| 不同植物细胞分裂素对苹果叶片抗氧化活性及衰老的影响 |
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| 引用本文： | 辛艳伟,李六林.不同植物细胞分裂素对苹果叶片抗氧化活性及衰老的影响[J].农学学报,2015,5(12):55-58. |
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| 作者姓名： | 辛艳伟 李六林 |
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| 作者单位： | 山西农业大学资源环境学院,山西农业大学园艺学院 |
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| 基金项目： | 山西省留学人员管理委员会办公室科研资助项目“植物细胞分裂素对苹果抗氧化活性影响的研究”(2009041)。 |
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| 摘 要： | 为了提高苹果品质及其营养价值,开发功能保健苹果,以新鲜采摘的‘富士’苹果成年叶片为研究对象,使用相同浓度(1 mg/L)的激动素、玉米素和6-苄基腺嘌呤对其进行处理,每周进行1次抗氧化活性及衰老相关指标测定,连续测定1个月。结果表明,使用激动素、玉米素和6-苄基腺嘌呤处理都能提高叶片的抗氧化活性,提高叶绿素及蛋白质的含量,降低精氨酸和脯氨酸2种氨基酸的含量。其中,激动素对提高叶片抗氧化活性和叶绿素含量的效果最明显,玉米素对提高蛋白质含量和降低氨基酸含量的作用最大,6-苄基腺嘌呤对各指标的影响都处于中等水平。不同植物细胞分裂素对成年叶片的总抗氧化活性含量的提升以及对衰老的延缓,为生产富含抗氧化活性物质的功能保健苹果提供了积极的理论依据。
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| 关 键 词： | 细胞分裂素 红富士苹果 离体叶片 抗氧化活性 衰老 |
| 收稿时间： | 2015/7/7 0:00:00 |
| 修稿时间： | 2015/7/15 0:00:00 |
Effects of Different Plant Cytokinin on Antioxidant Activity and Aging of Apple Leaves |
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| Abstract: | In order to improve the quality of apple and its nutritional value, this study continuously measured the antioxidant activity and aging related indicators of the fresh detached adult leaves from Fuji apple trees every week in one month. The leaves were cultured in vitro with bottoms soaked in the solution of the same concentration of 1 mg/L kinetin, zeatin and 6-benzyl adenine. The results showed that: the total antioxidant activity of the leaves was improved by all three treatments, as well as the content of chlorophyll and the protein of the leaves. However, the content of argentine and proline declined. The kinetin treatment had the most obvious effects on improving the antioxidant activity and chlorophyll content. The zeatin treatment had the most significant effects on increasing the content of protein and reducing the content of amino acids. The 6-benzyl adenine maintained a medium-level impact. The increase of total antioxidant activity and antiaging effects through applying different plant cytokinin provided theoretical foundation for the production of functional healthy apples of rich antioxidant activity materials. |
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| Keywords: | Cytokinin Fuji Apple in vitro Leaf Antioxidant Activity Aging |
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