2016年江苏省猪口蹄疫监测结果分析

为了解江苏省猪口蹄疫感染和免疫情况,采用荧光RT-PCR和ELISA方法,对收集的8000份猪组织样品和2718份猪血清样品分别进行口蹄疫病毒核酸和O型口蹄疫抗体检测.数据显示,口蹄疫病毒核酸阳性率为0;O型口蹄疫抗体合格率为68.95%.结果表明,江苏省规模场和屠宰场猪口蹄疫防控情况较好;O型口蹄疫抗体合格率略低于70%,说明仍有个别场户的口蹄疫免疫工作存在瑕疵.     

临西县猪瘟病毒、口蹄疫病毒O型和A型抗体血清学检测与分析

为了解河北省临西县部分猪场猪瘟病毒和口蹄疫病毒疫苗免疫效果，本研究于2022年从临西县部分猪场采集血清样品550份，采用ELISA法分别检测猪瘟、口蹄疫病毒O型和A型抗体合格率，并进一步统计和分析数据。结果显示，该地区猪群口蹄疫O型和A型抗体合格率均超过90%，而猪瘟抗体合格率为77.3%，均达到国家70%以上合格率的要求。进一步分析发现，所有猪场/发育阶段猪群口蹄疫O型和A型抗体合格率差异较小，所有猪场合格率均高于80%；但不同猪场和发育阶段猪群猪瘟抗体合格率存在差异，部分猪场猪瘟抗体阳性率低于70%；保育猪和育肥猪较母猪群抗体阳性率低，分别为70.39%和66.67%。以上研究结果表明，临西县口蹄疫O型、A型和猪瘟疫苗免疫效果均较好，超过国家制定的最低标准，但仍然有个别猪场或猪群存在猪瘟疫苗抗体合格率不高的现象，应引起重视。     

2015—2018年安徽省定点猪场主要疫病病原和血清抗体监测

为全面掌握安徽省口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征和猪伪狂犬病等主要猪群疫病的感染及免疫状况,2015—2018年,从安徽省东部、中部和南部选取12个生猪养殖场,应用荧光定量RT-PCR和ELISA检测方法,连续4年对上述疫病病原和血清抗体进行检测。结果显示：每年均检测出口蹄疫3ABC阳性抗体,O型和A型口蹄疫免疫抗体合格率呈逐年下降趋势。猪瘟和高致病性猪繁殖与呼吸综合征免疫情况较好,抗体合格率均超过农业农村部规定的70%标准,高致病性猪繁殖与呼吸综合征病毒核酸阳性率呈逐年下降趋势。猪伪狂犬病在猪场中存在一定的流行,gE（野毒）抗体阳性率在0.74%~19.90%之间;gB免疫抗体合格率在35.64%~71.16%之间,合格率较低且呈逐年下降趋势。小型猪场的主要疫病免疫抗体合格率普遍低于中型猪场,保育猪群和育肥猪群抗体合格率低于其他生产阶段猪群。结果提示,该地定点猪场的口蹄疫和猪伪狂犬病防控效果不理想,不仅存在一定的病毒感染,免疫抗体合格率也偏低,病毒流行风险较高,需要加强监测和免疫;猪瘟、高致病性猪繁殖与呼吸综合征总体防控效果较好,但需要加强种公猪免疫。     

贵州省开阳县某猪场主要猪病的血清学抗体检测分析

为了解贵州省开阳县某猪场猪繁殖与呼吸综合征病毒、猪瘟病毒、猪O型口蹄疫病毒、猪伪狂犬病病毒的免疫及感染情况,通过采用ELISA检测方法对猪场30份血清进行相关抗体检测。结果:(1)猪O型口蹄疫病毒、猪伪狂犬病毒gB、猪瘟病毒免疫抗体合格率均>70%,免疫效果较好。(2)猪口蹄疫3-ABC抗体阳性率为0,表明猪群无口蹄疫病毒感染。(3)猪繁殖与呼吸综合征病毒免疫抗体合格率为26.7%,未达到国家农业农村部规定≥70%的要求,需加强免疫。(4)猪伪狂犬病病毒gE抗体阳性率为70%,表明猪群存在伪狂犬病病毒感染。结论:猪场需加强猪繁殖与呼吸综合征的免疫和防控,同时要做好猪伪狂犬病的检疫与净化工作。     

四川省部分规模猪场口蹄疫免疫效果的监测分析

为了解四川省部分规模猪场口蹄疫免疫效果,笔者从四川省内川东、川南、川西、川北、川中5个片区的17个规模猪场采集血清样品1 454份,采用液相阻断ELISA方法进行口蹄疫O型和A型免疫抗体检测。结果显示:FMD-O型抗体总阳性率为83.29%,FMD-A型抗体总阳性率为80.26%;17个规模猪场的FMD-O型、A型抗体场群免疫合格率均为76.47%;哺乳仔猪、保育猪、育肥猪、经产母猪、种公猪、后备猪的FMD-O型抗体阳性率分别为84.95%、68.67%、81.31%、94.23%、88.03%、100%,FMD-A型抗体阳性率分别为80.65%、60.96%、79.75%、93.53%、88.03%、100%。本试验表明,四川省规模化猪场口蹄疫免疫效果总体较好,但部分猪场的免疫抗体水平不高,因此规模猪场仍需加强监测和免疫,降低口蹄疫的发生风险。     

贵阳地区规模化猪场主要疫病免疫效果评估

为了解贵州省贵阳地区规模化猪场主要动物疫病的疫苗免疫情况,从贵阳市6个规模化猪场采集血清样本共576份,采用ELISA(酶联免疫吸附试验)方法,分别进行血清抗体检测。结果显示,6个规模化猪场猪瘟病毒、猪蓝耳病病毒、猪伪狂犬病病毒gE蛋白、猪伪狂犬病病毒gB蛋白、猪O型口蹄疫病毒和猪A型口蹄疫病毒抗体阳性率分别为94.62%、86.46%、8.06%、98.32%、91.85%和97.14%,抗体离散度分别为74.56%、62.35%、15.23%、50.54%、35.42%和43.68%。结果表明,贵阳地区6个规模化猪场的猪瘟病毒、猪蓝耳病病毒、猪伪狂犬病病毒gB蛋白、猪O型口蹄疫病毒和猪A型口蹄疫病毒的抗体阳性率都比较高,但是个别猪场也存在免疫抗体不合格。猪O型口蹄疫病毒和猪A型口蹄疫病毒的抗体离散度相对较低,免疫效果较好。其中猪场B、猪场C、猪场F出现了猪伪狂犬病病毒gE蛋白抗体阳性,提示猪群可能存在猪伪狂犬病野毒感染,猪场需要采用合适的免疫程序和适时开展疫病净化。     

安徽省2015年规模猪场高致病性蓝耳病定点监测与分析

[目的]掌握安徽省规模猪场高致病性蓝耳病的感染状况和免疫抗体水平。[方法]采用抗体阻断ELISA、荧光RT-PCR方法检测了24个猪场的588份血清样本和28个场点的790份组织样品。[结果]高致病性蓝耳病个体抗体免疫合格率为78.1%,群体免疫合格率为75.0%;中型猪场的平均免疫抗体合格率为87.1%、小型猪场为63.9%。高致病性蓝耳病病毒核酸个体阳性率为28.7%,群体阳性率为75.0%;中型猪场、小型猪场和生猪屠宰场的高致病性蓝耳病病毒核酸个体阳性率分别为41.7%、19.5%和6%。[结论]2015年安徽省规模猪场的高致病性蓝耳病抗体免疫合格率较高,但存在带毒现象,建议加强疫病的免疫和综合防控。     

台州市猪口蹄疫O型合成肽疫苗免疫情况调查与研究

对台州市的3个区24个猪场使用猪口蹄疫O型合成肽疫苗免疫情况进行了调查。临床使用情况表明,与以前使用的猪O型口蹄疫浓缩苗相比,使用该苗免疫对生猪的应激小、基本无免疫副反应;疫苗质量较稳定,抗体检测结果表明,首次免疫后15天效价较低,首免后30天检测阳性率平均为75.68%,高于部颁标准70%的抗体合格率;二免后15天、30天免疫抗体阳性率分别平均为90.81%和94.59%,因此经二次免疫后抗体效价才稳定上升,免疫效果确实。     

乐山市猪瘟和猪O型口蹄疫免疫抗体水平检测与分析

为掌握乐山市生猪饲养场猪瘟和猪O型口蹄疫疫苗免疫效果,2015年,笔者对全市11个区(市)县114个生猪养殖场(户)采用ELISA方法,检测猪瘟免疫抗体1917份、猪O型口蹄疫免疫抗体1881份。结果表明,乐山市猪瘟免疫抗体合格率为84.9%,猪O型口蹄疫免疫抗体合格率为81.1%。规模化猪场猪瘟和猪O型口蹄疫抗体合格率普遍高于散养户,试验为乐山防控这2种疫病提供了基础资料。     

2020年新疆规模化猪场猪O型口蹄疫病毒抗体检测与分析

为了解2020年新疆规模化猪场O型口蹄疫病毒抗体水平,本研究采用液相阻断酶联免疫吸附试验（LPB-ELISA）方法对采集的469份血清进行抗体检测。结果显示,猪O型口蹄疫病毒抗体平均阳性率为88.06%（413/469）。不同类别猪群抗体的阳性率均达到85.00%以上。结果表明,8个规模化猪场O型口蹄疫病毒抗体阳性率均达到国家标准（70%）,但1个规模化猪场阳性率仅为56.70%,需要制定更加合理的免疫程序。     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1991

In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium.     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

     

Heartworm in dogs in Canada in 1987

In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).     

Heartworm in dogs in Canada in 1986

In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.