鸡肾型传染性支气管炎的诊断及防洽

<正>鸡传染性支气管炎是由传染性支气管炎病毒引起鸡的一种急性、高度接触性传染病,可危害各种年龄的鸡,发病率较高,以1～40日龄的雏鸡发病最为严重,死亡率也高。该病主要侵害鸡的呼吸系统、泌尿生殖系统,尤其以肾型传染性支气管炎的发生最为常     

鸡传染性腺胃炎的诊断与防制措施

芒市风平镇是德宏州现代化养鸡场最集中的乡镇,现有8家建有鸡舍的养鸡场,鸡传染病的防控一直是每个鸡场的重点工作。鸡传染性腺胃炎是鸡传染性支气管炎(IB)的腺胃型病症。鸡传染性支气管炎是由传染性支气管炎病毒引起的一种急性、高度接触性呼吸道疾病。该病不同的毒株感染后,可出现如呼吸型、肾型、腺胃型等不同的临床症状。过去在德宏州未曾发现过腺胃型的鸡传染性支气管炎病例,2007年由于向外引鸡而引入该病,导致近年来德宏州部分养鸡场零星发生此病。     

浅谈鸡传染性腺胃炎的发生与防制措施

鸡传染性腺胃炎是鸡传染性支气管炎(IB)的腺胃型病症,是由传染性支气管炎病毒引起的一种急性、高度接触性呼吸道疾病。该病不同的毒株感染后,可出现如呼吸型、肾型、腺胃型等不同的临床症状。过去在德宏州未曾发现过腺胃型的鸡传染性支气管炎病例,因该州没有种鸡场,商品肉鸡和蛋鸡主要靠外地引进,2010年以来梁河、芒市的3个鸡场在引鸡而引发该病,给养鸡场造成一定的经济损失。     

鸡传染性支气管炎的诊断与控制

鸡传染性支气管炎(Infectious Bronchitis of Chickens,IB)是仅发生于鸡的急性、高度接触性呼吸道疾病.本病呈世界性分布.据报道,本病在临诊上分为腺胃型传染性支气管炎(腺胃型IB)、呼吸型传染性支气管炎(呼吸型IB)及肾型传染性支气管炎(肾型IB).     

鸡传染性支气管炎的诊断与科学防控

鸡传染性支气管炎是养鸡生产过程中常见的病毒病，主要发生于雏鸡群和产蛋鸡群，根据具体临床表现将该病分为呼吸生殖型和肾型2种，病鸡以呼吸道症状、产蛋性能下降和肾脏肿大为主要发病特征，临床危害较大。对鸡传染性支气管炎的病原、临床表现等加以阐述，提出通过抗继发感染和中药调理来治疗该病，以期为广大养鸡专业户提供防控参考。     

鸡传染性支气管炎肾脏型SK9株毒力稳定性试验报告

将鸡传染性支气管炎肾脏型SK9株通过鸡胚盲传10代,检测E1、E5、E10各代毒的病毒含量、安全性、免疫原性,用雏鸡回归5代,检测C1-C5各代毒的病毒含量、安全性等,说明SK9株是一株稳定性好的毒株。     

一起鸡肾型传染性支气管炎的诊断报告

江苏宿迁某鸡场发病鸡群分离到3株毒株,经鸡胚盲传2代后,鸡胚出现典型的侏儒胚变化。分离毒株对鸡的红细胞无凝集性,但用1%胰蛋白处理后,表现出不同程度的血凝性,雪凝价达7log2～9log2;毒株接种15日龄非免疫来航鸡,复制出典型的肾型传染性支气管炎的特征性症状及病变。经过上述实验室诊断,确诊为肾型传染性支气管炎,并采取相应的防治措施,取得了一定的效果。     

鸡呼吸型传染性支气管炎的药物防治

正鸡传染性支气管炎是由传染性支气管炎病毒引起的一种急性、高度接触性呼吸道疾病。本病不同的毒株感染后,可出现如呼吸型、肾型、腺胃型等不同的临床症状。近年来。云南省德宏州养鸡集中的芒市、陇川县、盈江县等一些从事土鸡育雏的养殖户不断带病死鸡到所在乡镇或市动物疫病预防控制中心就诊。据养殖户反映,鸡群一般在10日     

鸡肾型传染性支气管炎的研究进展

鸡传染性支气管炎是由冠状病毒引起的一种传染病,由于冠状病毒的病原基因型变化很多,近年来所引起的症状和病理变化有很大的差异,同时各血清型之间的免疫学反应也不完全一致,在临床上表现为肾型、呼吸道性、肠道等症状,以下对肾型传染性支气管炎进行综述。     

鸡传染性支气管炎组织嗜性的研究进展

鸡传染性支气管炎(AvianInfectiousBronchitis,IB)是由鸡传染性支气管炎病毒(InfectiousBronchitisvirus,IBV)引起的鸡的一种急性、高度接触性呼吸道疾病。自1982年邝荣禄报告了肾型IB以来,国内IB的发生已经遍布华北、华南、西北、西南和东北等大部分省、市和自治区。IBV抗原性异常复杂,不同毒株的结构是不均的,在毒力、致病性和组织嗜性上存在着很大差异。本文对目前国际公认的由IBV所致疾病的致病型(主要包括呼吸型、肾型、产蛋下降型及肠型,腺胃型)进行了综述,并从基因水平探讨了决定IBV组织嗜性的因素。     

翡翠贻贝多糖抑制流感病毒在鸡胚中增殖的研究

采用血凝滴度法研究翡翠贻贝多糖(PVPS)对鸡胚培养甲型流感病毒的抑制作用;通过联合给药试验研究PVPS对利巴韦林抗流感病毒的协同效应。与病毒对照组比较,PVPS各剂量组的血凝滴度降低差异极显著(P<0.01)。与利巴韦林组及同剂量PVPS单独给药组相比,联合给药组的血凝滴度降低差异极显著(P<0.01),且0.85相似文献   

Infectious pancreatic necrosis virus of salmonids: biological and antigenic features of a pathogenic strain and of a non-pathogenic variant selected in RTG-2 cells

Abstract. A non-pathogenic cell culture adapted variant was obtained from a normally pathogenic strain of IPN virus (Sp type) after several passages in RTG-2 cells. This cell culture modified (CCA) strain was compared with the original wild (W) strain in various tests. Cross neutralization tests showed no obvious antigenic difference between the two. However the CCA strain was neutralized by a 1:5000 dilution of normal trout serum whereas the W strain was not. In RTG-2 cells, CCA strain gave both large and small plaques, whereas the W strain gave only small ones. Both virus strains were heat sensitive and labile to cyclic freezing and thawing. The CCA virus was more stable in storage at 4°C under different pH conditions and its growth in RTG-2 cells was more rapid. The rt (supraoptimal temperature at which viral yield is depressed by 90%) appeared to be 19-20°C for the CCA strain and 18-19°C for the W strain. Pre-treatment of RTG-2 cells with ultraviolet inactivated CCA virus could inhibit growth of W virus, but had no effect on replication of homologous virus.     

鸡干扰素生产条件的比较研究〈一〉

本试验用鸡白细胞。鸡脾细胞、鸡胚成纤维细胞以Ge－132、NDV－F、PHA、PolyI:C为诱生剂,诱生外源性IFN,将鸡胚成纤维细胞—水泡性口炎病毒作鸡IFN的检测系统,对不同种细胞、不同的细胞浓度、不同的IFN诱生剂及培养方法等诸因素对IFN产生的影响进行了比较：研究了Ge-1322、NDV－F、PHA、PolyI：C在鸡胚成纤维细胞上的最佳诱生剂量;同时也进行了不同诱生剂在不同细胞上产生的IFN的理化性状研究,结果表明：（1）在Ge－132、NDV－F、PHA、PolyI：C四种诱生剂中,诱生能力差异极显著（P＜0.01）,且以Ge－132诱生IFN的能力为最强,其他依次为NDV－F、PolyI：CPHA（2）鸡脾细胞和鸡白细胞产生的IFN效价高于鸡胚成纤维细胞;（3）鸡脾细胞和鸡白细胞悬浮培养比静置培养产生的IFN效价高近1倍;（4）雏鸡脾细胞比成鸡脾细胞产生的IFN效价高;（5）未免疫鸡鸡胚成纤维细胞比免疫鸡鸡胚成纤维细胞产生IFN效价高;（6）鸡胚成纤维细胞在IFN诱生剂的作用下,4h即可产生IFN,20－24H达到最高产量;（7）IFN诱生剂最佳诱生剂量;鸡NDV－F为128HAU／ml;Ge－132为70ug／mlPolyI：C为50ug／ml;PHA为40ug／ml     

荆防败毒颗粒抑制新城疫Lasota株病毒的研究

为探讨荆防败毒颗粒对NDV活性影响,采用鸡胚法研究了荆防败毒颗粒在鸡胚上对新城疫病毒的作用,即先接种病毒再接种药、先接种药再接种病毒2种方式,并设试验对照组。分别收取尿囊液测定血凝效价(HA)。结果表明,荆防败毒颗粒在2种方式中对NDV影响较强。为ND的临床防治提供理论依据。     

An antigenic comparison of three strains of infectious pancreatic necrosis virus of salmonid fishes

Three strains of infectious pancreatic necrosis (IPN) virus of salmonid fishes were found to be closely related antigenically when compared by cross plaque neutralization. They differed significantly, however, in stability during freezing and storage at ?60°C. The American reference strain VR-299, which was the most stable, required consistently less antibody for a given degree of neutralization than the other two, regardless of whether the antiserum was specific for that strain or for either of the others. The CTT strain was the most unstable of the three, and required the greatest amount of antibody for a comparable degree of neutralization. The COHO strain was intermediate both in stability and in antibody requirement.The results seem to indicate that the CTT virus required the most antibody because it contained the largest amount of non-infectious virus (to chinook salmon embryo cells), which would still bind antibody and render it unavailable for neutralizing activity. The VR-299 virus contained the least amount of non-infectious virus, and consequently required the smallest amount of antibody.It is suggested that the relative stability of viral strains and possible variation in the content of non-infectious virus should be taken into account or controlled when quantitative neutralization data are used to differentiate or identify viral serotypes.     

Egtved virus: temperature-dependent immune response of trout to infection with low-virulence virus

Abstract. The present report deals with experiments concerning the theoretical basis for development of a live, attenuated vaccine against viral haemorrhagic septicaemia (VHS) of rainbow trout, Salmo gairdneri Richardson, on the basis of a virus strain derived from the Egtved virus reference strain F, the pathogenicity of which has been strongly reduced by in vitro passage. It is shown that the low pathogenicity of the virus (designated the Reva strain) is a genetically stable feature and that the protection against VHS induced by infection with the virus is due to an immune response. Following immunological priming at 10°C partial protection developed at 5° 10° and 15° but not at 20°C, whereas neutralizing antibody was produced at all four temperatures by some of the fish. The duration of virus persistence in trout was found to be inversely proportional to the water temperature.     

A monoclonal antibody cross-reactive with three salmonid herpesviruses

Abstract. A monoclonal antibody cross-reactive with strain H-83 Oncorhynchus masou virus (OMV) and Herpesvirus salmonis has been prepared. Western blot analysis revealed that the monoclonal antibody, designated clo. 7, recognized the 16 000 (16K)-polypeptide of these herpesviruses but not the polypeptides of infectious pancreatic necrosis virus or infectious haematopoietic necrosis virus. Clo. 7 showed neither neutralizing activity against strain H-83 nor cytotoxicity to strain H-83-infected RTG-2 cells in the presence of complement. The purified antigen, recognized by clo. 7, from strain H-83 was a polypeptide with a molecular weight of 16K, and sensitive to proteolytic enzymes and sodium periodate, but not to neuraminidase and ethylether. The antigen was heat stable and stable at pH 5·0 – 9·0.     

抗犬瘟热高免卵黄抗体IHA检测方法的建立

目前检测卵黄抗体的方法很多,但IHA方法具有简便快速的优点,本研究的目的是为了建立抗犬瘟热高免卵黄抗体检测方法。采用犬瘟热病毒和犬细小病毒二联疫苗处理作为疫苗抗原,用犬瘟热病死犬的肝脏部分处理后作为组织抗原,制备敏化绵羊红细胞,用IHA方法确定了犬二联疫苗抗原和犬瘟热病毒组织抗原的最适含量。二联疫苗提取抗原的含量为0.7 mg/mL致敏红细胞时,与犬瘟热单克隆抗体反应显著,抗体效价为1∶256;而患犬瘟热松狮犬的肝脏组织抗原蛋白浓度为0.5mg/mL,与犬瘟热单克隆抗体反应显著,抗体效价为1∶128。经过IHA验证,制备的松狮犬瘟热肝组织抗原,蛋白含量0.5 mg/mL 1%致敏红细胞,对应的单抗、鸡血清均出现了凝集,阳性对照也出现凝集,阴性对照未出现凝集。建立的IHA抗体检测方法,为准确检测制备抗犬瘟热高免卵黄抗体的效价奠定了基础。