Effects of the Long-Term Application of Anaerobically-Digested Cattle Manure on Growth,Yield and Nitrogen Uptake of Paddy Rice (Oryza sativa L.), and Soil Fertility in Warmer Region of Japan

Abstract

The suitability of anaerobically-digested manure (ADM) from a biogas plant, as an alternative to chemical fertilizers for rice cultivation was evaluated by a long-term study. At the standard nitrogen (N) application rate (10 g m^?2), the aboveground biomass, N uptake, and grain yield in rice plots with ADM application (MF) were not significantly different from those in the plots treated with chemical fertilizer (CF). Split application of ADM improved the apparent N efficiency. The N application rate corresponding to maximum grain yield was approximately 15 g m^?2 by a split application, and more N application by using ADM saturated grain yield due to decrease in ripened grain ratio and individual grain weight. On the whole, the soil total-C, total-N and available N in the MF plot were not significantly different from those in the CF plot. The available phosphate (P) levels was lower in the MF plot than in either the CF plot or plot without N application (NF), mainly due to lower content of P in ADM. However, the P level remained much higher than the fatal threshold level for the growth of paddy rice. These findings suggest that under appropriate fertilization conditions, ADM is a valuable organic resource, and can be used continuously as an alternative to chemical fertilization for rice cultivation, without substantial changes in soil C and N fertility.     

Influence of Oil Palm Empty Fruit Bunch Biochar on Floodwater pH and Yield Components of Rice Cultivated on Acid Sulphate Soil under Rice Intensification Practices

Abstract

Rice has a vital role in food security but the production is limited in infertile and degraded soils. Rice is cultivated on acid sulphate soil in the coastal area of Peninsular Malaysia. Soil amendment using biological charcoal (biochar) increases the soil fertility. Thus, empty fruit bunch biochar (EFBB) was applied in a pot experiment under a controlled environment using an organic system of rice intensification (SRI) practice and its effects on the floodwater pH, acid sulphate soil properties and growth performance of rice and yield of rice MR219 were preliminarily investigated. EFBB increased grain yield by 141 to 472%. Plant growth and yield parameters in EFBB amended soils were significantly higher than in soil without biochar. The number of tillers increased significantly with the increase in biochar applied; 28 tillers were produced in the control, while up to 80 tillers were produced in the plots applied 40 t ha^–1 EFBB. Moreover, the decline of Al³⁺ in flood water indicated that EFBB mitigated Al³⁺ toxicity. Soil water pH increased from 3.5 to 6 with increasing EFBB application rates. The grain yield was linearly correlated to the application rate of EFBB. This pot study demonstrates that the application of EFBB combined with organic fertilization and intermittent irrigation has the potential to improve rice yield on acid sulphate soil. Further study in the field is warranted to determine the effect of EFBB on large scale rice production.     

有机无机肥配施对冬水田水稻产量和耕层土壤性质的影响

在农业生产要求全面减施化肥农药的大背景下,综合评价四川冬水稻田区有机无机配施对水稻产量和土壤肥力的效应具有一定意义。本试验在冬水稻田区,比较了在等氮量替代条件下,无N(CK)、单施化肥N、P、K(T1)、30%有机肥N+70%化肥N(T2)、50%有机肥N+50%化肥N(T3)、100%有机肥N(T4)等处理的水稻产量、氮肥利用和土壤肥力变化情况。结果表明,有机无机肥配施处理的水稻产量较CK增加17.29%~31.43%,T2处理最高,且明显提高了氮肥利用率、氮肥农学效率和氮肥偏生产力;有机无机肥配施具有提升土壤有机质、培肥地力的作用。可见,冬水稻田区适宜的有机肥替代方案为30%有机N+70%化肥N,既可稳定水稻产量、降低成本,又可提高氮素利用率和降低对农田环境的不良影响。     

Higher yields of hybrid rice do not depend on nitrogen fertilization under moderate to high soil fertility conditions

Background

Increasing rice yield with fewer external inputs is critical to ensuring food security, reducing environmental costs, and improving returns. Use of hybrid rice has expanded greatly in China due to its higher yield potential. Meanwhile, large and increasing amounts of nitrogen (N) fertilizers have been used for expanding rice production in China. It is not clear to what extent the success of hybrid rice in China is associated with N fertilizer inputs.

Findings

We observed that the higher grain yield with N fertilizer in hybrid rice was driven more by a higher yield without N fertilizer than by increases in grain yield with N fertilizer under moderate to high soil fertility conditions.

Conclusions

Our results suggest that greater application of N fertilizers is not needed to benefit from hybrid rice production under moderate to high soil fertility conditions, and that improving and maintaining soil fertility should be a focus for sustaining hybrid rice production. Moreover, our study also indicates that zero-N testing may be a potentially useful tool to develop hybrid rice with high yield and without requirement of greater external N inputs under moderate to high soil fertility conditions.

     

Nitrogen Uptake by the Rice Plant and Changes in the Soil Chemical Properties in the Paddy Rice Field during Yearly Application of Anaerobically-Digested Manure for Seven Years

Abstract

This study investigated the changes in the chemical properties of the plow soil during a 7-year period with yearly application of anaerobically-digested manure (ADM), and the effect of the different ADM application methods on nitrogen (N) uptake by rice plants and the apparent N balance (input N minus output N). Among the treatments significant differences were observed in the pH, total N, and available N in the plow soil. The cumulative N uptake by rice plants in the ADM split application plots was significantly higher than that in single application plots. Consequently, the apparent N balance (input N minus output N) in the single ADM application plots was more positive. This result suggests that split application of ADM is more favorable for N uptake by rice plants. Although the apparent N balance was negative in the plots without N fertilizer, with chemical N and with low ADM, there was no observable decline in the total N and available N in the plow soil. This result implies that input and output of N in these plots were well balanced by additional N supply other than fertilization. On the other hand, the apparent N balance during the 7-year study under standard and heavy application of ADM was positive, indicating the preservation of soil fertility.     

长期有机肥无机肥配施对水稻氮素吸收、转运及产量的影响

基于25年定位试验,设置6个大田试验处理（CK,不施肥;NPK,常规施用化肥;1/4M+3/4NPK,25%有机肥替代化肥;1/2M+1/2NPK, 50%有机肥替代化肥;M,100%有机肥替代化肥;MNPK, 100%化肥+100%有机肥）,研究了长期有机肥无机肥配施对水稻氮素吸收和利用的影响。结果表明,水稻地上部N素总积累量与产量大小均表现为MNPK>1/4M+3/4NPK> NPK>1/2M+1/2NPK>M>CK,与NPK处理相比,MNPK和1/4M+3/4NPK处理的地上部氮素总积累量增加20.11%~27.86%、产量增加2.04%~12.42%。各处理花前N素转运量对籽粒贡献率（69.54%~84.60%）显著高于花后积累量对籽粒贡献率（15.40%~30.46%）,花前N素积累是水稻植株N素的主要来源。1/4M+3/4NPK处理的氮肥偏生产力及氮收获指数均高于NPK处理,分别提高16.42%、10.29%。有机无机肥配施比单施化肥处理显著改善了稻米品质,提高了籽粒氨基酸含量,其中,非必需氨基酸含量是必需氨基酸含量的2倍左右。长期施肥条件下,25%有机肥替代化肥处理在化肥减施基础上,可以提高肥料利用率、促进水稻养分吸收,是黄壤性水稻土地区实现水稻增产、品质提升的最佳施肥措施。     

Wheat grain yield,phosphorus uptake and soil phosphorus fraction after 23 years of annual fertilizer application to an Andosol

A field experiment was conducted on an Andosol to evaluate wheat (Triticum aestivum L.) yield, P and N uptake and soil P fraction after long-term fertilization (no fertilizer, NPK, NP, NK and PK treatments). Application rates of N, P and K fertilizers were 100, 65 and 83 kg ha⁻¹ year⁻¹ by ammonium sulfate, superphosphate, and potassium chloride, respectively. Phosphorus fertilization was critical for grain yield since the NK treatment did not increase yield compared with no fertilizer treatment. Agronomic efficiency of P was greater than agronomic efficiency of N, although apparent recovery of P and N were 17 and 53%, respectively. Combination application of fertilizer P and N resulted in the greatest grain yield over 23-year cultivation. Interaction impact on grain yield between P and N ranged from 71 to 109%, and was greater than the values for cereals in the earlier works. The N/P ratios of wheat decreased by P application and increased by N application. The N/P ratios in NPK and NP treatments were higher than the values attaining maximum yield for cereal crops reported by other works.     

Bahiagrass response and N loss from selected N fertilizer sources

The increasing cost of commercial fertilizers and environmental problems associated with improper fertilization management have prompted the need to re‐examine commercial N sources that can effectively supply N to pastures while minimizing N losses. This 3‐year study evaluated the effects of selected N sources on bahiagrass (Paspalum notatum Flügge) responses, soil properties and N losses. Treatments consisted of a factorial combination of 6 N sources [(i) ammonium nitrate (AN), (ii) ammonium sulphate (AS), (iii) urea (U), (iv) urea treated with Agrotain (U + Agrotain), (v) SuperU and (vi) ammonium sulphate nitrate] and 3 N levels (0, 60 or 120 kg ha^?1 year^?1), replicated three times. Bahiagrass dry‐matter yield (DMY), crude protein (CP) concentration, N uptake and recovery were not affected by N source, with the exception of AN that resulted in reduced DMY in 2010 compared with the other sources. Bahiagrass DMY, CP concentration, N uptake and recovery increased linearly as N levels increased. Nitrogen fertilization showed no effect on soil pH or soil N accumulation. Soil pore‐water N concentrations from treatments fertilized with N were similar to the control plots indicating no threat to the environment. At the N levels evaluated in this study, selection of N source should be based on the fertilizer cost.     

Fate of 15N-labeled Inorganic Fertilizer in an Upland Soil Applied with Sweet Sorghum Bagasse and N Uptake Efficiency by Komatsuna Plants

Abstract

Sweet sorghum bagasse (SSB) is a soil amendment with potential for biofuel production. This study was conducted to determine the appropriate techniques for application of SSB and the effect of incorporation of inorganic fertilizer (IF) on the production of komatuna (Brassica rapa) plants. SSB was applied to the surface of the plant or incorporated into soil. The N fate of IF was evaluated by using ¹⁵N-labeled IF. The combination of surface application of SSB and incorporation of IF to soil decreased the N uptake by komatsuna plants but increased dry weight, whereas the incorporation of IF and SSB gave lower komatsuna dry weight than IF treatment alone. Moreover, the application of SSB tended to increase the N distribution from IF to komatsuna with decreased N loss from the plant-soil system. These results showed that surface application of SSB is effective for increasing crop production due to reduction of N loss and improved N use efficiency.     

Effect of a Bio-decomposer on Utilization by Rice (Oryza sativa L.) of 15N Derived from Rice Chaff or Straw

Abstract

The ¹⁵N - labeled technique was used to study the stock, transformation, fate and utilization efficiency of N in the farming–pig husbandry–biogas ecosystem in rice areas. It was shown that the crude protein digestibility of the ensilaged milk vetch by pig was 53.76%, the recovery rates of ensilaged milk vetch N from the pig feces and urine were 39.36% and 24.71%, respectively. The recovery rate of biogas fermentation N of pig feces and urine was 97.9%. The quantity of alkali-hydrolysable N was 2.6 times as great as before the fermentation. The mixed application of milk vetch, biogas-tank sludge and chemical fertilizer could promote N partitioning to rice grain, therefore it was advantageous to yield improvement. The residual quantity in soil of the fertilizer N was equivalent to 2.0 - 2.5 times that of only chemical fertilizer and yet gaseous loss N of the latter was 2.6-8.2 times that of the former. The comprehensive economic effect of the whole ecosystem increased by times, compared with the only milk vetch-early rice-late rice pattern, and ecological effect and social effect were also very prominent.     

测土配方施肥对水稻生长发育及产量形成的影响

以当地优质稻南粳5718和糯稻99-25为供试材料,根据试验地土壤养分状况,采取减氮增磷增钾的措施,即在基肥中减少氮肥,增加磷钾肥,并在追肥中降低氮肥投入,来研究测土配方施肥技术对当地水稻增产的途径和方法。结果表明,测土配方施肥技术能够通过促进水稻分蘖,提高成穗数,其中南粳5718高峰苗较常规施肥处理增加8.02%,有效茎蘖数增加5.26%,穗数增加4.89%;糯稻99-25高峰苗较常规施肥处理增加10.12%,有效茎蘖数增加4.32%,穗数增加2.34%。测土配方施肥可以提高抽穗期的叶面积指数(增幅19.47%),强化水稻的光合能力。在不影响穗粒数和结实率的情况下,测土配方施肥可以增加水稻千粒质量,从而最终提高产量。     

基于鄂北区域的两种水稻缓控释肥用量研究

采用田间试验,研究了两种缓控释肥料施用对鄂北水稻产量和产量构成因子、养分吸收及肥料利用率的影响,比较了两种缓控释肥料不同用量施用效果的差异,以期为鄂北水稻生产中缓控释肥的合理施用提供理论依据.结果表明,施用缓控释肥料可显著提高水稻产量.与不施肥处理相比,施用掺混包膜型控释复合肥料处理水稻增产6.6％～17.3％,施用腐...     

Integrated Nitrogen Fertilization for Intensive and Sustainable Agriculture

Summary

Increased efficiency of land use and resource-use are critical for agriculture to feed the ever increasing population and yet remain sustainable for the future generations. Mineral fertilizers, particularly, nitrogen (N) fertilizer has played a very important role in meeting the increased food demand and in saving millions of hectares of marginal and wildlife reserves from coming under cultivation. Apart from higher yields per unit land, N application also results in higher protein content. Improper use of N fertilizers due to high application rates, incorrect source and method of application, and poor timing of application have led to air and water pollution and economic losses. Integrated and balanced N fertilization results in a win-win situation with intensive and sustainable agriculture that feed the world without harming the environment. Since multitude of management, soil, crop, and weather-related factors control soil and plant N dynamics, N management recommendations based on field trials alone are too costly and time-consuming. Dynamic N management recommendations that are both site— and season-specific combine field trials with decision support systems (DSS) that simulate crop growth and N transformations as a function of above-mentioned factors. Innovative N fertilizer products and N efficient plant types will further improve N use efficiency.     

A Knowledge-Based Model for Nitrogen Management in Rice and Wheat

Abstract

Excessive nitrogen fertilization results in low nitrogen-use efficiency. To improve nitrogen management for high yield and high nitrogen efficiency in rice and wheat, we developed a knowledge-based nitrogen fertilization model by integrating the quantitative relationship between N fertilization and yield target with respect to N supply and demand balance. The total amount of nitrogen and ratio of basal to top dressing nitrogen could be determined by this nitrogen fertilization model, and the desirable nitrogen fertilizer strategies could be made under the conditions of different climates, soil types and managements. Furthermore, the function of dynamic regulation of pre-designed N dressing rate could be determined by using the nitrogen fertilization model on the basis of actual growth status under a specific production system. The nitrogen fertilization model is evaluated using the data from field experiments of rice and wheat at Nanjing, and the results on crop growth pattern and N use showed that the grain yield and N recovery were markedly improved by the N fertilization plan given by the model. The nitrogen fertilization model can be used as guidance for quantifying N fertilization strategy in cereal crop production.     

Nitrogen Uptake by Faba Bean from 15N-Labelled Oilseed-Rape Residue and Chicken Manure with Ryegrass as a Reference Crop

Abstract

The effects of soil amendment with oilseed-rape residue (OSRR) and chicken manure (CM) on the growth and nitrogen (N) uptake of faba bean (Vicia faba L.) were assessed in a pot experiments with Italian ryegrass (Lolium multiflorum Lam.) as a reference crop. A ¹⁵N isotope dilution method was used to estimate the amount of N derived from the residue (OSRR and CM) and from atmosphere through N₂ fixation in the plants. Dry weights (DW) of shoots and whole plants were heaviest in the plants grown on the soil amended with CM (CM plants) followed by the plants grown on the soil amended with OSRR (OSRR plants) and control plants in this order. There were significant differences (p<0.05) in dry weight between CM, OSRR and control plants. DW of roots was also increased by amendment with either CM or OSRR in faba bean, but it was decreased in ryegrass. The amount of total N in both roots and shoots were increased by application of either CM or OSRR in both faba bean and ryegrass. The amount of N₂ fixed by faba bean cultured on 1.2 kg soil amended with 10g residue (CM or OSRR) was 85.9 mg pot-¹ but total N in faba bean derived from OSRR and CM was 192 and 374 mg pot-¹, respectively. The percentage of N derived from atmosphere to total N in faba bean plants ranged from 15.9 to 26.5%. The amount of N taken up by faba bean and ryegrass plants from CM were larger than those from OSRR by 81.0 and 54.3%, respectively. Soil N balance was calculated as the difference between the amount of N applied (including fixed) and taken up by the plants. The N balance of soil amended with OSRR after cultivation of faba bean was 72.2% higher than that of the soil amended with CM, and that after cultivation of ryegrass was 89.9% higher.     

Characterizing N uptake and use efficiency in rice as influenced by environments

To compare N uptake and use efficiency of rice among different environments and quantify the contributions of indigenous soil and applied N to N uptake and use efficiency, field experiments were conducted in five sites in five provinces of China in 2012 and 2013. Four cultivars were grown under three N treatments in each site. Average total N uptake was 10–12 g m^?2 in Huaiji, Binyang, and Haikou, 20 g m^?2 in Changsha, and 23 g m^?2 in Xingyi. Rice crops took up 54.6–61.7% of total plant N from soil in Huaiji, Binyang, and Haikou, 64.3% in Changsha, and 63.5% in Xingyi. Partial factor productivity of applied N and recovery efficiency of applied N in Changsha were higher than in Huaiji, Binyang, and Haikou, but were lower than in Xingyi. Physiological efficiency of soil N and fertilizer N were lower in Changsha than in Huaiji, Binyang, and Haikou, while the difference in them between Changsha and Xingyi were small or inconsistent. Average grain yields were 6.5–7.5 t ha^?1 (medium yield) in Huaiji, Binyang, and Haikou, 9.0 t ha^?1 (high yield) in Changsha, and 12.0 t ha^?1 (super high yield) in Xingyi. Our results suggest that both indigenous soil and applied N were key factors for improving rice yield from medium to high level, while a further improvement to super high yield indigenous soil N was more important than fertilizer N, and a simultaneous increasing grain yield and N use efficiency can be achieved using SPAD-based practice in rice production.     

优化施肥效应模型对佛手产量和品质的影响

本研究以适宜在万州种植的佛手为研究对象，采用“3414”不完全区组正交设计的N、P、K三因子施肥试验，选取最优施肥效应模型进行验证，并以佛手的单果干重和橙皮苷含量为指标进行统计分析。结果表明：N、P、K肥对佛手单果干重和佛手中橙皮苷含量有影响，在不同施肥处理中N2P2K2(N为40 g/株、P为40 g/株、K为45 g/株)的处理效果最佳，不施或者少施肥均不同程度导致万州佛手的品质下降；单因子效应分析表明，随着氮、磷和钾施肥量的提高，佛手单果干重和橙皮苷含量呈先升后降的趋势；双因素交互效应分析表明，当N、P、K肥任一因素处于2水平时，随着另外2个因素施肥量的增加，佛手单果干重和橙皮苷含量均表现为先增加后减少的趋势，说明任意2个因素在一定范围内存在着相互作用，N、P、K三因子中两因子存在着交互效应，对万州种植佛手产量和橙皮苷的含量有一定影响；施肥模型优化结果表明，万州佛手栽培N、P、K肥的最佳施用量分别为38.78～51.25、35.67～42.35、48.17～60.37 g/株。在最优施肥效应模型中间值条件下验证实验，结果显示佛手单果干重产量较“3414”组的最高组高出8.14%，...     

氮磷钾肥对红壤区水稻增产效应的影响

通过红壤区水稻试验,分析了长期使用氮磷钾肥条件下双季水稻的生物量（包括稻谷产量和秸秆量）、养分增产效应和土壤肥力的变化。氮磷钾肥的配合施用对双季水稻生物量的影响顺序为NPK>NP>PK>NK>CK。N、P、K对水稻的增产效应系数分别为41.2%~54.8%、66.3%~88.2%和10.3%~10.9%。N、P和K增产效应的变异系数规律与其增产效应相反,说明养分对双季水稻增产效应越高,其稳定性越好。双季水稻的增产效应及其稳定性均表现为早稻优于晚稻。氮磷钾肥的配施可显著提高土壤有机质和全氮含量。磷肥的使用明显增加了土壤全磷和有效磷含量。钾肥使用提高了土壤全钾和交换性钾含量,然而水稻生物量的输出却与之相反,两者的平衡决定着土壤全钾和交换性钾含量的变化。氮磷钾肥对土壤pH值影响则不一致,氮肥（尿素）的长期使用降低土壤pH值,而磷肥（钙镁磷肥）的使用减缓了土壤pH值的降低。     

沼液与氮肥配施对小麦产量及品质的影响

为给小麦高产优质生产中沼液利用提供依据,在大田条件下,研究了沼液与氮肥配施对小麦籽粒产量及品质的影响.结果表明,在全生育期总施氮量240 kg·hm-2条件下,在基施化肥氮基础上追施沼液,尤其基施75％化肥氮和追施25％沼液氮配合处理(3/4U+1/4B)显著提高了淀粉糊化特性参数(峰值黏度、最终黏度、稀懈值和反弹值)、粉质参数(形成时间、稳定时间、粉质指数)和拉伸参数(拉伸面积、拉伸阻力和拉伸比).籽粒蛋白组分(总蛋白、球蛋白、醇溶蛋白和谷蛋白)含量以基施50％化肥氮和追施50％沼液氮配合处理(1/2U+1/2B)最高,3/4U+1/4B处理次之.籽粒蛋白组分、粉质与拉伸参数均以单施沼液处理(1/2B+1/2B)最低,单施化肥氮的处理(1/2U+1/2U)次之,而淀粉糊化特性参数以1/2U+1/2U处理最差,施用沼液的处理较1/2U+1/2U处理提高2.0％～6.5％.与1/2U十1/2U处理相比,基施沼液与追施化肥氮配合处理产量提高,而基施化肥氮与追施沼液氮处理的产量降低.可见在基施沼液的基础上追施化肥氮可提高小麦产量,其中1/2B+1/2U处理能够高产和优质兼顾,单施沼液效果低下.     

Temporal Growth Inhibition of Rice Plant and Growth Recovery Observed under Application of Anaerobically-DigestedCattle Manure

Abstract

A field experiment assessing the effect of the annual application of anaerobically-digested cattle manure (ADM), produced at a biogas plant, on paddy rice was conducted. In plots with ADM (MF), the early growth of rice plants, from transplanting to the active tillering stage, was inhibited compared to the plots with chemical fertilizers (CF). This phenomenon was observed over many growing seasons and was especially obvious in nitrogen uptake and leaf area index (LAI). However, after panicle initiation, the growth of MF-treated plants was equal to or superior to CF-treated plants. The grain yield in all the MF plots was 96 – 105% of that in the CF plots. The inorganic nitrogen content of the soil in the MF plots was higher than that in the CF plots, which was contradictory to the growth inhibition observed in the initial growth of plants in the MF plots. In contrast, the oxidation/reduction potential and pH of the surface soil in MF plots were within the normal range, indicating that these soil factors were not associated with growth inhibition observed in MF plots. Our results implied that rice cultivars with a long growing period that are able to recover from the initial growth inhibition, such as medium or late maturing cultivars, are suitable for paddy rice production fertilized with ADM.