以反刍时间为指标测定放牧家畜采食量方法的探讨

<正> 绪言作为测定放牧家畜采食量的一般方法可以列举出收割法,体重差法、不消化指示物质法等,此外,虽然还提出食道瘘管(Fistula)法和第一胃(rumen)瘘管法等可是测定值的精确程度和测定操作复杂程度等存在问题仍然是很多的。关于家畜的放牧活动,已经进行了许多研究,特别是了解到放牧时的采食行为,以及反刍行为,与采食量是有某些关系。然而,采食和反刍行为受着草地的植被,草的质量以及其他多种条件的影响,反刍行为与采食草之间存在的相互关系尚未进行过充分的探讨。     

放牧家畜采食量和择食性测定方法的研究进展

文章就国内外关于放牧家畜采食量和采食种类测定方法的研究现状进行了回顾与总结,分析了影响其测定的主要因素,并提出了合理的建议,为今后开展草地放牧家畜的采食量、择食性及其测定方法的研究提供了科学参考.     

放牧生态系统家畜牧食行为研究进展

家畜牧食行为包括采食、反刍,游走,饮水,卧息和排泄粪尿,其中采食和反刍是两大主要行为。本文介绍了放牧家畜与牧草间的相互关系,放牧通过采食牧草,降低牧草光合叶面积指数而影响牧草生长发育。放牧作为一种人为干预,影响植被组成和群落结构,高放牧压力下,导致草地退化。在放牧情况下,牧草具有避牧和耐牧两种生存策略。由于放牧草地物种多样,家畜选择采食适口性高的植物或植物组成部分。介绍了国内外计算采食量的各种方法,包括计算放牧前后草地牧草生物量,通过内外源标记物计算排粪量或通过采食时间,单口采食率、单口采食量来计算采食量。家畜采食成分的测定一直以来都是难点,有直接观测法、瘤胃内容物或粪便镜检法、瘤胃瘘管法和炼烷烃技术等。     

食道瘘管法在放牧家畜采食习性研究中的运用

本文介绍了目前广泛运用于草地放牧研究中的食道瘘管法的发展、食道瘘管手术及护理、食道瘘管样的制取、食道瘘管样品用于放牧家畜采食的营养成分、植物成分、采食量和消化率方面的测定，以及与其它常用的方法——放牧前后样方测定法、扣笼与未扣笼双样方法和模拟家畜采食采摘法对放牧家畜采食的营养成分、植物成分、采食量和消化率测定的比较，认为代表放牧家畜真实采食日粮的食道疹管样在以上各项指标的测定中都优于其它测定方法所得到的样品，更能代表放牧家畜的实际采食习性。     

放牧家畜采食量的测定

采食是动物-植物相互作用的重要途径,是维持生态系统物质和能量循环的主要过程。动物的生产水平和草地的可持续性取决于动物的采食量,明确放牧家畜采食量是草地管理的关键之一。目前,直接观测法、指示剂法、粪氮指数法和近红外光谱法等现有技术被广泛运用到采食量的测定中。直接观测法利用较低成本对采食量进行计算,但主观性较强;食道瘘管法能够把握采食量却忽略了动物福利;近红外光谱法和饱和链烷烃法在测定采食量的同时能够认识家畜的择食特征。为此,本文对现有常规测定方法进行综述,分析影响测定放牧家畜采食量的主要因素,并提出放牧行为与无人机技术相结合等新思路,为今后草原精准化管理提供技术支撑。     

放牧家畜采食量估测方法研究进展

放牧家畜采食量是评价草地生态系统转化效率和动物生产力的主要参数,受草地植被生长情况、环境条件、放牧方式、家畜生理特征及其瘤胃内环境等因素影响。放牧家畜采食量的准确测定一直以来都是食性研究的难点和热点问题,关系到动物营养状态的评估、补饲策略的制定以及食性理论的探索。传统采食量估测方法大多基于观察法或放牧前后牧草生物量差值法,这些方法对动物的干扰较小,但是受技术本身和适用范围的限制,如未能估测单个动物的采食量,很难适用于大范围的采食量估测。链烷烃技术和近红外技术的出现为准确测定采食量提供了前所未有的机遇,不仅能克服传统测定方法的缺点,还在准确率、适用范围（圈养动物和放牧动物）等方面优势较为明显,但由于粪便回收率和数据库完整性等因素的影响,目前应用于生物多样性较高草地的放牧动物采食量估测尚有难度。在简要总结不同方法的原理和影响因素基础上,综述了差值法、内标法、链烷烃法和近红外法在放牧家畜采食量测定方面取得的最新进展及存在的问题,并建议多种方法结合可以准确测定放牧家畜采食量,以期为制定合理的草地生态系统管理策略和实现草地可持续生态服务功能提供新思路和启发。     

放牧家畜采食量和采食成分测定技术评述

放牧家畜的采食量是人们了解放牧系统动力学的关键之一。研究已查明，了解了放牧家畜的采食量和采食成分就能明晰家畜的营养状况，预测其生产性能，从而为草地管理决策提供目标，为优化资源利用提供基础数据。然而，估测放牧家畜的采食量及其组分是困难的，也是昂贵的。虽然那些改进的技术和方法有效地增强了人们获取家畜牧食行为数据的能力，但是测定放牧动物的采食量、采食成分和养分消化率一直以来都是营养学研究的挑战，方法众多，却各有利弊。因此，本研究针对目前估测放牧家畜采食量和采食成分的常用方法，如模拟采食法、牧前牧后差额法、酸不溶灰分法、三结合法、植物蜡层指示剂法和近红外光谱技术法，对它们的利弊和准确性进行讨论，并探析未来发展趋向，为今后的研究提供资料。     

放牧试验中估算哺乳奶牛牧草采食量的两种方法的比较

<正> 放牧奶牛的采食置可以用测定牧草('t Mannerje,1978)或测定家畜来进行估算(Ragmond,1969;Corbett,1978)。在草地采样技术中,根据放牧前和放牧后取样的差异法,对提供有效的牧草采食量测定,具有最大的潜力;但只有当放牧期较短和家畜密度相当高时才能体现出来(Pigden和 Miason,1969)。用未消化产物的比率法测定家畜估算采食量(Ragmond,1969;     

放牧家畜采食量测定方法的研究

本文阐述了国内外关于放牧家畜采食量测定方法的研究概况，并对10种采食量的测定方法进行了综述，同时讨论了采食量测定误差的影响因素，提出合理的建议，这对今后研究放牧家畜采食量及其测定方法具有借鉴作用。     

肉羊随意采食量预测模型研究进展

文中阐述了国内外关于放牧家畜采食量测定方法的研究概况,并对10多种采食量的测定方法进行了综述,同时讨论了采食量测定误差的影响因素,提出合理的建议,文中较系统地总结了饱和链烷烃技术在评价放牧家畜食性、食量的研究进展,并对其应用前景进行了展望。     

白术、微米白术和白术多糖对断奶仔猪生长性能和肠道形态及微生态区系的影响

试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。     

Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia

Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.     

Phase I evaluation of CCNU (lomustine) in tumor-bearing cats

1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.     

Internal Parasites and Haematological Values in Cattle Slaughtered in Buea Subdivision of Cameroon

Tropical Animal Health and Production -     

Chemotaxis of macrophage in inflammation

Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.     

Prevalence of gastric lesions in racing Alaskan sled dogs

Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.     

Y‐chromosomal variation of local goat breeds of Turkey close to the domestication centre

Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.     

Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects

Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.     

Changes in proportion of canine urinary calculi composed of calcium oxalate or struvite in specimens analyzed from 1981 through 2001

The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.     

The Epidemiology of Gastrointestinal Nematodes of Dairy Cattle in Central Kenya

The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.