碳分子筛气调机在金冠苹果贮藏中的应用

应用碳分子筛气调机快速降O_2前附加高CO_2处理对控制金冠苹果乙烯释放水平和保持果实品质与直接用碳分子筛气调机气调同效,在生产上应用碳分子筛气调技术没有必要附加高CO_2处理,气调帐(库)内也不必加消石灰来吸收CO_2。     

苹果双变气调贮藏理论与技术初探

本研究对苹果的气调贮藏提出了一种可以节省设施投资,能够结合利用自然低温,降低贮藏技术成本的双相变动气调贮藏理论与技术途径。试验结果表明:新红星苹果采用双相气调(10～0℃,CO_2 12～6％,O_2 3％)、青香蕉苹果采用高温(10℃)高CO_2(6或12％)气调贮藏5～6个月后的保鲜效果和营养成分均优于O℃冷藏,接近标准气调贮藏(0℃,CO_23％,O_2 3％)。试验苹果在高温高CO_2或双变气调条件下,呼吸显著减弱,乙烯发生量显著减少,果皮中的共轭三烯含量显著降低,无虎皮病发生。     

肥城桃的气调贮藏研究初报

肥城桃的贮藏保鲜以碳分子筛气调贮藏效果最好。在气调贮藏条什下,O_2浓度一定时,CO_2浓度与果肉褐变呈正相关;相同CO_2浓度下,O_2浓度增加则加剧桃果实衰老。以低氧低二氧化碳(O_23～4％、CO_22～3％)最适宜于肥城挑的气调贮藏。     

苹果双变气调生理基础的探讨

双变气调以低O_2高CO_2处理克服或抑制贮藏初期较高温度的不利影响.试验结果表明,在10—15℃高温度下低O_2高CO_2处理果实的呼吸强度和果内乙烯浓度较低;在温度和CO_2浓度协同降低后,处理果实的呼吸强度和果内乙烯浓度持续较低,其果皮α-法尼烯和共轭三烯含量明显低于冷藏对照.     

延迟和缩短苹果气调处理时间对贮藏效果的影响

 以新红星苹果为试材探讨延迟和缩短低氧气调新组合(10 ℃, 2 %O₂ + 7. 5 %CO₂) 处理时间对其贮藏效果的影响。结果表明, 贮藏123 d 时, 冷藏对照(0 ℃, 空气) 和常规气调处理(0 ℃, 3 %O₂ + 3 %CO₂) 果实的虎皮病果率分别为25. 6 %和1. 02 % , 果肉硬度分别为5. 79 和6. 52 kg/ cm², 而低氧新组合处理无虎皮病, 果肉硬度(6. 63 kg/ cm²) 显著高于冷藏对照, 而与常规气调处理无显著差异。10 ℃空气中放15 d 后再用低氧新组合处理108 d 的果实虎皮病果率6. 67 % , 果肉硬度(4. 8 kg/ cm²) 显著低于冷藏对照和常规气调处理。这些结果说明果实采后及时进行低氧气调处理是必要的。冷藏对照、常规气调处理和在10 ℃下低氧新组合处理123 d 的果实再于0 ℃空气中放58 d , 其虎皮病果率分别为46. 9 %、36. 4 %和0 , 果肉硬度分别为5. 28 、5. 21 和4. 5 kg/ cm²。在10 ℃下低氧新组合处理96 d 再冷藏的苹果, 在贮后50 d 时无虎皮病果, 果肉硬度为4. 67 kg/ cm² 。综合来看, 计划贮期约5 个月时, 10 ℃下低氧新组合处理的时间可由4 个月缩短为3. 3 个月。低氧气调新组合处理的苹果在气调期间α2法尼烯和共轭三烯的形成受到显著抑制。     

新红星苹果双相变动气调贮藏研究

以人工气调配气系统为半定量式气调手段,研究新红星苹果在变动温度与变动 CO_2浓度的双相变气调条件下的果实品质和生理生化变化.结果表明,双变气调的最佳指标是:温度从10℃渐降至0℃,CO_2从12%—15%渐降至3%,O_2为3%.在此条件下,新红星苹果贮藏185d,果肉硬度高达7.2kg·cm~(-2).能明显地抑制呼吸、原果胶水解、乙烯合成和α—法尼烯生成.     

鸭梨果实气调贮藏的研究

鸭梨果实采后在20±1℃常温下具有典型的呼吸跃变过程,跃变峰过后,果实硬度有所下降,可溶性固形物及可滴定酸含量在常温下23天无显著变化。 采用缓慢降温(12→0℃)结合 7-10％O_2 0％CO_2进行气调贮藏,可以显著减少鸭梨果实晚期黑心病的发生率,经213天贮藏后,7％O_2 0％CO_2,10％O_2 0％CO_2和空气对照果的果心褐变指数分别为0.075,0.075和0.192;与对照相比,气调处理可以维持较高的果肉硬度和可滴定酸含量,减少失重率,延长货架寿命,对可溶性固形物无显著影响。7％O_2 0％CO_2处理优于10％O_2 0％CO_2处理。     

气调贮藏中气体分离器技术的开发应用(连载之二)

出库时和在20℃下置放7天后对虎皮病和其它生理失调症进行了评估。可以明显地看出,第一期采取的果实其虎皮病发生率高,采用活性炭洗涤器气调贮藏的果实贮藏期间约55％的发生虎皮病。但在Clarksville园艺试验     

气调贮藏控制虎皮病的方法

气调贮藏是控制虎皮病发生的有效非化学防治方法之一[1].尽管二苯胺和乙氧基喹是商业上应用的、有效的虎皮病抑制剂,但它们的前景不容乐观[2].近年来消费者越来越不接受化学药剂的使用,二苯胺在某些欧洲国家已被禁用,在美国也是前途未卜[3].     

新红星苹果高CO_2处理的最佳指标研究

试验以定量式气调手段,研究新红星苹果高 CO_2处理的直接效应和残留效应。两年的研究结果表明:新红星苹果通风库 MA 贮藏的高 CO_2处理的最佳指标是25%-30%CO_2处理46天。处理后的果实于通风库内 MA(自发气调)贮藏5个多月(采后194天)。果实无CO_2伤害,品质好,果肉硬度高达6.2kg/cm~,虎皮病指数为零,贮效极显著地高于对照。     

Ergebnisse der Leistungsprüfungen der Süßkirschensorte ‚Stella‘ auf Gisela- und Weiroot-Unterlagen in Bulgarien

Zusammenfassung Die Leistungsprüfungen wurden im Zeitraum 1997 bis 2003 mit den Unterlagen Gisela 4 und 5, den Klonnummern 195/20 und 497/8 aus der Gisela-Serie sowie Weiroot 10, 13, 53, 72 und 158 durchgeführt. Dabei dienten Sämlinge von P1 (bulgarische Selektion aus Prunus mahaleb) als Kontrolle. Alle Unterlagen waren mit der Sorte Stella veredelt und im Dezember 1996 in der Versuchsanlage der Agraruniversität in Plovdiv, Bulgarien, im Abstand von 6 m×4,5 m gepflanzt worden. Dabei erfolgte ein Pflanzschnitt. Nach Abschluss der natürlichen Kronenentwicklung wurde jedes Jahr ein Winterschnitt vorgenommen. Der Boden wurde durch mechanische Bearbeitung offen gehalten und nach dem 4. Standjahr wurden die Baumstreifen mit Herbiziden behandelt. Die Wasserversorgung erfolgte durch eine dem natürlichen Gefälle folgende Überflutung, allerdings nicht immer zum optimalen Zeitpunkt, da keine eigene Wasserquelle zur Verfügung stand.Basierend auf den Ergebnissen bis zum Anfang des 7. Standjahres können die untersuchten Unterlagen in zwei Gruppen differenziert werden: starkwüchsig—Weiroot 10, P1 und Weiroot 13; mittelstarkwachsend bis schwachwüchsig—Gi 497/8, Gisela 4, Weiroot 53, Weiroot 158, Gi 195/20, Weiroot 72 und Gisela 5. Letztere zeichnete sich durch besondere Schwachwüchsigkeit aus. Die meisten Wurzelschosser bildeten Gisela 4, Weiroot 10 und Weiroot 13. Weiroot 53, Weiroot 72 und Weiroot 158 entwickelten deutlich weniger und P1, Gisela 5, Gi 195/20 sowie Gi 497/8 keine Wurzelschosser. Den frühesten Blühbeginn induzierte Gisela 4. Die anderen Unterlagen führten, in Abhängigkeit von den Temperaturbedingungen des jeweiligen Jahres, zu einer Verspätung der Blüte: P1 und Weiroot 10 um 1–2 Tage; Gi 497/8, Weiroot 13 und Weiroot 158 um 2–4 Tage; Weiroot 72 um 2–7 Tage; Gi 195/20 um 3–6 Tage; Weiroot 53 um 3–8 Tage und Gisela 5 um 3–10 Tage. Die Reifezeit der Früchte war bei den Bäumen auf Gisela 5 im Vergleich zu den anderen Varianten um 2–3 Tage verspätet. Gisela 5, Weiroot 72 und Gisela 4 induzierten bei der aufveredelten Sorte die höchsten Ertragsleistungen, P1 die geringsten. Bei den Bäumen auf Gisela 5 war die Fruchtgröße geringer als bei den anderen Unterlagen. Bäume auf Gisela 5 brauchen intensive Pflege. Nur wenn alle Produktionsfaktoren und kulturtechnischen Maßnahmen optimiert werden, kann das hohe Ertragspotenzial dieser Unterlage ausgeschöpft werden.     

Relationship between renal hypertension and cardiac α1-adrenoceptor autoantibodies in rats

AIM: To examine the autoantibody against α₁-adrenoceptor and its biologic activities during the development of renal hypertension. METHODS: Renal hypertension of rat was achieved by clipped renal artery, the titre of autoantibody to α₁-adrenoceptor was detected using ELISA immunoassay. Furthermore, the biological offects of these autoantibodies on cultured cardiomyocytes were also examined. RESULTS: After two weeks of clipping renal arteries, both the frequency of occurrence and the titre of autoantibodies to cardiac α₁-adrenergic receptor were significantly increased as compared with the control of pre-treatment. The increased autoantibodies lasted for several weeks and then automatically decreased gradually to the pre-clipping level at 12 weeks. The biological effects of these autoantibodies displayed an "agonistic-like" activities on the beating frequency of cultured neonatal cardiomyocytes. CONCLUSION: Autoantibodies against α₁-adrenoceptor may play a role in the elevation of peripheral vascular resistance and in the development of cardiac hypertrophy in rats with renal hypertension.     

Effects of radiation from 188Re on smooth muscle cell proliferation

AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β^-particles emission from ¹⁸⁸Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by ¹⁸⁸Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [³H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of ¹⁸⁸Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from ¹⁸⁸ Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G₀/G₁ arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation.     

Influence of Mineral Nutrients on Strawberry Fruit Quality and Their Accumulation in Plant Organs

Abstract

This review is based partly on complete articles and partly on abstracts. Three of the 60 articles deal with the total uptake of elements in strawberry plant organs in two different strawberry production systems, both considered as optimal concerning amount and balance of elements. The effect on fruit quality may be dramatic if the level of a particular element is outside this range, but there may also be effects initiated by differences within the optimal range of elements. Most articles refer to product oriented quality, but some focus on consumer oriented quality, as discussed by Shewfelt (1999). The discussion here is on a general basis, so one should keep in mind that there are cultivar differences and that specification of nutrition ideally should mirror the needs of a single cultivar, or a group of cultivars with similar requirements. Also, to get a complete understanding of the subject future reviews should embrace a broader access of information including the effect on plant development of individual elements, such as the role of calcium in fruit firmness and its importance in cell wall structure. However, the intention here is to narrow the information to results that suggest a direct connection between nutrient uptake and fruit quality.     

Effect of L-Arg on plasma content of endothelin and expression of c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy

AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration.     

Effect of puerarin on pulmonary arterioles wall collagen metabolism of chronic hypoxia hypercapnia rats

AIM:To investigate the effect of puerarin on pulmonary vessel collagen metabolism in pulmonary hypertension rats induced by chronic hypoxia and hypercapnia.METHODS:Collagen Ⅰ, Ⅲ and their mRNA were observed in pulmonary arterioles by the technique of immunohistochemistry and in situ hybridization.RESULTS:① Light microscopy showed media thickness of pulmonary arterioles was much higher in HH(hypoxic-hypercapnia) group than that of NC(normal control) group, and, vessel cavity turned more straiter in HH group than that of NC group.However, the damage of pulmonary arterioles in HP(hypoxic-pueratin) group was much slighter than that of HH group. ② The levels of plasma ET-1 and lung homogenates Hyr were much higher in HH group than those of NC group(P＜0.01), and lower in HP group than HH groups(P＜0.01).Plasma NO content in group HH was lower than that of group NC(P＜0.01), it was higher in group HP than that of group HH(P＜0.01).③Expression of collagen Ⅰ and collagen Ⅰ mRNA in pulmonary arterioles were significantly higher in HH groups than those of NC group (P＜0.01), and they were lower in HP group than those of HH group (P＜0.01).Expression of collagen Ⅲ and collagen Ⅲ mRNA showed no difference among three groups(P＞0.05).CONCLUSION:Puerarin inhibited the deposition of collagen and improved pulmonary vessel remodeling.     

Mixed-severity fire regime in a high-elevation forest of Grand Canyon,Arizona, USA

Fire regime characteristics of high-elevation forests on the North Rim of the Grand Canyon, Arizona, were reconstructed from fire scar analysis, remote sensing, tree age, and forest structure measurements, a first attempt at detailed reconstruction of the transition from surface to stand-replacing fire patterns in the Southwest. Tree densities and fire-/non-fire-initiated groups were highly mixed over the landscape, so distinct fire-created stands could not be delineated from satellite imagery or the oldest available aerial photos. Surface fires were common from 1700 to 1879 in the 4,400 ha site, especially on S and W aspects. Fire dates frequently coincided with fire dates measured at study sites at lower elevation, suggesting that pre-1880 fire sizes may have been very large. Large fires, those scarring 25% or more of the sample trees, were relatively infrequent, averaging 31 years between burns. Four of the five major regional fire years occurred in the 1700s, followed by a 94-year gap until 1879. Fires typically occurred in significantly dry years (Palmer Drought Stress Index), with severe drought in major regional fire years. Currently the forest is predominantly spruce-fir, mixed conifer, and aspen. In contrast, dendroecological reconstruction of past forest structure showed that the forest in 1880 was very open, corresponding closely with historical (1910) accounts of severe fires leaving partially denuded landscapes. Age structure and species composition were used to classify sampling points into fire-initiated and non-fire-initiated groups. Tree groups on nearly 60% of the plots were fire-initiated; the oldest such groups appeared to have originated after severe fires in 1782 or 1785. In 1880, all fire-initiated groups were less than 100 years old and nearly 25% of the groups were less than 20 years old. Non-fire-initiated groups were significantly older (oldest 262 years in 1880), dominated by ponderosa pine, Douglas-fir, or white fir, and occurred preferentially on S and W slopes. The mixed-severity fire regime, transitioning from lower-elevation surface fires to mixed surface and stand-replacing fire at higher elevations, appeared not to have been stable over the temporal and spatial scales of this study. Information about historical fire regime and forest structure is valuable for managers but the information is probably less specific and stable for high-elevation forests than for low-elevation ponderosa pine forests.This revised version was published online in May 2005 with corrections to the Cover Date.     

Compositional and Functional Properties of Saskatoon Berry and Blueberry

Abstract

Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared.     

Cryopreservation of shoot apices of hawthorn in vitro cultures originating from East Asia

The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species.     

瓜叶绿素降解关键酶基因PPH和PAO cDNA片段的克隆与表达初步分析

 以西双版纳黄瓜和‘华黄5号’黄瓜叶片为试验材料,研究了衰老过程中两种黄瓜叶片叶绿素含量变化及脱镁叶绿素脱镁叶绿酸水解酶基因PPH和脱镁叶绿酸a加氧酶基因PAO的表达。结果表明：随着叶片衰老,叶绿素a和叶绿素b含量显著降低,叶绿素a与叶绿素b的比值在0.5左右,推测叶绿素b的含量可能对黄瓜叶片保绿起到重要作用。荧光定量RT-PCR分析结果显示：PPH和PAO在西双版纳黄瓜叶片生长35 d时表达量最高,分别为5.50和1.86;PPH在‘华黄5号’叶片35 d时表达量最高,为7.14,而PAO在35和45 d表达量都很高,分别为3.90和3.91;PPH和PAO在西双版纳黄瓜叶片中的表达量低于在‘华黄5号’中。