Rapid estimation of microbial biomass in grassland soils by ultra-violet absorbance

A reliable and simple technique for estimating soil microbial biomass (SMB) is essential if the role of microbes in many soil processes is to be quantified. Conventional techniques are notoriously time-consuming and unreproducible. A technique was investigated that uses the UV absorbance at 280 nm of 0.5 M K₂SO₄ extracts of fumigated and unfumigated soils to estimate the concentrations of carbon, nitrogen and phosphorus in the SMB. The procedure is based on the fact that compounds released after chloroform fumigation from lysed microbial cells absorb in the near UV region. Using 29 UK permanent grassland soils, with a wide range of organic matter (2.9–8.0%) and clay contents (22–68%), it was demonstrated that the increase in UV absorbance at 280 nm after soil fumigation was strongly correlated with the SMB C (r=0.92), SMB N (r=0.90) and SMB P (r=0.89), as determined by conventional methods. The soils contained a wide range of SMB C (412–3412 μg g⁻¹ dry soil), N (57–346 μg g⁻¹ dry soil) and P (31–239 μg g⁻¹ dry soil) concentrations. It was thus confirmed that the UV absorbance technique described was a rapid, simple, precise and relatively inexpensive method of estimating soil microbial biomass.     

Soil microbiological and chemical effects of a nitrogen-fixing shrub in poplar plantations in semi-arid region of Northeast China

Nitrogen (N)-fixing species have a function to enrich N in soil. Mixing N-fixing shrub species into poplar stands can be assumed as a measure to increase productivity while improving soil fertility. To verify this assumption and to understand the temporal influences of N-fixing shrub species mixed into poplar plantations on soil fertility, we investigated selected soil chemical and microbial properties in pure poplar (Populus × xiaozhuanica W. Y. Hsu et Liang) and mixed poplar–seabuckthorn (Hippophae rhamnoides L.) stands at ages of five and 15 years in a semi-arid region of Northeast China. Both stands at age of five have similar values of aboveground biomass, total soil organic C concentration, total N concentration, microbial biomass C, and metabolic quotient; however, at age of 15, these values except for soil metabolic quotient are significantly greater in mixed poplar–seabuckthorn stand than in pure poplar stand. The soil metabolic quotient is lower in the former stand than in the latter stand. Our results suggest that, in semi-arid regions, mixing N-fixing shrub species into poplar plantations can improve soil fertility in a long run rather than in a short term; therefore, mixing N-fixing shrub species into poplar stands is an option to improve soil fertility and increase productivity in a long run.     

Changes in chemical and biochemical soil properties induced by 11-yr repeated additions of different organic materials in maize-based forage systems

The repeated addition of organic materials to the soil greatly affects the physical, chemical and biological characteristics. In the present work, we analyzed changes in soil quality properties of the tilled layer caused by different agronomic managements of maize which supply different amounts of carbon (C) and nitrogen (N) through the addition of slurry, farmyard manure or plant residues. The agronomic history of the analyzed soils, which derived from a medium-term (11 yr) field experiment located in NW Italy, represents typical managements of maize for this region. The area is characterized by highly intensive agriculture, with consequent risks to soil degradation that could be limited by the efficient utilization of organic inputs and by recycling within cropping systems, the large amounts of manure that are produced from the many animal breeding farms in this region. We used a combination of both different chemical (soil organic C and total N) and biochemical indicators (potential soil respiration, potentially mineralizable N (PMN) and potential soil microbial biomass (SMB)). We considered the suitability of the selected biochemical indicators to describe the changes in soil characteristics resulting from the past management.The results showed that the application of the different organic materials, in addition to urea-N fertilizer, increased SOM contents and altered the selected soil biochemical properties compared with the unfertilized treatment, especially in the upper 15 cm of the 0?30 cm tilled soil layer. Farmyard manure applications caused the greatest increase in SOM content, PMN and potential SMB, whilst return of maize straw produced the largest increase in potential soil respiration, but had less effect on total soil organic C and SMB. The use of slurry only caused a moderate increase in SOM and showed intermediate changes in biochemical properties. Also, the rate of C accumulation in the soil per unit of C applied was higher for farmyard manure application than for slurry and straw incorporation in the soil. Fertilization with only mineral N did not induce an increase in C_org and N_tot and even reduces soil N mineralization potential.Because of the high variability in the data, potential SMB carbon could be considered as a less successful indicator for differentiating between past agronomic histories and effects on soil quality, whilst microbial activity (measured by potential soil respiration) and PMN, gave a more reliable and useful indication of the amount of easily decomposable organic carbon.     

Soil sampling based on field spatial variability of soil microbial indicators

Microbial indicators exhibit a high spatial heterogeneity which often masks comparison of the effects of different soil management treatments. It is therefore desirable to use a proper sampling design which integrates spatial heterogeneity at field level. Thus, the objectives were: (1) to study the spatial heterogeneity of biological and chemical soil variables, and (2) with obtained maps of spatial variability to test different sampling strategies to assess the usefulness of this ‘new’ soil map unit. 63 soil samples were collected according to a 10 m × 10 m grid on a 0.48 ha plot. On each of these samples, 10 variables were measured: soil water content, cation exchange capacity (CEC), organic carbon (OC) and total nitrogen (N), C/N, soil microbial biomass (SMB), labile soil organic matter (LOM), mineralisable C (C_min) and N (N_min), inverse of specific respiratory activity (1/SRA = SBM/C_min). The spatial heterogeneity of each variable was charted with geostatistics. The biological variables exhibited spatial variability of the same order of magnitude as physicochemical parameters. From the maps, zones with different levels of organic matter, microbial biomass and specific respiratory activity were identified. The spatial patterns of SMB and SRA were related to CEC (positively for SMB and negatively for SRA), pointing out the effects of soil protection on microbial biomass and availability of organic substrates. The definition of these zones for a pool of variables (OC, N, SMB, LOM, CEC) is useful at 3 levels: (1) to record the initial values of the measured variables for each plot, (2) to integrate these zones as blocks in experimental design for future experiments, and (3) to focus analysis of specific biological mechanisms such as activity of micro-organisms.     

Natural abundance radiocarbon in soil microbial biomass: Results from a glacial foreland

We present a method for determining the natural abundance radiocarbon (¹⁴C) content of soil microbial biomass (SMB) based on existing fumigation-extraction procedures. We applied the technique to soils from the foreland of the Ödenwinkelkees glacier in the Austrian Alps, which has a well-characterised chronosequence of soils at different stages of development. Across the chronosequence, SMB contained post-bomb levels of ¹⁴C, suggesting it was substantially composed of carbon that had been fixed since the 1960s. Comparison of our results with previous findings from the same site showed that at most stages in the sequence the SMB had a similar ¹⁴C content to the bulk soil organic matter (SOM). However, soil respired CO₂ was ¹⁴C-depleted relative to SMB, indicating that at least a component of the microbial community was mineralising some older carbon. In the most recently exposed soils, SMB was ¹⁴C-enriched compared to both soil respiration and SOM, suggesting that a small component of the microbial biomass that utilises older carbon contributes disproportionately more to the CO₂ efflux. Although other interpretations are possible, this explanation is consistent with the notion that early on in the succession a large proportion of the microbial biomass is dormant.     

Soil carbon and nutrient pools, microbial properties and gross nitrogen transformations in adjacent natural forest and hoop pine plantations of subtropical Australia

Background, Aims, and Scope  An improved understanding of important soil carbon (C) and nutrient pools as well as microbial activities in forest ecosystems is required for developing effective forest management regimes underpinning forest productivity and sustainability. Forest types and management practices can have significant impacts on soil C and nutrient pools as well as biological properties in forest ecosystems. Soil C and nutrient pools were assessed for adjacent natural forest (NF), first rotation (1R) (50-year-old), and second rotation (2R) (1-year-old) hoop pine (Araucaria cunninghamii Ait. ex D. Don) plantations in southeast Queensland of subtropical Australia. Materials and Methods  Five transects spaced 3 m apart with 9 sampling points along each transect were selected (9.6 m × 12.0 m each site), with 45 soil cores (7.5 cm in diameter) collected and separated into 0–10 and 10–20 cm depths. These soils were analysed for total C, total nitrogen (N), C (δ¹³C) and N (δ¹⁵N) isotope composition. The 0–10 cm soils were analysed for pH, CEC, exchangeable cations, total P and total K, and assayed for microbial biomass C and N, respiration, metabolic quotient, potential mineralizable N (PMN), gross N mineralization (M) and immobilization (I). Results  Total C and N in 0–10 cm soils were higher under NF and 1R plantation than under 2R plantation, while they were highest in 10–20 cm soils under NF, followed by the 1R and then 2R plantation. δ¹³C was lower under NF than under the plantations, while δ¹⁵N was higher under NF than under the plantations. Total P was the highest under NF, followed by the 1R and then 2R plantation, while total K was higher under the 2R plantation. No significant differences were detected for pH, CEC, exchangeable cations, microbial C and N, respiration and metabolic quotient among the 3 sites. PMN and M were higher under NF, while I was the highest under the 2R plantation, followed by the NF and then 1R plantation. Discussion  Soil total C and N in 0–10 cm depth were significantly lower under 2R hoop pine plantation than those under NF and 1R hoop pine plantation. There were significant reductions in soil total C and N from NF to 1R and from 1R to 2R hoop pine plantations in 10–20 cm depth. This highlights potential N deficiency in the 2R hoop pine plantations, and application of N fertilizers may be required to improve the productivity of 2R hoop pine plantations. There were no significant differences in other soil chemical and physical properties in 0–10 cm depth among the 3 sites under NF, 1R and 2R hoop pine plantations, except for soil total P and K. Soil microbial biomass C, CO₂ respiration and metabolic quotient did not differ among the 3 sites assessed, perhaps mainly due to these biological variables being too sensitive to variations in soil chemical and physical properties and thereby being associated with a larger variability in the soil biological properties. However, soil potential mineralizable N, gross N mineralization and immobilization were rather sensitive to the conversion of NF to hoop pine plantation and forest management practices. Conclusions  Total C and N in the top 20 cm soil were highest under NF, followed by 1R and then 2R hoop pine plantations, indicating that N deficiency may become a growth-limiting factor in the 2R hoop pine plantations and subsequent rotations of hoop pine plantation. The sample size for soil δ¹³C seems to be much smaller than those for soil total C and N as well as δ¹⁵N. The significant reductions in soil total P from NF to 1R and then from 1R to 2R hoop pine plantations highlight that P deficiency might become another growth-limiting factor in the second and subsequent rotations of hoop pine plantations. Soil microbial properties may be associated with large spatial variations due to these biological properties being too sensitive to the variations in soil chemical and physical properties in these forest ecosystems. Recommendations and Perspectives  Soil potential mineralizable N, gross N mineralization and immobilization were useful indices of soil N availability in response to forest types and management practices. The sampling size for soil δ¹³C was much smaller than the other soil chemical and biological properties due to the different patterns of spatial variation in these soil properties.     

Modelling changes in soil organic matter after planting fast-growing Pinus radiata on Mediterranean agricultural soils

The Kyoto Protocol explicitly allows the storage of carbon (C) in ecosystems resulting from afforestation to be offset against a nation's carbon emissions and paves the way for carbon storage in soils to be eligible as carbon offsets in the future. More information is required about how afforestation affects carbon storage, especially in the soil. We report a study in which soil carbon storage in first‐rotation Mediterranean Pinus radiata plantations, established on former cereal fields and vineyards, was measured and modelled. Measurements were made on plantations of several ages, as well as repeat measurements at the same site after 5 years. We tested the ability of two widely used soil organic matter models (RothC and Century) to predict carbon sequestration in Mediterranean forest soils. Increases in the top 5 cm of soil of about 10 g C m^?2 year^?1 were observed after afforestation of former vineyards, but nitrogen (N) either remained the same or decreased slightly. During afforestation, most organic matter accumulated in the ectorganic layers at a rate of 19 g C m^?2 year^?1 in former vineyards and 41 g C m^?2 year^?1 in former cereal fields. The RothC and Century models were sensitive to previous land use and estimated a carbon sequestration potential over 20 years of 950 and 700 g C m^?2, respectively. The accurate simulation of the dynamics of soil organic matter by RothC, together with measured above‐ground inputs, allowed us to calculate below‐ground inputs during afforestation. The Century model simulated total C and N, including the ectorganic horizons, well. Simulations showed a depletion of N in the below‐ground fractions during afforestation, with N limitation in the former vineyard but not on former cereal land. The approach demonstrates the potential of models to enhance our understanding of the processes leading to carbon sequestration in soils.     

The microbial biomass and activity in soil with shrub (Caragana korshinskii K.) plantation in the semi-arid loess plateau in China

Caragana korshinskii K. is a shrub species which is adapted to arid and semi-arid environments and plays an important role in soil protection. The objective of this study was to determine the influence of this shrub plantation on the soil ecosystem functions driven by microorganisms in the long-term. The changes in the size and activity of soil microbial biomass and the relationship between soil microbial biomass and chemical properties were investigated under shrub plantations aged 6, 18 and 26 years. The results showed that the pH value in the soil decreased gradually, while soil organic carbon (OC) and total nitrogen (TN) significantly increased with the age of C. korshinskii. Although microbial biomass carbon (MBC) and MBC/OC ratio gradually increased, the ratio of basal respiration to MBC (qCO₂) decreased with the age of C. korshinskii. The microbial biomass nitrogen (MBN) and MBC had a positive relationship with soil TN and OC, respectively. The flux of CO₂ decreased with the age of C. korshinskii which had a significant negative relationship with soil OC, TN, MBN and MBC. The results indicate that C. korshinskii plantations may help to improve microbially driven ecosystem functioning through long-term creation of resource-island.     

Dynamics and stabilization of soil organic carbon after nineteen years of afforestation in valley‐type savannah in southwest China

Serious concerns about carbon (C) sequestration capacity and the stabilization of sequestered C in forested soils have emerged in the context of global climate change. The organic C in soil and in soil fractions at four sampling times in Acacia auriculiformis plantations afforested in 1991 were investigated with a combination of density fractionation and acid hydrolysis techniques. The results showed that the accumulation of C in the forested soils had accelerated because the afforestation of wasteland with A. auriculiformis. The C accumulation rates of the surface and subsurface soils averaged 0.38 and 0.17 t C/ha/yr, respectively, during the 19 yr following the afforestation. The percentage of organic C in heavy fraction relative to total soil organic C at the surface soil was 71% in 2003. This value was significantly (P < 0.05) higher than that in 2010 (68%). The chemical recalcitrant C index of light fraction was significantly (P < 0.05) higher than that of heavy fraction in 2003 regardless of soil depth, but both decreased with time. ca. 58–68% of the newly sequestered C was protected by physical mechanism, and 41–50% was transferred into the acid nonhydrolysable fraction during the 12–19 yr after the trees were planted. The chemical stability of the physically protected C remained lower than that of the unprotected C following the afforestation in the valley‐type savannah. However, both the stability values showed a decline with time.     

Microbial biomass dynamics during the decomposition of leaf litter of poplar and eucalyptus in a sandy loam

Soil microbiological properties during decomposition of leaf litter of poplar (Populus deltoides) and eucalyptus (Eucalyptus tereticornis) were studied under laboratory conditions. Microbial biomass C and ninhydrin-N were measured at different intervals up to 90 days following incorporation of poplar and eucalyptus leaves separately @ 20 and 100t ha^-1. In general, the net increase in total biomass C or ninhydrin N following amendment was larger in the soils which received poplar leaves than in the soils that received eucalyptus leaves. The amounts of biomass C, at day 90, in the soils which received eucalyptus leaves @ 20 and 100 t ha^-1 was about half and one-third, respectively, that of the soils that received poplar leaves at the same rates. Similarly, the field soils naturally receiving eucalyptus leaf litter contained about half the amounts of biomass C or ninhydrin N of the soils that received poplar leaf litter. In contrast, the amounts of organic C and total N were more in soils which received eucalyptus leaves both in the laboratory experiment and under field conditions than in the soils that received poplar leaves, indicating that the decomposition of eucalyptus leaves in soils was slower than that of poplar leaves. The ratio of biomass C/soil organic C in soils receiving eucalyptus leaves was about 2–4 times lower than those in soils with no admendment or soils receiving poplar leaves. These results, therefore, suggest that the allelochemicals released into soil during decomposition of eucalyptus leaves had a toxic effect on soil microorganisms and may thus affect the nutrient cycling and hence soil fertility.     

On farm assessment of tillage impact on soil carbon and associated soil quality parameters

No-tillage (NT) farming offers innumerable benefits to soil and water conservation, however, its potential to sequester soil organic carbon (SOC) and related soil properties varies widely. Thus, the impact of long-term (>4 yr) NT-based cropping systems on SOC sequestration and selected soil physical and chemical parameters were assessed across soils within five Major land Resource Areas (MLRAs: 99 and 111 in Michigan; 124 and 139 in Ohio; and 127 in Pennsylvania) in eastern U.S.A. Soil samples were collected from paired fields of NT and plow tillage (PT) based cropping systems and an adjacent woodlot (WL). The SOC concentration, bulk density (ρ_b), texture, pH, electrical conductivity (EC), soil N, coarse particulate organic matter (CPOM) C and N, and nitrate N (NO₃-N) concentrations were determined. Conversion from NT to PT practice increased surface soil pH from 5.97, 6.56 and 6.02 to 6.62, 6.91 and 7.09 under MLRAs 127, 111 and 99, respectively. NT soils had higher SOC concentration soils by 30, 50 and 67% over PT soils at 0–5 cm depth under MLRAs 99, 111 and 127, respectively. Considering the whole soil profile SOC, WL had higher SOC pool than NT and PT practices under MLRAs 99, 111 and 124, however, there was no significant difference (P < 0.05) between NT and PT practices across five soils. Almost the same trend was observed in the case of depthwise soil N content. NT soil had higher N content than PT soils by 27, 44 and 54% under MLRAs 99, 127 and 111, respectively. However, whole soil profile N content of NT soil was significantly higher by 12% than PT soil under MLRA 99. Concentrations of CPOM associated C and N of NT soil was higher than PT soil under MLRAs 99, 111 and 127 at 0–5 soil depth. These results indicated that impact of tillage on soil C and associated soil quality parameters is confined within specific soil types.     

华北平原典型农田土壤微生物生物量碳氮磷库的县域分布特征——以河北省曲周县为例

土壤微生物生物量是土壤中的活性养分库,直接参与土壤碳氮磷硫等元素的形态转化与生物地球化学循环过程,是反映土壤肥力与质量的重要生物指标。基于网格法采样,运用地统计学方法分析华北平原典型农田土壤微生物生物量碳氮磷库的空间分布特征及影响因子。结果表明:河北省曲周县域农田耕层(0~30 cm)土壤微生物生物量库在空间上呈斑块状分布,具有中等变异强度和明显的空间自相关性,微生物生物量碳(MBC)、微生物生物量氮(MBN)、微生物生物量磷(MBP)库储量分别为(C)64.14×10³t、(N)24.55×10³t、(P)2.80×10³t,作物产量与MBC和MBN存在显著正相关关系。不同种植体系下单位质量土壤MBC、MBN、MBP的量存在显著差异,小麦/玉米轮作体系下单位质量土壤微生物生物量的平均量高于棉花连作。土壤微生物生物量库的大小和空间分布均受种植体系和土壤肥力的影响,其中土壤有机碳含量是影响土壤微生物生物量库容及空间分布的一个主要因子。研究结果表明土壤微生物生物量库是我国北方典型农田土壤中不可忽视的潜在有效养分库。     

A modelling analysis of the potential for soil carbon sequestration under short rotation coppice willow bioenergy plantations

Abstract. Rising atmospheric CO₂ concentrations and their association with global climate change have led to several major international initiatives to reduce net CO₂ emissions, including the promotion of bioenergy crops such as short rotation coppice (SRC) willow. Although the above-ground harvested bio-fuel is likely to be the major contributor to the CO₂ mitigation potential of bioenergy crops, additional carbon may be sequestered through crop inputs into plantation soils.   Here, we describe a process-based model specifically designed to evaluate the potential for soil carbon sequestration in SRC willow plantations in the UK. According to the model predictions, we conclude that the potential for soil carbon sequestration in these plantations is comparable to, or even greater than, that of naturally regenerating woodland. Our preliminary, site-specific model output suggests that soil carbon sequestration may constitute about 5% of the overall carbon mitigation benefit arising from SRC plantations. Sensitivity analyses identified the following factors as the principal controls on rates and amounts of soil carbon sequestration under SRC: carbon inputs (net primary production), decomposition rates of the major soil carbon pools, initial soil carbon content (an inverse relationship with rates of soil carbon sequestration), crop/plantation management, and depth of soil being influenced by the bioenergy crop. Our results suggest that carbon sequestration potential is greatest in soils whose carbon content has been depleted to relatively low levels due to agricultural land use practices such as annual deep ploughing of agricultural soils.     

Seasonal and long-term resource-related variations in soil microbial communities in wheat-based rotations of the Canadian prairie

Like all other living organisms, microorganisms depend on nutrients, carbon and energy. Since microorganisms are central to most soil processes, the sustainable management of agricultural soils may need to consider the impact of soil fertility management on the soil microbial community. We tested the hypothesis that different rates of N and P fertilizers, and cropping frequency (modifying C input to soil) influence the size, structure and physiological condition of soil microbial populations residing in the plough layer (top 7.5 cm). For this study, we used a 37-yr old long-term wheat-based rotation experiment located in the semiarid Brown soil zone of Saskatchewan. The experiment included (1) four input treatments: (i) no N or (ii) no P fertilizer application to wheat (Triticum aestivum L.) grown in fallow-wheat-wheat (F-W-W) rotations, and (iii) recommended rates of both N and P fertilizer applied to fallow-wheat (F-W) and (iv) to F-W-W; (2) two rotation phases: fallow and wheat-after-fallow; and (3) four sampling times: 8 June, 4 July, 5 August and 16 September 2003. Increased partitioning into storage lipids of the arbuscular mycorrhizal fungi (AMF) fatty acid methyl ester (FAME) biomarker 16:1ω5 (P=0.04), suggested the accumulation of storage material under low soil N availability. Discriminant analysis detected modifications in soil microbial community structure due to cropping frequency (P=0.001) and sampling time, the effect of which was different in the fallow (P<0.0001) and wheat-after-fallow (P<0.0001) phases of the rotations. Correlation analysis of soil variables conducted in plots growing wheat revealed a dual effect of plants, which stimulated active soil microbial biomass (SMB), possibly through the release of soluble extractable C (C_sol−ext) in soil and, at the same time, SMB competed with wheat for soil water and N. The 37 y of different nutrient input treatments had no effect upon the active soil microbial biomass according to PLFA measurements, despite changes in soil resource-related variables (soil water potential, soil PO₄-P and NO₃-N fluxes, and C_sol−ext concentrations) (P?0.003). The biomass of each of three microbial populations monitored was lowest on 4 July, when the amounts of the soil resources monitored were average, and greatest on 5 August, when N, P and soil moisture availability was lowest. The temporal effect on the biomass of microbial populations seemed unrelated to variation in nutrient or water availability. We conclude that the soil microbial community is adaptable to a wide range of soil conditions. We propose therefore that the occurrence of sudden and dramatic events, such as a heavy rainfall on a dry soil, is the most important determinant of seasonal variation in active soil microbial biomass.     

Organic Matter Dynamics in Reclaimed Lignite Mine Soils under Robinia pseudoacacia L. Plantations of Different Ages in Germany

In temperate regions, cultivation of Robinia pseudoacacia L. has recently received considerable attention because it is a fast-growing species for biomass and bioenergy production, while acting as a potential carbon (C) sink to counterbalance carbon dioxide (CO₂) emissions and an alternative to agricultural crops on marginal sites. The objective of our work was to compare total organic carbon (TOC), total nitrogen (TN), and organic C fractions in postlignite mining soils under different development stages of R. pseudoacacia. Soil samples from three different depths (0–3, 3–10 and 10–30 cm) were taken in plantations 2, 3, 4, and 14 years old (R2, R3, R4, and R14, respectively). The TOC and TN contents increased with increasing tree age in all layers (P < 0.01). In the top 30 cm, TOC and TN stocks ranged from 11.7 to 59.8 Mg C ha^?1 and from 0.30 to 2.61 Mg N ha^?1 at R2 and R14, respectively. The rate of C sequestration was calculated to be 4.0 Mg C ha^?1 year^?1. Microbial biomass C and N were strongly correlated to TOC (r² = 0.96 – 0.81; P < 0.001) and TN contents (r² = 0.92 – 0.91; P < 0.001). The light fraction C (C_LF) accounted for 15–30% and the heavy fraction C for 70% of TOC in all layers. In the 0- to 3-cm layer, C_LF increased by 0.5 g kg^?1 year^?1. The results indicate that plantations of R. pseudoacacia are an attractive alternative to increase soil C contents in reclaimed lignite mining soils. In the short term, microbial biomass C and light fraction C are sensitive and provide an appropriate measure to assess soil C changes caused by cultivation of R. pseudoacacia.     

Phosphorus in the soil microbial biomass

Phosphorus in the soil microbial biomass (biomass P) and soil biomass carbon (biomass C) were linearly related in 15 soils (8 grassland, 6 arable, 1 deciduous woodland), with a mean P concentration of 3.3% in the soil biomass. The regression accounted for 82% of the variance in the data. The relationship was less close than that previously measured between soil biomass C and soil ATP content and indicates that biomass P measurements can only provide a rough estimate of biomass C content. Neither P concentration in the soil biomass, nor the amount of biomass P in soil, were correlated with soil NaHCO₃-extractable inorganic, organic or total P.The calculated mean annual flux of P through the biomass (in a soil depth of 10 cm) in 8 grassland soils was large, 23 kg P ha^?1 yr^?1, and more than three times the mean annual P flux through 6 arable soils (7 kg P ha^?1 yr^?1), suggesting that biomass P could make a significant contribution to plant P nutrition in grassland.About 3% of the total soil organic P in the arable soils was in microbial biomass and from 5 to 24% in the grassland soils. The decline in biomass P when an old grassland soil was put into an arable rotation for about 20 yr was sufficient to account for about 50% of the decline in total soil organic P during this period. When an old arable soil reverted to woodland, soil organic P doubled in 100 yr; biomass P increased 11-fold during the same period.     

Calibration model of microbial biomass carbon and nitrogen concentrations in soils using ultraviolet absorbance and soil organic matter

There is a need for a rapid, simple and reliable method of determining soil microbial biomass (SMB) for all soils because traditional methods are laborious. Earlier studies have reported that SMB‐C and ‐N concentrations in grassland and arable soils can be estimated by measurement of UV absorbance in soil extracts. However, these previous studies focused on soils with small soil organic matter (SOM) contents, and there was no consideration of SOM content as a covariate to improve the estimation. In this study, using tropical and temperate forest soils with a wide range of total C (5–204 mg C g^?1 soil) and N (1–12 mg N g^?1 soil) contents and pH values (4.1–5.9), it was found that increase in UV absorbance of soil extracts at 280 nm (UV₂₈₀) after fumigation could account for 92–96% of the variance in estimates of the SMB‐C and ‐N concentrations measured by chloroform fumigation and extraction (P < 0.001). The data were combined with those of earlier workers to calibrate UV‐based regression models for all the soils, by taking into account their varying SOM content. The validation analysis of the calibration models indicated that the SMB‐C and ‐N concentrations in the 0–5 cm forest soils simulated by using the increase in UV₂₈₀ and SOM could account for 86–93% of the variance in concentrations determined by chloroform fumigation and extraction (P < 0.001). The slope values of linear regression equations between measured and simulated values were 0.94 ± 0.03 and 0.94 ± 0.04, respectively, for the SMB‐C and ‐N. However, simulation using the regression equations obtained by using only the data for forest profile soils gave less good agreement with measured values. Hence, the calibration models obtained by using the increase in UV₂₈₀ and SOM can give a rapid, simple and reliable method of determining SMB for all soils.     

Potential for soil carbon sequestration of eroded areas in subtropical China

Soil plays an important role in the global carbon cycle, and carbon sequestration in soil is important for mitigating global climate change. Historically, soil erosion led to great reductions of soil organic carbon (SOC) storage in China. Fortunately, with the economic development and remarkably effective soil erosion control measures in subtropical China over the past 20 years, soil erosion has been greatly decreased. As a result, soil organic carbon sequestration has gradually increased due to the rapid recovery of vegetation in the area. However, little information exists concerning the potential of soil carbon sequestration in the area. This paper introduces a case study in Xingguo County, Jiangxi Province, China, which used to be a typical area with significant soil loss in subtropical China. This work represents a systematic investigation of the interrelations of carbon sequestration potential with soil erosion types, altitudes, soil types and soil parent materials. In this study, 284 soil samples were collected from 151 sampling sites (51 are soil profile sites) to determine soil physicochemical properties including organic carbon content. Soil organic carbon distribution maps of the surface layer (0–20 cm) and whole profile (0–100 cm) were compiled by linking soil types to the polygons of digital soil maps using GIS. Assuming that SOC was lost following the destruction of native vegetation, these lands hold great promise for potentially sequestering carbon again. The potential of soil carbon sequestration in the study area was estimated by subtracting the organic carbon status in eroded soils from that in non-eroded soils under undisturbed forest. Results show that the potential of SOC in the surface layer is 4.47 Tg C while that in the whole profile is 12.3 Tg C for the entire county. The greatest potential for carbon sequestration (3.72 Tg C) is found in severely eroded soil, while non-eroded soil has the smallest potential. Also, soil carbon sequestration potential decreases with increasing altitude. Soils at altitudes of <300 m show the greatest potential (5.01 Tg C), while those of >800 m have the smallest potential (0.25 Tg C). Among various soil types, red earths (Humic Acrisols) have the greatest potential of carbon sequestration (5.32 Tg C), and yellow earths (Ferralic Cambisols) have the smallest (0.15 Tg C). As for soils derived from various parent materials, soils derived from phyllite possess the greatest carbon sequestration potential, and soils from Quaternary red clays have the smallest.     

Carbon and nitrogen in a Ferralsol under zero‐tillage rotations based on cover,cash or hay crops

To identify crop rotation systems capable of sequestering C and N to 1 metre depth in a subtropical Ferralsol of Southern Brazil managed under long‐term zero‐tillage (21 yrs), we evaluated six crop sequences: wheat (Triticum aestivum)–soybean (Glycine max) [W‐S], the baseline; oat (Avena strigosa, as cover crop)–maize (Zea mays)–wheat–soybean [O‐M‐W‐S]; vetch (Vicia villosa, as legume cover crop)–maize–wheat–soybean [V‐M‐W‐S]; vetch–maize–oat–soybean–wheat–soybean [V‐M‐O‐S‐W‐S]; ryegrass (Lolium multiflorum, for hay)–maize–ryegrass–soybean [R‐M‐R‐S]; and alfalfa (Medicago sativa, for hay)–maize [A‐M]. Compared to W‐S and to 1 metre, the hay‐based system of A‐M showed the largest C and N sequestration rates (0.50 and 0.06 Mg/ha/yr, respectively). Alfalfa, being a perennial legume under cut‐regrowth cycles, possibly added more C and N through roots. The other hay system, R‐M‐R‐S, also sequestered C efficiently (0.27 Mg/ha/yr), but not N (0.01 Mg/ha/yr). The legume‐based system of V‐M‐W‐S sequestered significant amounts of both C (0.29 Mg/ha/yr) and N (0.04 Mg/ha/yr). The grass‐based system of O‐M‐W‐S showed the lowest sequestration of C (0.09 Mg/ha/yr). In all systems, a positive relationship (R² = 0.71) occurred between estimated addition of root C and soil C stock to 1 metre. Whenever C and N sequestration occurred, more than half of that occurred below 20 cm depth. Results suggest that adoption of legume‐based systems, perennially as A‐M or annually as V‐M‐W‐S, is efficient for C and N sequestration in subtropical zero‐tillage soils and that roots possibly contribute more to that sequestration than aboveground biomass.     

Microbial immobilisation and turnover of N labelled substrates in two arable soils under field and laboratory conditions

Microbial biomass N dynamics were studied under field and laboratory conditions in soils of high yield (HY) and low yield (LY) areas in an agricultural field. The objective of the study was to determine the size and activity of soil microbial biomass in the soils of the different yield areas and to compare these data obtained under field and laboratory conditions. Soils were amended with ¹⁵N labelled mustard (Sinapis alba) residues (both experiments) and labelled nitrate (laboratory only) at 30 μg N g⁻¹ dry soil. Soil microbial biomass (SMB) N, mineral N (N_min) and total N content was monitored both in the field and in the laboratory. N₂O efflux was additionally measured in laboratory treatments. Isotope ratios were determined for SMB in both experiments, for all other parameters only in the laboratory treatments. In the laboratory less amounts of added substrate N were immobilised by the SMB in HY soils compared to LY soils, whereas in the field immobilisation of added N by SMB was higher in HY soils initially and slightly lower after 40 days of incubation. Calculated turnover times in the laboratory nitrate, laboratory mustard and field mustard amendments were 0.18, 0.27 and 0.74 years (HY) and 0.22, 0.61 and 1.01 years (LY), respectively. The turnover times of added substrate N always showed the trend to be faster in HY soils compared to LY soils. A faster turnover of nutrients in the HY soils may involve a better nutrient supply of the plants, which coincides with the higher agricultural yield observed in these areas.