共查询到20条相似文献,搜索用时 546 毫秒
1.
将猪瘟病的类型划分为标准型、非标准型和混合型三种,详细分析了每种类型的病例特征和治病机理,在此基础上从有效接种疫苗、优化饲养环境、开展针对性治疗等方面出发提出了猪瘟病的防治策略。 相似文献
2.
3.
猪瘟灭活疫苗抗体消长规律动态分析 总被引:2,自引:0,他引:2
猪瘟(CSF)是由猪瘟病毒(CSFV)引起的一种猪的急性、烈性传染病,被国际兽疫局(OIE)列为A类传染病。我国每年都投入大量的人力、物力、财力进行猪瘟疫病的研究及控制工作。但近年来猪瘟仍有发生,且有增强的趋势。至今尚无特效的治疗药物,疫苗预防仍是我国防治猪瘟的主要手段。通过对母源抗体跟踪监测和对猪瘟灭活疫苗不同免疫剂量和不同首免日龄进行比较试验,系统地反映了猪瘟母源抗体及灭活疫苗免疫后抗体的消长规律和免疫效果。为进一步掌握猪瘟灭活疫苗的免疫效力及免疫期、为该病的防治制定合理的免疫程序及为猪瘟的扑灭和净化提供科学的依据。 相似文献
4.
5.
6.
7.
《畜禽业》2021,(8)
非洲猪瘟(African swine fever, ASF)是由非洲猪瘟病毒(African swine fever virus, ASFV)引起的一种急性动物传染病,且有高度接触传染性、急性和出血性等特征,病情传播不受时间和种类的限制,其病死率高达100%。目前针对非洲猪瘟还未研究出有效的治疗方法及疫苗防控,对我国养猪业造成巨大破坏,影响经济效益。非洲猪瘟病毒是非洲猪瘟病毒科非洲猪瘟病毒属的唯一种类,是已知存在的首个虫媒病毒。非洲猪瘟病毒粒子为260~330 nm,是具有囊膜结构的二十面体双链线性DNA大分子病毒,基因组长度为170~193 kb,包含有150~167个开放阅读框,猪瘟病毒能够编码68种结构蛋白和100多种非结构蛋白。其中已知功能的病毒编码蛋白约有50个,仍有一半以上的ASFV编码蛋白功能尚未探究清楚。非洲猪瘟病毒主要寄宿于家猪和野猪体内,主要在巨噬细胞胞质中进行复制,能够调控IFN产生、炎症反应、细胞调亡、自噬及宿主蛋白合成等生物学过程,影响宿主细胞正常的生长周期及细胞分泌物的生成,进一步抑制宿主细胞体内的天然免疫应答系统。综述非洲猪瘟病毒编码蛋白种类及致病机理研究现状,为非洲猪瘟病毒结构研究及防治方法提供参考。 相似文献
8.
9.
10.
11.
12.
13.
14.
H Pittler 《Tier?rztliche Praxis》1986,14(1):55-65
Since the end of 1982 classical swine fever has again occurred in the Federal Republic of Germany. There was an increasing trend until the middle of 1984. The number of cases increased above all in the northwest of the Federal Republic, the area with a high pig density. In mid-1984 it was decided in the Federal Republic to carry out for the first time a large-scale systematic vaccination of all pigs in a certain heavily affected area--similar to what is done in the Netherlands--apart from the traditional classical measures of epizootic disease control including stamping out and quarantine; i.e. not only fattening herds (Second Regulation for the amendment of the Regulation on Classical Swine Fever of 20 June 1984--Bundesgesetzblatt I, page 766). The reasons are: in the areas with a high pig density and a high degree of infection classical measures only did no longer suffice. In addition there were trends in the EEC to bar the entire Federal Republic of Germany from exports of pigs and fresh pigmeat. Furthermore there was the danger of excluding the Federal Republic from the current EEC eradication programme, under which the Federal Republic receives considerable funds. Since it has been started to carry out systematic large-scale vaccinations of herds in infected areas in the northwest of the Federal Republic there was a clear decline in the number of new outbreaks in these areas. There are still individual cases in other regions of the Federal Republic of Germany (new outbreaks during the first half of 1984: 738 cases, the second half of 1984: 277 cases, the first half of 1985: 301 cases). Special conditions for systematic large-scale vaccination in infected areas were uniformly set up within the entire EEC. These provisions as well as other new provisions resulting from Community law were translated into national law by further amendments to the Regulation on Classical Swine Fever. Tables contain, among other things, details about pig husbandry, and the number of cases of classical swine fever in EEC member countries. 相似文献
15.
同时检测CSFV、PRRSV的二重RT-PCR方法的建立及其在临床样品检测中的初步应用 总被引:1,自引:0,他引:1
建立了1种一步法RT-PCR用于同时检测典型猪瘟病毒(CSFV)和猪繁殖呼吸道综合征病毒(PRRSV)的多重PCR诊断方法(Multiplex PCR,mPCR)。根据Genbank上公布的CSFV、PRRSV基因组全序列及部分序列,借助DNAStar和Oligo6.0基因序列和引物设计软件,设计2对特异性引物分别用于扩增CFSV、PRRSV病毒777bp和434bp的目的片断。该mPCR由RT反应和PCR反应构成。将病毒核酸连接到PMD18-T载体,提取质粒后,以10倍进行倍比稀释,取每个稀释度的病毒核酸进行mPCR反应,对CSFV、PRRSV的最低检测量分别为8.5pg、8.3×10-2pg。以PCV2、PCV1、PPV、PRV、SIV、E.coil和双蒸水为模板进行mPCR反应,扩增结果均为阴性,表明该mPCR具有较好的特异性。对临床样品的检测表明,本研究建立的mPCR诊断方法能够对CSFV和PRRSV进行快速的诊断,同时对CSFV、PRRSV在猪群中的流行病学调查也具有十分重要的意义。 相似文献
16.
2006年3月,开阳县某养猪场引进的商品猪发生一种死亡率为45%的疫病,经流行病学调查、临床症状与病理剖解观察、猪瘟荧光抗体试验和细菌分离培养与鉴定,确诊本次疫病系猪瘟与副伤寒混合感染所致。 相似文献
17.
2006年3月,开阳县某养猪场引进的商品猪发生一种死亡率为45%的疫病,经流行病学调查、临床症状与病理剖解观察、猪瘟荧光抗体试验和细菌分离培养与鉴定,确诊本次疫病系猪瘟与副伤寒混合感染所致。 相似文献
18.
Monoclonal antibodies (mab) specific for hog cholera virus (HCV), bovine viral diarrhoea virus (BVDV) or pestivirus were applied for the differential diagnosis of pestivirus infections in pigs. Field virus isolated from 8 confirmed classical swine fever outbreaks and one suspect case was propagated in PK(15) cell cultures and identified by direct immunofluorescence (IFA) and peroxidase linked antibody (PLA) assays. Peroxidase-linked HCV, BVDV and pestivirus specific mab were applied in direct PLA for differentiation. Nine isolates were classified as members of the genus pestivirus. Eight isolates showed a positive reaction with an HCV mab. One isolates reacted with BVDV specific mab only. For further characterization an indirect PLA was performed using a collection of different HCV and BVDV specific mabs. Some of the HCV isolates also showed a weak reaction with BVDV specific mab. 相似文献
19.