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苹果RAPD分析体系的建立 总被引:3,自引:0,他引:3
对富士苹果(Malus pumila Mill.cv.Fuji)RAPD(Random Amplified Polymorphic DNA)分析体系的优化研究表明,RAPD反应体系中,DNA、Taq酶、引物和Mg2+4种主要成分的最适用量分别为:20ng、1.0 U、0.2umol·L-1和3.0umol·L-1。采用该优化体系,以 OPJ03为引物,构建了我国及世界范围内苹果生产中重要的28个品种的RAPD指纹图谱,分析了其遗传多样性,区分了供试的28个苹果品种中的15个,区分率达53.6%。讨论了RAPD鉴定苹果品种的应用及其主要影响因素。 相似文献
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RAPD技术在金针菇杂交育种中的应用 总被引:12,自引:0,他引:12
本文以金针菇不同亲本菌株及其杂交种为材料,用20个随机引物进行了RAPD分析,结果表明,两个随机引和的扩增出特异的DNA片段,经三次重复,均获得相同结果,且能明显地区别鉴定出真正的杂交种,因此,在杂交育种中,RAPD分析是一种简便、快速、有效地从杂交后代中区别鉴定出真正杂交种的方法。 相似文献
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苹果RAPD分析体系的建立 总被引:16,自引:0,他引:16
对富士苹果(Malus Pumila Mill.cv.Fuji)RAPD(Random Amplified Polymorphic DNA)分析体系的优化研究表明,RAPD反应体系中,DNA、Taq酶、引物和Mg^2+4种主要成分的最适用量分别为:20ng、1.0U、0.2μmol.L^-1和3.0μmol.L^-1。采用该优化体系,以OPJ03为引物,构建了我国及世界范围内苹果生产中重要的28个 相似文献
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分子生物学技术在菇类研究中的应用 总被引:6,自引:1,他引:5
本文报道了应用RFLPs和RAPD技术分析香菇类缘关系,鉴别品系及分析木耳杂交后人攻原体质体融合子的结果,发现供试的32个香菇菌株的总DNA限制性内切酶片段长度存在多态性;木耳杂交后代、融合子及其亲株间DNA PCR扩增产物谱带型出现差别,这充分说明RFLPs和RAPD技术在分析遗传变异、鉴别品系、鉴定杂种和融合子研究中的重要作用。 相似文献
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厚皮甜瓜亲缘关系及纯度的RAPD标记 总被引:4,自引:0,他引:4
利用20个随机引物对厚皮甜瓜8个亲本与4个杂交种进行RAPD分析。10个引物的扩增DNA谱带具有多态性,亲本具有各自的特异谱带,绝大部分F1的扩增带型是双亲的互补带。各试材的相似系数范围为63.6%-96.5%。遗传距离聚类分析图表明,F1易与双亲或其一聚在一起。上述结果说明,可以利用RAPD标记在DNA水平上进行厚皮甜瓜的亲缘关系及纯度的鉴定。 相似文献
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黑籽南瓜DNA导入西瓜后子代RAPD标记的变化 总被引:10,自引:0,他引:10
应用花粉管通道导入技术将黑籽南瓜DNA导入受体早花西瓜,经两次传代后,得到有变异性状的子代D2-1、D2-2、D2-3,对三个子代及原始亲本黑籽南瓜和早花西瓜进行了RAPD分析。 相似文献
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PEG渗调引发处理对菜薹老化种子DNA损伤的修复作用 总被引:7,自引:0,他引:7
利用RAPD技术对菜薹人工老化种子DNA的损伤及PEG渗调引发处理的修复作用进行了研究。在61种引物中,有6种在菜薹人工老化的种子上获得了基因组DNA指纹图谱,平均每种引物扩增的总DNA带数为2.16,多态性DNA带数为1.17,有11种引物在PEG渗调引发的菜薹老化种子上获得了基因组DNA指纹图谱,平均每种引物扩增的总DNA带数为3.91,多态性DNA带数也是3.91。人工老化对菜薹种子基因组D 相似文献
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应用RAPD技术对香菇融合菌株进行遗传鉴定 总被引:10,自引:1,他引:9
本文应用RAPD技术与聚类分析方法,对香菇细胞融合菌株F1及亲本S1、465进行遗传分析.根据DNA扩增的指纹图谱,估算融合子与亲本的DNA相似系数并构建遗传相关性聚类图.结果表明,RAPD技术与聚类分析方法可应用于香菇细胞融合菌株的遗传鉴定. 相似文献
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Anuj P. Srivastava Ramesh Chandra Sangeeta Saxena Shailendra Rajan Shirish A. Ranade 《The Journal of Horticultural Science and Biotechnology》2013,88(6):951-959
SummaryThree different PCR methods [Random Amplified Polymorphic DNA (RAPD), Inter-Simple Sequence Repeats (ISSR), and Directed Amplification of Minisatellite DNA (DAMD)] were used to analyse genetic diversity and parentage among 20 mango cultivars, including 18 landraces and two hybrids (‘Amrapali’ and ‘Mallika’). These hybrids together with a third hybrid (‘Ratna’), and an out-group species (Mangifera sylvatica) were also analysed for parentage. Fifteen, seven and four primers were used to amplify a total of 158, 69 and 59 distinct DNA fragments by RAPD, ISSR and DAMD, respectively. Of these, approx. 85%, 64% and 90% were polymorphic, respectively. Genetic distances between pairs of mango cultivars were measured separately by each method and depicted graphically as a Neighbor Joining (NJ) tree. The three methods revealed different groupings of cultivars and hybrids. A NJ tree based on the cumulative data from all methods correlated well with the parentage of the mango hybrids, and the grouping of cultivars on a regional basis. Genetic markers likely to be associated with important agronomic traits were identified by further analysing the hybrids, with their respective parents, using all three methods. On the basis of the highest number of polymorphic bands observed (90%), DAMD was judged to be the best method with which to analyse mango germplasm. 相似文献
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《Scientia Horticulturae》2005,105(1):53-64
The segregation ratio of morphological characters (15 quantitative and 4 qualitative) and random amplified polymorphic DNA (RAPD) markers in 55 interspecific hybrids between Dianthus giganteus and D. carthusianorum and their parents were studied. Interspecific hybrids exhibited either intermediate characteristics between parents shorter, fewer characteristics than parents except for stem length and height of corolla where heterosis was found. Segregation of four qualitative characters such as profile of the lower part of the corolla, stigma color, the main secondary color of petal blade, and arrangement of individual flowers, were exhibited as either characteristics of female parent or male parent.Two hundred and sixteen polymorphic RAPD bands detected in 55 interspecific hybrids were divided into four types: AB type (both-parental type) which was presented in both female parent and male parent, Ab type (female parental type) which was presented in only female parent, aB type (male parental type) which was presented in only male parent, and ab type (non-parental type) which was presented in only hybrids except their parents. The number of AB, Ab, aB, and ab type bands were 33 (15.3%), 56 (26.0%), 50 (23.1%), and 77 (35.6%), respectively. The proportion of parental type bands to non-parental type bands was two to one and only 54.6% of four type bands coincided with expected ratio. 相似文献
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甜瓜分子标记纯度鉴定研究 总被引:4,自引:1,他引:4
以2个甜瓜品种西域雪1号、西域雪2号及其亲本为试材,建立并优化了单粒种子DNA快速提取法和适合甜瓜的RAPD、SSR分子标记杂交种纯度鉴定体系。从100条随机引物中筛选出G17用于西域雪2号纯度鉴定,可有效区分母本和杂交种。从19对SSR引物中筛选出9对稳定扩增、多态性丰富的引物构建西域雪1号、西域雪2号的SSR指纹图谱。SSR分子标记用较少引物即可扩增出品种特异性条带,且多数F1代带型呈双亲互补,非常适合种子纯度鉴定。同时,使用高浓度、非变性的聚丙烯酰胺凝胶电泳、银染,条带更清晰,可区分只相差1个碱基的扩增片段,同RAPD相比,SSR分子标记更适合甜瓜杂交种纯度快速鉴定。 相似文献
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西瓜杂种及其亲本同工酶分析 总被引:9,自引:0,他引:9
以西瓜的10个杂种及其16个亲本为试材分析了干种子、发芽期、子叶期的根及子叶的过氧化物酶(POD)同工酶和酯酶(EST)同工酶。结果表明西瓜POD同工酶和EST同工酶酶谱在不同时期,不同部位不尽相同。发芽期及子叶期POD同工酶和子叶期EST同工酶存在着杂种与亲本差异,杂种酶谱表现为父本相同、母本相同、双亲相同、出现“杂种酶”带及缺少双亲某些酶带五种类型。其中POD同工酶与其父本相同和EST同工酶与其母本或父本相同的杂种多表现较强的杂种优势。 相似文献