应用PMSG,PMSG＋HCG和氯前列烯醇提高母猪繁殖试验

应用ＰＭＳＧ、ＰＭＳＧ＋ＨＣＧ和氯前列烯醇处理临床上健康的繁殖适龄“长Ｘ大”杂种母猪９６头，１０ｄ内同期发情率分别为９８．１５％、９９．３３％和９０．２３％，均与对照组的４６．７０％间有极显著差异（Ｐ〈０．０５）；情期受胎率分虽为９８．０５％、１００％和１００％、与对照组间无显著差异（Ｐ〉０．０５）；胎平均产仔数分别为１３．１头、１５．３头和１５．８头，均与对照组间差异不显著，但试验组胎平均产仔数     

PMSG提高初产母猪繁殖力的研究

７０头初产断奶母猪随机分为两组，试验组于断奶当日颈部肌肉注射１０００Ⅰ．Ｕ．ＰＭＳＧ；对照组只注射等容量生理盐水（注射用水），以观察初产母猪断奶后发情配种和２胎产仔的情况。结果表明：试验组母猪断奶发情配种的时间比对照组显著提前（Ｐ＜０．０１）；２胎产仔数增加（Ｐ＜０．０１）；而两组母猪发情配种的受胎率差异不显著（Ｐ＞０．０５）。     

维生素ADE和孕马血清促性腺激素分别结合前列腺素对母黄牛同期发情和受胎效果的试验

应用孕马血清促性腺激素（ＰＭＳＧ） 和维生素ＡＤＥ（Ｖｉｔ．ＡＤＥ） 分别结合前列腺素（ＰＧ） 对１２９ 头母黄牛进行同期发情定时输精试验。试验分组比较了ＰＭＳＧ＋ ＰＧ、Ｖｉｔ．ＡＤＥ＋ ＰＧ 和ＰＧ 处理组母牛之间的同期发情率和受胎率。结果表明三个处理组中采用ＰＭＳＧ 结合ＰＧ 促使母黄牛同期发情效果最好,明显高于采用Ｖｉｔ－ＡＤＥ 结合ＰＧ（Ｐ＜０－０１） 和ＰＧ（Ｐ＜ ０－０５） 处理组;相对于ＰＧ 处理组,采用Ｖｉｔ－ＡＤＥ 结合ＰＧ 并没有提高母黄牛的同期发情率（Ｐ＞ ０－０５） ;三个试验组母牛的受胎率都比较低,没有显著差异（Ｐ＞ ０－０５） 。     

改进集约化条件下瘦肉型母猪繁殖力综合技术措施研究初报

本研究试验了（１）孕马血清促性腺激素（ＰＭＳＧ）诱导乏情后备母猪发情受胎的效果;（２）ＰＭＳＧ结合氯前列烯醇（ＰＧ）诱导乏情后备地和经产母猪发情受胎的效果;（３）环内沙星结合５％盐水和ＰＧ治疗母猪子宫炎症的效果。结果表明,单独使用ＰＭＳＧ诱导乏情后备母猪发情时发情率高,但受胎率不理想;ＰＭＳＧ结合ＰＧ诱导乏情的后备和经产母猪发情的效率好,发情后受胎率亦高;利用环丙沙星结合５％盐水和ＰＧ醇治疗母猪子     

三合激素对绵羊同期发情作用的研究

本试验采用三合激素（ＩＴＣ）为主的处理方案,对繁殖季节中１７９只母羊进行了同期发情处理。按５５ｋｇ以上体重的母羊第一次注ＩＴＣ２．０ｍｌ,间隔１４天,第二次注１．０ｍｌ;５５ｋｇ以下母羊第一次注１．５ｍｌ,第二次注０．８ｍｌ,于第二次注射同时随机分为６组。①ＰＭＳＧ１９０ＩＵ;②ＬＲＨ─Ａ＿２５０μｇ＋ＰＭＳＧ１５０ＩＵ;③ＬＲＨ－Ａ＿３５０μｇ＋ＰＭＳＧ１５０ＩＵ;④ＦＳＨ２０单位＋ＰＭＳＧ１５０ＩＵ;⑤ＬＨ５０单位＋ＰＭＳＧ１５０ＩＵ;⑥ＰＭＳＧ５００ＩＵ或ＬＲＨ－Ａ＿３１００μｇ。试验结果表明,第一次注射和第二次注射ＩＴＣ,第３组同期发情率较高（Ｐ<０．０５）,产羔率以第２组为高（Ｐ<０．０５）,成活则以第４组和第６组８为最高（Ｐ<０．０５）。试验初步结论为：三合激素与ＰＮＩＳＧ、ＬＲＨ─Ａ＿２配合处理,可利用于绵羊同期发情。     

孕马血清加前列腺素提高乏情母猪受胎率的试验

应用孕马血清促性腺激素（ＰＭＳＧ）结合注射前列腺素（ＰＧ）对１６头不发情的后备母猪和体况中等、偏肥、偏瘦经产母猪进行诱导发情。乏情母猪总发情率为７５０％、总受胎率为８５７％。乏情后备母猪的发情率和受胎率均为１００％;经产乏情母猪的发情率为７１４％,受胎率为８００％;膘情偏瘦和偏肥乏情母猪的发情率和受胎率分别为６３６％和６６７％。表明了ＰＭＳＧ结合ＰＧ处理能够成功诱导乏情的后备母猪和各种体况的经产母猪发情,并提高其受胎率。     

维生素ADE和孕马血清促性腺激素分别结合前列腺素对母黄牛面？…

应用孕马血清促性腺激素（ＰＭＳＧ）和维生素ＡＤＥ（Ｖｉｔ．ＡＤＥ）分别结合前列腺素（ＰＧ）对１２９头母黄牛进行同期发性定时输精试验。试验分组比较了ＰＭＳＧ＋ＰＧ、Ｖｉｔ．ＡＤＥ＋ＰＧ和ＰＧ处理组母牛之间的同期发情率和受胎率。结果表明三个处理组中采用ＰＭＳＧ结合ＰＧ促使母黄牛同期发情效果最好,明显高于采用Ｖｉｔ．ＡＤＥ结合ＰＧ（Ｐ〈０．０１）和ＰＧ（Ｐ〈０．０５）处理组;相对于ＰＧ处理组,采用Ｖｉｔ     

不同超排方法对中国美利奴羊超排效果试验

用３２只中国美利奴成年母羊（５～６岁）分５组进行不同超排药物的超排试验，凭借腹腔内窥镜观察卵巢的反应，并借助其进行手术取卵。经观察：ＦＳＨ各组超排效果好于ＰＭＳＧ组，超排有效率也高于ＰＭＳＧ组（Ｐ＜０．０１）；使用ＦＳＨ各组的超排效果不尽相同，其中Ｃ组ＦＳＨ（７．５ｍｇ）＋ＬＨ，Ｄ组ＦＳＨ（１１．５ｍｇ）＋ＬＨ和Ａ组ＦＳＨ（７．５ｍｇ）＋ＬＲＨ－Ａ３组的可用胚比单用ＦＳＨ的Ｂ组高，未排卵泡数则显著低于Ｂ组（Ｐ＜０．０１）；在Ａ、Ｃ、Ｄ三个处理组中，Ｃ、Ｄ两组的可用胚数高于Ａ组（Ｐ＜０．０１）。试验结果表明，用ＦＳＨ＋ＬＨ处理的Ｃ、Ｄ两组超排效果较好，且组间无明显差异（Ｐ＞０．０５）。     

生殖激素制剂对母猪诱导发情效果研究

用ＰＸ－７００制剂对爱情母猪进行诱导发情处理，并与三合激素、Ｐ、Ｇ．６００进行了比较，结果表明，母猪受配率、受经均高于后两种催情激素；母猪产仔数、受胎率与自然发情母猪无显著差异（Ｐ〉０．０５），对母猪的正常繁殖性能无影响。诱导发情母猪９４．１２％集中在注射ＰＸ－７００制剂的４８～１２０小时内发情，其发 峰集中在注药后７２～１２０小时（８７．０６％），ＰＸ－７００制剂可促使母猪的发情同期化。同时，讨     

雌酮诱导间情期毕格犬发情受胎的内分泌特征

选用成年、健康毕格雌犬６只，雄犬３只，用雌酮、ＰＭＳＧ、ＨＣＧ诱导间情期犬发情、受胎。结果表明：６只雌犬中５只出现明显情征兆，发情率８３．３％；其中３只受胎，受胎率为６０％，平均产仔４．７只。毕格犬血浆睾丸酮水平间情期平均为０．８１±０．１６ｎｍｏｌ／Ｌ高于其他期。     

The appearance of ovarian cysts in young sows after treatment with gonadotropin preparations for estrus induction

In 72 (46%) of 155 gilts discarded for genetic reasons after performance testing and housed under fattening conditions no heat could have been detected during the first 30 days. The gilts were assigned alternatingly to a control group and four different treatments of delayed puberty. The induction of puberty was carried out by injections of 1000 iu PMSG, 400 iu HCG and 2 mg oestradiol benzoate, 400 iu PMSG and 200 iu HCG and 800 iu PMSG and 400 iu HCG. If there was no estrus gilts were slaughtered 12 days later for examination of the ovaries. Those coming into estrus were slaughtered 8 days after disappearance of estrus. Estrus could be induced in 69 to 94% of the gilts, whereas 40% of the untreated showed estrus signs. After treatment with PMSG and HCG in 40 and 87% of the gilts cysts were found whereas none of the untreated and 26 and 29% of those treated with PMSG und HCG + oestradiol benzoate revealed ovarian cysts. In addition, those gilts that had come into estrus during the first 30 days were given injections of either 1000 iu PMSG or 800 iu PMSG and 400 iu HCG. The injections were made either on the 5th, 10th or 15th day of cycle. In both latter groups significantly more gilts showed standing heat than after treatment at cycle day 5. The results of inspection of the ovaries at slaughter and steroid hormones could not be assigned to a defined stage of the physiological ovarian cycle.(ABSTRACT TRUNCATED AT 250 WORDS)     

Oestrus synchronisation and fertility in black Bengal goats following administration of progesterone/prostaglandin and gonadotrophins.

Oestrus synchronisation, fertility and kidding behaviour were studied in 44 Black Bengal goats. They were divided into six experimental groups: group 1, control; group 2, progesterone; group 3, progesterone, pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG); group 4, prostaglandin F2 alpha (PGF2 alpha); group 5, PGF2 alpha, PMSG and HCG; group 6, PMSG and HCG. There was 100 per cent synchronisation of oestrus in the groups treated with progesterone, progesterone with PMSG and HCG, and prostaglandin with PMSG and HCG. In the other two treated groups the synchronisation was between 66 and 75 per cent. In the control group only 50 per cent of the animals came into oestrus during the period of observation. The duration of oestrus varied between 19 and 24 hours except in group 5 where it was 40.87 hours. Animals came on heat between 95 and 137 hours after treatment except in group 5 where the interval was only 18.87 hours. A maximum fertility of 75 per cent was observed in group 4 while the kidding percentage was greatest in group 2. There appeared to be no beneficial effect of superovulation on the number of kids produced. Gestation length was similar in all the groups.     

LRH-3、PMSG、HCG对人工授精母蓝狐产仔效果影响的比较(Ⅲ)

通过PMSG +LRH - 3和PMSG +HCG的组合使用 ,结果表明 ,使用PMSG +LRH - 3的试验组母狐的产仔率比对照组提高了 2 0 % ,胎平均产仔数提高了 0 2只。而使用PMSG +HCG组合母狐的产仔率与对照组相同 ,但胎平均产仔数提高了 0 5只。     

Possibilities of ovulation synchronization in puberty-induced gilts

Puberty was induced in 39 clinically prepuberal gilts (two groups of three sub-groups each) by parallel but locally separated application of 500 IU PMSG ("Maretropin") and 250 IU HCG ("Gonadex"), with the view to testing ways to synchronise ovulation. Seventy-two hours were allowed to elapse, before 24 animals received another application of 500 IU HCG and 15 animals 250 IU HCG. The animals were slaughtered in consecutive groups of study ovulation and histolotically examined to disclose endometrial processes. Ovulations were found to be well synchronised in the recipients of a second injection of 500 IU HCG. Only sub-threshold effects with no synchronised ovulation were recorded from the animals that had received a second dose of 250 IU HCG. A second injection of 500 IU HCG should be given not until something between 78 and 82 hours after puberty induction for optimum follicle maturation and adequate proliferation of the endometrium.     

Histological findings on the uterus of gilts following synchronized ovulation

Histological studies were undertaken with the view to testing uterus structure and function of gilts following synchronised ovulation by means of different PMSG doses. All proband groups received 500 I.U. HCG. All histomorphological, histochemical, and histometric checks revealed 500 I.U. PMSG to be too low a dosage, whereas the optimum amount was found to be between 750 and 1,000 I.U. PMSG. The uterine glands of all treated animals in all three groups were less favourably developed than those of the untreated controls. The best morphologico-histochemical pattern was observed following administration of 750 I.U. PMSG.     

Endocrine pathogenesis of postweaning anestrus in swine: response of the persistently anestrous sow to hormonal stimuli

The endocrine function of the individual components of the hypothalamo-hypophyseal-ovarian axis of the postweaning anestrous sow was evaluated by monitoring the sow's response to exogenous estradiol, gonadotropin releasing hormone (GnRH), and gonadotropins. Sows (4 to 6/group) not returning to estrus by 42.8 +/- 3.1 days after weaning were assigned to 1 of the following treatments: 10 micrograms of estradiol benzoate (EB)/kg of body weight; 200 micrograms of GnRH, 1,000 IU of pregnant mare's serum gonadotropin (PMSG); 1,000 IU of human chorionic gonadotropin (HCG); or 4 ml of saline solution plus 2 ml of corn oil. A preovulatory-like surge of luteinizing hormone [(LH) greater than 12 hours in duration] was observed in all weaned sows treated with EB. All EB-treated sows exhibited estrus and ovulated but none conceived. Sows given GnRH had transiently increased (less than 3 hours) LH concentrations that were not associated with estrus or ovulation. Treatment with PMSG caused an increase in peripheral concentrations of 17 beta-estradiol that was followed by an LH surge, estrus, ovulation, and conception. Treatment with HCG caused an increase in circulating concentrations of 17 beta-estradiol that was accompanied by a surge of LH in some sows and ovulation in all sows. Not all sows treated with HCG exhibited estrous behavior, but conception occurred in 2 of 3 sows that were mated at estrus. None of the sows treated with saline plus corn oil had consistent changes in circulatory concentrations of 17 beta-estradiol or LH and none exhibited estrus or ovulated.(ABSTRACT TRUNCATED AT 250 WORDS)     

对C57BL／6小鼠超排效果的研究

本文以近交系C57BL／6小鼠为实验对象．研究注射不同剂量的PMSG和hCG对小鼠超排效果的影响。取C57BL／6小鼠各30只,按照注射剂量不同分为A、B、C三组,每组10只,A组注射PMSG2．5IU,HCG2．5IU,B组注射PMSG5．0IU,HCG5．0IU,C组注射PMSG7．5IU,HCG7．5IU。每只小鼠腹腔注射PMSG,间隔48h后分别注射HCG进行超数排卵,再与性成熟同系公鼠合笼,次日早上检查阴道栓．有栓雌鼠用颈椎脱臼法处死。在实体显微镜下由输卵管膨大部冲卵．收集卵母细胞置于盛有M2培养液的表面皿中检查计数．分析超排效果。结果表明。C57BU6小鼠B组的平均取卵数极显著高于A组的平均取卵数（P〈0．05）;B组的平均取卵数显著高于C组的平均取卵数（P〈0．05）;C组与A组的平均取卵数差异不显著（P〉0．05）。     

不同剂量生殖激素对小鼠超排效果的影响

为了探讨孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)对小鼠超数排卵的最佳组合剂量、最佳注射时间间隔,本试验以成熟实验小鼠为试验动物,分别采用不同剂量PMSG(5IU、7.5IU、10IU、12.5IU)与不同剂量HCG(5IU、7.5IU、10IU、12.5IU)组合注射小鼠进行排卵效果研究,并以44h、46h、48h、50h的间隔时间对小鼠进行超数排卵处理试验。结果表明,(10+10)IU剂量组合处理的见栓小鼠平均获卵数最高(10.3枚);在注射间隔为48h试验组中见栓小鼠平均获卵数最高(8.4枚)。提示本试验条件下,小鼠超排中PMSG与HCG组合剂量为10IU、注射时间间隔为48h时超排效果较好。     

Studies on the effect of the HCG injection date on the fertility of ovulation-synchronized gilts

The time gap between pregnant mare serum gonadotropine injection (PMSG) and subsequent human chorionic gonadotropine injection (HCG) had major effects, within 72 to 80 hours, on the number of animals with recorded toleration reflex to deadline-oriented insemination as well as on actual fertility. Delay of injections within the above limits always led to higher farrowing and piglet rates. HCG injection was round to be optimally timed when 78-80 hours were allowed to elapse from the preceding PMSG injection.     

LRH-3、PMSG、HCG对人工授精母蓝狐产仔效果影响的比较(Ⅱ)

对人工授精母狐重复使用LGH -3和HCG的产仔效果的观察 ,结果表明 ,重复注射LRH -3和HCG母狐的产仔效果要好于 1次注射 (研究Ⅰ ) ,其中以重复注射LRH -3的效果为最好。