Protein quality and digestibility of new high-lysine barley varieties in growing rats

Four new high-lysine barley mutants, the variety Lysimax, with the high-lysine genelys3a and the mutants mother variety Sultan were grown in a field trial in 1993 at Risø, Denmark. Mutants 609, 1242, 1385 and 1405 yielded in the range of 89 to 98 percent and cv Lysimax yielded 102 percent of cv Sultan (100 percent). One-thousand kernel weights for the mutants were in the range of 87 to 97 percent and cv Lysimax 83 percent of cv Sultan (100 percent). Protein contents of the mutants were slightly higher, in the range of 13.2 to 13.6 percent, than of cv Sultan (13.1 percent) and Lysimax which had a protein content of 12.6 percent. Fat content was higher in Lysimax and in the mutants except for mutant 1385 than in cv Sultan while dietary fibre contents of the barleys were similar. The levels of -glucans and starch were usually lower in Lysimax and in the mutants. The highest lysine levels: 4.6, 4.0 and 3.7 g/16 g N occurred in cv Lysimax and mutants 609 and 1405 compared to 3.3, 3.3 and 3.2 for cv Sultan and mutants 1242 and 1385, respectively. Mutants 609 and 1405 and cv Lysimax also had higher levels of threonine, histidine and valine. The increased lysine contents resulted in large, at most 20 percent, increases in biological value; 88.8, 81.7 and 78.3 percent for cv Lysimax and mutants 609 and 1405 compared to 74.2 percent for cv Sultan. True protein digestibilities and energy digestibilities were slightly lower in Lysimax than in Sultan, 5.3 and 4.3 percentage units, respectively. It is concluded that the development of high-lysine barley varieties is very beneficial for meeting the requirements of indispensable amino acids for humans and monogastric animals. In addition, nitrogen excretion into the environment is drastically reduced due to the higher biological values of the mutants.     

Microbiological evaluation of tofu and tempeh during processing and storage

Tofu samples consisting of Fresh Tofu, Herb Tofu, Tofu Paste, Fried Tofu, Tofu Burger, Smoked Tofu and different types of herbs used for making Herb Tofu were analysed for their microbiological load. Tempeh samples consisted of cook water, inoculated beans and fresh tempeh. Fresh Tofu and Herb Tofu had high bacterial counts (10⁵ cfu/g). Tofu Burger and Smoked Tofu had counts around 10⁴ cfu/g. Tofu Paste and Fried Tofu had low counts (<10² cfu/g). Cutting board, knife and frequent hand contact with product were the major sources of contamination for tofu. Herbs used for making Herb Tofu were highly contaminated (>10⁵ cfu/g). Cook water and cooked beans for tempeh production had lower counts (10⁴ cfu/g). Initial count of fresh tempeh was high (about 10⁸ cfu/g). Most products had counts of >10⁶ cfu/g after 7 days of cold storage. The microbiological quality of the product may improve if producers avoid unnecessary contamination during processing.     

Traditional production and chemical composition ofNdaleyi,a Nigerian fermented pearl millet food

Ndaleyi, a fermented, sun dried agglomerated powder produced from pearl millet or sorghum is one of the most popular foods consumed in Nigeria, mostly by the Kanuri people of Borno State. Its traditional production is described. The mean yields ofndaleyi (mainly starch),chir (millet gluten) and bran (overtail orbina) were 30.6, 22.5 and 21.5%, recpectively. A mean yield loss of 20% was observed. Chemical analysis revealed thatchir and bran have higher protein, fat and ash contents thanndaleyi. The titratable acidities (as percent lactic acid) ofndaleyi andchir were 0.9 and 0.3, while their pH values were 3.3 and 3.5, respectively.     

Production,nutritional profiles and storability of ‘aadun’ and ‘kokoro’: Two corn products

Aadun and kokoro were produced from maize grains by processes involving roasting, kneading, spicing, and frying. Aadun with a moisture content (MC) of 12.5%, pH 5.3 had the following composition: crude protein, 7.49%; fat, 28.5%; total carbohydrate, 60.0%; ash, 3.0% and energy value, 3,600 kcal/kg whilst kokoro with a MC of 16% and pH 5.1 had, respectively: 6.42%; 22.9%; 66.6%; 2.84% and 3,550 kcal/kg. Whilst aadun and kokoro stored under ambient conditions (28±2°C; 75–80% RH) became mouldy within 7 days, samples treated with 1% butyl paraben did not develop off-colour and mouldiness.     

Zusammenhänge zwischen den für die Blaufleckigkeit der Kartoffelknolle verantwortlichen Inhaltsstoffen

Zusammenfassung Mit der Faktorenanlyse nach der Hauptachsenmethode und der aufbauenden multiplen Regressionsanalyse, unter Einbeziehung von 56 Einflußgrößen, konnte gezeigt werden, daß die Blaufleckigkeit von Kartoffelknollen im wesentlichen vom Gehalt an Trockensubstanz, -Aminobuttersäure, Lysin, Isochlorogensäure und den Mineralstoffen Ca und K abhängt. Eine kausale Erklärung des gesamten Komplexes Blaufleckigkeit ist allerdings zum gegenwärtigen Zeitpunkt noch nicht möglich.

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Relationships between black spot of potatoes and several constituents of the tuber. With a factorial analysis and a multiple regression analysis could be shown that from 56 investigated constituents of the potato tuber only the dry matter content, aminobutyric acid, lysine, isochlorogenic acid, and potassium and calcium content influence the appearance of black spots.A causal explanation of the black spot phenomenon at present is not possible. Further investigations are necessary.

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Résumé Relations entre la tâche noire des pommes de terre et divers constituants du tubercule.L'analyse factorielle, l'analyse de la régression multiple ont pu démontrer que sur 56 constituants déterminés dans le tubercule de pomme de terre seuls l'acide aminobutyrique, la lysine, l'acide isochlorogénique, le taux de potassium et de calcium agissent sur la formation de la tâche noire.Une explication causale du phénomène tâche noire n'est pas encore possible pour l'instant. De nouvelles recherches sont nécessaires.

     

Influence of grain quality,heat, and processing time on the reduction of aflatoxin B1 levels in ‘tuwo’ and ‘ogi’: Two cereal-based products

During the production of tuwo from laboratory-contaminated corn (AFB₁:150 mcg/kg) and sorghum (AFB₁:87.5 mcg/kg) grains, reductions in the aflatoxin-B₁ levels of pastes boiled for 30 min and 60 min were found to be 68.0% and 80.8%, respectively. In the preparation of ogi from contaminated corn and sorghum grains, reductions of about 72.5% and 71.4%, respectively, were obtained after fermentation at ambient conditions. Reconstitution of ogi paste into a porridge (akamu) considerably reduced the AFB level.     

Effect of alkaline treatment (‘akanwu’) and supplementary value of corn or crayfish on the protein quality of breadfruit (Treculia Africana)

The effect of treatment and supplementary value of corn (C) or crayfish (CR) on the protein quality of breadfruit (Treculia Africana) flours were studied in eighteen young rats. The 7030 or 701515 (Protein basis) combinations of breadfruit flours and corn or crayfish or both provided 1.6g N/100 g diet for the 35 day study. The addition of akanwu to the cooking water reduced cooking time and crude protein and saved fuel. The addition of akanwu and replacement of CR with C was not beneficial as judged by the parameters tested except for the wt. gain and PER. On the other hand, when crayfish was the only source of supplementary protein (30%) to breadfruit cooked without akanwu there were increases in all parameters tested over those with added akanwu except for the N intake, wt. gain, and PER.These results appear to suggest that addition of akanwu to TA was detrimental to its protein utilization and that TA appears to be an economic source of N in areas where it is a staple. Based on the results of this study, one would suggest that the use of akanwu as a tenderizer should be seriously looked into before further use.     

Verbreitung des Wissens über die ‘innere’ Qualität von Gemüse und Obst in der Öffentlichkeit

Zusammenfassung Anhand praktischer Beispiele werden qualitätsrelevante Problemkreise vorgestellt und eingehend diskutiert, so Erzeugerberatung — Weg der Ware vom Erzeuger zum Verbraucher — Äußere Beschaffenheit — Innerer Wert — Präsentation der Verkaufsware — Erhaltung der Frische — Berücksichtigung ernährungsphysiologischer Kriterien. Es wird ein Publikationsmodell — bereits 1950 entwickelt — vorgeschlagen, das weite Kreise der Züchter, der Erzeuger, der Fabrikanten, des Handels und der interessierten Verbraucherschaft sowohl ansprechen als auch das Verständnis der einzelnen Gruppen untereinander wecken könnte.Denn letztlich sollte es doch im Bermühen des Erzeugers, des Transporteurs, des Handels, des Verarbeiters von Obst und Gemüse in erster Linie darum gehen, das Wohlergehen, die Gesunderhaltung des Verbrauchers im Auge zu haben. Das wäre jedenfalls die Vorstellung und Erwartung der ernährungsphysiologisch und ernährungs-hygienisch ausgerichteten Qualitätsforscher.

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Public extension about the inner Quality of Vegetables and Fruit

As usually practized in vegetable and fruit cultivation problems relevant to the inner quality are presented and discussed, e.g. Information of consumers, Shipping products from producer to consumer, External Quality — inner Quality — Offering of products — Conserving of freshness — Regarding the nutritional value.In order to evaluate these problems a close co-operation of breeders, producers, manufacturers, dealers and the interested consumers seem to be necessary. This is possible through a model and system of information, similar to the so-called AZA which were first published in 1950.

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Referat, gehalten auf der Sitzung der neukonstituierten Kommission Öffentlichkeitsarbeit der DGQ am 1.10.1976 in Geisenheim/Rhein.     

Evaluation of turmeric accessions for quality

One hundred and eighty four accessions ofCurcuma were analysed for curing percentage (dry recovery), essential oil, oleoresin and curcumin contents. Curing percentage varied from 13.5 to 32.4. The cultivar Konni had the maximum percentage of oleoresin (19.2). The volatile oil content was more inC. aromatica than inC. domestica. Curcumin content varied from 2.3% in cultivar Hahim to 10.9% in cultivar Edapalayam. However, curcumin content was comparatively lower in six exotic types as well as in 14 related species. The importance of quality aspects with respect to breeding is discussed.Contribution No. 493 of Central Plantation Crops Research Institute, Kasaragod-670 124, Kerala, India.     

Isolation and Partial Characterization of Mango (Magnifera indica L.) Starch:Morphological,Physicochemical and Functional Studies

Mango (Mangifera indica) is a fruit that grows in tropical regions. The aim of this work was to isolate the starch from two varieties of mango highly consumed in Mexico (criollo and manila), and to evaluate its chemical composition, along with some morphological, physicochemical and functional properties. Mango starch had an amylose content of about 13%, the fat content of criollo variety starch (0.1–0.12%), was similar to that of commercial corn starch used as control (0.2%); both mango starches had higher ash amount (0.2–0.4%) than corn starch. Mango starches presented a smaller granule size (10 m) than corn starch (15 m), along with an A-type X-ray diffraction pattern with slight tendency to a C-type. All values of water retention capacity (WRC) increased with the temperature. When the temperature increased, solubility and swelling values increased and in general, mango starches had higher values than corn starch. Both mango starches had gelatinization temperatures lower than the control, butcriollo variety starch presented higher enthalpy values thanmanila variety and corn starches. Overall, it was concluded that due to its morphological, physicochemical and functional properties, mango starches could be a feasible starch source with adequate properties, suitable for using in the food industry.     

Developmental changes in composition and quality of prickly pear cactus cladodes (nopalitos)

The composition and quality of edible tender stems or cladodes of 3 Prickly Pear Cactus species (Opuntia amyclaea, O. ficus-indica, and O. inermis) were studied at different stages of development. This traditional Mexican vegetable is called nopalitos in Spanish and cactus leaves in English. Cladodes harvested when 20 cm in length have the following average composition per 100 g: 91.7 g of water, 1.1 g of protein, 0.2 g of lipid, 1.3 g of ash, 1.1 g of crude fiber, 4.6 g of complex carbohydrates and 0.82 g of simple sugars, 12.7 mg of ascorbic acid and 28.9 µg of carotenes. The cladode's juice has an average pH of 4.6, 0.45% titratable acidity and 6.9% soluble solids. The components which varied most during development of the cladodes were: carotenes, acidity and total carbohydrates which increased, and protein and crude fiber (acid-detergent) which decreased. The nutritive value of the tender cladodes in the stages of growth at which they are commonly harvested and consumed (15 to 25 cm long weighing 50 to 80 g per stem), was similar for the 3 species.     

Biochemical changes during the development of cork spot of apples

Biochemical changes occurring during the development of cork spot of York Imperial were investigated. At the first visible sign of the disorder, the rate of ethylene production increases in the affected tissue. Respiration also increases, acetate being the major respiratory substrate rather than glucose. Protein synthesis, pectin synthesis, and the movement of inorganic ions into the tissue follow. During the time the chemical changes are taking place in the tissue, abnormal cell division is initiated, packing the newly-formed cells into the intercellular spaces. At the final stage of development, the tissue becomes brown and appears as a firm brown spot in the flesh of the apple. Cork spot is somewhat different from bitter pit in that the spots appear early in the season, the affected tissue is deeper in the flesh, and firmer. The chemical changes discovered so far in both disorders, however, appear to be similar. We consider the abnormal chemical changes that occur in both disorders to be common to diseases and injuries and not specific for either cork spot or bitter pit.

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Zusammenfassung Die Untersuchungen erstreckten sich auf biochemische Veränderungen, die während der Entwicklung von cork spot bei Äpfeln der Sorte York Imperial auftreten. Beim ersten sichtbaren Anzeichen der Störung steigt die Rate der Äthylenbildung im befallenen Gewebe an. Auch die Atmung nimmt zu, wobei Acetat das grössere Atmungsprodukt darstellt als Glucose. Protein- und Pektin-Synthese sowie Bewegung anorganischer Ionen in das Gewebe folgen. Während der Zeit chemischer Veränderungen im Gewebe beginnt eine abnorme Zellteilung, indem die neugebildeten Zellen in die interzellularen Zwischenräume eingebaut werden. Im Endstadium der Entwicklung wird das Gewebe braun und erscheint als fester Fleck im Fleisch des Apfels. Cork spot unterscheidet sich etwas von der Stippigkeit dadurch, dass die Flecken früher in der Saison erscheinen, sich die befallenen Gewebe tiefer im Fleisch befinden und fester sind. Die chemischen Veränderungen beider Störungen scheinen sich — soweit sie bekannt sind — zu ähneln. Wir glauben, dass die chemischen Veränderungen, die bei beiden Störungen auftreten, allgemein für Krankheiten und Verletzungen zutreffen und nicht entweder für Stippigkeit oder cork spot spezifisch sind.

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Resume Quelques changements biochimiques se produisant pendant le développement du cork spot chez la York Imperial ont été étudiés.Au premier signe visible de désordre, la vitesse de production d'éthylène augmente dans le tissu atteint. La respiration augmente également; l'acétate devient le substrat principal de la respiration, plutôt que le glucose. Une synthèse de protéines, de pectine, ainsi que des déplacements d'ions inorganiques dans le tissu se produisent ensuite.Pendant que les changements biochimiques ont lieu, une division cellulaire anormale est initiée, et les cellules nouvellement formées s'accumulent dans les espaces intercellulaires.Au stade final de développement, le tissu devient brun et apparaît comme une tache brune et ferme, dans la chair de la pomme. Le cork spot diffère quelque peu du bitter pit par le fait que les taches apparaissent tôt dans la saison, le tissu malade étant localisé plus profondément dans la chair, et plus ferme. Les modifications chimiques trouvées dans les deux maladies paraissent cependant être similaires. Nous considérons ces changements chimiques comme propres aux maladies et blessures en général et comme non spécifiques, ni du cork spot, ni du bitter pit en particulier.

     

Commercial soybean starch blocker consumption: Impact on weight gain and on copper,lead and zinc status of rats

A commercial starch blocker was used to study the digestin of starch (potato) in mature female rates for four weeks. Two levels of starch blocker were used. The first level was calculated to inhibit starch digestion at 50%, the second was calculated to inhibit starch digestion at 100%. No significant effects on the body weights (271.10±29, 277.7±43, 259.1±25 g) were found among the groups of rats at 0%, 50% and 100% inhibition levels, respectively. Feed intakes were not affected. However, fecal copper and zinc excretions increased significantly (p<0.05) due to the inhibitors. Fecal copper excretions were 0.468±0.14, 0.578±0.09, 0.617±0.07 mg/rat/week, while fecal zinc values were 0.625±0.14, 0.859±0.32 and 0.778±0.26 mg/rat/week when no inhibitor was fed, at 50% inhibition and at 100% inhibition respectively. Thus, while use of starch blockers did not promote weight loss in the mature female rats, utilization of copper and zinc were negatively affected.Published as Nebraska Agricultural Research Division Project Journal Series No. 8791. Supported by funds from the Nebraska Agricultural Division Project No. 91-031 and U.S.D.A. C.S.R.S. Project No. W-143.     

Fluorodensitometric estimation of cucurbitacin-C in leaves ofCucumis sativus L.

A quantitative method for the determination of cucurbitacin-C inCucumis sativus L. is presented. Crude chloroform extracts of the leaves were subjected to thin layer chromatography (TLC). After treatment with antimony trichloride and heating, the fluorescence of the spots was measured with a flying spot densitometer.     

Comparative studies on the nutritional and toxic composition of three varieties of Lesianthera africana

The research presents analytical data on three varieties of a commonly consumed vegetable, Lesianthera africana, in southern Nigeria. The Etoi (bitter) variety seemed to possess the best (p0.01) nutritional value (highest lipid, protein, calories, iron, calcium, phosphorus, least fiber) but highest toxic components (mg/100 g: 1.62 HCN; 17.6 oxalate; 1630 glucosinolates), which are far below toxic limits. The Ikot Abasi (riverine) variety was found to be highly (p0.01) fibrous (18.1 mg/100 g dry matter) but low in micronutrient minerals. The Etinan (flavorful) variety was found to be moderately nutritious. The findings are discussed in relation to nutrient interactions and recommendations are made regarding dietetic use.     

Nutritive characterization of purslane accessions as influenced by planting date

The uniqueness of purslane (Portulaca spp.) asthe richest vegetable source of omega-3 (-3)fatty acids is well documented. However, purslane hasnot been domesticated or fully evaluated for itsnutritive value. The objective of this study was todetermine the influence of planting date on chemicalcomposition of purslane accessions. Eight accessionsfrom different geographical locations were planted 12days apart, and whole plants harvested at full bloom. Chemical analysis (DM basis) of leaves showedsignificant differences among varieties for all thecharacteristics measured. Accession by planting dateinteraction influenced (p < 0.05) levels of crudeprotein, total lipids, and carbohydrate contents. Wild Greek accession had the highest, while aBeltsville (Maryland) wild type had the lowest crudeprotein content (27.1 vs 20.5%) at the secondplanting date. Crude protein, lipid and ash levelswere most influenced (p < 0.05) by planting date. Total lipids varied from 4.0–5.8% and 3.7–5.1% forthe first and second planting dates, respectively. Selected fatty acid content indicated significantly(p < 0.05) higher levels of 18: 26, and18: 33 in the Dutch Garden accession comparedwith other varieties. The Egyptian wild accession hadthe lowest level of 18: 33. The ratio of 3 to 6 acids, which ranged from 5.5 to22.3 indicated a highnutritive value of purslane compared to other oilcrops such as soybeans and perrilla. The high levelsof protein in purslane compete with those of othercommercially important vegetable crops. The studyshows that, in spite of its genetic diversity, purslaneremains one of the most abundant terrestrial vegetablesources of Omega-3 fatty acids and other essentialnutrients potentially beneficial for humans as well asanimals.     

‘Translocation’ — Toxicity ought to be considered in tests for pesticide registration

Summary Objections are raised against the present mode of action in the toxicological evaluation of new pesticides which are subsequently introduced commercially.These obsolete methods are not safe unless they consider the so called translocation-toxicity.This term is defined and its justification verified by a series of experimental data in toxicological research.

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Zusammenfassung Gegen die heutigen toxikologischen Verfahren bei der amtlichen Prüfung und Zulassung neuer Pestizide werden entschiedene Vorbehalte angemeldet.Diese antiquierten Methoden gewähren keine gesundheitliche Unbedenklichkeit, es sei denn sie würden die Prüfung auf die sogen. Translokations-Toxizität miteinbeziehen.Dieser Begriff wird definiert und seine Berechtigung durch eine Reihe experimenteller Daten aus toxikologischen Versuchen belegt.

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Résumé Les méthods actuelles de contrôle officiel et d'admission de nouveaux pesticides soulèvent des réserves très graves.Ces méthodes anciennes ne donnent aucune garantie de sécurité hygiénique même si elles portaient aussi sur la soi disante toxicité translocatoire.Cette notion est ici définie; elle est justifiée par une série de données expérimentales et d'essais toxicologiques.

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Research of the Deutsche Gesellschaft für Qualitätsforschung (pflanzliche Nahrungsmittel),D-6222 Geisenheim/Rhg, West Germany.     

Qualitative and quantitative changes in the seed and oil content ofBrassica juncea mutants at different times of harvesting

With increasing maturity, the moisture percentage, a, b, and total chlorophyll and crude fibre content decreased progressively, whereas oil and protein content increased. However, 1000-grain weight and pod yield increased up to stage II. Some significant correlations observed in green pod stage I were absent in the pale yellow stage II and golden yellow stage III of maturity. The positive significant correlations between 1000-grain weight and seed yield existed in both stages I and II, but there was no correlation between these characters at the golden yellow stage III. Thus mustard can be harvested at the pale yellow pod stage without any adverse effect on seed quality and yield.Presented at the 15th Annual Workshop cum Seminar on rapeseed and mustard of the All-India Coordinated Research Project on Rabi oilseed crops held at CSA University of Agriculture and Technology, Kanpur, India, 12–13 September 1979.     

Development and Acceptability of a Ready-to-Eat Β-Carotene Rich,Maize Based Supplementary Product

Low cost supplementary products using maize were developed and made using extrusion. -carotene rich sources like curry leaf, carrot, red palm oil were used at different level to increase vitamin A precursor Levels and, therefore, vitamin A. Incorporation of curry leaf powder and carrot powder at 30 percent level and 30:70 blend of red palm oil and groundnut oil were found to be more acceptable than the products made with other levels. These products were tasted for acceptability by preschool children and were analysed for energy, protein, fat and -carotene contents. The control, fresh and stored supplementary products contained 1.707, 1.922, 1.919 MJ, 11.0, 11.6, 10.36 g protein, 10.2, 10.4, 9.64 g fat, 0, 7.37,6.72 mg -carotene per 100g, respectively. The loss of -carotene in processing and storage of curry leaf and carrot supplemented products was 13.69, 6.25 and 20.24, 8.06 percent, respectively.     

Beta-carotene content of postharvest orange-fleshed muskmelon fruit: Effect of cultivar,growing location and fruit size

The influence of two growing locations (soil types), six fruit sizes, and two years on the postharvestBeta-carotene content of muskmelon (Cucumis melo L. var. reticulatus Naud.) fruit was studied with two different cultivars. Fully abscised commercial size fruit: 9, 12, 15, 18, 23, and 30 (fruit/0.04 M³ shipping box) had highly variableBeta-carotene contents (5.3 to 33.8 µg/g fresh weight) that varied by size class, soil type and cultivar.Beta-carotene content increased with fruit size up to a maximum, though fruit size continued to increase. Find sandy loam soil produced fruit with lessBeta-carotene content than silty clay loam soil. The cultivar Primo contained higherBeta-carotene content levels than cultivar Cruiser. Mesocarp percent moisture content for both Cruiser and Primo at both locations by fruit size was not significantly correlated (r=0.40) withBeta-carotene content. Indicating fruit cell dilution may not contribute to the differences inBeta-carotene content in different fruit size classes.Beta-carotene content of size class 18 fruit from six cultivars grown on the silty clay loam soil for two consecutive years, showed a year, and year by cultivar effect for some cultivars. Whereas, some cultivars did not differ inBeta-carotene content between the two years. This indicates a potential for controllingBeta-carotene content of muskmelon fruit at a constant, high level by careful selection of production cultivar.Mention of a trademark of propriety does not constitute a guarantee or warranty of the product by the US Department of Agriculture or Texas A&M University and does not imply its approval to the exclusion of other products that also may be suitable.