| 航天搭载非洲山毛豆高鱼藤酮含量植株的筛选 |
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| 引用本文： | 吴玉东,徐汉虹,鲁冬林,陆慧,冯敏英.航天搭载非洲山毛豆高鱼藤酮含量植株的筛选[J].中国农业科学,2010,43(11):2380-2387. |
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| 作者姓名： | 吴玉东 徐汉虹 鲁冬林 陆慧 冯敏英 |
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| 作者单位： | （华南农业大学天然农药与化学生物学教育部重点实验室） |
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| 基金项目： | 广东省农业领域重点项目-农业公关，农业部行业计划，教育部产学研结合项目，广东省产学研结合项目 |
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| 摘 要： | 【目的】非洲山毛豆（Tephrosia vogelii Hook）植株原始品种鱼藤酮含量较低,其种子经航天搭载返回地面育苗后,筛选出高鱼藤酮含量的非洲山毛豆植株。【方法】筛选后的非洲山毛豆种子经卫星搭载于太空航行15天,返回地面后种植。利用HPLC和HPLC－MS对150株非洲山毛豆叶片干粉中的鱼藤酮定性和定量分析。【结果】叶片干粉中鱼藤酮含量高于2.00%的非洲山毛豆种质2株,含量在1.00%—2.00%的种质8株。在参试的非洲山毛豆种质资源中,原始品种的鱼藤酮含量仅0.4614%,21号植株的鱼藤酮含量最高,达2.3172%,比原始品种含量提高了4.02倍。【结论】航天搭载可以使非洲山毛豆种子产生变异,其植株叶片内鱼藤酮含量产生变化,筛选出的高鱼藤酮含量的非洲山毛豆特异资源,经过品质遗传改良或大量繁育后可成为提取鱼藤酮的重要材料。
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| 关 键 词： | 航天搭载 鱼藤酮 含量 筛选 高效液相色谱 |
| 收稿时间： | 2009-12-10; |
Screening of the High Rotenone Content Plant of Tophrosia vogelii Hook from Space Flight |
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| WU Yu-dong,XU Han-hong,LU Dong-lin,LU Hui,FENG Min-ying.Screening of the High Rotenone Content Plant of Tophrosia vogelii Hook from Space Flight[J].Scientia Agricultura Sinica,2010,43(11):2380-2387. |
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| Authors: | WU Yu-dong XU Han-hong LU Dong-lin LU Hui FENG Min-ying |
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| Affiliation: | (Key Lab of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University)
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| Abstract: | 【Objective】 The rotenone content of Tephrosia vogelii Hook was low in original strains and the plants cultivated by the seeds from space flight had been screened for high content of rotenone. 【Method】 The chosen seeds of Tephrosia vogelii carried by the recoverable satellite had been travelled for 15 days in space, then the seeds were sowed in the field after recovery. The qualitative and quantitative analyses on rotenone content of leaves powder of 150 Tephrosia vogelii plants were conducted by HPLC and HPLC-MS. 【Result】 Two plants with high rotenone content in the powder which was more than 2.00% and eight plants with the rotenone content of 1.00%-2.00% in the germplasm were oblained. No.21 plant had the highest rotenone content up to 2.3172% in all Tephrosia vogelii germplasm resources which was 4.02 times higher than the original strains with rotenone content only 0.4614%. 【Conclusion】High content rotenone specific resources of Tephrosia vogelii from recoverable satellite can be used as important materials for rotenone extraction after genetic improvement or massive reproduction. |
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| Keywords: | space flight rotenone content screening HPLC |
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