低磷红壤无机磷组分特征及间作调控作用

为明确间作和施用磷肥对低磷红壤无机磷组分的影响,促进酸性土壤磷肥高效利用,通过田间小区定位试验,采用玉米单作（MM）、玉米//大豆间作（MI）2种种植模式,在4个施磷水平（P2O5 0、60、90、120kg·hm-2,分别记作P0、P60、P90、P120）下,研究玉米//大豆间作根际土壤无机磷组分变化特征,探讨间作对红壤玉米产量及其可持续性、磷吸收和磷素收支平衡、土壤无机磷组分的调控作用。结果表明,玉米籽粒产量及其可持续性指数（SYI）、磷吸收量随着施磷水平的增加而增加,在P90水平达到最大值。低磷红壤无机磷组分以O-P和Fe-P为主,二者占无机磷库的71.44%～80.18%,施用磷肥显著提高了红壤Fe-P和Al-P的含量与占比。玉米//大豆间作显著提高玉米产量,促进磷的吸收利用,减少土壤磷素盈余量,显著降低红壤O-P、Fe-P、Al-P含量。与单作相比,在P60、P90、P120水平下,间作玉米产量分别显著提高66.70%、52.44%、74.14%,SYI分别显著提高55.31%、42.18%、78.00%,磷吸收量分别显著提高61.97%、48.67%、74.33%,土壤磷...     

解磷菌剂对作物生长和土壤磷素的影响

利用课题组自行分离筛选的B2和B67解磷菌株研制的菌剂，在盆栽和大田试验过程中，菌剂在增加作物产量的同时，有提高土壤速效磷含量、培肥土壤的作用，而且是菌剂和化肥配合施用效果显著。施用了解磷菌剂或菌剂和化肥配合施用后，盆栽玉米的株高、鲜重和干重，较CK增加15．2％～89．2％，玉米单株吸养量较CK增加20％～146％；青菜的鲜重、干重和生物量较CK增加15．8％～41．6％，和CK比较差异显著；小麦的株高、有效小穗数、穗粒数、粒重以及生物量也较CK差异显著，大田试验的几种作物也有不同程度的增产。另外B2和B67接种到山西省典型土壤中，土壤速效磷含量较CK增加1．35～3．04倍，且发现其溶磷效果和土壤磷酸酶活性与有效活菌数相关，解磷菌剂在提高土壤速效磷含量的同时，也有提高土壤速效钾含量的作用。     

长期不同供磷水平驯化的土壤微生物的遗留效应--基于对植物生长、养分吸收、土壤酶活性及菌根特征的分析

在集约化农田生态系统中,长期过量施用磷肥导致土壤中磷的累积,是否存在累积磷驯化（training）微生物的遗留效应。本研究依托长期定位玉米试验地（始于2007）,以长期不同供磷水平（来源于施用P2O5 0、75、300 kg·hm-2的处理,分别为P0、P75、P300）石灰性土壤中的微生物为研究对象,通过盆栽接种微生物,探究不同磷供应驯化下的土壤微生物,在两个基质供磷水平（不施磷No P 和+P 30 mg·kg-1）下对玉米和三叶草植物生长、养分吸收、土壤酶活性及菌根特征的影响。结果表明,菌剂类型（灭菌处理,原位菌剂）显著影响玉米和三叶草地上部生物量和磷吸收量。在两个基质磷水平处理,相比于灭菌菌剂处理,接种原位菌剂显著提高了三叶草地上部生物量和磷吸收量,但三种菌剂之间差异不显著。 在No P条件下,相比于灭菌处理,接种原位菌剂对玉米地上部生物量无显著影响。在+P条件下,接种原位菌剂显著降低了玉米的生物量;在两个基质供磷水平下,玉米地上部生物量在P300处理中显著高于P0和P75。菌剂和基质供磷水平共同影响土壤酶活性。与P0和P75相比,P300菌剂显著降低了三叶草土壤中过氧化物酶活性,提高了No P条件下玉米土壤中过氧化物酶和几丁质酶的活性。在No P条件下,接种P75显著提高了玉米土壤中酸性和碱性磷酸酶的活性。两种作物的菌根特征不同,接种位P300三叶草根系丛枝菌根真菌的侵染率显著低于P0和P75,而玉米根系的侵染率在三种菌剂之间差异不显著。综上,基质供磷水平以及植物种类共同影响累积磷驯化土壤微生物的效应,说明磷肥管理需要考虑植物-微生物的特性。     

滴灌条件下不同磷肥品种对土壤磷有效性及玉米产量的影响

通过模拟试验及田间试验研究不同磷肥施用后对土壤磷有效性的动态变化和分布特点,以及玉米磷吸收和产量响应的影响。田间和模拟试验包括4个处理,分别为对照(不施磷肥)、MAP(磷酸一铵)、UP(磷酸脲)和AAP(水溶性聚磷酸铵)。模拟试验中,不同磷肥均提高土壤中有效磷含量,APP处理10—15 cm土层有效磷含量增加最多。在均匀的养分投入和管理水平下,3种磷肥品种均有土壤酸化效果,其中UP处理最为显著。田间试验中,玉米生长后期,APP处理的土壤有效磷含量在深层土壤分布较高,UP处理土壤pH较低,均有利于改善土壤的供磷能力,提高土壤有效磷含量;APP处理对根系发育有显著的促进作用,玉米组织磷含量和磷吸收量最大,产量最高(14.40 t/hm^2)。3种磷肥中,APP处理移动性较好,土壤深层有效磷含量增加,利于根系生长和养分的吸收,提高产量。水溶性聚磷酸铵(APP)是一种适合于玉米灌溉施肥的肥料。     

农业废弃物制备的生物质炭对红壤酸度和油菜产量的影响

利用自行研制的生物质炭化炉在田间条件下制备花生秸秆炭和油菜秸秆炭,采集秸秆气化站产生的稻壳炭,研究了这3种生物质炭对酸性土壤的改良效果和对油菜产量的影响。结果表明:施用稻壳炭、花生秸秆炭和油菜秸秆炭均可提高土壤p H,降低土壤交换性酸含量,效果随施用量的增加而增强。生物质炭对酸性土壤的改良效果主要决定于其本身的含碱量,施用花生秸秆炭和油菜秸秆炭显著增加土壤交换性盐基阳离子、有效磷、有效阳离子交换量和盐基饱和度,并提高油菜籽产量。田间条件下施用花生秸秆炭和油菜秸秆炭3年后土壤p H仍明显高于对照处理,说明生物质炭对土壤酸度的改良具有持续性。因此,花生秸秆炭和油菜秸秆炭是优良的酸性土壤改良剂。     

生物炭对砂质潮土养分及玉米产量的影响

为了探索生物炭对砂质潮土中低产田的改良效果,采用田间小区试验,设三个生物炭梯度8 thm-2、16 thm-2和32 t hm-2,研究了生物炭施用对砂质潮土养分,微生物数量及玉米产量的影响。结果表明:施生物炭后土壤含水量显著高于空白对照(P0.05),其中以32 thm-2施用量含水量最高,最高比不施生物炭对照(CK)高5.4%。施用生物炭显著增加了土壤pH值,施用量越大pH增加值越高,开花期后pH开始缓慢下降;施用生物炭土壤铵态氮含量总体变化不明显,硝态氮含量显著低于CK,施用量越高,硝态氮含量越低;施生物炭明显提高了土壤中速效钾含量(P0.05),而对速效磷影响较小,后期有降低土壤速效磷的趋势;各梯度施用生物炭对试验土壤细菌数量没有显著影响(P0.05),较大施用量16 t hm-2和32 t hm-2显著提高了真菌数量(P0.05)。施用生物炭16 thm-2和32 thm-2可显著提高玉米地上部干物质积累量和产量,二者之间差异不显著(P0.05)。     

长期定位施肥对亚热带丘陵地区红壤旱地质量的影响Ⅰ.酸度

研究了中国科学院红壤生态实验站长期定位施肥试验5种不同施肥处理对土壤pH、土壤交换性氢、铝的影响,及其与土壤有机质之间的相关性。结果表明,长期不同施肥处理均提高了土壤pH,降低了土壤交换性氢和交换性铝含量,改良了红壤的酸性。施用有机肥(M)、有机肥+菌剂(BM)、有机肥+菌剂+微量元素(BMT)显著优于施用化肥(F)和化肥+微量元素(TF)的处理。添加微量元素和有效菌剂后土壤交换性氢、铝含量略有提高。土壤有机质与土壤pH呈显著正相关,与土壤交换性氢含量的相关性不显著,而与土壤交换性铝含量呈显著负相关。随着土壤有机质含量的增加,土壤交换性氢占土壤交换性酸度比例越大。长期施用有机肥、提高土壤有机质含量是改良红壤酸度和减轻铝毒较好的农艺措施。     

溶磷菌剂对玉米幼苗生长及根际土壤细菌群落结构 和磷素形态的影响

以FD11(普瑞斯特氏菌Priestia sp.)和CH07(阿氏芽孢杆菌Bacillus aryabhattai)两种溶磷菌剂为试验材料,进行菌剂灌根盆栽试验,测定溶磷菌剂施用对玉米生长、根际土壤养分、土壤微生物特性及土壤不同形态磷含量的影响。结果表明,施用溶磷菌剂可改善根际土壤养分特征和微生物学特性,增加根际土壤的活性磷源含量,对玉米幼苗表现出良好的促生作用。与不施菌剂相比,FD11处理玉米幼苗的株高、茎粗、地上部干重分别增加31.88%、36.39%、104.00%;根际土壤碱解氮、有效磷、速效钾分别增加了21.90%、107.67%、5.77%。施用溶磷菌剂的土壤H₂O-Pi(Pi为无机磷)、NaHCO₃-P和NaOH-P含量增加,但HCl-Pi和Residual-P含量显著降低。施用溶磷菌剂改变了玉米根际土壤细菌的群落结构,在细菌属分类学水平上,不动杆菌属、假单胞菌属、海洋杆菌属、鞘氨醇单胞菌属、节杆菌属等功能细菌的相对丰度显著增加。综合以上分析,施用溶磷菌剂显著提高了土壤速效养分含量,改变了根际土壤细菌的群落结构,增加了活性磷源的...     

不同自生固氮菌对盐碱地玉米生长与土壤养分特性的影响

为探讨4种自生固氮菌,包括菌c:争论贪噬菌(Variovorax paradoxus),菌d:玉米固氮螺菌(Azospirillum zeae),菌f:苜蓿中华根瘤菌(Sinorhizobium meliloti)和菌j2:荧光假单胞菌(Pseudomonas fluorescens)对盐碱地玉米生长和土壤养分特性的影响,在田间微区试验条件下,研究了土施4种固氮菌对玉米大口期和成熟期不同器官氮素积累量及成熟期产量与产量构成因素的影响,并探讨了4种菌对土壤有效氮含量和土壤脲酶活性的影响。结果表明:菌剂处理可以增加大口期与成熟期玉米根、茎与叶的干物质质量,提高大口期玉米根冠比;增加玉米籽粒中氮素积累量,其中f与j2处理较CK差异显著;各菌剂处理玉米产量较CK分别增加了18.09%、77.91%、157.0%和108.1%,菌f和j2差异显著。不同菌剂处理均提高了土壤有效氮、速效磷和速效钾含量,在成熟期,d处理较CK土壤有效氮含量显著增加了24.32%。在大口期与成熟期,各菌剂处理均提高了土壤脲酶活性。综上,各菌剂处理能提高土壤有效氮含量,提高玉米产量。本研究可为研制滨海盐渍土专用微生物菌肥提供理论依据及技术支撑。     

施用白云石对酸性黄红壤作物产量及化学性质的影响

白云石改良酸性黄红壤三年定位试验结果表明:酸性黄红壤施用白云石可以降低土壤交换性铝的含量,提高土壤pH值和交换性钙、镁的含量,显著提高作物产量,且有明显的后效。第1—6季作物分别增产10.8%~13.4%、21.5%~48.6%、9.4%~16.2%、10.9%~44.6%、7.9%~22.0%和6.6%~29.8%。白云石施用量为600kg hm-2时,降酸作用可维持3季作物以上;白云石施用量为2500 kg hm-2时,降酸作用可维持6季作物以上。     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Properties of anaerobically digested and composted municipal solid waste assessed by linking soil mesofauna dynamics and nitrogen modelling

We have studied the effect of anaerobically digested (ADMSW) and composted municipal solid waste (CMSW) on mineralization and foodweb dynamics to verify the hypothesis that ADMSW would immobilize N right after addition to soil in contrast to addition with CMSW. Another hypothesis was that the mesofauna (enchytraeids and microarthropods) would stimulate N release from the decomposer community. We measured excretion of -N and urea-N from the mesofauna and hypothesized that enchytraeids would release urea. ADMSW and CMSW were amended to pots with sandy loam and barley. The pots were divided into treatments with or without mesofauna. Mesofauna, plant N and biomass, soil N and ergosterol (fungal biomass) were measured over a 113-day period of four equidistant samplings. Soil respiration, N mineralization and N release by the mesofauna were modelled from concurrent studies. ADMSW- and CMSW-treated soils initially (<20 days) immobilized N. The amendments did not increase plant growth substantially, and this was probably due to N-limitation in the early stages of plant growth. Enchytraeid abundance was about three times higher in ADMSW- than CMSW-treated soils, indicating that ADMSW contained more labile compounds, bacteria, and microfauna. The mesofauna did not affect N-content, but the cumulated -N excreted by the mesofauna was estimated to be substantial and about one-fifth of total plant N in ADMSW. An explanation for this discrepancy might be that in the absence of the mesofauna, other members of the detrivore and microbivore community performed the mesofauna’s function. Neither enchytraeids nor microarthropods excreted urea.     

Faculty positions: Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Faculty positions: Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Assessing the effectiveness of conservation measures: Resolving the “wicked” problem of the Steller sea lion

“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options.     

Soil-profile distribution of primary and secondary plant-available nutrients under conventional and no tillage

Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate.     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.