Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition |
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| Authors: | F Da Costa B Petton C Mingant G Bougaran C Rouxel C Quéré GH Wikfors P Soudant R Robert |
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| Affiliation: | 1. Ifremer, Laboratoire des sciences de l'Environnement Marin (UMR 6539, LEMAR), Plouzané, France;2. Novostrea Bretagne, Sarzeau, France;3. Ifremer, Laboratoire Physiologie et Biotechnologies des Algues, Nantes Cedex 3, France;4. Northeast Fisheries Science Center, NMFS, NOAA, Milford, CT, USA;5. Laboratoire des Sciences de l'Environnement Marin (UMR 6539, LEMAR), IUEM/UBO, Plouzané, France;6. Ifremer, Unité Littoral, Centre Bretagne – ZI de la Pointe du Diable, Plouzane, France |
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| Abstract: | Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell?1). Larval oyster survival and growth rate were positively correlated with 18:1n‐7 and 20:1n‐7, in storage lipids (SL), and negatively related to 14:0, 18:1n‐9, 20:1n‐9, 20:4n‐6 and trans‐22‐dehydrocholesterol in membrane lipids (ML). Surprisingly, only the essential fatty acid 20:5n‐3 in SL was correlated positively with larval survival. Correlations suggest that physiological disruption by overabundance of TAG, FFA and certain fatty acids in larvae fed T+ was largely responsible for the poor performance of these larvae. ‘High‐lipid’ strains of microalgae, without regard to qualitative lipid composition, do not always improve bivalve larval performance. |
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| Keywords: | larvae lipids oyster
Tisochrysis lutea
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