Imbalanced dietary methionine-to-sulfur amino acid ratio can affect amino acid profiles,antioxidant capacity,and intestinal morphology of piglets

Animal protein sources such as fishmeal and plasma powder are excellent and indispensable sources of energy, amino acids, and minerals in animal production. Amino acid imbalance, especially methionine-to-sulfur amino acid (Met:SAA) ratio, caused by an imbalance of animal protein meal leads to growth restriction. This study was conducted to evaluate the effects of imbalanced Met:SAA ratio supplementation of different animal protein source diets on growth performance, plasma amino acid profiles, antioxidant capacity and intestinal morphology in a piglet model. Twenty-four weaned piglets (castrated males; BW = 10.46 ± 0.34 kg), assigned randomly into 3 groups (8 piglets/group), were fed for 28 d. Three experimental diets of equal energy and crude protein levels were as follows: 1) a corn-soybean basal diet with a Met:SAA ratio at 0.51 (BD); 2) a plasma powder diet with a low Met:SAA ratio at 0.41 (L-MR); 3) a fishmeal diet with a high Met:SAA ratio at 0.61 (H-MR). Results revealed that compared to BD, L-MR significantly decreased (P < 0.05) the activities of plasma total antioxidant capacity and glutathione peroxidase, plasma amino acid profiles, and significantly reduced (P < 0.05) villus height and crypt depth in the duodenum and jejunum. Additionally, L-MR significantly reduced (P < 0.05) the mRNA expression level of solute carrier family 7 member 9 (SlC7A9) in the ileum, and significantly increased (P < 0.05) mRNA expression levels of zonula occludens-1 (ZO-1) in the duodenum, and Claudin-1, ZO-1, sodium-coupled neutral amino acid transporters 2 (SNAT2) and SlC7A7 in the jejunum. H-MR significantly increased (P < 0.05) plasma SAA levels, and significantly reduced (P < 0.05) average daily feed intake, villus height, and villus height-to-crypt depth (VH:CD) ratio in the ileum compared to BD. In conclusion, L-MR may result in oxidative stress and villous atrophy but proves beneficial in improving intestinal barrier function and the activity of amino acid transporters for compensatory growth. H-MR may impair intestinal growth and development for weaned piglets. The research provides a guidance on the adequate Met:SAA ratio (0.51) supplementation in diet structure for weaned piglets.     

Maternal butyrate supplementation affects the lipid metabolism and fatty acid composition in the skeletal muscle of offspring piglets

Maternal sodium butyrate (SB) intake has important effects on offspring growth and development. This study aimed to investigate the impacts of maternal SB supplementation during gestation and lactation on fatty acid composition and lipid metabolism in the offspring skeletal muscle of pigs. Twenty sows (Yorkshire, parity 2 to 3) were assigned to the control group (diets without SB, n = 10) and SB group (diets with 0.1% SB, n = 10). The results showed maternal SB supplementation throughout gestation and lactation increased (P < 0.05) body weight of offspring piglets at weaning. The concentrations of triglyceride in plasma and milk were enhanced (P < 0.05). Maternal SB induced (P < 0.05) lipid accumulation with increased expression of peroxisome proliferator activated receptor γ (PPARγ) by enrichment of the acetylation of H3 acetylation K27 (H3K27) in offspring skeletal muscle. Meanwhile, the concentrations of C18:2n-6, C18:3n-3, total polyunsaturated fatty acid (PUFA), n-6 PUFA and n-3 PUFA decreased (P < 0.05) in skeletal muscle of weaning piglets derived from SB sows. Together, these results showed that maternal SB supplementation could influence offspring growth performance, lipid metabolism and fatty acid composition of the skeletal muscle.     

Feed restriction followed by realimentation in prepubescent Zebu females

In the present study, the effect of restricting the feed intake for 77 days and subsequent compensatory growth for 50 days of Sindi females were evaluated. Eighteen animals with an initial age of 21 months and a mean weight of 211.7 kg were placed into three groups according to the following alimentary regimens: feed ad libitum, feed restricted to 20 % dry matter, and feed restricted to 40 % dry matter. In the feed-restriction phase, the nutrient intake decreased (P?<?0.001) with an increase in the restriction level. As a consequence, the observed decrease in ingestion and serum concentrations of total protein, albumin and globulin, urea, glucose, calcium, and phosphorus were inversely proportional (P?<?0.001) to the restriction level. Significant differences in the nutrient intake and serum concentration were not observed in the realimentation phase (P?<?0.05). When animals in the control group reached the end of the feed-restriction phase, their weights (P?<?0.05) were similar to those in the 20 % restricted group, and both obtained a final weight that was greater than that of animals in the 40 % restricted group. In the feed-restriction phase, the control group had a similar mean daily weight gain (P?>?0.05) to animals in the 20 % restricted group and (P?<?0.05) 40 % restricted group. However, in the realimentation phase, the 40 % restricted group obtained greater weight gain rates (P?<?0.05), better food conversions, and partial compensatory gains. In particular, none of the restricted groups reached the final weight of the control group. In the feed-restriction phase, ingested nitrogen, nitrogen excreted in urine and feces, nitrogen balance and retained nitrogen decreased (P?<?0.05) with an increase in the restriction level. In the realimentation phase, none of the nitrogen balance variables were influenced by the restriction level (P?<?0.05). Females in the 40 % restricted group presented better food conversion rates and greater weight gains in the realimentation phase. Based on the animals’ compensatory weight gain, a feed-restriction rate of 20 and 40 % can be adopted as a nutritional management practice for prepubescent Sindi females.     

Effects of single or trickle Haemonchus contortus experimental infection on digestibility and host responses of naïve Creole kids reared indoor

The objective of this study was to compare the effects of the type of Haemonchus contortus experimental infection (trickle infection, TI versus single infection, SI) on feed intake, nutrients digestibility, parasitological and haematological measures, and plasma leptin in Creole kids. The animals were infected over 2 periods (challenge 1 and challenge 2) of 6 weeks each, corresponding respectively to the primary and the secondary infection. Periods prior infection (1 week each) were considered as controls. The primary infection was realized with 35 Creole kids (18.40 ± 3.76 kg BW) housed in individual boxes and fed a hay-based diet. The secondary infection continued with 29 kids (21.90 ± 3.40 kg BW) from the initial 35. A total of 6 kids and 8 kids were slaughtered for measuring nematode burden at the end of the primary and the secondary infection, respectively. Measurements of nutrients digestibility were made at 0, 3 and 5 weeks post-infection for both challenges. Faecal egg count (FEC), blood eosinophilia and packed cell volume (PCV) were monitored weekly. Feed intake (dry matter intake, DMI) and nutrients digestibility were negatively affected by H. contortus infection only during the primary infection. Plasma leptin changed significantly over time (P = 0.0002) but was not affected by the infection type. Effect of infection type was observed only on crude protein digestibility during the primary infection, which was lower in the TI group (P < 0.01). The overall level of blood eosinophilia was significantly higher in the TI group (P < 0.0001) during both challenges. The overall FEC mean was significantly higher in the SI compared with the TI groups, during both challenges (P < 0.02). These results were related to the mean female length significantly higher in the SI group compared with the TI group during challenge 1 (P = 0.004), and the number of adult nematode significantly lower in the TI group compared with the SI group during the challenge 2 (P = 0.05). The results showed that the response of Creole kids to H. contortus experimental infection was in part dependent on the type of experimental infection. Our data suggest that plasma leptin would not be involved in the response of Creole kids against H. contortus infection, as no relationship between its plasma level and the transient reduction in voluntary feed intake observed in both groups during the primary infection was observed.     

Shade effects on feeding behavior,feed intake,and daily gain of weight in female goat kids

Solar radiation and high ambient temperatures negatively affect feeding time, performance, and animal welfare in several species. The provision of shade is a simple method that helps to minimize the negative effects. To determine whether shade influences feeding behavior, feed intake, and daily weight gain (DWG) in female goat kids, 40 dairy goat kids were used in 2 similar pens whose feeders were shaded (n = 20) or unshaded (n = 20) during 60 days. From May to July, behavioral data were collected through 10-minute scan samples during a 24-hour period for a total of 300 hours. Both pens were shaded on the opposite side to the feeder with 15 m² each in a resting area. All goat kids were observed for their position inside the pen, and the number of times they were seen eating was recorded. When the concentrate was provided (between 13:00 and 13:30 hours, as usually in the farm), the time was recorded until >50% of the animals stopped feeding and went back to the resting area (concentrate test). Food wastes were collected and weighed daily to calculate the food consumption. Ambient and black globe temperatures were daily recorded. Body weight was recorded every week to calculate DWG. A higher percentage of animals feeding was recorded in the shaded feeders than that in the unshaded feeders (P < 0.05). Food refusal was higher in the unshaded feeders (30 ± 1.8%) than that in the shaded feeders (25 ± 1.9%; P = 0.05). The concentrate test duration was 26.6 minutes (±1.3) in the shaded feeders and 16.1 minutes (±1) in the unshaded feeders (P < 0.05). The concentrate test duration was negatively correlated to the ambient temperature in the unshaded animals (r = ?0.50 and r² = 0.25; P = 0.02), and it was not significant in the shaded ones (r = ?0.23, r² = 0.05; P > 0.05). DWG was not different between groups (0.08 ± 0.03 kg per group; P > 0.05). Results suggest that shade on feeders helps to ameliorate some negative effects of solar radiation increasing feeding time and feed intake in female goat kids. This could be of great interest to prevent performance and welfare negative affectations.     

Effect of doe milking and supplementation using <Emphasis Type="Italic">Dichrostachys cinerea</Emphasis> pods on kid and doe performance in grazing goats during the dry season

Effects of supplementation and milking of Matebele does four weeks pre-partum to weaning, on milk yield, kid birth weight and growth rate were studied. Forty does were divided into four treatments: supplemented and milked (SM), supplemented and not milked (SNM), not supplemented and milked (NSM); and not supplemented and not milked (NSNM). Supplemented goats were given 200 g/doe/day of Dichrostachys cinerea pods containing 19% CP. The study was carried out in September during time of feed unavailability and animals were grazed on rangelands for seven hours before being given the supplement. Fifty-six kids were born from all the experimental animals and 24 were singles whereas 32 were twins. Milk yield was higher (P < 0.05) in supplemented than non-supplemented does. Although supplementation had no effect on kid birth weights, growth rates were higher (P < 0.05) in the SNM group (103 ± 10.1 g/day) than in the SM group (74 ± 10.8 g/day). Among the non-supplemented groups, kids from milked does had higher (P < 0.05) daily gains (85 ± 10.9 g/day) than those from non-milked does (67 ± 10.7 g/day). Weaning weights were higher (P < 0.05) in SNM does (12.8 ± 1.30 g/day) than in the SM does (10.2 ± 1.17 g/day). Among kids from the non-supplemented does, those from milked does had superior (P < 0.05) weaning weights (11.2 ± 1.23 g/day) than those from non - milked does (9.2 ± 1.27 g/day). Mortalities were also lowest in kids born from SNM does. It is concluded that pre-partum to weaning supplementation generally increases milk production which then increases kids growth rates and weaning weights, as well as reduce kid mortalities.     

Supplementing daidzein in diets improves the reproductive performance,endocrine hormones and antioxidant capacity of multiparous sows

Certain hormones play important roles in modulating mammalian reproductive behaviour. Daidzein is a well-known isoflavonic phytoestrogen that possesses oestrogenic activity. This study was conducted to probe the effects of daidzein supplementation in gestation diets on the reproductive performance in sows. A total of 120 multiparous sows (Landrace × Yorkshire) were randomly assigned to 2 groups (n = 60) and fed either a base diet (control) or one containing 200 mg/kg daidzein during gestation. We discovered that daidzein supplementation significantly increased the total number of piglets born per litter and number of piglets born alive per litter (P < 0.05), decreased the farrowing time (P < 0.05) and increased the serum oestrogen and progesterone concentrations (P < 0.05) at 35 d of gestation. Moreover, serum immunoglobulin G (IgG) concentration and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were higher in the daidzein-treated group than in the control group at 35 d of gestation (P < 0.05). Daidzein increased the serum SOD activity and total anti-oxidative capacity (T-AOC) at 85 d of gestation (P < 0.05). Interestingly, daidzein elevated the expression levels of the sodium-coupled neutral amino acid transporter 1 (SLC38A1) and insulin-like growth factor 1 (IGF-1) genes in the placenta (P < 0.05). These results suggest that daidzein ingestion could improve sow reproductive performance by changing serum hormones, elevating anti-oxidative capacity and up-regulating critical functional genes in the placenta.     

Tannic acid extracted from gallnut prevents post-weaning diarrhea and improves intestinal health of weaned piglets

This experiment was conducted to evaluate the effects of different levels of tannic acid (TA) on growth performance, diarrhea rate, nutrient digestibility and intestinal health in weaned piglets. A total of 180 weaned piglets (Duroc × Landrace × Yorkshire, 24 d of age, initial average BW = 7.77 ± 0.17 kg) were allotted to 5 groups (6 pigs/pen and 6 replicates/group) in a randomized complete block design according to their gender and body weight. Piglets were fed a basal diet, or the basal diet supplemented with 0.05%, 0.1%, 0.2% or 0.4% TA for 28 d. The supplementary levels of TA in the diets were obtained by adding tannalbin containing 51% TA and 40.17% protein. The results showed that, compared with the CON group, dietary TA did not affect ADFI, ADG or F:G, and linearly reduced (P < 0.01) the diarrhea rate and diarrhea index of piglets. There were no significant effects on apparent total tract digestibility (ATTD) in the 0.05%, 0.1% and 0.2% TA groups, while negative effects (P < 0.05) on apparent digestibility of crude protein and gross energy were observed in the 0.4% TA group. In addition, the nutrient digestibility of dry matter, crude protein and gross energy linearly decreased (P < 0.01) with the increase of TA dosage. Supplementation of TA increased (P < 0.05) the villus height of the duodenum and jejunum, as well as increased (P < 0.05) catalase (CAT) activity in serum. Dietary TA improved (P < 0.05) the Bacillus counts in cecal digesta. Further, TA significantly improved (P < 0.05) Bacillus counts and reduced (P < 0.05) the Escherichia coli counts in colonic digesta. The concentration of acetic acid, propionic acid, butyric acid and isovaleric acid in cecal digesta were significantly increased (P < 0.05). The mRNA expression level of zonula occludens-1 (ZO-1), zonula occludens-2 (ZO-2), and claudin-2 (CLDN-2) in the jejunum were greater (P < 0.05) in TA supplemented groups. The study showed that, compared to the control, TA prevented post-weaning diarrhea and improved intestinal health of weaned piglets, and the appropriate level of TA supplementation would be from 0.1% to 0.2%.     

Probiotic Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 enhance growth performance of broilers by improving the intestinal health

With the ever-growing strict prohibitions on antibiotic growth promoters (AGP) in animal production, in-feed probiotics are becoming attractive alternatives to antibiotics in the poultry industry. To investigate the effects of Paenibacillus polymyxa 10 and Lactobacillus plantarum 16 on the growth performance and intestinal health of broilers, 540 male Cobb 500 broilers of 1 d old were randomly divided into 3 groups with 6 replicates per group and 30 chicks per replicate. Broilers were fed with either a basal diet or basal diets supplemented with 1 × 10⁸ colony-forming units (CFU)/kg P. polymyxa 10 (BSC10) or L. plantarum 16 (Lac16) for 42 d. Results showed that Lac16 treatment improved (P < 0.05) the growth performance (body weight and feed conversion) of broilers at the starter phase, while BSC10 treatment slightly improved (P > 0.05) the growth performance of the starter phase broilers. The increased villus height (P < 0.05) at d 14, 21 and 42 and villus height to crypt depth ratio (P < 0.05) at d 14 and 21 were observed in the ileum of the 2 probiotic groups. Besides, transmission electron microscopy results showed that the 2 probiotics enhanced the intestinal epithelial barrier. Both probiotic treatments up-regulated (P < 0.05) the mRNA expression of fatty acid binding protein 1 (FABP1) and sodium-dependent glucose transporters-1 (SGLT-1) in the ileal mucosa of broilers at d 21. In addition, BSC10 and Lac16 treatments significantly (P < 0.05) increased the relative abundance of short-chain fatty acids-producing bacteria, such as Butyricicoccus pullicaecorum, Faecalibacterium prausnitzii, Lachnospira and Coprococcu, and significantly (P < 0.05) decreased the relative abundance of enteric pathogens (Escherichia coli, Bacteroides fragilis and Shigella sonnei). Furthermore, the 2 probiotic treatments also increased the positive connection among the intestinal microbes and the carbohydrate metabolism-related pathways of the intestinal bacteria (P < 0.05), with decreasing (P < 0.05) nucleotides biosynthesis-related pathways of the intestinal bacteria. Overall, these results suggest that the 2 probiotics, especially Lac16, have a potential beneficial effect on the growth performance and intestinal health of starter phase broilers.     

Effects of dietary lipid-to-carbohydrate ratio on growth and carbohydrate metabolism in juvenile cobia (Rachycentron canadum)

This experiment was conducted to investigate the effects of dietary lipid-to-carbohydrate ratio on growth and carbohydrate metabolism in juvenile cobia (Rachycentron canadum). Six isonitrogenous diets were prepared to vary in lipid-to-carbohydrate ratio (g/g) as follows: D1, 2.26; D2, 1.31; D3, 0.78; D4, 0.47; D5, 0.34; and D6, 0.23. Cobias were fed to satiety for 8 weeks. The weight gain and protein efficiency ratio in D1 group were significantly lower than those in other groups (P < 0.05), accompanied by a lower level of feed conversion ratio (P < 0.05). Protein retention efficiency in D4 and D6 and whole body protein in D4 and D5 were significantly higher than those in D1 group (P < 0.05). Survival rate in D4 group was the highest among all groups and was significantly higher than that in D1, D2 and D5 (P < 0.05). In terms of serum triglyceride, D1 and D2 were significantly higher than D6 (P < 0.05). Hepatosomatic index in D3 and D4 was significantly lower than that in D1 (P < 0.05). Fructose-1,6-diphosphatase in D4 was significantly higher than that in D1 and D3 (P < 0.05). Phosphofructokinase in D3 and D4 and malic enzyme in D4 and D5 were significantly higher than those in other groups (P < 0.05). Results indicate that cobia utilizes carbohydrates as energy source more efficiently than it utilizes lipids. The optimal lipid-to-carbohydrate ratio in juvenile cobia diets is 0.47.     

Hepatic lipid metabolism is affected by a daily 3-meal pattern with varying dietary crude protein with a pig model

The present study was conducted to evaluate the effects of 3 meals administered daily with varying dietary crude protein (CP) contents on hepatic lipid metabolism with a pig model. Pigs were divided into 3 groups according to the following feeding patterns: feeding a basal CP diet 3 times daily (3C); feeding a high CP diet for breakfast, the basal CP diet for lunch, and a low CP diet for dinner (HCL); and feeding the low CP diet for breakfast, the basal CP diet for lunch, and the high protein diet for dinner (LCH). Three groups took equivalent diet per meal ensuring that every pig was fed with similar dietary formulae daily. Results showed that HCL feeding pattern reduced the relative kidney weight (P < 0.05), and LCH feeding pattern increased the relative liver weight of pigs (P < 0.05) when compared with those in the 3C group. Plasma urea nitrogen (P < 0.01) and lipase (P < 0.05) decreased in the HCL group but increased in the LCH group. Both HCL and LCH feeding patterns reduced plasma triglycerides (P < 0.01), non-esterified fatty acids (NEFA) (P < 0.01), and hepatic crude fat (0.05 < P < 0.10) of pigs. Real-time quantitative PCR (RT-qPCR) results showed that dynamic feeding patterns down-regulated (P < 0.05) the mRNA level of lipid metabolism related genes, including adipose triglyceride lipase (ATGL), acetyl-CoA carboxylase (ACCα), liver X receptor (LXRα) in the liver, and negatively regulate elements of circadian clock, including period 1 (Per1), period 2 (Per2), cryptochrome (Cry2), which in turn, upregulated (P < 0.05) the protein expression of positive regulate element brain and muscle Arnt-like 1 (BMAL1) when compared with 3C group. Overall, our findings suggested that dynamic feeding patterns may affect hepatic lipid metabolism via regulation of the circadian clock.     

Dietary rumen-protected L-arginine or N-carbamylglutamate attenuated fetal hepatic inflammation in undernourished ewes suffering from intrauterine growth restriction

This study aimed to explore whether dietary rumen-protected L-arginine (RP-Arg) or N-carbamylglutamate (NCG) supplementation to feed-restricted pregnant ewes counteracts fetal hepatic inflammation and innate immune dysfunction associated with intrauterine growth retardation (IUGR) in ovine fetuses. On d 35 of pregnancy, twin-bearing Hu ewes (n = 32) were randomly assigned to 4 treatment groups (8 ewes and 16 fetuses per group) and fed diets containing 100% of the NRC requirements (CON), 50% of the NRC requirements (RES), RES + RP-Arg (20 g/d) (RESA), or RES + NCG (5 g/d) (RESN). At 08:00 on d 110 of gestation, fetal blood and liver tissue samples were collected. The levels of triglyceride, free fatty acid, cholesterol and β-hydroxybutyrate in the fetal blood of RESA and RESN groups were lower (P < 0.05) than those of the RES group, but were higher (P < 0.05) than those of the CON group. The interleukin (IL)-6 and IL-1 levels in fetal blood and liver tissue as well as the myeloid differentiation primary response 88 (MyD88), transforming growth factor β (TGFβ), and nuclear factor kappa B (NF-κB) mRNA levels in the fetal liver were decreased (P < 0.05) by the NCG or RP-Arg supplementation compared to the RES treatment. Similarly, the toll-like receptor (TLR)-4, MyD88, TGFβ, and p-c-Jun N-terminal kinase (JNK) protein levels in the fetal liver were reduced (P < 0.05) in the NCG and RP-Arg -supplemented groups compared to the RES group. These results showed that dietary supplementation of RP-Arg or NCG to underfed pregnant ewes could protect against IUGR fetal hepatic inflammation via improving lipid metabolism, down-regulating the TLR-4 and the inflammatory JNK and NF-κB signaling pathways, and decreasing cytokine production in ovine fetal blood and liver tissue.     

Meat quality characteristics of Turkish indigenous Hair goat kids reared under traditional extensive production system: effects of slaughter age and gender

Meat quality characteristics of Turkish indigenous Hair goat kids reared under an extensive production system were investigated in this study. A total of 60 Hair goat kids (30 females and 30 males) were slaughtered at 3, 6, 9, and 12 months of ages. Slaughter age significantly affected meat quality characteristics. Cooking loss (P?<?0.01) and shear force value (P?<?0.001) increased with age. Meat color became darker red with increasing slaughter age (P?<?0.001). The percentage of fat of musculus (M.) semimembranosus increased with age (P?<?0.01). The percentage of saturated fatty acids was significantly higher (P?<?0.01), whereas the percentage of unsaturated fatty acids was lower (P?<?0.001) in the M. longissimus dorsi of the kids slaughtered at 3 months of age than those in the other age groups. The females had higher intramuscular fat than that in males (P?<?0.001) and meat from male kids contained a higher percentage of polyunsaturated fatty acid than that from females (P?<?0.01). In conclusion, meat from Hair kids slaughtered between 3 and 9 months of ages had better quality than those slaughtered at the other ages and also meat from male kids had better quality than those female kids slaughtered at the same age under extensive production system.     

Alterations on vitamin C synthesis and transportation and egg deposition induced by dietary vitamin C supplementation in Hy-Line Brown layer model

In ovo feeding of vitamin C (VC) has positive effects on the growth performance, immune and antioxidant function in poultry, which indicates that increasing VC content in eggs may be of benefit. This study was to investigate the effects of dietary VC supplementation on VC synthesis and transportation and egg deposition. In Exp. 1, in order to select a suitable animal model, VC content was detected in different eggs from different layer species. Vitamin C content was lower in ISA Brown breeder eggs and Hy-Line Brown layer eggs (P < 0.05) then in Arbor Acres breeder eggs. In Exp. 2, a total of 24 Hy-Line Brown layers (42-week-old) were randomly divided into 3 treatments with 8 replicates and fed a basal diet with VC at 0, 200 and 400 mg/kg. Sodium-dependent VC transporter 1 and 2 (SVCT1 and SVCT2) expressions were higher in ileum than in duodenum and jejunum (P < 0.05). SVCT1 expression was higher but SVCT2 expression was lower in the magnum than in the ovary (P < 0.05). L-Gulonolactone oxidase (GLO) and SVCT1 expressions were higher but SVCT2 was lower in the kidney than in the liver (P < 0.05). Dietary VC supplementation at 400 mg/kg increased SVCT1 expression in duodenum, ovary and magnum, but decreased GLO and SVCT1 expression in liver (P < 0.05). Dietary VC supplementation at 200 and 400 mg/kg increased SVCT2 expression in duodenum, but decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver (P < 0.05). Dietary VC supplementation promoted VC absorption in duodenum and jejunum, but reduced endogenous VC synthesis in liver and kidney. Although dietary VC supplementation enhanced VC transportation in ovary and magnum, it did not increase VC deposition in produced eggs.     

Assessment of growth performance,immune responses,serum metabolites,and prevalence of leg weakness in broiler chicks submitted to early-age water restriction

The objective of this study was to evaluate the effects of two methods of early-age water restriction on performance, some immune responses, serum metabolites, and prevalence of leg weakness in broiler chicks. One-hundred-eighty-seven-day-old chicks (Ross 308) were allocated to three treatments with four replicates based on a completely randomized design. Treatments included the control group, where birds had free access to water, and water-restricted groups, where access to water was limited to three 2-h periods (WRT1) or a 12-h period (WRT2) per day from days 7 to 17. Chicks in the control group were significantly heavier (P < 0.05) at 17 and 46 days of age compared to the restricted birds. In the WRT2 group, feed intake decreased significantly over the 17- to 28-day period while feed conversion ratio was poorer during water restriction (P < 0.05). Antibody titers against Newcastle and SRBC were higher (P < 0.05) for chicks with ad libitum access to water. Birds in the restricted groups exhibited a higher heterophil to lymphocyte ratio compared to the control group (P < 0.05). The treatments had no significant impact on serum metabolites including protein, triglyceride, total cholesterol, high-density lipoprotein and low-density lipoprotein cholesterols, calcium, and phosphorus concentrations. Walking ability, tibial dyschondroplasia, foot pad, hock burn, and valgus/varus angulation were not significantly influenced by water restriction. In conclusion, the current results indicate that early-age water restriction negatively influenced productive traits and immunological responses of broiler chicks and failed to have any favorable impacts on leg health.     

Zinc alleviates the heat stress of primary cultured hepatocytes of broiler embryos via enhancing the antioxidant ability and attenuating the heat shock responses

Zinc (Zn) has been shown to attenuate the adverse effects of heat stress on broilers, but the mechanisms involving this process remain unclear. We aimed to investigate possible protective mechanisms of Zn on primary cultured hepatocytes of broiler embryos subjected to heat stress. Three experiments were conducted. In Exp. 1, hepatocytes were treated with 0, 50, 100, 200, or 400 μmol/L added Zn as inorganic Zn sulfate (iZn) for 12, 24 or 48 h. In Exp. 2, cells were exposed to 40 °C (a normal temperature [NT]) and 44 °C (a high temperature [HT]) for 1, 2, 4, 6, or 8 h. In Exp. 3, cells were preincubated with 0 or 50 μmol/L Zn as iZn or organic Zn lysine chelate (oZn) for 8 h under NT, and then incubated with the same Zn treatments under NT or HT for 4 or 6 h. The biomarkers of antioxidative status and heat stress in cells were measured. The results in Exp. 1 indicated that 50 μmol/L Zn and 12 h incubation were the optimal conditions for increasing antioxidant ability of hepatocytes. In Exp. 2, the 4 or 6 h incubation under HT was effective in inducing heat shock responses of hepatocytes. In Exp. 3, HT elevated (P < 0.01) malondialdehyde content and expressions of heat shock protein 70 (HSP70) mRNA and protein, as well as HSP90 mRNA. However, Zn supplementation increased (P < 0.05) copper zinc superoxide dismutase (CuZnSOD) activity and metallothionein mRNA expression, and effectively decreased (P < 0.05) the expressions of HSP70 mRNA and protein, as well as HSP90 mRNA. Furthermore, oZn was more effective (P < 0.05) than iZn in enhancing CuZnSOD activity of hepatocytes under HT. It was concluded that Zn (especially oZn) could alleviate heat stress of broiler hepatocytes via enhancing their antioxidant ability and attenuating heat shock responses.     

Supplementing N-carbamoylglutamate in late gestation increases newborn calf weight by enhanced placental expression of mTOR and angiogenesis factor genes in dairy cows

The objective of this study was to investigate whether supplementation with N-carbamoylglutamate (NCG) to cows during late gestation alters uteroplacental tissue nutrient transporters, calf metabolism and newborn weight. Thirty multiparous Chinese Holstein cows were used in a randomized complete block design experiment. During the last 28 d of pregnancy, cows were fed a diet without (CON) or with NCG (20 g/d per cow). The body weight of calves was weighed immediately after birth. Placentome samples were collected at parturition and used to assess mRNA expression of genes involved in transport of arginine, glucose, fatty acid and angiogenesis factors, as well as the mammalian target of rapamycin (mTOR) pathway. Blood samples of calves before colostrum consumption were also collected for the detection of plasma parameters, amino acids (AA) and metabolomics analysis. The newborn weight (P = 0.02) and plasma Arg concentration of NCG-calves was significantly higher (P = 0.05) than that of CON-calves, and the plasma concentrations of urea nitrogen tended to be lower (P = 0.10) in the NCG group. The mRNA abundance of genes involved in glucose transport (solute carrier family 2 member 3 [SLC2A3], P < 0.01), angiogenesis (nitric oxide synthase 3 [NOS3], P = 0.02), and mTOR pathway (serine/threonine-protein kinase 1 [AKT1], P = 0.10; eukaryotic translation initiation factor 4B pseudogene 1 [EIF4BP1], P = 0.08; EIF4EBP2, P = 0.04; and E74-like factor 2 [ELF2], P = 0.03) was upregulated in the placentome of NCG-supplemented cows. In addition, 17 metabolites were significantly different in the placentome of NCG-supplemented cows compared to non-supplemented cows, and these metabolites are mainly involved in arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and citrate cycle. In summary, the increased body weight of newborn calves from the NCG supplemented dairy cows may be attributed to the increased angiogenesis and uteroplacental nutrient transport and to the activated mTOR signal pathway, which may result in the increased nutrient supply to the fetus, and improved AA metabolism and urea cycle of the fetus.     

Influence of Energy and Protein Supplementation on Growth Rate,Empty Body Composition and Ruminal and Blood Metabolites of Goat Kids Fed Hay Diets

A comparative slaughter experiment was conducted with 28 Spanish kids (BW = 20.6 kg) fed a hay-based diet to determine the effects of supplemental energy and protein on growth rate, body composition, and ruminal and blood metabolites. After 84 d, kids were harvested, digesta contents removed and empty body weighed and ground. Samples were freeze-dried and analyzed for DM, CP, ether extract, ash, and gross energy. All kids had increased BW gains. However, unsupplemented kids lost empty BW and weight of all body components except ash. Kids supplemented with energy or protein increased (P<0.05) empty BW, body water, protein, fat, ash, and gross energy when compared with unsupplemented kids. The percentages of body water and ash decreased (P<0.05) when supplemental energy or protein was fed. The percentage of body protein was reduced (P<0.05) by supplemental energy and unaffected (P=0.81) by supplemental protein. Supplemental energy reduced (P<0.05) ruminal acetate and increased (P<0.05) propionate and butyrate. Supplemental protein increased (P<0.05) concentrations of ruminal ammonia N, plasma urea N, and all ruminal volatile fatty acids. Regression equations suggest that intake of 144 kcal of ME and 6.0 g of CP per kilogram of empty BW^0.75 are necessary to maintain empty BW.