细菌发酵大豆异黄酮甙元的工艺及高效液相色谱检测

大豆异黄酮是一类存在于大豆中能对人体发挥有益作用的非营养物质。β-葡萄糖苷酶是一种能将大豆中的异黄酮由结合型糖苷向具有生理活性的游离型苷元转化的活性酶[1]。据报道,发酵大豆制品中游离型糖苷的含量远高于未发酵的大豆[2],这说明在大豆加工发酵过程中某些微生物所产β-葡萄糖苷酶使糖苷向甙元进行了转化。本文用本课题组筛选的产β-葡萄糖苷酶的菌种,对大豆黄素(大豆异黄酮甙元的一种)发酵条件进行优化,并通过HPLC(高效液相色谱)进行检测。1材料与方法1.1材料大豆(购于武汉市中百仓储超市)、细菌B.SP-4(本课题组筛选)。1.2主要…     

Superoxide production by stimulated equine polymorphonuclear leukocytes-inhibition by anti-inflammatory drugs

Polymorphonuclear leukocytes (PMNLs) were isolated from an inflammatory exudate induced in the intercarpal joints of horses by an administration of carrageenin. Their superoxide production at rest and following stimulation with either serum-treated zymosan (STZ) or phorbol myristate acetate (PMA) was measured by cytochrome-c reduction. Stimulation of the cells increased the cytochrome-c reduction 10-15 times that of resting cells. The maxima were 20 nmol of reduced cytochrome-c per 10(6) cells per ml at 120 min (STZ) and 35 nmol of reduced cytochrome-c per 10(6) cells per ml at 60 min (PMA). The maximum inhibition of the cytochrome-c reduction by superoxide dismutase (Palosein) was 83.6% (STZ stimulation) and 72.1% (PMA stimulation). The non-steroidal anti-inflammatory drugs, phenylbutazone, salicylic acid, aspirin, sodium salicylate in addition to D-penicillamine and dimethylsulfoxide caused dose-dependent inhibition of the cytochrome-c reduction when the cells were stimulated by PMA. The maximum inhibitions were 64% and 36% for aspirin (10(-2) M), 32% and 17% for phenylbutazone (10(-3) M), 15% and 31% for dimethylsulfoxide (6.4 x 10(-1) M), 32% and 19% for salicylic acid (10(-2) M), 0% and 17% for sodium salicylate (10(-2) M) and 2.2% and 2.5% for D-penicillamine (10(-4) M) when the cells were stimulated by STZ and PMA, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)     

Proteolytic cleavage of immunoglobulin by enzymes released by Fasciola hepatica

Immature Fasciola hepatica release a papain or cathepsin B-like proteolytic enzyme which cleaves immunoglobulins (Ig) of mouse, rat, rabbit and sheep in vitro. Mouse IgG and IgM molecules are both susceptible to cleavage as is hemoglobin. Whether single or multiple proteases are responsible for Ig cleavage is unknown. The proteolytic activity of secreted enzyme(s) is optimal at pH 3.5-4.5, but activity is also present at pH 7. Proteolysis is enhanced in the presence of 5 mM dithiothreitol or 100 mM cysteine. Based on studies with protease inhibitors, the F. hepatica enzyme activity has been identified as a thiol protease. It is destroyed by heating at 56 degrees C for 1 h, but retains activity after storage at -20 degrees C for 7 days. Whether inhibition of the proteolytic activity increases the susceptibility of F. hepatica immature worm to any extant immune effector mechanisms in hosts remains to be determined.     

Colostrum production by ewes and the amounts ingested by lambs

Studies involving 49 ewes which were well nourished during the last eight weeks of pregnancy indicated that ewe weight loss during pregnancy and lamb birth weights followed the recognised pattern and were within acceptable "normal" limits. Colostrum production ranged from 1238 to 4593 g per ewe during the 48 h following the first suckling and there was evidence that production levels were related to demand by the lambs. Colostrum consumption by individual lambs was related to litter size. The smaller the litter size the greater was the amount ingested during the first 48 h of life. No clear relationship was established between the amount of colostrum consumed and the level of immunoglobulin in a lamb's circulation. Possible reasons for this are discussed.     

奶牛过瘤胃氨基酸的补加

最近的研究表明，补加氨基酸可减少奶牛日粮中过瘤胃蛋白质（ＵＤＰ）的供应量，同时提高总产奶量和乳蛋白含量。 在泌乳前期，奶牛的干物质采食量较低，而泌乳量较高。这就需要在日粮中有足够的可利用能量和蛋白质。现在瘤胃保护脂肪或过瘤胃脂肪被用来补充以谷类为基础的传统精料，为高产奶牛提供了可通过瘤胃的足够能量。相类似地提供足够的瘤胃微生物蛋白质，以便提供足够的氨基酸，从而使高产奶牛获得最大产奶量和乳蛋白。２０世纪９０年代早期，人们就发现蛋氨酸和赖氨酸是合成奶蛋白的限制性氨基酸，在此基础上奶牛营养学家借助计算…     

Death by cyathostominosis

In autumn 2002 and 2003, the Animal Health Service received several young horses for post mortem investigation that had died or had been euthanised. It was found that severe cyathostominosis was the cause of death. The aetiology, pathology and diagnosis of this infection is discussed. Advice is given for preventative measures and usage of anthelmintics.     

Reduction of economic losses caused by mycoplasmal pneumonia of pigs by vaccination with Respisure and by Tiamutin treatment

The possibilities and economic benefits of controlling mycoplasmal pneumonia of pigs caused by Mycoplasma hyopneumoniae by immunisation with Respisure and by Tiamutin treatment were studied. The experiment was carried out in a herd comprising 1000 sows which was free of PRRS, Aujeszky's disease, swine dysentery and leptospirosis, and the prevalence of mycoplasmal pneumonia was low because the farm had recently been restocked. Groups C1 and C2 served as untreated controls, while Groups R1 and R2 received a prestarter diet containing 100 ppm Tiamutin from the time of weaning. Piglets of Group R1 were vaccinated with Respisure vaccine once on day 69, while those of Group R2 twice, on days 65 and 80. Piglets of Groups ST1 and ST2 were fed 100 ppm Tiamutin in the diet for 7 days at the time of weaning and then at 4 months of age, while pigs of Group ST2 received such treatment also in the 6th month of life. The efficacy of treatment was analysed on the basis of the number of animals that died, were emergency slaughtered or were retarded in growth in the different groups, the body weight of animals at weaning, at 94 and 148 days of age and at the time of slaughter, their daily body weight gain, the lung lesions found in animals slaughtered from the different groups, the costs of medication and vaccination, and the cost-benefit calculations of the results. The mortality and emergency slaughter rate was 2.88% and 4.62% in Groups ST2 and ST1, respectively, 4.23% and 4.62% in Groups R2 and R1, respectively, and 8.39% and 9.44% in the control groups (C2 and C1, respectively). The rate of growth retardation was 0.48% and 2.12% in Groups R1 and R2, respectively, 1.59% and 3.46% in Groups ST1 and ST2, respectively, as compared to 8.03% and 6.55% in the control groups (C1 and C2, respectively). The severity score of lung lesions was 1.82 and 1.46 in Groups R1 and R2, 2.18 and 2.93 in Groups ST1 and ST2, and 3.83 and 4.02 in the control groups C1 and C2, respectively. The mean finishing weight of pigs was 102.4-107.8 kg and 95.2-106.6 kg in the treated groups and 94.5-98.6 kg in the control groups. The classification of pigs according to the EUROP categories showed a shift to the E and U categories in the treated groups. The average feed cost per one kg of liveweight was 77.89-82.64 Forints in the treated groups and 85.66 Forints in the control groups.     

Use of Forest Vegetation by Sheep as Recorded by Radio Telemetry

The effect of straw bedding on aggression, production and welfare in loose-housed, pregnant sows was studied in 14 commercial farms, half of them with straw bedding and the other half with a concrete floor. The herds were visited twice and agonistic behaviour was observed. Body lesions, movement disorders and production results were also recorded. There were no significant differences in aggression, body lesions or production results between herds with concrete floor and herds with straw bedding. Movement disorders were more common in herds with concrete floor than in herds with straw bedding. Herds with a high score for movement disorders tended to have a higher percentage of sows returning to oestrus than herds with a low score. Individual data showed no significant correlation between production variables and body lesion score. However, the sows that were culled had a higher mean score for body lesions than the means of both types of farms. Altogether, this study shows that straw bedding was advantageous in preventing leg problems, but the presence of straw did not affect the aggression among sows, the incidence of body lesions or the production results.     

Methane emission by growing pigs and adult sows as influenced by fermentation

The fermentation of carbohydrates in pigs' digestive system, especially in the lower gut results predominantly in short chain fatty acids, gasses as CO₂, H₂ and CH₄. Methane (CH₄) is especially interesting as it contributes to the greenhouse gas emission and thus also to the global warming. CH₄ production and its relation to fermentation in growing pigs (74 diets) as well as adult sows (36 diets), were investigated in diets varying greatly in chemical composition. All diets were assayed in digestibility and energy balance experiments in respiration chambers. Growing pigs fed diets varying in total fibre content (2.8–40%) had a CH₄ production equivalent to 0.1 to 1.3% of digested energy (DE). Non-lactating sows fed at maintenance had a CH₄ production equivalent to 0.4 to 3.3 % of DE. Intensively fed lactating sows had the lowest CH₄ production (about 0.6% of DE). In general, the production of methane depended on fibre origin, however high variation was found between animals.     

Detection of cone dysfunction induced by digoxin in dogs by multicolor electroretinography

It is difficult to detect discrete cone function with the present conventional electroretinography (ERG) examination. In this study, we developed contact electrodes with a built-in color (red (644 nm), green (525 nm), or blue (470 nm)) light source (color LED-electrode), and evaluated an experimental model of digoxin in the dog. First, 17 normal Beagle dogs were used to determine which electrode works well for color ERG measurement on dogs. Then, color ERG was performed on seven normal Beagle dogs at various points during a 14-day period of digoxin administration. A single daily dose of 0.0125 mg/kg/day, which is within the recommended oral maintenance dosage range for dogs, was administered orally for 2 weeks. Ophthalmic examination, measurement of plasma concentration of digoxin, and color ERG examination were performed. On first examination, amplitudes of all responses were significantly (P < 0.01) lower with the red, than with the blue and green electrodes during ERG recording. In ERG using the red electrode, the standard deviation was large. According to these preliminary results, the red electrode was not used in the experimental dog model with digoxin. In the digoxin administrated animals, no significant change was observed in the ophthalmic examination findings. The digoxin level increased steadily throughout the dosing period but was always within the therapeutic range for dogs. In rod ERG, no abnormalities were detected with any electrode. In standard combined ERG, decreased amplitude of the a-wave was detected with every electrode. In single flash cone ERG, prolongation of implicit time was detected by color ERG with the blue and green electrodes. In 30-Hz flicker ERG, decreased amplitude was detected only by color ERG with the blue electrode. The decreased amplitude and prolonged implicit time recovered after termination of digoxin administration. Cone dysfunction induced by digoxin in the dog was revealed by multicolor ERG using blue and green LED-electrodes. Multi-color ERG was useful for detecting cone type-specific dysfunction in the dog.     

Suppression of lymphocyte blastogenesis by sera from calves transported by road

The effect of sera from 4-h road-transported calves on mitogen-induced blastogenesis of bovine lymphocytes was investigated. Sera collected just after transportation showed a significant suppression (P less than 0.05) on blastogenesis. The immunosuppressive activity was reduced from those sera when they were treated with dextran-coated charcoal. Fractionation of the sera by ultrafiltration revealed that the activity was mainly concentrated in the fractions of molecular weights less than 50 kD.     

Sugar metabolism by Brucellae

The metabolic capabilities of the species of Brucella were originally of interest as a means of distinguishing them from each other and from other genera. Certain unusual characteristics, especially erythritol utilization, were studied in the hopes they would shed light on the pathogenicity. With the advent of modern genetic methods and genomic sequencing, it is now possible to get a good idea of the total capabilities of the organism and to do tests to confirm these deductions. Brucella appears to be a fairly normal member of the -proteobacteria, but with some differences. A few questions remain, such as whether Brucella uses the Entner–Doudoroff pathway. Some of the genes in carbohydrate utilization have been shown to be important in virulence.