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采用高效液相色谱(HPLC)直接进样、梯度洗脱的分析方法,对酿酒葡萄品种赤霞珠(Vitis viniferva L.cv.Cabemet sauvignon)果实发育过程中5种主要芪化物的紫外(UV)诱导性合成进行研究.结果表明,葡萄果实在生长发育过程中,白藜芦醇(Resveratrol)和白藜芦醇的2种二聚体(ε-viniferin和δ-viniferin)呈上升趋势;经紫外诱导后,白藜芦醇、白藜芦醇糖苷(Piceid)、白藜芦醇二聚体都大量积累,白藜芦醇、白藜芦醇二聚体在葡萄花后50 d为积累的最高峰,且变化趋势基本一致,白藜芦醇糖苷的含量变化与白藜芦醇、白藜芦醇二聚体均呈现负相关.实验样品中均未检测到紫檀芪(Plerostilbene). 相似文献
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花生白藜芦醇研究进展 总被引:3,自引:0,他引:3
在花生体内,白藜芦醇合成酶是白藜芦醇生物合成途径中的关键酶之一,也是最主要的限速酶。将花生或葡萄中的白藜芦醇合成酶基因转化到其它植物中,可以有效提高植物的抗病性。本文简要介绍了白藜芦醇一般特性,对花生中白藜芦醇的分布、诱导因素作了相关介绍,并阐述了白藜芦醇在植物体内的生物合成途径及白藜芦醇合成酶基因的克隆、诱导表达及转化。 相似文献
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采用Al(NO3)3-NaNO2分光光度比色法测定菊花中总黄酮的含量,采用高效液相色谱法测定菊花中多种活性成分的含量,研究比较了雪菊与市售菊花活性成分的差异.研究表明:雪菊和市售菊花中总黄酮的含量(DW)分别为117.15~153.04 mg/g和27.05~51.82 mg/g,绿原酸、金丝桃苷、黄芪苷、槲皮素和山奈素为雪菊和市售菊花共有单体成分,而邻苯二酚、香草酸、丁香酸、芦丁仅在雪菊中检测到,白藜芦醇则仅在市售菊花中检测到.雪菊中总黄酮含量显著高于市售菊花,且酚类化合物单体组成更加丰富,含量亦相对较高,是一种药用价值更高的菊花品种,具有较高的研究和开发价值. 相似文献
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本研究选取59份花生品种,连续2年在武汉、石家庄、濮阳和周口4个地点共8个环境种植,成熟收获后检测花生籽仁白藜芦醇含量。结果表明,供试花生品种白藜芦醇含量在品种间、环境间均存在极显著差异。武汉环境下所有材料两年白藜芦醇含量均值相对较高。综合分析2年4点种植的检测结果,筛选出多个环境下白藜芦醇含量表现较高且稳定的材料2份(冀花2号和冀花13号)。结合前期对这些品种的含油量和脂肪酸检测结果的相关性分析表明,花生籽仁白藜芦醇含量与含油量和8种主要脂肪酸含量均不存在显著相关性。本结果为培育高白藜芦醇兼具高油和优良脂肪酸组成的花生品种提供了理论基础。 相似文献
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以重庆地区主栽的4个鲜食葡萄品种为试材,测定其果皮,果肉白藜芦醇含量。并用4个品种酿酒,测定其原酒的白藜芦醇含量。结果表明:4个品种白藜芦醇含量均表现为果皮>酒液>果肉。其中果皮中以蜜莉最低(19.579μg/g),与其他3个品种果皮含量差异显著;果肉中以蜜莉最高(2.093μg/g),但与其余4个品种果肉中含量差异不显著;酒液中以巨玫瑰最低(3.590μg/g),与其他酒液含量差异显著。4个品种生长、品质相关指标,以及与白藜芦醇的相关性分析表明,巨玫瑰和夏黑的品种、树龄、修剪、肥水管理等都能影响白藜芦醇的含量,同时白藜芦醇含量与葡萄其他品质也密切相关。 相似文献
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本研究利用“中花10×ICG 12625”衍生的RIL群体共140个家系及其亲本为材料,通过高效液相色谱检测各家系在2014年及2015年收获的花生种子中的白藜芦醇含量。结果表明,RIL群体白藜芦醇含量变异范围为38.27~352.08 μg/ kg。通过两年重复检测,获得稳定高白藜芦醇含量材料4份(QT0339、QT0369、QT0450和QT0454),含量为143.90~215.60 μg/ kg,在2014年环境中,这四份材料白藜芦醇含量分别超过高值亲本32.31 %、5.80 %、86.46 %和79.52 %,在2015年环境中白藜芦醇含量分别超过高值亲本106.04 %、49.78 %、7.90%和52.87%。利用前期通过该群体构建的遗传连锁图谱,应用WinQTLCart 2.5软件定位到花生种子白藜芦醇含量相关QTL13个,贡献率在2.2 %~7.4 %之间,其中qRB8.1a以及qRB8.1b为两年环境中重复检测到的QTL。本文研究结果为培育白藜芦醇含量高的花生新品种提供理论依据。 相似文献
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粮油产品真菌毒素抗体制备研究进展 总被引:2,自引:1,他引:1
本文简要综述了粮油真菌毒素抗体制备的发展情况,包括真菌毒素免疫原的制备、抗体的制备以及真菌毒素常用免疫学检测方法,同时介绍了我国真菌毒素抗体研究过程中面临的问题,并对未来真菌毒素抗体技术的发展前景进行了展望。 相似文献
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Ala’a F. Eftaiha Nidal Qinna Iyad S. Rashid Mayyas M. Al Remawi Munther R. Al Shami Tawfiq A. Arafat Adnan A. Badwan 《Marine drugs》2010,8(5):1716-1730
Metronidazole, a common antibacterial drug, was incorporated into a hydrophilic polymer matrix composed of chitosan xanthan gum mixture. Hydrogel formation of this binary chitosan-xanthan gum combination was tested for its ability to control the release of metronidazole as a drug model. This preparation (MZ-CR) was characterized by in vitro, ex vivo bioadhesion and in vivo bioavailability study. For comparison purposes a commercial extended release formulation of metronidazole (CMZ) was used as a reference. The in vitro drug-release profiles of metronidazole preparation and CMZ were similar in 0.1 M HCl and phosphate buffer pH 6.8. Moreover, metronidazole preparation and CMZ showed a similar detachment force to sheep stomach mucosa, while the bioadhesion of the metronidazole preparation was higher three times than CMZ to sheep duodenum. The results of in vivo study indicated that the absorption of metronidazole from the preparation was faster than that of CMZ. Also, MZ-CR leads to higher metronidazole Cmax and AUC relative to that of the CMZ. This increase in bioavailability might be explained by the bioadhesion of the preparation at the upper part of the small intestine that could result in an increase in the overall intestinal transit time. As a conclusion, formulating chitosan-xanthan gum mixture as a hydrophilic polymer matrix resulted in a superior pharmacokinetic parameters translated by better rate and extent of absorption of metronidazole. 相似文献
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Michalak J Gujska E Klepacka J 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):307-312
The influence that food heating has on the concentration of acrylamide in home-cooked potato dishes prepared by high-temperature,
such as pan-frying, deep-frying, roasting and microwave heating was analyzed. The experiment was performed with commercially
available deep-frozen par-fried French fries and deep-frozen par-fried potato products other than French fries (cubes, wedges,
noisettes, pancakes). Acrylamide was found in all deep-frozen par-fried French fries and other deep-frozen par-fried potato
products before domestic preparation. The mean level of acrylamide content in all frozen potato products before preparation
was found to be 322 μg/kg. Potato products were then prepared at 180 °C for 3 min and at 220 °C for 10 min. After domestic
preparation (roasting, pan-frying, deep-frying and microwave heating) it was found that the level of acrylamide in all products
increased. Acrylamide content in the analyzed samples significantly increased as the temperature and time of processing increased.
The statistical analysis showed significant differences (P < 0.05) for acrylamide content as a function of food preparation. The level of acrylamide increased with the increased heating
temperature and heating time. Additionally, the preparation method of roasting was significantly lower in acrylamide content
(P < 0.05) than the preparation method of microwaving although both preparation methods used the same conditions (time and temperature).
These results suggest that microwaving might be more favourable to the formation of acrylamide than conventional heating methods,
such as roasting. 相似文献
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综述了儿茶素酶促制备茶黄素各反应条件的研究进展,旨在为确定儿茶素酶促制备茶黄素自动化提供科学依据,并就酶促制备茶黄素在选择多酚氧化酶方面以及同工酶方面做研究展望。 相似文献
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S. Dubetz 《American Journal of Potato Research》1975,52(9):263-267
Two depths of seedbed preparation—5 and 10 in. ( 13 and 26 cm)—and two methods of fertilizer application—broadcast before plowing and banded at planting time—were compared in an irrigated experiment that was conducted for 3 years. Fertilizer, regardless of method of application, significantly increased the yield of No. 1 tubers by 27% and of total tubers by 23%. Depth of seedbed preparation had no effect on the yield or specific gravity of potatoes. On a soil that apparently does not have a compacted layer, it is not feasible to increase the yield of potatoes by deep seedbed preparation or by deep incorporation of fertilizer. 相似文献
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针对大豆田缺苗断条现象,进行调查研究分析,总结造成此现象的原因是在选种、整地、施肥、用药、播种五个关键环节存在问题:种子质量差,整地达不到标准;种肥同层施或施劣质肥;种衣剂成分、用量种类及使用方法不当;封闭除草用药不当;播种质量及时期不当等. 相似文献
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以华甜1号超甜玉米为试验材料,设置4种施肥处理,对施用硅酸盐菌剂的肥效进行了研究。结果表明:采用KCl(112.5kg/hm2) 硅酸盐菌剂(30kg/hm2)处理的玉米产量明显提高,玉米株高、叶面积、干重等生长指标均为最高值。 相似文献
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农杆菌介导的大豆子叶节遗传转化技术流程及操作要点 总被引:1,自引:1,他引:0
大豆遗传转化效率低,是大豆转基因育种亟待解决的重要问题。以大豆子叶节为外植体采用农杆菌介导法,系统地研究了大豆子叶节遗传转化各技术环节的操作要点,包括工程菌液制备、无菌苗获得及外植体制备、农杆菌的侵染及共培养、不定芽诱导、不定芽伸长、根诱导、驯化与移栽、抗性植株的获得及转基因植株的检测等,建立了一套较稳定、高效的大豆子叶节遗传转化体系。并利用该体系,将野生大豆耐盐碱相关基因A1对大豆品种绥农28、合丰50、合丰55、东农50进行遗传转化,系统地探讨了大豆基因型、影响T-DNA的转移效率的各环节、筛选剂浓度及不同基因型转化效率等几个重要因素。 相似文献