观察。亮菌多糖ATPS-2(25mg/kg、50mg/kg、100mg/kg)给药明显降低小鼠血清中升高的ALT和AST水平,抑制肝脏中上升的MDA水平和提高过低的SOD活性。病理检查结果显示亮菌多糖ATPS-2有明显的保肝作用。亮菌多糖ATPS-2对小鼠四氯化碳肝损伤和酒精肝损伤均具有显著的保护作用。 相似文献
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对食用菌呼吸作用的研究,在国外也仅从食用菌与外界环境的关系来探讨,如对吸收的氧气、呼出的二氧化碳数量进行定量测定。使用这种方法要有较复杂的仪器和很高的实验条件。本文是从食用菌本身的生理生化变化入手,采用简便、准确的TTC 法,测定食用菌菌丝在子实体分化期脱氢酶活性的变化,来探讨其呼吸作用的变化规律及与食用菌品种特性的关系。 相似文献
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蜜环菌培养及浸膏提取工艺的初步研究 总被引:3,自引:0,他引:3
本文对蜜环菌培养需要的营养、温度、水份等要求进行了研究,采取三级培养固体发酵提取浸膏,特征反应明显,生产工艺简便,产品的各种理化指标符合规定要求。 相似文献
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Mahmood R Golzarian Ross A Frick Karthika Rajendran Bettina Berger Stuart Roy Mark Tester Desmond S Lun 《Plant methods》2011,7(1):2
With the establishment of advanced technology facilities for high throughput plant phenotyping, the problem of estimating
plant biomass of individual plants from their two dimensional images is becoming increasingly important. The approach predominantly
cited in literature is to estimate the biomass of a plant as a linear function of the projected shoot area of plants in the
images. However, the estimation error from this model, which is solely a function of projected shoot area, is large, prohibiting
accurate estimation of the biomass of plants, particularly for the salt-stressed plants. In this paper, we propose a method
based on plant specific weight for improving the accuracy of the linear model and reducing the estimation bias (the difference
between actual shoot dry weight and the value of the shoot dry weight estimated with a predictive model). For the proposed
method in this study, we modeled the plant shoot dry weight as a function of plant area and plant age. The data used for developing
our model and comparing the results with the linear model were collected from a completely randomized block design experiment.
A total of 320 plants from two bread wheat varieties were grown in a supported hydroponics system in a greenhouse. The plants
were exposed to two levels of hydroponic salt treatments (NaCl at 0 and 100 mM) for 6 weeks. Five harvests were carried out.
Each time 64 randomly selected plants were imaged and then harvested to measure the shoot fresh weight and shoot dry weight.
The results of statistical analysis showed that with our proposed method, most of the observed variance can be explained,
and moreover only a small difference between actual and estimated shoot dry weight was obtained. The low estimation bias indicates
that our proposed method can be used to estimate biomass of individual plants regardless of what variety the plant is and
what salt treatment has been applied. We validated this model on an independent set of barley data. The technique presented
in this paper may extend to other plants and types of stresses. 相似文献
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采用麦芽汁液体培养基培养蜜环菌获得菌丝体,采用麦芽汁半固体培养基培养蜜环菌获得蜜环菌菌素、皮壳状菌丝和子实体。以高效液相色谱-二极管阵列检测法(High Performance Liquid Chromatography-Diode Array detector,HPLC—DAD)比较四种菌体的甲醇提取物的成分差异。液体培养的蜜环菌菌丝体与固体培养的蜜环菌菌素、皮壳状菌丝和子实体的蜜环菌素类成分有明显差别。图谱分析表明,菌素与皮壳状菌丝的成分接近,与子实体和液体培养菌丝体之间有较大差别。 相似文献
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以邻苯二甲酸作为番茄的连作障碍自毒物质,珍珠岩为基质进行盆栽试验,并就生物量、光合作用和保护酶活性等指标的变化研究不同浓度邻苯二甲酸对番茄幼苗生长抑制作用以及加入碳化玉米芯缓解邻苯二甲酸对番茄幼苗生长抑制作用的效果。结果表明:施用邻苯二甲酸对幼苗的光合作用指标、生物量及叶绿素b的含量具有显著的抑制作用。保护酶活性在处理初期提高,随着处理时间的延长,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性先上升后下降,同时使幼苗体内MDA含量显著增加。加入碳化玉米芯显著提高了幼苗的生物量、光合作用指标及叶绿素b的含量,有效缓解了对保护酶活性的抑制作用,降低了保护酶活性变化的幅度及幼苗体内MDA含量。 相似文献
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朱缨花属(Calliandra Benth.)植物全球约125种,其中细叶粉扑花(Calliandra brevipes)、红粉扑花(Calliandra emarginata)和朱缨花(Calliandra haematocephala)等较为常见。通过对3种朱缨花属植物扦插幼苗的形态指标和生物量进行测量,采用多重比较和相关分析,研究同属植物间的差别和共性。结果表明:3种朱缨花属植物地下部分根的生长较为相近,根冠比差异不显著,但不同植物生物量的大小存在较大差异;根长、高径比(株高与地径的比值)与生物量不存在显著的相关性;地上、地下与总生物量之间相关性显著,其中又以地上部分生物量与总生物量的相关性较强。 相似文献
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梨园套种平菇对土壤生物活性及果实品质的影响 总被引:2,自引:0,他引:2
【目的】为探明套种平菇对梨园土壤生物活性及梨果实品质的影响,【方法】在7 a生梨树行间覆土套种平菇,将出菇后的菇渣旋耕还田,以不套种平菇为对照,测定分析梨园0~60 cm不同土层中细菌、放线菌、真菌数量,微生物量碳、量氮,脲酶、蔗糖酶、磷酸酶、过氧化氢酶活性以及成熟梨果实品质。【结果】结果表明,套种平菇显著提高0~40 cm土层3大类群土壤微生物数量、生物量及脲酶、蔗糖酶、磷酸酶、过氧化氢酶活性,并随套种年限的增加趋于增加。连续3 a套种平菇处理的细菌、放线菌、真菌数量较对照提高47.4%、32.0%、125.1%;微生物量碳、量氮比对照提高25.6%、28.1%;土壤脲酶、蔗糖酶、磷酸酶、过氧化氢酶活性提高42.3%、47.2%、29.5%、11.6%。土壤微生物数量、生物量、脲酶、蔗糖酶和磷酸酶活性随土层加深均有所降低,而过氧化氢酶活性则呈升高趋势。套种平菇后能不同程度改善果实品质,显著提高了梨果单果质量、硬度、可溶性固形物及可溶性糖含量,降低了可滴定酸含量。【结论】套种平菇对提高梨园0~40 cm土层土壤肥力、改善梨品质有显著效果。 相似文献
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One of the limiting factors to estimate accurately the biomass stocks of urban forests is the availability of allometric models developed from urban trees measurements. In addition, the traditional methods (destructive) to develop biomass equations are rarely applied in cities. The aim of this study was to develop a non-destructive method based on fractal analysis for trees of Fraxinus uhdei present in the green areas of Chapultepec Park in Mexico City. Diameter at breast height (DBH), total height and the height of the whorls of 46 randomly-selected trees were measured. In each tree, three different-order branches were collected to measure their total length and that of their links, the diameter before and after each branching point, the insertion angle, the number of branches, twigs and leaves, and fresh and dry weight. The felling of seven individuals at the site was taken advantage of to fit a taper function for the trunk and determine its biomass. Acceptable estimates with good accuracy were obtained for F. uhdei trees with a DBH of less than 23 cm. It is feasible to develop biomass models from measuring the branches and the trunk of young F. uhdei trees through the implementation of structural indices based on fractal geometry and without the need to fell healthy trees. 相似文献
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假植大苗定植对脐橙树体生长和产量的影响 总被引:1,自引:0,他引:1
在我国传统的柑橘建园中,种植裸根苗比较普遍,然而,这种种植方式的成活率较低,树体生长缓慢,投产较晚。多年来,在江西赣南脐橙产区建园中,大力推广假植大苗带土定植,即将脐橙苗假植于装有营养土的袋或篓(直径25cm×高30cm),集中管理并提供合适的环境条件,于第2年春梢老熟后(6月份)定植。这一改进,显著提高了成活率。对45个使用该方法的纽荷尔脐橙(Citrussinensiscv.Newhall)园共900株树进行了调查,同时,以传统的裸根苗直接定植作为对照。结果表明,假植大苗定植的树体生长各项指标(干周、树高、冠径和末级梢数量、质量)均明显优于对照;1-5年生幼树各项指标平均值相对于同龄对照有明显增加;4年生树干周、树高、冠径和末级梢数量比对照分别增加42.1%、42.6%、34.1%和31.7%。假植大苗定植第4年、第5年平均株产分别为19.4kg和42.5kg,显著高于同龄对照的5.1kg和27.5kg。假植大苗定植的植株树势一致,树冠形成快,投产早,经济效益显著,值得在生产上推广应用。 相似文献