中国野生大豆（G.soja）脂肪含量的多样性及地理分布

根据原产中国各地５１４７份野生大豆种子的脂肪含理分析结果，发现：１．脂肪含量与百粒重呈极显著正相关（ｒ＝０．９４０４＾＊＊＊）。２．４１６２份野生型（百粒重≤２．５ｇ）脂肪含量为９．２３％，５４２份半野生Ⅰ型（百粒重２．５１－５ｇ）为１３．０１％，４４３份半野生Ⅱ型（百粒重＞５ｇ）为１５．２３％。３．野生型脂肪含量与原产地经度（°Ｅ）呈显著负相关（ｒ＝－０．８８６２＾＊＊＊），与原产地纬度呈单峰曲     

泾阳棉区棉花产量与光温因素的通径分析

分析表明，泾阳棉区皮棉单产与６月份的平均气温呈显著正相关（ｒ＝０．７４＊）；与６月份日照时数呈显著正相关（ｒ＝０．７８＊）；与８月份的平均气温呈极显著正相关（ｒ＝０．８４＊＊）；５～９月份的降雨对泾阳棉区棉花高产属不利因素。通径分析结果，８月份的平均气温对皮棉产量的直接影响最大（ｄｙ·ｘ２＝０．３４３９４２）；６月份的平均气温通过８月份的平均气温对皮棉产量产生的间接影响（ｄｙ·ｘ１ｘ２＝０．１４９３１４）和６月份的平均气温通过６月份的日照时数对皮棉产量产生的间接影响（ｄｙ·ｘ２ｘ３＝０．１４８０６５）相似，且都较大。     

小麦耐穗发芽的评价与选择

通过标准加湿处理、手工脱粒种子发芽情况及落粒数的测定，研究了白粒小麦耐穗发芽能力的选择效果。降雨模拟器在促使群体平均值向高耐性水平漂移中是有用的，但在防雨条件下较大的基因型×年份工作使得对单个基因型未能作出准确的估计。最准确的估计是利用落粒数（ｔ２＝８０．７％）和手工脱粒种子的发芽情况（ｔ２＝３８．４％）得出的结果，这里没有基因型×年份的互作。种子休眠与降雨器中处理３天的落粒数的变化显著相关（ｒ＝－０．５６）。在１９９２年的模拟降雨试验中，穗发芽指数与种子休眠试验指数不相关（ｒ＝０．２０），但在１９９３年的发芽试验中，这两个指数间则为强相关（ｒ＝０．７３）。在相同试验各年份的比较中，落粒数（未作降雨处理）和种子休眠指数两年间的相关系数分别为ｒ＝０．６８和ｒ＝０．９，而两年间的穗发芽指数无相关（ｒ＝－０．０３）。     

甜菜对土壤肥力及酶活性影响的初步分析

研究结果表明，在被测定的１２种甜菜土壤养分含量中，有１０种养分含量低于对照（麦地）．其中，碱解氮，有效镁和有效锰的差异较大，其差值分别为２６ｐｐｍ、１１．５ｐｐｍ和７．４ｐｐｍ，其余差异不大；在甜菜营养生长期内，分别测定了甜菜与对照土壤的微生物总量、细菌、放线菌和真菌的数量及消长动态；土壤碱解氮含量和耕层地温对细菌数量有一定的影响；甜菜土壤的３项酶活性均低于同期的对照。在甜菜吸收氮素的高峰期，土壤中脲酶活性亦明显增强，达到１．２２ＮＨ＿３－Ｎｍｇ／ｇ土。在幼苗期至糖分积累期，磷酸酶活性与甜菜苗龄呈正相关关系（ｒ＝０．９２２３￣＊）。     

光籽对陆地棉经济性状影响的初步研究

初步研究表明，具有相同遗传背景的无短绒陆地棉光籽（包括其杂种分离类型，与正常有短绒棉籽相比，籽棉减产８．０５％～１８．５５％．皮棉减产２０．９２～２２．５１％．光籽与皮棉产量呈高度负相关。（ｒ＝－０．７３６４）．与表分呈显著负相关．（ｒ＝－０．９６４２）；与铃重呈高度负相关（ｒ＝－０．７０９２）；与纤维细度。断裂长度、生育期均呈中、高度负相关（ｒ分别为－０．７９６３、＝０．６７２５、＝０．７７８６）。籽致使品种纤维产量和品质迅速下降，是品种退化的重要原因之一。     

高产与高蛋白结合的小麦选择指标

本研究目的是确定春小麦改良高产与高蛋白的选择指标。选用的８个品种中，６个表达为对照水平，２个品种ＵＭ６３２和ＵＭ６８４表达为通常高于对照的水平性状。试验２年中，不同重复的植株从花期至成熟进行取样测定，收集有关Ｎ、干物质积累及重新代谢的资料。籽粒蛋白含量与籽粒产量不相关，但与花后Ｎ吸收（ｒ＝０．６６）、成熟期总植株Ｎ（ｒ＝０．４９）及Ｎ收获指数（ｒ＝０．８７）呈正相关，与成熟期营养器官Ｎ呈负相关（ｒ＝—０．４９）。籽粒产量与花后Ｎ（ｒ＝０．５０）和干物质量（ｒ＝０．７７）以及收获指数（ｒ＝０．８３）呈正相关。籽粒蛋白产量与花后Ｎ积累（ｒ＝０．９０）、成熟期总Ｎ（ｒ＝０．９８）、Ｎ收获指数及经济指数（ｒ＝０．９３）呈高度相关。ＵＭ６３２和ＵＭ６８４将花后Ｎ吸收和成熟期总Ｎ量高与高度代谢效率及Ｎ收获指数结合在一起，故此可以得出结论：遵循构建成熟期总Ｎ量高、Ｎ收获指数及经济指数高的杂交组合，通过群体中选择籽粒蛋白产量可以逐步改良具有高产和高蛋白含量的品种。     

不同施氮水平对甜菜地和休闲地土壤微生物数量的影响

本文比较了不同施氮水平对甜菜种植地和休闲地土壤中好气性细菌、放线菌和真菌数量的影响，并对土壤中的硝化细菌进行了测定。试验表明，氮肥能增加土壤中好气性细菌和硝化细菌的数量，并显著增加３０～６０ｃｍ土层中亚硝酸细菌数量，对５～１０ｃｍ、１０～３０ｃｍ和３０～６０ｃｍ土层中的硝酸细菌表现出促进作用。各施氮水平的土壤微生物总数达１×１０￣８个／ｇ干土以上。其中细菌为主要类群，约占９０％以上，其次为放线菌和真菌。在甜菜定苗前和收获期，５～３０ｃｍ土层中微生物总数顺序为施用Ｎ＿（１２０）＞Ｎ＿（１８０）＞Ｎ＿０，与甜菜的产量呈极显著正相关（ｒ＝０．９２８）．     

啤麦条纹病药剂防治试验研究

通过药剂筛选和药剂剂量选择试验研究。１５％多效唑可湿性粉剂按种子量２‰拌种；５０％粉锈宁可湿性粉剂按种子量０．３‰拌种，８０％４０２乳油按种子量０．５‰拌种；预防啤麦条纹病相对防效分别为１００％、１００％、９７．３％。无药害，有促进出苗作用，用药剂量小，预防效果极显著，经济有效。     

青薯168的生长发育及性状间的相关性分析

马铃薯的生长发育随着生育期的变化而变化,前期以茎叶生长为主,后期则以块茎生长为主。所以,地上部性状间,以及地上部性状与地下部性状—块茎的形成有着密切的关系。地上部株高与茎叶重、叶面积之间有着极显著的正相关,相关系数分别为ｒ＝０．９３５９＊＊,和ｒ＝０．８４８８＊＊;茎叶重与叶面积系数之间呈极显著的正相关,ｒ＝０．９６０５＊＊。株高与地下部块重之间呈显著的正相关,ｒ＝０．６８４３＊,茎叶重与地下部块重间的关系为正相关,ｒ＝０．４２８５,叶面积系数与地下部块重有着微弱的正相关关系,ｒ＝０．１５３４。     

地理环境对大豆种子中异黄酮积累的影响趋势

以７个异黄酮含量显著不同的大豆品种或品系分别在７个地区种植，利用ＨＰＬＣ技术分析所收种子中的异黄酮含量。以期获得种子中的异黄酮积累与地理环境之间的相互关系。结果显示，在７个地区中各品种异黄酮平均含量显著不同，山西最高（２４９２．３μｇ／ｇ），湖北最低（７６３．４μｇ／ｇ）。同一品种在不同地区其变异系数也不同，吉林３号变异系数最高（４９．４％）；张家口黑豆最低（３３．５％）。７个大豆品种异黄酮含量与纬度和海拔高度呈正相关，与经度呈负相关，且与纬度的相关程度最高，均达到显著或极显著水平，与经度和海拔高度的相关程度相对较低。另外，各品种与各地区的平均气温和降水量呈负相关，与日照时数呈正相关，且与平均气温的相关程度最高（ｒ＝－０．８７９＊＊）     

Studies on natural repellents against potato tuber moth (Phthorimaea operculella Zeller) in country stores

Summary The effects of the leaves of five plant species, one from each of the generaAmbrosia, Anemone, Eupatorium, Eucalyptus andLantana, on potato tuber moth were investigated under indigenous storage conditions at the Central Potato Research Station, Shillong (1800 m above sea level). Their action was compared with that of a biological insecticide (spores ofBacillus thuringiensis), a chemical insecticide (carbaryl), and an untreated control. The data collected after six months storage on tuber damage, sprout damage and the rotting indicated that the leaves ofLantana aculeata provided most protection to the tubers, reducing damage from over 70% in the check to below 5%, and sprout damage from over 45% to below 3%. Next best wasEucalyptus globulus followed byB. thuringiensis. They may be used on tubers stored for table use or for seed as they had no adverse effect on germination or on the yield of a subsequent crop.     

Comparative Assessment of the Efficacy of Various Nitrogen Fertilizers

Summary

The efficiency of N fertilizers is usually poor; often less than 50% of the applied N is taken up by the crop. This review focuses on various N fertilizers with respect to the significance of different N loss pathways, namely (i) ammonia volatilization, (ii) dinitrogen and nitrogen oxide emissions, and (iii) nitrate leaching. Further, the significance of biological N immobilization, ammonium fixation and, finally, the impact of nitrate vs. ammonium uptake on crop yield are also discussed. The reviewed literature shows that N fertilizers may differ markedly in their susceptibility to losses. There is, however, considerable scope to improve N efficiency of each N source by proper N management practices.     

In vitro antimicrobial activity of different accessions ofSolanum commersonii Dun. from Uruguay

Summary The in vitro antimicrobial activity of extracts from accessions ofSolanum commersonii Dun. collected in the south of Uruguay was investigated against five microorganisms including the pathogenRalstonia solanacearum. A total of 30 extracts corresponding to organic and aqueous extracts were studied. Interestingly, most of the positive results for growth inhibition were againstR. solanacearum. The extracts were also analyzed for the presence of glycoalkaloids and lectins. Six of the ten aqueous extracts showed lectin presence and a wide variation in the type and amounts of glycoalkaloids, was found. Results indicate that there is no clear relationship between the antimicrobial activity against the five microorganisms screened and the presence or amounts of lectins and glycoalkaloids, traditionally regarded as possible antimicrobial metabolites in theSolanum genus, which indicates the presence of as yet unidentified antimicrobial compounds.     

Pharmacokinetics of Marine-Derived Drugs

Marine organisms represent an excellent source of innovative compounds that have the potential for the development of new drugs. The pharmacokinetics of marine drugs has attracted increasing interest in recent decades due to its effective and potential contribution to the selection of rational dosage recommendations and the optimal use of the therapeutic arsenal. In general, pharmacokinetics studies how drugs change after administration via the processes of absorption, distribution, metabolism, and excretion (ADME). This review provides a summary of the pharmacokinetics studies of marine-derived active compounds, with a particular focus on their ADME. The pharmacokinetics of compounds derived from algae, crustaceans, sea cucumber, fungus, sea urchins, sponges, mollusks, tunicate, and bryozoan is discussed, and the pharmacokinetics data in human experiments are analyzed. In-depth characterization using pharmacokinetics is useful for obtaining information for understanding the molecular basis of pharmacological activity, for correct doses and treatment schemes selection, and for more effective drug application. Thus, an increase in pharmacokinetic research on marine-derived compounds is expected in the near future.     

Novel food and non-food uses for sorghum and millets

Sorghum and millets have considerable potential in foods and beverages. As they are gluten-free they are suitable for coeliacs. Sorghum is also a potentially important source of nutraceuticals such antioxidant phenolics and cholesterol-lowering waxes. Cakes, cookies, pasta, a parboiled rice-like product and snack foods have been successfully produced from sorghum and, in some cases, millets. Wheat-free sorghum or millet bread remains the main challenge. Additives such as native and pre-gelatinised starches, hydrocolloids, fat, egg and rye pentosans improve bread quality. However, specific volumes are lower than those for wheat bread or gluten-free breads based on pure starches, and in many cases, breads tend to stale faster. Lager and stout beers with sorghum are brewed commercially. Sorghum's high-starch gelatinisation temperature and low beta-amylase activity remain problems with regard to complete substitution of barley malt with sorghum malt . The role of the sorghum endosperm matrix protein and cell wall components in limiting extract is a research focus. Brewing with millets is still at an experimental stage. Sorghum could be important for bioethanol and other bio-industrial products. Bioethanol research has focused on improving the economics of the process through cultivar selection, method development for low-quality grain and pre-processing to recover valuable by-products. Potential by-products such as the kafirin prolamin proteins and the pericarp wax have potential as bioplastic films and coatings for foods, primarily due to their hydrophobicity.     

La pourriture fongique des tubercules de pomme de terre en atmosphère contrôlée

Résumé Fusarium culmorum et, dans une moindre mesure,F. avenaceum, F. sambucinum, Colletotrichum coccodes etCylindrocarpon sp. ont été isolés de tubercules entreposés en atmosphère contr?lée (AC) et atteints de pourriture grave. Des expériences d'inoculation de tubercules avec 10 espèces ou variétés fongiques et conservation en AC à 8 et 10°C ont montré que: 1) les champignons non pathogènes en atmosphère normale (ex.:Cylindrocarpon sp.) le devenaient en AC, 2) ceux qui étaient faiblement pathogènes en atmosphère normale étaient nettement plus virulents en AC (ex.:F. culmorum), 3) les espèces ou variétés virulentes en conditions d'air normales (Fusarium sulphureum etPhoma exigua var.foveata) ne provoquaient pas de pourriture plus grave en AC et leur activité pouvait même être légérement freinée.

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Summary The extensive rotting of tubers which occurred during storage trials in controlled atmospheres has led to a mycological and pathological study of the rots. The most frequently isolated species wasFusarium culmorum althoughF. avenaceum, F. Sambucinum, Coletotrichum coccodes and aCylindrocarpon sp. were also present. Tubers of cvs Bintje and Ulla were inoculated with 19 isolates of 10 fungal species or varieties associated with rotting of stored tubers (Table 1). The tubers were incubated at 8 or 10°C in normal or controlled (2% O₂:1% CO₂, 2% O₂:6% CO₂, 2% O₂:12% CO₂, 6% O₂:6% CO₂) atmospheres and the extent of rotting was determined a month later (Tables 2 and 3 and Fig. 2). Species which were weakly virulent in a normal atmosphere (variousFusarium spp. andPhoma exigua var.exigua) were clearly more aggressive in the controlled atmosphere and this was especially evident in the case ofF. culmorum. Species ot pathogenic in a normal atmosphere under experimental conditions (Colletotrichum coccodes, Cylindrocarpon sp.,Phoma eupyrena) caused rotting in the controlled atmosphere. The two species which were virulent in a normal atmosphere (F. sulphureum andP. exigua var.foveata) were also virulent in the controlled atmosphere and in some cases the extent of rotting was even less. As a general rule, rotting caused by weakly virulent fungi was greater in both cultivars in the controlled than in a normal atmosphere.

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Zusammenfassung Eine starke F?ulnis von in Kontrotrollierter Atmosph?re gelagerten Kartoffelknollen veranlasste eine mykologische und pathologische Untersuchung. Der am h?ufigsten isolierte Pilz warFusarium culmorum. F. avenaceum, F. sambucinum, Colletotrichum coccodes undCylindrocarpon sp. wurden ebenfalls bestimmt. Neunzehn St?mme von 10 Pilzsorten oder-Arten welche bei Lagerf?ulnis vorkommen (Tab. 1) wurden auf Knollen der Sorten Bintje und Ulla inokuliert. Die Lagerung wurde bei 8 oder 10°C under Normalbedingungen oder kontrollierter Atmosph?re durchgeführt (2% O₂:1% CO₂, 2% O₂:6% CO₂, 2% O₂:12% CO₂, 6% O₂:6% CO₂). Der F?ulnisgrad (Abb. 1) wurde nach einem Monat bonitiert. Die Ergebnisse sind in den Tabellen 2 und 3 dargestellt und in der Abb. 2 illustriert. Unter in Normalbedingungen nur schwach virulente Arten (verschiedeneFusarien undPhoma exigua var. exigua) waren deutlich aktiver in kontrollierter Atmosph?re. Dies was besonders der Fall fürF. culmorum. Pilze, die im Versuch unter Normalbedingungen nicht pathogen waren (Colletotrichum coccodes, Cylindrocarpon sp., Phoma eupyrena), haben unter kontrollierter Atmosph?re Sch?den verursacht. Hingegen werden die beiden unter Normalbedingungen sehr pathogenen Arten (F. sulphureum undP., exigua var.foveata), durch kontrollierte Atmosph?re wenig beeinflusst. Es wurde sogar in einigen F?llen eine gewisse Verringerung des F?ulnisbefalls beobachtet. Ganz allgemein haben die getesteten kontrollierten Bedingungen die Entwicklung der Pilzf?ulnis an beiden Kartoffelsorten gef?rdert, speziell durch Pilzarten, welche unter Normal-bedingungen wenig Sch?den verursachen.

     

Extreme resistance to infection by potato virus X in genotypes of wild tuber-bearingSolanum species

Summary Clones derived from thirty-one different accessions (nineteen of Argentine origin) belonging to eightSolanum species were screened for resistance to infection by potato virus X strain cp (PVX cp) by mechanical inoculation of plantlets that had been micropropagated in vitro. Estimates of PVX multiplication obtained by enzyme linked immunosorbent assay and slot blot nucleic acid hybridization allowed the identification of resistant clones derived from five accessions belonging toS. commersonii S. oplocense, S. sparsipilum andS. tuberosum andigena. Resistant genotypes supported PVX concentrations 5 to 15 times smaller than did the susceptible control cultivar Spunta. Graft inoculation test confirmed the presence of extreme resistance similar to that conferred by the ‘immunity’ gene X¹ (also called RX_act).     

Marine Pharmacology in 2009–2011: Marine Compounds with Antibacterial,Antidiabetic, Antifungal,Anti-Inflammatory,Antiprotozoal, Antituberculosis,and Antiviral Activities; Affecting the Immune and Nervous Systems,and other Miscellaneous Mechanisms of Action ?

The peer-reviewed marine pharmacology literature from 2009 to 2011 is presented in this review, following the format used in the 1998–2008 reviews of this series. The pharmacology of structurally-characterized compounds isolated from marine animals, algae, fungi and bacteria is discussed in a comprehensive manner. Antibacterial, antifungal, antiprotozoal, antituberculosis, and antiviral pharmacological activities were reported for 102 marine natural products. Additionally, 60 marine compounds were observed to affect the immune and nervous system as well as possess antidiabetic and anti-inflammatory effects. Finally, 68 marine metabolites were shown to interact with a variety of receptors and molecular targets, and thus will probably contribute to multiple pharmacological classes upon further mechanism of action studies. Marine pharmacology during 2009–2011 remained a global enterprise, with researchers from 35 countries, and the United States, contributing to the preclinical pharmacology of 262 marine compounds which are part of the preclinical pharmaceutical pipeline. Continued pharmacological research with marine natural products will contribute to enhance the marine pharmaceutical clinical pipeline, which in 2013 consisted of 17 marine natural products, analogs or derivatives targeting a limited number of disease categories.