| 连翘酯苷对鸡IFN-α和JAK-STAT信号通路相关因子的影响 |
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| 引用本文： | 李华伟,张羽璐,张中文,夏楠,郭玮,吴国娟.连翘酯苷对鸡IFN-α和JAK-STAT信号通路相关因子的影响[J].中国农业科学,2011,44(18):3903-3908. |
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| 作者姓名： | 李华伟 张羽璐 张中文 夏楠 郭玮 吴国娟 |
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| 作者单位： | 1.北京农学院动物科学技术学院 |
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| 基金项目： | 国家自然科学基金(31172362,30771625,30972215); 北京市自然科学基金(6092004); 北京市属高校人才强教计划(PHR2009-07135) |
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| 摘 要： | 【目的】探讨连翘酯苷作用于鸡胚肾（CEK）细胞后，IFN-α的表达变化及JAK-STAT通路相关因子mRNA的表达变化，及连翘酯苷对IFN-α和JAK-STAT信号通路的影响。【方法】将连翘酯苷设为3个浓度（100、200和400 μg?mL-1），采用荧光定量RT-PCR，检测IFN-α和JAK-STAT通路相关因子的mRNA表达变化；用western blotting检测IFN-α的蛋白表达情况。【结果】与正常组相比，连翘酯苷不仅显著提高了IFN-α的表达量（P＜0.05）；而且明显上调了STAT1、JAK1、IFNAR1、IFNAR2、IRF1、IRF7的表达。【结论】连翘酯苷能够在鸡胚肾细胞（chicken embryo kidney cells，CEKs） 上诱导IFN-α的表达，且能够正向调节JAK-STAT信号通路传导。
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| 关 键 词： | 连翘酯苷 鸡胚肾细胞 IFN-α JAK-STAT real-timePCR westernblotting |
| 收稿时间： | 2010-11-09 |
Forsythiaside A Induced IFN-α and Up-Regulated Correlation Factors of JAK-STAT Signaling Pathways in vitro |
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| LI Hua-wei,ZHANG Yu-lu,ZHANG Zhong-wen,XIA Nan,GUO Wei,WU Guo-juan.Forsythiaside A Induced IFN-α and Up-Regulated Correlation Factors of JAK-STAT Signaling Pathways in vitro[J].Scientia Agricultura Sinica,2011,44(18):3903-3908. |
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| Authors: | LI Hua-wei ZHANG Yu-lu ZHANG Zhong-wen XIA Nan GUO Wei WU Guo-juan |
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| Affiliation: | LI Hua-wei,ZHANG Yu-lu,ZHANG Zhong-wen,XIA Nan,GUO Wei,WU Guo-juan(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206) |
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| Abstract: | 【Objective】 This study focused on the effect of forsythiaside A added in chick embryo kidney cells, the expression of IFN-αand the mRNA expression of correlation factors in JAK-STAT pathway. 【Methods】 In the study, three levels forsythiaside (100, 200, 400 μg?mL-1) were set, and real-time RT-PCR was used to detect the mRNA expression of IFN-α and JAK-STAT pathway related factors; western blotting was used to investigate protein expression of IFN-α. 【Result】 Compared with normal group, the expression of IFN-α increased significantly when high dose forsythiaside A was added (P＜0.05). And the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1, IRF7 was significantly increased either. 【Conclusion】Forsythiaside A induced the expression of IFN-α in chicken embryo kidney cells (CEKs), and can positively regulate the JAK-STAT signaling pathway significantly. |
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| Keywords: | forsythosideA CEKcell IFN-α JAK-STAT real-timePCR westernblotting |
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