The effect of density in larval rearing of the pullet carpet shell Venerupis corrugata (Gmelin, 1791) in a recirculating aquaculture system

The pullet carpet shell Venerupis corrugata is an economically valuable species in several European countries, however, nowadays stocks are under high fishing pressure. Hatchery production of juveniles for release is a major contributor to strengthen the stock and consequently improve the sustainability of the natural stocks. This study aimed to determine the feasibility of rearing V. corrugata larvae with different larval densities (10, 40 and 200 larvae per mL) in a recirculating aquaculture system (RAS), compared with the traditional larval rearing methodology (Batch). The mean survival, growth and metamorphic rate of V. corrugata larvae in RAS was higher (11.1%; 71.3 μm; 21.6% respectively) than in the Batch system, in all tested densities. The larval growth was not affected by the initial density until 40 larvae per mL, however, 200 larvae per mL decreased the larval growth in length nearly 54 μm. The larval rearing time was shortened in 2 days in the RAS system. The physical, chemical and microbiologic parameters suggested that the tested densities were not excessive to disturb the biofilter stability of RAS. The V. corrugata larval rearing performed at high larval stocking densities in RAS system present a reduction in the operating costs to produce this species.     

罗氏沼虾早繁苗中间培育及商品虾两茬养殖技术探讨

在上海地区进行罗氏沼虾商品虾两茬养殖，关键是抓好早繁苗的中间培育和第二茬养殖用苗的囤养。采用温室保温、锅炉增温和气泵充气等技术措施对罗氏沼虾的早繁苗进行集约化中间培育，可以提前１个月，在５月１０日左右为商品虾的第一茬养殖培育出大规格虾苗。利用室外土池囤养晚苗，为第二茬养殖培育虾苗，重点应处理好饲料投喂与水质调节之间的矛盾。     

不同饲养方式对肉鸡生产性能的影响

选择1日龄罗斯308肉鸡12000只随机分为3组,在相同营养条件和饲养管理程序下,分别进行笼养、地面平养、棚架饲养,研究了3种饲养方式对肉鸡生产性能的影响。试验结果表明:49日龄时肉鸡体重笼养最高,其次是地面平养,棚架饲养最低,分别为2.6kg、2.5kg、2.4kg;肉鸡的料肉比笼养最低,其次是棚架笼养,地面平养最高;腿病的发生率笼养最高,其次是棚架饲养,地面平养最低;肉鸡出栏率笼养最高,其次是棚架饲养,地面平养的肉鸡出栏率最低。综上所述,笼养和棚架饲养与地面平养相比,能提高肉鸡增重和出栏率,降低料肉比。     

Estimation of flow in a rearing tank of marine fish larvae by simplified numerical computation—a case of two-dimensional flow

Marine fish larvae are fragile against physical stress. However, few studies have been conducted to evaluate the flow field in a rearing tank, which is assumed to provide a high degree of physical stress to marine fish larvae. The flow field in a rearing tank (volume of 1 m³) is generated by aerators, which are commonly used to provide oxygen.

This paper is a report on the estimation of stationary flow in the rearing tank of marine fish larvae. The larvae are seven band grouper larvae of Epinephelus septemfasciatus, which have a very low survival rate immediately after the hatching of eggs. The experiments of rearing of seven band grouper larvae were carried out using rearing tanks with four aeration rates (1000, 200, 50 ml/min, and no aeration). The effects of aeration on the survival and floating death of seven band grouper larvae were examined. The experiments confirmed that the mass mortality of seven band grouper larvae depends on the flow rate in the rearing tank. Aeration at 200 ml/min resulted in the highest survival and growth rates of grouper larvae.

Larvae-rearing experiments provided evidence that the flow rates of the rearing tanks are very important design aspects of rearing tanks. The estimation of flow in a rearing tank for an aerating rate of 200 ml/min was carried out by numerical calculation. The computation was simplified by a two-dimensional flow based on experimental results. The calculated flow in the rearing tank was compared with the experimental one. The calculation of the stationary flow in the rearing tank showed good qualitative and quantitative agreement with the experimental results. The numerical estimation of the flow in a rearing tank of marine fish larvae was confirmed to be effective and satisfactory for the design of a tank that would provide optimum performance.     

Culture methods of live algal feeds for European aquaculture: optimising culture conditions for Ulvella lens

The Japanese development of mass culture techniques for Ulvella lens during the 1980s for aquaculture has stimulated the development of rearing techniques for abalone and sea urchins in producing countries. However, since the late 1980s, there has not been any in-depth evaluation of culture methods for U. lens, nor the development of a new robust method for aquaculture operators. The use of this known inducer for settlement of veliger larvae has not been employed on a commercial scale in Ireland or in European aquaculture systems. The new methodology described here has produced a threefold increase in the settlement of sea urchins (Paracentrotus lividus) and is recommended for adoption by the European aquaculture industry.     

甲鱼越冬期的控温养殖

对照１９９５年１０月《全国甲鱼越冬期的健康养殖研讨会》的典型材料，介绍了有关甲鱼安全越冬的营养生理、养殖设施、环境与水质条件、饲养管理和健康养殖等方面的一些情况，提出了作者自己的看法。     

中华仙影海葵亲体驯养的初步试验

报道了中华仙影海葵亲体驯养的方法和实验培养的初步结果,描述了海葵亲体驯养的条件和管理方法,对饵料投喂和产卵规律进行了探讨,提出了中华仙影海葵亲体驯养的技术要点。     

UV treatment in RAS influences the rearing water microbiota and reduces the survival of European lobster larvae (Homarus gammarus)

The rearing environment is important for a stable production of good quality lobster juveniles. By providing an environment excluding pathogens and dominated by mutualistic bacteria, the probability of developing healthy host-microbe relationships and produce healthy juveniles is increased. Disinfection of water and sudden increase in the supply of organic matter in culture tanks are processes that open for uncontrolled microbial regrowth in the rearing water. This increase the variability in the development of the microbiota between replicate rearing tanks and promotes selection for potentially harmful opportunistic bacteria. In two start feeding experiments with European lobster (Homarus gammarus) we compared the bacterial environment in three types of rearing systems: a recirculating aquaculture system (RAS) with UV treatment directly in front of the rearing raceways, a RAS without disinfection, and a conventional flow through system (FTS). The RAS with no disinfection was hypothesised to stabilise the microbiota of the rearing water, select against opportunistic bacteria, and reduce variability in production outcome between replicate tanks compared to the other systems. As predicted, the three different systems developed significantly different compositions of the microbiota in the rearing water and the larvae. On average, the survival of larvae in RAS without disinfection increased with 43 and 275 %, in the first experiment, and 64 and 18 % in the second experiment, compared to RAS with UV and FTS, respectively. Also, the RAS without disinfection showed less variability in the survival of larvae between replicate tanks and batches compared to the other treatments. The results are promising for controlling the microbiota of the rearing water to improve, increase and stabilise the production of marine larvae by competent use of water treatment and selection regimes. Based on the presented and previous work, RAS is recommended over FTS, and in RAS it is recommended to avoid point-disinfection of the recirculating water, to provide a stable and beneficial microbial environment in the cultivation of marine larvae.     

Cost comparisons of rearing larvae of freshwater shrimp,Macrobrachium acanthurus and M. rosenbergii,to juveniles

Costs were determined for rearing larval Macrobrachium rosenbergii (De Man) and M. acanthurus (Wiegmann). Rearing was conducted in two phases. First-phase rearing (first 10 days) was conducted in conical fiberglass tanks with recirculated, filtered, brackish water; second-phase rearing (final 30 days) was done in aerated 1 000-l concrete tanks. To minimize water quality problems and debris accumulation in second-phase rearing, larvae were transferred every 4 to 5 days to a new tank of clean water. Larval food consisted of newly hatched Artemia and either freshly ground fish or freeze-fried fish.Mean survival in five M. rosenbergii rearing trials was 43% in 40 days at a final density of 12 juveniles per liter. Mean survival of four M. acanthurus trials was 25% in 44 days at a final density of 3 juveniles per liter. Average total cost of labor, operation (electricity), food and water per thousand juveniles produced was $3.56 for M. rosenbergii and $13.42 for M. acanthurus.Extrapolation of results to larger (3 000-l) second-phase rearing tanks showed M. rosenbergii rearing cost could be reduced to $\text{\$1.87}\text{1 000}$.     

Techno-economic evaluation of Asian seabass (Lates calcarifer) nursery rearing in small net cages (hapas) under different coastal salinities

Nursery rearing is the critical interim phase in farming of Asian seabass fish (Lates calcarifer), which produce fish fingerlings as an input for grow-out farming. The present study evaluated the techno-economic performance of seabass nursery rearing in low and high saline coastal waters. The results indicated that seabass nursery rearing is technically efficient with a mean technical efficiency of 99.83% and 92.45%, respectively under low and high saline conditions. The mean survival of young fishes was 63.50% and 42.50% with a mean daily weight gain of 0.08 g and 0.15 g, respectively in low and high saline waters. While the benefit cost ratio (BCR) was 2.76 and 1.9, the internal rate of return (IRR) was calculated to be 300% and 130%, respectively, indicating the economic viability of nursery rearing under different salinity regimes. Furthermore, it was observed that nursery rearing in low saline waters was more efficient and highly remunerative. In the Indian socio-economic scenario, a mean monthly income per person spending 2 h per day was found to be 129 USD and 317 USD respectively in high and low saline nursery systems which is a considerable earning. The results explicitly stated that nursery rearing itself is an exclusively livelihood development activity for the coastal fisher families with an active participation of fisher women. Establishment of finfish hatcheries to ensure continued supply of seabass seeds is the key factor in facilitating wider adoption of nursery rearing as a sustainable farming activity.

     

Improvement of the survival in the seven-band grouper Epinephelus septemfasciatus larvae by optimizing aeration and water inlet in the mass-scale rearing tank

The water flow in larval rearing tanks has been indicated to cause mass mortality of the seven-band grouper Epinephelus septemfasciatus larvae. Therefore, a new aerating method was tested in an actual scale intensive rearing tank (8.0 m in diameter, 1.87 m of water depth, 100 m³ of volume), in which an aerator was positioned at the center of the rearing tank surrounding cylindrical drain (1.2 m in diameter) to generate the flow field, and seven larval rearing trials were performed. The survival rate with the former aeration methods were compared, in which several aerators were located in the rearing tank. The survival rate at 10 days after hatching with the new aeration method (61.5±5.1%, n=7) was approximately three times higher than the former methods (21.2±13.7%, n=6). The flow environment of rearing tanks was also examined by quantifying the flow field, and the relationship between the flow field in the rearing tank, behavior of larvae and survival discussed. It was confirmed that the vertical circulating flow was observed in rearing tanks, and determined effectively the survival and the behavior of grouper larvae in patchiness.     

Response changes with growth in the gustatory receptors of young yellowtails (Seriola quinqueradiata)

SUMMARY: Laboratory-hatched juvenile yellowtails (mean fork length, 9.3 cm) were reared for 2 months in two groups on different kinds of food, mysid and squid in one group and fish in the other, and the gustatory neural responses to seven chemicals (adenosine-5'-monophosphate, alanine, arginine, betaine, proline, tryptophan, and valine) were recorded in fish before and after the 2-month rearing period. Large variances in the responses to some chemicals were noticed in the juveniles before the rearing experiment. Mann–Whitney U -tests on the neural responses indicated significant changes in the magnitude of the responses to valine and some other chemicals relative to that for proline or tryptophan between the pre- and the two post-rearing groups. No significant differences in the response magnitude for the seven chemicals were observed for the two post-rearing groups suggesting that the response changes during the 2-month rearing might have been intrinsic and not due to specific food items in the diet. The dose–response relationship for some chemicals was also examined in the juveniles before and after the 2-month rearing. A slight lowering of threshold was noticed for alanine and valine after the rearing. Data on the responses of wild yellowtails were in support of the changing responsiveness of gustatory receptors during development of juvenile yellowtails.     

An evaluation of DVAqua®, a fully‐fermented yeast culture,during long‐term hatchery rearing of McConaughy strain rainbow trout

The addition of a proprietary, fully‐fermented yeast Saccahromyces cerevisiae culture supplement (DVAqua^®, Diamond V Mills, Cedar Rapids, IA, USA) was evaluated during long‐term feeding of McConaughy strain rainbow trout Oncorhynchus mykiss. Beginning at initial feeding and continuing for 408 days of hatchery rearing, the trout received either a commercially‐manufactured feed or the same feed containing 0.125 g kg^?1 DVAqua. This study was conducted at a production level as part of normal (real‐world) hatchery operations, with the fish periodically inventoried and moved into different rearing units. Although no rearing‐tank replication occurred during the first 54 days of feeding, multiple tanks and raceways were used thereafter. Fish in rearing units receiving DVAqua supplementation exhibited less mortality, particularly during the earlier rearing stages. During the final 177 days of rearing in six raceways, DVAqua‐fed McConaughy strain trout were significantly larger and had a significantly improved feed conversion ratio. The overall feed conversion ratio for the entire duration of the study was 1.17 in the fish receiving DVAqua supplementation compared to 1.21 in the control group. Despite the limitations of this study, the use of DVAqua is recommended for McConaughy strain rainbow trout and other less‐domesticated, more difficult‐to‐rear salmonids.     

Rearing performance of Clarias batrachus larvae: Effect of age at stocking on growth and survival during fingerling production

An experiment was conducted to evaluate the rearing performance of different larval stages for optimum growth and survival during fingerling production of Clarias batrachus with an aim to reduce the rearing time of the catfish larvae in a hatchery system. The larvae were reared for 5, 10 and 15 days before stocking them in the nursery tanks. No significant (P < 0.05) difference could be found in length increment at the end of first week of rearing. But the total length was reduced during rest three weeks in five days old larvae compared to that of other two higher age groups. In 5 days old larvae the reduced weight (P < 0.05) was observed during the entire rearing period. However, the specific growth rate (SGR) did not vary among the groups. The survival rate as well as total biomass was decreased while stocking five days old larvae for rearing, compared to that of other two age groups.     

奥尼罗非鱼养殖池沉积形成及其主要影响因子的初步研究

在半封闭式奥尼罗非鱼养殖池中投入饲料,探索养殖过程中鱼池沉积形成及其主要影响因子。试验结果表明:无论养鱼或未养鱼,投饲或不投饲,各池均发生不同程度的沉积;外源饲料输入量相同时,养鱼池新颗粒物生成量(干重)比未养鱼池大15.63%,而沉积量比未养鱼池小19.49%;无外源饲料输入时,养鱼池的沉积量比未养鱼池大75.76%;养殖系统内养殖对象与其所处环境的相互依存和相互制约对系统新颗粒物生成及沉积具有重要作用。     

Improvement of techniques for carp fry production in the laboratory

The paper describes field experiments in Madagascar on the artificial propagation of common carp and on rearing larvae. It concerns the preparation of brood fish for spawning, induced spawning, artificial propagation and incubation techniques, and intensive larval rearing.     

海带工厂化低温育苗法及其科学技术基础

海带工厂化低温育苗是一项综合性技术措施,其中心环节是海带的“夏繁”和配子体、幼孢子体的“越夏”。围绕着这二个中心环节,试验研究取得了夏熟种海带培育、集约式平面采光、人工附着基的改进和制作、育苗期间的温光控制和制冷工艺、病害防治、以及水的循环与净化等配套技术和成果,有效地促进了海带养殖业的发展。本试验根据海带生物学特性,以人工模拟的环境条件,结合考虑经济上的合理性,培育出大量幼苗,这对海洋及内陆水域中其他生物资源的开发利用提供了有参考价值的途径。     

Variations in Bacterial Community of Rearing Water and Gut of Common Dentex,Dentex dentex (Linnaeus 1758), Larvae Using Three Microalgae Management Approaches

Microorganisms present in the rearing water colonize the gut of first feeding larvae and represent the first barrier against opportunistic pathogens. The aim of the experiments presented herein was to standardize a protocol for the management of rearing water and microalgae suitable for the larval rearing of common dentex. In Experiment 1, bacteria–algae interactions were tested using a monospecific microalgal community, Tetraselmis chuii, suitable for nutritional experiments and with known antibacterial activity. In Experiment 2, the evolution of the bacterial community and larval performance (growth and survival) were monitored daily, in three conditions: (1) mature water: T. chuii was added 5 d before the rearing of common dentex larvae, (2) green water: T. chuii was added 1 d before, and (3) clear water: no T. chuii addition. The results show the influence of the presence of T. chuii on the evolution of the bacterial communities, both in terms of bacterial density and morphology, and indicate green water is the most suitable water treatment for management of larval rearing for common dentex.     

Controlled hatchery production of African catfish, Clarias gariepinus (Burchell): an overview

Abstract In order to promote the successful supply and satisfactory survival of African catfish, Clarias gariepinus (Burchell), it is advocated that the fish be reared in hatcheries until they begin air breathing. Research conducted over a number of years to develop culture technology for intensive hatchery rearing to the stage of air breathing is brought together here as an overview. In particular, the definition of early development stages is considered as well as induced spawning, egg incubation, larval rearing and fry rearing.     

Biological Performance of Red Porgy (Pagrus pagrus) Larvae under Intensive Rearing Conditions with the use of an Automated Feeding System

Pagrus pagrus is one of the promising species for the industry in the Mediterranean but its rearing is still far from satisfactory. In the present work, the conditions and results of larval rearing with the use of an automatic feeding system are presented. Eight populations were reared for 20 days with the pseudo green water methodology in two successive trials. Larvae were fed enriched rotifers during the entire rearing period, a mixed diet of rotifers and Artemia (Instar I when larvae reached 5 mm in length, replaced by Instar II after larvae reached 5.5 mm) and a formulated diet (after day 16 post hatching). A computerized system for feeding management was used. A total of 388,000 eggs were incubated and after 20 days, 237,973 larvae (TL = 6.96 ± 0.17 mm) were produced with a survival rate of 61.4 ± 6.3%. Individuals grew in terms of wet weight with an exponential rate of 0.167 ± 0.008 daily (R ²= 0.983) with no variations between replicates. Mean individual daily consumption at first feeding (day 4-post hatching), was 0.20 ± 0.06 mg of food (0.03 ± 0.01 dry weight), that is, 180–300 rotifers, while on day 20, consumption increased by 10 (2.081 ± 0.106 mg and 0.276 ± 0.014 in wet and dry weight, respectively). When compared with sea bream, consumption was higher by approximately 3–4-fold. The mean food conversion ratio of the experimental period was 4. In comparison with sea bream larviculture, food consumption of red porgy is higher, and a different feeding strategy is required to satisfy the requirements of the larvae.