| Decreasing detection frequency of MITE(MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus' recently collected in southern China |
| |
| 基金项目： | This study was funded by the National Natural Science Foundation of China (31671992, 31871925), the National Key R&D Program of China (2018YFD0201500), and the Key R&D Program of Guangxi, China (Gui Ke AA18118046). |
| |
| 摘 要： | An active miniature inverted-repeat transposable element(MITE), MCLas-A, was previously identified from ‘Candidatus Liberibacter asiaticus' known to be associated with citrus Huanglongbing(HLB, yellow shoot disease). To explore the recent transposition status of MCLas-A, 389 ‘Ca. L. asiaticus' strains collected from nine regions in China were amplified using a specific primer set and three representative ‘Ca. L. asiaticus' strains were analyzed by next-generation sequencing(NGS) approach. PCR and genomic analysis showed that the entire MCLas-A was only present in 1.80%(7/389) and the jumping-out type of the MITE was predominant(81.23%) in samples tested, suggesting high frequency transposition occurred in ‘Ca. L. asiaticus' strains recently collected from China. Biological roles of transposition of the active MITE remain to be determined.
|
| 收稿时间： | 30 December 2019 |
Decreasing detection frequency of MITE (MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus’ recently collected in southern China |
| |
| Authors: | CUI Xue-jin ZENG Chun-hua LIU Ke-hong TENG Cai-ling ZHOU Chang-yong WANG Xue-feng |
| |
| Affiliation: | National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R.China |
| |
| Abstract: | An active miniature inverted-repeat transposable element (MITE), MCLas-A, was previously identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). To explore the recent transposition status of MCLas-A, 389 ‘Ca. L. asiaticus’ strains collected from nine regions in China were amplified using a specific primer set and three representative ‘Ca. L. asiaticus’ strains were analyzed by next-generation sequencing (NGS) approach. PCR and genomic analysis showed that the entire MCLas-A was only present in 1.80% (7/389) and the jumping-out type of the MITE was predominant (81.23%) in samples tested, suggesting high frequency transposition occurred in ‘Ca. L. asiaticus’ strains recently collected from China. Biological roles of transposition of the active MITE remain to be determined. |
| |
| Keywords: | Huanglongbing miniature inverted-repeat transposable element MITE |
| 本文献已被 CNKI ScienceDirect 等数据库收录！ |
| 点击此处可从《农业科学学报(英文版)》浏览原始摘要信息 |
|
点击此处可从《农业科学学报(英文版)》下载全文 |
