Molecular differentiation,diversity, and folk classification of “sweet” and “bitter” cassava (<Emphasis Type="Italic">Manihot esculenta</Emphasis>) in Caiçara and Caboclo management systems (Brazil)

This study focuses the inter-relationships among the men, the use, and the intra-specific cassava diversity, under the perspective of this crop evolutionary dynamics. The origin, the use and the current local management of varieties with high and low cyanogenic potential are important questions around cassava domestication. We collected 169 local varieties identified as “sweet” or “bitter” cassava by traditional farmers from Atlantic Forest and Amazon (Medium Negro River Basin), Brazil. Using a population genetics and an ethnobotany approach, the diversity and the genetic structure of cassava were evaluated. We found a total of 115 vernacular names, and in the Atlantic Forest sample the average genetic diversity (H _S = 0.654) was higher for the sweet varieties than for bitter ones (0.582). The genetic differentiation coefficient (R _ST), used to estimate the diversity among groups, was 0.057 (P < 0.001), indicating that the divergence between the two groups is low. We obtained a low correlation between the morphological and genetic distances, and the congruence was high when the ethnoclassification and the genetic structure were considered. We discuss the adaptive advantages of the sweet varieties use, the current socio-economic changes in bitter varieties use, and the ecological history of these variety groups.     

Collecting maize (<Emphasis Type="Italic">Zea mays</Emphasis> L. convar. <Emphasis Type="Italic">mays</Emphasis>) with potential technological ability for bread making in Portugal

The Portuguese maize bread (“broa”) manufactured from traditional maize landraces still plays an important economic and social role on Central and Northern rural communities of the country. However the traditional maize landraces agricultural systems are changing. Local maize landraces are in risk of disappearing because of the progressive adoption of hybrid varieties not suitable for bread production. These changes are contributing to a major loss of genetic diversity. An expedition took place in the Central region of Portugal (Beira Interior and Beira Litoral) with the purpose of collecting enduring maize landraces with technological ability for bread production and to access the possibility of establishing a participatory plant breeding and conservation program. A total of 51 different maize landraces and 175 other varieties of associated crops were collected. Maize landraces showed to maintain high diversity and potential for improvement. The production relayed on small farms with polycrop, quality oriented, sustainable systems. A participatory plant breeding and conservation program would be possible on this region with the proviso that local authorities would be involved. This program would allow a higher valuation of these maize populations, contributing to halt the current loss of these unique Portuguese maize landraces.     

间套混种控病增产机制研究进展

间套混种是一种能够提高农田生物多样性的种植方式,具有充分利用资源和大幅度增加产量的特点,也是利用种间互作控制病害发生的传统农作措施。间套混种在解决人口不断增长、资源日益枯竭和生态环境恶化等问题方面具有重要的现实意义,已经成为近年来国内外研究的热点。间套混作在一起的作物具有非常复杂的相互作用关系,这种相互作用关系涉及地上部(冠层)的相互作用和地下部(根际)的相互作用,作物对水分、养分和光热等资源的高效利用、复合群体产量优势和病害持续控制都是与种间相互作用相联系的。本文综述了间套混种系统中地上部因素和地下部因素在作物病害控制和产量优势中的作用效果及机制,并展望了当前研究重点及今后需要研究的方向。     

Genetic diversity in Tunisian perennial ryegrass revealed by ISSR markers

The genetic diversity in Tunisian perennial ryegrass (Lolium perenne) was examined by the help of inter-simple sequence repeats (ISSR). Starting from eighteen accessions, a large number of polymorphic ISSR markers were currently generated using appropriate primers (a total of 136, which average of 12.6 polymorphic bands/primer). These markers were considered to estimate the genetic distance among accessions and to draw phylogenetic trees. Our data provide evidence of a high degree of genetic diversity in Tunisian ryegrass. In addition, both cultivars and wild types present a high degree of divergence suggesting a complex domestication process in this crop. Moreover, spontaneous populations of Tunisian ryegrass have been identified as important ecotypes that are suitable in selection programs to improve grasslands.     

Soil acidity under multiple land-uses: assessment of perceived causes and indicators,and nutrient dynamics in small-holders’ mixed-farming system of northwest Ethiopia

Knowledge on farmers’ perspectives is of paramount importance in order to design appropriate agricultural research and development interventions attuned to local farming systems. A participatory rural appraisal was conducted in order to understand perceived causes and indicators of soil acidity under multiple land-uses in three Districts of northwest Ethiopia. Soil samples were also collected from five dominant land-uses. The samples were analysed for soil pH, exchangeable acidity and other physico-chemical properties. The result indicated that the pH_(H2O) of most of the soils in the study sites were in a strongly acidic range (4.6–5.5). Gashena Akayita of the Banja District was the most acidic of all. Among the land-uses, eucalyptus fields were the most acidic followed by crop outfields and grazing lands in that order. At all the study sites, exchangeable Al was detected in soils having a pH of less than 5.0. Overall, the nutrient dynamics showed variation across land-uses and study sites. Farmers’ perceived causes of soil acidity included: soil erosion; contending use of fertility replenishing local resources; abandoning traditional fertility management practices and minimal use of external inputs. The farmers attributed the exclusive use of acid-forming inorganic fertilizers to exhaustion of the soil. Various land and soil characteristics, plant growth attributes, changes in genetic diversity were mentioned as indicators of soil acidity. Particularly, the farmers used prevalence of acidophilic weed species on crop fields and grazing lands as marker of strongly acidic soil. Farmers’ perceived causes and indicators were in agreement with scientific facts and can be utilized as input in designing sustainable acid soil management strategies. Decline in genetic diversity of the once widespread crop species and land races, and expansion of newly introduced soil acidity tolerant species, suggests the need to undertake rescue collections in these areas.     

气候变化下黄土高原耕作系统演变与适应性管理

耕作系统主要包括土壤系统、作物系统和区域气候系统等几个相对独立、但又紧密关联的组成部分,涉及作物栽培模式、作物类型、杂草和病虫害及农田水土资源管理等方面,在黄土高原生态系统管理和农业可持续发展中占有重要地位。过去50a (1951-2000年),黄土高原的年平均气温升高了1.1℃,且其变率逐渐增加,降雨和热量资源分布呈现复杂的时空异质性。作物种植区域的变迁、熟制制度的演变和农田灾害的加剧促使农田管理模式不断寻求改变,对当地农业耕作系统产生了深远影响。本文总结了黄土高原过去多年的气候变化（气温、降水量、积温）特征和发展趋势,气候变化下耕作系统（种植区、耕作制度、土壤环境）和作物系统（需水量、物候、品种、产量）的演变规律,作物与土壤互作关系,以及气象灾害对黄土高原耕作系统的影响,并提出气候变化下耕作系统适应性管理途径和策略。旨在为黄土高原耕作技术和田间管理提供新的理论,寻求气候变化下区域农业可持续发展应对策略。     

The diversity and sustainable development of crop genetic resources in the Lancang River Valley

This paper reviews the status of crop genetic resources in Yunnan province of China from 1978 to 1999. Results are presented of same research in the field of the diversity of cultivated and wild crop. Yunnan is one of the centre of origin or genetic diversity of more than 200 cultivated and wild crops. There are over 500 cultivated plants which account for over 80% of the total in China and more than 650 species of wild crops. In addition, there are more than 440 species of main wild flowers. According to our recently researches there are abundant species, subspecies and varieties of crop genetic resources in Yunnan Province. The Lancang River Valley is the richest genetic diversity centre of rice, maize, wheat, barley, buckwheat, legumes, ramie, sugarcane, vegetable, tea, actinidia and so on. For example, there are 59 varieties (including all varieties of Oryza sativa L. in China) in 5933 accession of Yunnan indigenous rice. The Lancang River Valley is one of the centre for genetic diversity of rice resources and a rich region for elite and rare rice resources of Yunnan, too. In order to protect the highly endangered crop genetic resources in the Lancang River Valley, it is necessary and very important to set up a collection, conservation, utilization and research system, enhancing their protection and utilization, in situ- and ex situ-conservation, farm management and sustainable production.     

Genetic structure of quinoa (<Emphasis Type="Italic">Chenopodium quinoa</Emphasis> Willd.) from the Bolivian altiplano as revealed by RAPD markers

Quinoa (Chenopodium quinoa Willd.) is a pseudocereal originated from the Andes important for small farmers’ food security as well as for commercial production. Recently, it has been claimed that in Bolivia genetic erosion could result from the marginalization of the crop in the north and from its commercial standardization in the south. The aim of this study was to quantify the hierarchical structure of the genetic variation present in eight quinoa field populations, consisting of cultivated and weedy individuals, representative of the altiplano and interandean valleys of Bolivia. Randomly amplified polymorphic DNA markers show that quinoa has a strong population structure and a high intra-population variation. An effect of geographical structure of the populations was highlighted, due to population isolation, not simply linked to distance but more probably to climatic and orographic barriers present in the studied zone. The population structure is also reinforced by the limited seed exchanges among farmers as revealed by field interviews. This population structure appears related to three major biogeographic zones: the northern and central altiplano, the interandean valley, and the southern Salar. Intrapopulation genetic diversity was higher than that expected for a mainly autogamous species, and higher than that reported in anterior studies based on germplasm collections. These results are commented in view of current knowledge on phylogeny and reproductive biology of the species, and their implications regarding genetic resources management are discussed.     

Spatial Distribution of Genetic Diversity in Wild Populations of Phaseolus vulgarisL. from Guanajuato and Michoacán, Méexico

The diversity, genetic structure, and genetic flow of wild populations of Phaseolus vulgaris L. within its Mesoamerican area of domestication, were analyzed by means of morphological and inter-simple sequence repeat molecular markers. Overall, 89% of the loci studied were polymorphic, 35% in the least diverse population and 65% in the most diverse. Genetic diversity in the populations was high, between h = 0.14 and 0.29, as was the maximum distance between populations (D = 0.3). Between 40% and 45% of the diversity was explained by the differences among populations, indicating that a large number of populations is necessary to represent the wild gene pool in the germplasm collections. We found uniformity in allele frequencies among the populations, suggesting presence of outcrossing. We did not find correlation between genetic and geographic distances, but the dendrogram topology suggests geographical isolation due to the mountainous topography. Negative correlations were observed between the coefficient of variation of seed size and the distance between wild populations and fields . We obtained a highly negative correlation between percentage of polymorphic loci and distance to the nearest crop field, which also suggests gene flow from the domesticated populations. These observations suggest that genetic flow is taking place from domesticated toward wild populations and that the farmer, through his agricultural activities, could be influencing the magnitude and the characteristics of the gene flow, and along with this, the differentiation of wild populations. New approaches should be established for conservation in situ and maintaining bio-safety, given the risk of introducing genotypes from the Andes and transgenic varieties and causing genetic assimilation.     

Centres of Crop Diversity and/or Origin, Genetically Modified Crops and Implications for Plant Genetic Resources Conservation

The concept of centres of crop diversity and/or origin of agriculture is briefly reviewed. The conservation status of crop genetic resources, either ex situ or in situ, cultivated or wild, has been assessed for species of the Central American and Mexican centre, demonstrating that that region is indeed one of the important centres of crop diversity for human kind. Furthermore, biotechnological developments with regard to the creation and spread of genetically modified crops have been analyzed. The likelihood of unintentional introgression of genetically modified traits into conventional seed lots, crops as well as into germplasm collections have been assessed. Related biosafety measures as well as the possible implications of intellectual property rights on transgenic crops and/or genes are being discussed vis-a-vis the possible implications they might have for germplasm management. The Central American crop genetic resources situation has been used as a “case study” to illustrate the potential impact of the spreading of GM varieties on the genetic diversity in genebanks and farmers’ fields and the need for effective and efficient conservation efforts. Conservation management strategies and practices are being proposed of mitigate the potential negative impact of GM crops on the conservation efforts.     

Inter-Simple Sequence Repeat fingerprints to assess genetic diversity in Tunisian fig (Ficus carica L.) germplasm

The genetic diversity of 18 Tunisian fig cultivars was investigated at the DNA level using the Inter Simple Sequence Repeat (ISSR) associated with the Polymerase Chain Reaction (PCR). Using a set of primers, the most informative ones were selected that were characterized by an important Resolving power value of 29.6. A total of 47 discernible fragments were scored from samples, with a mean of 11.7 fragments per primer. The 90.4% of sample that were polymorphic were scored as molecular markers to examine the Tunisian fig germplasm polymorphism at DNA level. A large genetic diversity as related to ISSR patterns was found within the local Tunisian fig germplasm. An UPGMA dendrogram exhibits the unstructured variability in this crop. Moreover, the principal component analysis shows that the observed diversity was typically continuous. Our data provide a large number of ISSR markers that are useful in the fingerprinting of Ficus carica L. cultivars, and in the understanding of the genetic relationships among these accessions.     

Spatial and temporal dynamics in genetic diversity in upland rice and late millet (<Emphasis Type="Italic">Pennisetum glaucum</Emphasis> (L.) R. Br.) in The Gambia

Ambiguity exists about the level of genetic diversity represented by farmer crop varieties, how it develops over time and how it relates to the diversity comprised by formal varieties. As part of an interdisciplinary technological/sociological study on farmer management of gene flow, upland rice (Oryza sativa L.) and late millet (Pennisetum glaucum (L.) R.Br.) from The Gambia were investigated for morphological and molecular variation. The goal of these analyses was to obtain insight into the level of crop genetic diversity of farmer’s materials planted in several case study villages in The Gambia. For both crops, samples were collected from villages and various research institutes. Based on variety names, different rice and millet varieties were expected to be used in different villages. In fact, there was a large overlap in genetic diversity for both crops, masked by the use of synonyms. The considerable similarity in rice genetic diversity between villages most likely results from the exchange of varieties between farmers. For millet this seems the result of development of varieties from the same gene pool. Some farmer varieties of rice, however, are apparent hybrid forms between the species O. sativa and O. glaberrima Steud., and farmer varieties in general displayed higher levels of genetic diversity than formal varieties. This indicates that, for rice, genetic diversity develops in farmers’ fields and may have potential use in formal breeding programs.     

Biodiversity in Ethiopian linseed (<Emphasis Type="Italic">Linum usitatissimum</Emphasis> L.): molecular characterization of landraces and some wild species

Molecular characterization of germplasm is important for sustainable exploitation of crops. DNA diversity was measured using inter-retrotransposon-amplified-polymorphism and inter-simple-sequence-repeat markers in 203 Ethiopian landraces and reference varieties of linseed (flax, Linum usitatissimum) and wild Linum species. Molecular diversity was high (PIC, 0.16; GD, 0.19) compared to other reports from the species. Genotyping separated reference from landrace accessions, and clustered landrace accessions from different altitudes and geographical regions. Collections showed evidence for recent introduction of varieties in some regions. The phylogeny supported L. bienne Mill. as the progenitor of domesticated L. usitatissimum. Markers developed here will be useful for genetic mapping and selection of breeding lines. The results show the range of characters that can be exploited in breeding lines appropriate for smallholder and commercial farmers in Ethiopia, producing a sustainable, secure, high-value crop meeting agricultural, economic and cultural needs.     

Plant Growth Retardants,Plant Nutrients,and Cotton Production

Soil fertility and crop management are the two most important factors of modern agricultural activity. Managing the balance of vegetative and reproductive growth is the essence of managing a cotton crop. It is well known from numerous fertilizer experiments that the yield of agricultural crops has been strongly dependent on the supply of mineral nutrients, which have been used in crop cultivation to exploit the full genetic potential of the plant. Supplying optimal quantities of mineral nutrients to growing crop plants is one way to improve crop yields. In recent years, several approaches have been tried to break this yield plateau, and among them is application of plant growth regulators (PGRs). In maximizing the quantity and quality of a crop it is necessary to identify the constraints that may affect it and to devise methods of overcoming them through the use of inputs or changes in management practices.     

覆盖作物的效益及其合理选择的研究进展

覆盖作物的种植是一种可实现农业可持续发展的保护性耕作措施，对维护农业生产的可持续性具有积极意义。目前，对覆盖作物的研究主要侧重于覆盖作物对土壤和后茬作物的影响评价，包括对土壤有机碳、氮的固存，对杂草和病虫害的抑制、对土壤理化性质的改良和对土壤微生物活性的影响等方面。然而，覆盖作物的效益因覆盖作物类型、地理位置和覆盖时间不同而存在差异。另外，覆盖作物的选择、设置方法、终止方法、经济效益等充满了不确定性，这限制了其在农田中的大面积推广。该研究基于当前国内外的研究进展简要介绍了覆盖作物的种植管理现状，总结了覆盖作物提供的多种生态系统服务功能，讨论了限制覆盖作物在农作物种植系统中广泛应用的关键因素，提出了覆盖作物合理选择的建议。同时，基于覆盖作物在农学、生态环境上的经济和生态效益，对覆盖作物的贡献以及面临的瓶颈进行了思考。发现良种培育、覆盖作物-土壤微生物-土壤养分-作物之间的协同机制仍是今后需要突破的重点课题，以期为覆盖作物的合理选择及大面积推广提供依据。     

A Suitable Site for in situ (On-farm) Management of Plant Diversity in Traditional Agroecosystems of Western Himalaya in Uttaranchal State: A Case Study

There is a growing realisation world over that the introduction of modern agriculture has to be supplemented with measures to conserve biodiversity in situ if yield gains are to be stabilized. Hence, there is a growing interest from agricultural development specialists and conservation biologists for understanding the socioeconomic factors determining the conservation of biodiversity in situ. The present study was conducted with the objective of understanding the in situ (on-farm) conservation of agrobiodiversity in traditional agroecosystems taking the Urgam valley in north-western Himalaya of India, as a case study. An inventory was made of traditional crops and wild economic species for subsistence, and the structure of forest resource base, traditional knowledge related to resource management and use. Institutional and scientific challenges for in situ (on-farm) management of crop diversity were studied and are discussed in this paper. Complementarity of in situ (on-farm) conservation with ex situ conservation together with crop improvement in such marginal areas are suggested.     

Diachronic (1979–2003) analysis of rice genetic diversity in Guinea did not reveal genetic erosion

Greater insight into the dynamics of genetic resources of crop plants is needed in order to pinpoint detrimental evolutionary patterns and draw up conservation priorities. Temporal evolution of rice genetic diversity was monitored in Maritime Guinea where subsistence-oriented agriculture prevails. Diachronic comparison was performed between samples collected in six villages during the 1979/1982-period and in 2003, based on the names and number of varieties inventoried and the polymorphism of microsatellite markers. The number of varieties appeared not to be comparable between the two dates, due to differences in the collection methods. The varietal composition had evolved very substantially between the two collection dates. Many long-duration varieties present in 1979/1982 had been abandoned and several improved varieties had been introduced. The mean number of alleles per locus and per accession was significantly higher in accessions collected in 2003. Pairwise comparisons of the mean number of alleles per locus in 1979/1982–2003 homonymous accession pairs indicated higher intra-accession diversity for the 2003 collections. Genetic differentiation, measured with the F _ST values, was very high and significant for more than 80% of these pairs of accessions. The overall genetic differentiation between accessions from the two collections dates was also significant. Significant changes were also observed for allelic composition. However, alleles specific of each collection date had much lower frequency, compared to alleles common to the two collection dates. These results suggest that rice genetic diversity in Maritime Guinea has been maintained or even enhanced. Old collections of crop genetic resources are often not exhaustive enough to undertake perfect diachronic comparison. New methods to utilize this historical data for diversity monitoring are needed.     

Genetic diversity of potato landraces from northwestern Argentina assessed with simple sequence repeats (SSRs)

Andean potato varieties are cultivated in the northwest of Argentina and constitute the most important staple food for the local farmers. The genetic diversity of 155 accessions conserved at the Genebank of Balcarce (INTA) was tested using four microsatellites. Three commercial potato varieties of Tuberosum group and one accession of Curtilobum group were used as outgroups. The presence of bands was scored for each microsatellite and for each accession and the data were analysed by principal coordinate analysis. The polymorphism information content was obtained for each molecular marker from banding patterns. Analysis of molecular variance was carried out with a variable number of accessions for each landrace, from different departments and sites within departments. More than one genotype was detected in the majority of the potato landraces. Some accessions within each landrace did not differentiate. AMOVA revealed that most of the genetic variation occurred among sites within departments and among local varieties. These findings are discussed considering the agricultural practices carried out in the Andean farming system.