| 小体鲟Ar-tnfsf10和Ar-tnfrsf10基因的克隆、表达和功能初步探究 |
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| 引用本文： | 展敏月,王建,范纯新.小体鲟Ar-tnfsf10和Ar-tnfrsf10基因的克隆、表达和功能初步探究[J].上海海洋大学学报,2023,32(3):472-480. |
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| 作者姓名： | 展敏月 王建 范纯新 |
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| 作者单位： | 上海海洋大学,上海海洋大学,上海海洋大学 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘 要： | 肿瘤坏死因子超家族(TNFSF)和肿瘤因子受体超家族(TNFRSF)广泛存在于各类后生动物中，参与细胞凋亡、代谢和发育等多种功能调控。为了探究TNFSF和TNFRSF在硬骨鱼中的进化，我们克隆了小体鲟Ar-tnfsf10和Ar-tnfrsf10基因，并对小体鲟Tnfsf10和Tnfrsf10序列进行进化分析以及蛋白高级结构预测。结果表明，小体鲟Tnfsf10和Tnfrsf10在进化树中位于辐鳍亚纲的基部，与四足动物的Tnfsf10和Tnfrsf10同源性较低，其三维结构与人的Tnfsf10和Tnfrsf10相近。原位杂交的结果显示：Ar-tnfsf10和Ar-tnfrsf10均在小体鲟躯干水平肌隔、头部的眶下管、前鳃盖管和腹侧吻端的神经丘处有较强表达。经Tnfrsf10特异性激活剂Bioymifi处理后，小体鲟仔鱼整体结构无显著变化，但神经丘中毛细胞显著增多。综上结果表明：小体鲟Tnfsf10和Tnfrsf10代表了硬骨鱼同源蛋白的原始形式，可能特异性参与侧线神经丘的发育。
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| 关 键 词： | 小体鲟 Tnfsf10 Tnfrsf10 表达 侧线 |
| 收稿时间： | 2021/6/1 0:00:00 |
| 修稿时间： | 2022/2/11 0:00:00 |
Cloning, expression and preliminary function analysis of Ar-tnfsf10 and Ar-tnfrsf10 genes in sterlet, Acipenser ruthenus |
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| ZHAN Minyue,WANG Jian,FAN Chunxin.Cloning, expression and preliminary function analysis of Ar-tnfsf10 and Ar-tnfrsf10 genes in sterlet, Acipenser ruthenus[J].Journal of Shanghai Ocean University,2023,32(3):472-480. |
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| Authors: | ZHAN Minyue WANG Jian FAN Chunxin |
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| Affiliation: | Shanghai Ocean University,Shanghai Ocean University,Shanghai Ocean University |
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| Abstract: | Tumor Necrosis Factor Superfamily (TNFSF) and Tumor Factor Receptor Superfamily (TNFRSF) widely exist in most kinds of metazoa and regulate cell apoptosis, metabolism and development. To explore the evolution of TNFSF and TNFRSF in teleosts, we cloned tnfsf10 and tnfrsf10 of the sterlet, and analyzed their structure and evolution. Both Tnfsf10 and Tnfrsf10 of the sterlet locate at the base of actinopterygii branch and have low homology with Tnfsf10 and Tnfrsf10 of the tetrapod, however, their tertiary structure is similar to Tnfsf10 and Tnfrsf10 of the human. Whole-mount in situ hybridization analysis showed that tnfsf10 and tnfrsf10 were mainly expressed in the horizontal muscle septum of the trunk, infraorbital canal of the head, preopercular lateral line, and neuromast of the ventral rostral. After treated with Tnfrsf10 specific activator Bioymifi, the morphology of sterlet larvae did not change significantly, but the number of hair cells in neuromast increased significantly. Our findings suggest that the Tnfsf10 and Tnfrsf10 of the sterlet represent the original form of the teleost homologous protein, which may be specifically involved in the development of the lateral line neuromast. |
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| Keywords: | sterlet Tnfsf10 Tnfrsf10 expression lateral line |
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